Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,105,157 G→T 24.1% D123Y (GAT→TAT)  opgG → osmoregulated periplasmic glucan (OPG) biosynthesis periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,105,1570GT24.1% 496.2 / 140.0 208D123Y (GAT→TAT) opgGosmoregulated periplasmic glucan (OPG) biosynthesis periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (76/82);  new base T (18/32);  total (94/114)
Fisher's exact test for biased strand distribution p-value = 1.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

Only 100 of 209 total aligned reads displayed.

TGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGC  >  CP009273/1105011‑1105306
                                                                                                                                                  |                                                                                                                                                     
tGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATa                                                                                                                                                    >  1:4641261/1‑150 (MQ=255)
tGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAACAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATa                                                                                                                                                    <  1:430690/150‑1 (MQ=255)
 gAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATAc                                                                                                                                                   >  2:303409/1‑150 (MQ=255)
 gAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATAc                                                                                                                                                   <  2:2572459/150‑1 (MQ=255)
 gAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATAc                                                                                                                                                   <  2:2571182/150‑1 (MQ=255)
  aaGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACg                                                                                                                                                  <  2:639342/150‑1 (MQ=255)
  aaGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACg                                                                                                                                                  >  1:4835474/1‑150 (MQ=255)
    gACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGt                                                                                                                                                >  2:1034996/1‑150 (MQ=255)
         cATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAg                                                                                                                                           >  2:3579797/1‑150 (MQ=255)
            tCAAACTCGAGTTCTACCCTCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGAcc                                                                                                                                        <  2:241370/150‑1 (MQ=255)
            tCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAATATACGGTAAAAGAcc                                                                                                                                        <  1:3929301/150‑1 (MQ=255)
            tCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAATATACGGTAAAAGAcc                                                                                                                                        >  1:624652/1‑150 (MQ=255)
               aaCTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTg                                                                                                                                     <  2:1742993/150‑1 (MQ=255)
                aCTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTgg                                                                                                                                    <  2:758245/150‑1 (MQ=255)
                aCTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTgg                                                                                                                                    <  2:1462933/150‑1 (MQ=255)
                aCTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTgg                                                                                                                                    <  2:1932598/150‑1 (MQ=255)
                  tCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCt                                                                                                                                  <  2:542885/150‑1 (MQ=255)
                   cGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAATATACGGTAAAAGACCTTGGCtt                                                                                                                                 >  1:3445216/1‑150 (MQ=255)
                    gAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCttt                                                                                                                                <  2:1792435/150‑1 (MQ=255)
                     aGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAACAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAATATACGGTAAAAGACCTTGGCTTTg                                                                                                                               <  2:4090964/150‑1 (MQ=255)
                       ttCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGcc                                                                                                                             <  1:3807104/150‑1 (MQ=255)
                          tACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAATATACGGTAAAAGACCTTGGCTTTGCCGGt                                                                                                                          >  1:4606082/1‑150 (MQ=255)
                           aCCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGtt                                                                                                                         >  1:424163/1‑150 (MQ=255)
                             cATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAATATACGGTAAAAGACCTTGGCTTTGCCGGtttt                                                                                                                       >  1:2620310/1‑150 (MQ=255)
                             cATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAATATACGGTAAAAGACCTTGGCTTTGCCGGtttt                                                                                                                       >  1:3049861/1‑150 (MQ=255)
                              aTCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAATATACGGTAAAAGACCTTGGCTTTGCCGGTTTTa                                                                                                                      >  1:1110780/1‑150 (MQ=255)
                              aTCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAATATACGGTAAAAGACCTTGGCTTTGCCGGTTTTa                                                                                                                      >  1:2400537/1‑150 (MQ=255)
                              aTCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTa                                                                                                                      <  2:4220020/150‑1 (MQ=255)
                                cAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTaaa                                                                                                                    <  1:263818/150‑1 (MQ=255)
                                  gggTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGt                                                                                                                  >  2:4426855/1‑150 (MQ=255)
                                  gggTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGt                                                                                                                  >  2:4429043/1‑150 (MQ=255)
                                    gTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGc                                                                                                                >  1:1151171/1‑150 (MQ=255)
                                     tATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCt                                                                                                               <  1:3385416/150‑1 (MQ=255)
                                     tATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCt                                                                                                               <  1:4864850/150‑1 (MQ=255)
                                     tATGTACTTCGATACCCCGGTCAAAATAAATGAAGGGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAATATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCt                                                                                                               <  2:4699421/150‑1 (MQ=255)
                                      aTGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCtt                                                                                                              >  1:3819270/1‑150 (MQ=255)
                                        gTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTa                                                                                                            <  1:4531426/150‑1 (MQ=255)
                                         tACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTAc                                                                                                           >  1:1243121/1‑150 (MQ=255)
                                         tACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTAc                                                                                                           <  1:839393/150‑1 (MQ=255)
                                           cTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTAccc                                                                                                         >  2:3014878/1‑150 (MQ=255)
                                             tCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGa                                                                                                       >  2:2706206/1‑150 (MQ=255)
                                                aTACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCa                                                                                                    >  1:1593924/1‑150 (MQ=255)
                                                  aCCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAAc                                                                                                  <  1:2913959/150‑1 (MQ=255)
                                                  aCCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAAc                                                                                                  <  1:2913552/150‑1 (MQ=255)
                                                   ccccGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAATATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACa                                                                                                 <  1:3229667/150‑1 (MQ=255)
                                                       ggTCAAAATAACTGAAGTGACTGCCACCGCAGTCAAACGACTCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAATATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCaa                                                                                             <  2:1497953/150‑1 (MQ=255)
                                                        gTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCaaa                                                                                            <  2:703123/150‑1 (MQ=255)
                                                         tCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAg                                                                                           <  1:3619957/150‑1 (MQ=255)
                                                          cAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGa                                                                                          <  1:1488860/150‑1 (MQ=255)
