Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,105,607 G→T 5.3% D273Y (GAC→TAC)  opgG → osmoregulated periplasmic glucan (OPG) biosynthesis periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,105,6070GT 5.3% 1087.1 / 22.6 277D273Y (GAC→TAC) opgGosmoregulated periplasmic glucan (OPG) biosynthesis periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (132/130);  new base T (7/7);  total (140/137)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 281 total aligned reads displayed.

TGCCAGGGCGTGACACGGTTGTGGATGTGCAGTCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTT  >  CP009273/1105458‑1105756
                                                                                                                                                     |                                                                                                                                                     
tGCCAGGGCGTGACACGGTTGTGGATGTGCAGTCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACg                                                                                                                                                       >  1:776213/1‑150 (MQ=255)
        cGTGACACGGTTGTGGATGTGCAGTCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAAc                                                                                                                                               >  1:3017812/1‑150 (MQ=255)
        cGTGACACGGTTGTGGATGTGCAGTCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAAc                                                                                                                                               <  2:3759697/150‑1 (MQ=255)
        cGTGACACGGTTGTGGATGTGCAGTCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAAc                                                                                                                                               <  2:3163822/150‑1 (MQ=255)
           gACACGGTTGTGGATGTGCAGTCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGt                                                                                                                                            <  1:292110/150‑1 (MQ=255)
            acacGGTTGTGGATGTGCAGTCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGtc                                                                                                                                           <  1:1857824/150‑1 (MQ=255)
                ggTTGTGGATGTGCAGTCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGtctctc                                                                                                                                       >  2:1110896/1‑150 (MQ=255)
                ggTTGTGGATGTGCAGTCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGtctctc                                                                                                                                       <  2:2486791/150‑1 (MQ=255)
                 gTTGTGGATGTGCAGTCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTctctct                                                                                                                                      <  2:956824/150‑1 (MQ=255)
                 gTTGTGGATGTGCAGTCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTctctct                                                                                                                                      <  1:671492/150‑1 (MQ=255)
                 gTTGTGGATGTGCAGTCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTctctct                                                                                                                                      <  1:1280398/150‑1 (MQ=255)
                 gTTGTGGATGTGCAGTCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTctctct                                                                                                                                      <  2:955803/150‑1 (MQ=255)
                    gtgGATGTGCAGTCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATc                                                                                                                                   >  2:854254/1‑150 (MQ=255)
                    gtgGATGTGCAGTCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATc                                                                                                                                   >  1:2236660/1‑150 (MQ=255)
                     tgGATGTGCAGTCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACTACTCTAACGGTCTCTCTATcc                                                                                                                                  <  1:1538904/150‑1 (MQ=255)
                         tgtgCTGTCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTTTGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGc                                                                                                                              <  1:2175195/150‑1 (MQ=255)
                           tgCAGTCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCg                                                                                                                            <  1:574448/150‑1 (MQ=255)
                             cAGTCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGt                                                                                                                          >  2:3085944/1‑150 (MQ=255)
                              aGTCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTa                                                                                                                         <  2:3585920/150‑1 (MQ=255)
                              aGTCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTa                                                                                                                         <  1:2017717/150‑1 (MQ=255)
                                tctaaaaTCTATCTTCGCGATAAAGTCGGCAAATTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGAAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAAt                                                                                                                       <  1:871274/147‑1 (MQ=255)
                                 cGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTCTCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATg                                                                                                                      >  2:1451861/1‑150 (MQ=255)
                                 cGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATg                                                                                                                      <  1:83520/150‑1 (MQ=255)
                                 cGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATg                                                                                                                      >  2:1340995/1‑150 (MQ=255)
                                 cGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATg                                                                                                                      <  1:3491854/150‑1 (MQ=255)
                                   aaaaTCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGCTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGc                                                                                                                    >  1:1450218/1‑150 (MQ=255)
                                      atctatctGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGaa                                                                                                                 >  1:782822/1‑150 (MQ=255)
                                      atctatctGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGaa                                                                                                                 <  2:3937138/150‑1 (MQ=255)
                                       tctatctGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAAt                                                                                                                >  1:3954962/1‑150 (MQ=255)
                                        ctatctGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATg                                                                                                               <  2:708828/150‑1 (MQ=255)
                                            ctGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATc                                                                                                           <  2:3754518/150‑1 (MQ=255)
                                             tGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGATTGGGCCGAACCAACCGTCGCCTGCACATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAAAGGATCt                                                                                                          >  2:1867586/1‑150 (MQ=255)
                                              gcgcGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTg                                                                                                         <  2:3165563/150‑1 (MQ=255)
                                                  gATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGt                                                                                                     >  2:1425662/1‑150 (MQ=255)
                                                   aTAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTc                                                                                                    >  2:2755271/1‑150 (MQ=255)
                                                   aTAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTc                                                                                                    >  1:2638876/1‑150 (MQ=255)
                                                     aaaGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCg                                                                                                  <  1:3718903/150‑1 (MQ=255)
                                                       aGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGtt                                                                                                >  1:2829553/1‑150 (MQ=255)
                                                         tCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGa                                                                                              <  1:2007143/150‑1 (MQ=255)
                                                         tCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGa                                                                                              >  2:3662311/1‑150 (MQ=255)
                                                          cGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGaa                                                                                             <  2:3785534/150‑1 (MQ=255)
                                                          cGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGaa                                                                                             >  2:1079676/1‑150 (MQ=255)
                                                          cGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGaa                                                                                             <  2:4114963/150‑1 (MQ=255)
                                                           ggCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAAt                                                                                            <  1:167391/150‑1 (MQ=255)
                                                           ggCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAAt                                                                                            >  1:890999/1‑150 (MQ=255)
                                                             cAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATaa                                                                                          <  1:4083645/150‑1 (MQ=255)
                                                              aaaCTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAAc                                                                                         >  1:2594959/1‑150 (MQ=255)
                                                                aCTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACTACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAAccc                                                                                       >  1:775797/1‑150 (MQ=255)
                                                                 cTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCg                                                                                      <  2:3452930/150‑1 (MQ=255)
                                                                  tGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGa                                                                                     >  2:3820399/1‑150 (MQ=255)
                                                                     ggggTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACTACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAAc                                                                                  <  2:2087832/150‑1 (MQ=255)
                                                                      ggTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACa                                                                                 >  2:3286071/1‑150 (MQ=255)
                                                                       gTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACAt                                                                                >  2:2175838/1‑150 (MQ=255)
                                                                           cACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAg                                                                            >  1:131560/1‑150 (MQ=255)
                                                                           cACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAg                                                                            >  1:196123/1‑150 (MQ=255)
                                                                            aCCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACTACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGc                                                                           <  2:568536/150‑1 (MQ=255)
                                                                            aCCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGc                                                                           <  2:2540786/150‑1 (MQ=255)
                                                                                  aaCCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTcag                                                                     >  2:3965221/1‑150 (MQ=255)
                                                                                    ccAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTcagca                                                                   <  2:674360/150‑1 (MQ=255)
                                                                                     cAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACTACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTcagcag                                                                  >  2:3520980/1‑150 (MQ=255)
                                                                                     cAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTcagcag                                                                  >  2:1185084/1‑150 (MQ=255)
                                                                                     cAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTcagcag                                                                  <  2:3117580/150‑1 (MQ=255)
                                                                                      aGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCagcagc                                                                 >  1:2312137/1‑150 (MQ=255)
                                                                                           gTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTtctc                                                            >  1:3338408/1‑150 (MQ=255)
                                                                                            ttCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTcc                                                           <  2:278897/150‑1 (MQ=255)
                                                                                               cTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATg                                                        >  2:3365293/1‑150 (MQ=255)
                                                                                                 gTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGa                                                      <  2:1738377/150‑1 (MQ=255)
                                                                                                 gTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGa                                                      >  2:2335408/1‑150 (MQ=255)
                                                                                                  tttGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGaa                                                     <  2:244166/150‑1 (MQ=255)
                                                                                                    tGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGaaaa                                                   >  2:1211305/1‑150 (MQ=255)
                                                                                                     gggCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAAc                                                  <  2:2276759/150‑1 (MQ=255)
                                                                                                      ggCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAAcc                                                 >  1:2497158/1‑150 (MQ=255)
                                                                                                        ccGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCg                                               <  1:1623665/150‑1 (MQ=255)
                                                                                                         cGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGc                                              >  1:3934423/1‑150 (MQ=255)
                                                                                                         cGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGc                                              >  1:3240619/1‑150 (MQ=255)
                                                                                                          gAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCa                                             >  1:147234/1‑150 (MQ=255)
                                                                                                           aaccaaccGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCaa                                            <  1:4473/150‑1 (MQ=255)
                                                                                                           aaccaaccGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCaa                                            >  1:2823377/1‑150 (MQ=255)
                                                                                                              caaccGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGc                                         >  1:3616980/1‑150 (MQ=255)
                                                                                                              caaccGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGc                                         >  1:1118988/1‑150 (MQ=255)
                                                                                                              caaccGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGc                                         >  2:181220/1‑150 (MQ=255)
                                                                                                              caaccGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGc                                         >  1:1876279/1‑150 (MQ=255)
                                                                                                                  cGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTg                                     >  1:1023934/1‑150 (MQ=255)
                                                                                                                       ccTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTg                                >  1:1776809/1‑150 (MQ=255)
                                                                                                                        cTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGt                               >  1:1080846/1‑150 (MQ=255)
                                                                                                                         tGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGtt                              <  1:162584/150‑1 (MQ=255)
                                                                                                                            aaaTAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCa                           <  2:670228/150‑1 (MQ=255)
                                                                                                                             aaTAACTATCGTCCGGAGTTGCACGACTCTATCGGTCTCTCTATCCATGCCGGTAATGGCGACTGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAg                          <  2:1916780/150‑1 (MQ=255)
                                                                                                                              aTAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAgc                         <  1:1208497/150‑1 (MQ=255)
                                                                                                                                aaCTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAgcgc                       >  2:1667287/1‑150 (MQ=255)
                                                                                                                                 aCTATCGTCCGGCGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGcgcg                      <  1:1772614/150‑1 (MQ=255)
                                                                                                                                    aTCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTc                   <  1:3885610/150‑1 (MQ=255)
                                                                                                                                        tCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGa               <  1:339063/150‑1 (MQ=255)
                                                                                                                                         ccGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTATGTTGCAGCGCGGTCGTGAt              <  2:2019281/150‑1 (MQ=255)
                                                                                                                                          cGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGAtt             <  2:3017812/150‑1 (MQ=255)
                                                                                                                                          cGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGAtt             >  1:1098558/1‑150 (MQ=255)
                                                                                                                                            gAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTtc           <  1:3820399/150‑1 (MQ=255)
                                                                                                                                             aGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTtct          <  2:1520109/150‑1 (MQ=255)
                                                                                                                                             aGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTtct          <  1:3773837/150‑1 (MQ=255)
                                                                                                                                             aGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTtct          >  1:3361963/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGCCAGGGCGTGACACGGTTGTGGATGTGCAGTCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTT  >  CP009273/1105458‑1105756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A