                                                           aaaaTAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGAt                                                                                         >  1:1834451/1‑150 (MQ=255)
                                                           aaaaTAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGAt                                                                                         >  1:1834726/1‑150 (MQ=255)
                                                            aaataaatGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAATATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATa                                                                                        <  1:3330391/150‑1 (MQ=255)
                                                            aaataaatGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAATATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATa                                                                                        <  1:3330501/150‑1 (MQ=255)
                                                            aaataaatGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATa                                                                                        <  1:3430907/150‑1 (MQ=255)
                                                             aataaatGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATaa                                                                                       <  2:2136199/150‑1 (MQ=255)
                                                               taaatGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAATATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATaaaa                                                                                     <  1:2132465/150‑1 (MQ=255)
                                                                aaatGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAATATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATaaaaa                                                                                    <  2:2616398/150‑1 (MQ=255)
                                                                aaatGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATaaaaa                                                                                    >  1:2251964/1‑150 (MQ=255)
                                                                 aatGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAAc                                                                                   >  1:1744241/1‑150 (MQ=255)
                                                                     aaGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAATATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATg                                                                               <  1:4845229/150‑1 (MQ=255)
                                                                          aCTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAATATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATc                                                                          <  1:3895589/150‑1 (MQ=255)
                                                                           cTGCCACCGCAGTCAAACGAATCAAATACCGCCCGTATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGAACTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCg                                                                         <  2:4395473/150‑1 (MQ=255)
                                                                              ccACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAATATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCa                                                                      <  2:909523/150‑1 (MQ=255)
                                                                              ccACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAATATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCa                                                                      <  2:3348274/150‑1 (MQ=255)
                                                                              ccACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCa                                                                      <  1:1269051/150‑1 (MQ=255)
                                                                                 ccGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCa                                                                   >  2:1998795/1‑150 (MQ=255)
                                                                                    cAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGAT‑AAAACGATGAAATCGTCAGCATGCt                                                               <  2:1117505/150‑1 (MQ=255)
                                                                                        cAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCgg                                                            <  1:409108/150‑1 (MQ=255)
                                                                                        cAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCgg                                                            >  2:2475795/1‑150 (MQ=255)
                                                                                         aaaCGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCggg                                                           >  2:752116/1‑150 (MQ=255)
                                                                                             gAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAATATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCa                                                       <  2:961829/150‑1 (MQ=255)
                                                                                             gAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCa                                                       >  1:2521734/1‑150 (MQ=255)
                                                                                              aaTCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAATATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAg                                                      <  1:3508085/150‑1 (MQ=255)
                                                                                                tCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCt                                                    <  1:3574167/150‑1 (MQ=255)
                                                                                                  aaaTACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTAt                                                  <  2:626474/150‑1 (MQ=255)
                                                                                                   aaTACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTAtt                                                 <  1:4604366/150‑1 (MQ=255)
                                                                                                     tACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTCTTTc                                               >  2:140008/1‑150 (MQ=255)
                                                                                                          cccGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGt                                          >  2:1153400/1‑150 (MQ=255)
                                                                                                           ccGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAATATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTg                                         <  2:2620310/150‑1 (MQ=255)
                                                                                                           ccGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAATATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTg                                         <  2:3049861/150‑1 (MQ=255)
                                                                                                           ccGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTg                                         <  1:1034996/150‑1 (MQ=255)
                                                                                                              gATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGAtt                                      >  2:52576/1‑150 (MQ=255)
                                                                                                                  attTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTg                                  >  2:4524562/1‑150 (MQ=255)
                                                                                                                   tttCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGc                                 <  2:4581368/150‑1 (MQ=255)
                                                                                                                           tCGGCGATGTTCAGCATGACAAATATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGcaggtcagg                         <  1:3520376/150‑1 (MQ=255)
                                                                                                                           tCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGcaggtcagg                         <  2:3514363/150‑1 (MQ=255)
                                                                                                                             ggCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGtt                       <  2:3925303/150‑1 (MQ=255)
                                                                                                                                   gTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGc                 >  1:2794310/1‑150 (MQ=255)
                                                                                                                                     tCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCt               >  1:3872888/1‑150 (MQ=255)
                                                                                                                                       aGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCttt             >  1:2483546/1‑150 (MQ=255)
                                                                                                                                       aGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCttt             <  1:1676864/150‑1 (MQ=255)
                                                                                                                                           tGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGc         >  2:1290149/1‑150 (MQ=255)
                                                                                                                                           tGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTAACCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATAGTAGAAGCTCAGGTTTATGGCCTTTCTGc         >  2:4687905/1‑150 (MQ=255)
                                                                                                                                              cAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCcg      >  2:2556510/1‑150 (MQ=255)
                                                                                                                                              cAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCcg      <  1:3510888/150‑1 (MQ=255)
                                                                                                                                               aaaGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCcgc     >  1:3705939/1‑150 (MQ=255)
                                                                                                                                               aaaGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCcgc     >  1:251649/1‑150 (MQ=255)
                                                                                                                                               aaaGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCcgc     >  1:2681747/1‑150 (MQ=255)
                                                                                                                                                 aGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTACAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCgg   <  1:3768186/150‑1 (MQ=255)
                                                                                                                                                  gATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGc  >  1:4053574/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
TGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGC  >  CP009273/1105011‑1105306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A