Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,106,503 C→A 17.0% S62* (TCA→TAA)  opgH → membrane glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,106,5030CA17.0% 1026.9 / 123.7 349S62* (TCA→TAA) opgHmembrane glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist
Reads supporting (aligned to +/- strand):  ref base C (133/156);  new base A (27/33);  total (160/189)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 355 total aligned reads displayed.

TCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGACGCGATGTCA  >  CP009273/1106356‑1106652
                                                                                                                                                   |                                                                                                                                                     
tCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCAc                                                                                                                                                     <  2:819372/150‑1 (MQ=255)
 cgccgcAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACt                                                                                                                                                    <  1:894279/150‑1 (MQ=255)
  gccgcAAGCGAGAAAGCGGCATTGCCTAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACtt                                                                                                                                                   <  2:233583/150‑1 (MQ=255)
   ccgcAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTg                                                                                                                                                  <  1:1923384/150‑1 (MQ=255)
      cAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTg                                                                                                                                               >  2:431151/1‑150 (MQ=255)
       aaGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGa                                                                                                                                              >  2:4658809/1‑150 (MQ=255)
        aGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGAt                                                                                                                                             <  2:3127786/150‑1 (MQ=255)
        aGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGAt                                                                                                                                             <  2:4939538/150‑1 (MQ=255)
        aGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGAt                                                                                                                                             <  2:2128074/150‑1 (MQ=255)
          cGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATgg                                                                                                                                           >  2:1462258/1‑150 (MQ=255)
            agaAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGAc                                                                                                                                         >  1:3189522/1‑150 (MQ=255)
              aaaGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAg                                                                                                                                       >  1:216350/1‑150 (MQ=255)
              aaaGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGTACAg                                                                                                                                       <  2:2923816/150‑1 (MQ=255)
               aaGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGt                                                                                                                                      <  2:4081336/150‑1 (MQ=255)
                aGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGtt                                                                                                                                     <  2:2810020/150‑1 (MQ=255)
                 gCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGtta                                                                                                                                    <  2:1008099/150‑1 (MQ=255)
                    gCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGttaatt                                                                                                                                 <  1:710401/150‑1 (MQ=255)
                     cATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGttaatta                                                                                                                                >  1:2688646/1‑150 (MQ=255)
                          ccGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGGACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAgac                                                                                                                           <  2:2881896/150‑1 (MQ=255)
                          ccGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAgac                                                                                                                           <  2:3199666/150‑1 (MQ=255)
                           cGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTTAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAgacg                                                                                                                          <  2:364101/150‑1 (MQ=255)
                           cGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAacgacg                                                                                                                          <  2:195610/150‑1 (MQ=255)
                           cGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAgacg                                                                                                                          >  2:1210777/1‑150 (MQ=255)
                           cGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAgacg                                                                                                                          <  2:2623385/150‑1 (MQ=255)
                            gAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAgacga                                                                                                                         <  2:2033140/150‑1 (MQ=255)
                             aaGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGACCAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAgacgac                                                                                                                        >  2:4259203/1‑150 (MQ=255)
                              aGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGacgacg                                                                                                                       >  1:3305053/1‑150 (MQ=255)
                                 cTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAg                                                                                                                    <  1:3943386/150‑1 (MQ=255)
                                    atatCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGgc                                                                                                                 <  2:1925603/150‑1 (MQ=255)
                                    atatCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGgc                                                                                                                 <  1:1288659/150‑1 (MQ=255)
                                     tatCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCGAGCAAGGCTCGGTAAAGGCGCGTCTGGATCACGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGgcg                                                                                                                <  2:2243511/150‑1 (MQ=255)
                                     tatCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGgcg                                                                                                                <  2:3793676/150‑1 (MQ=255)
                                         cgcgCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGAt                                                                                                            >  1:2798265/1‑150 (MQ=255)
                                         cgcgCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGAt                                                                                                            <  1:4515393/150‑1 (MQ=255)
                                           cgcCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCa                                                                                                          <  1:5270108/150‑1 (MQ=255)
                                           cgcCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCa                                                                                                          <  2:1824190/150‑1 (MQ=255)
                                            gcCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGCTCAg                                                                                                         >  2:1187812/1‑150 (MQ=255)
                                               gTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTg                                                                                                      >  1:1517319/1‑150 (MQ=255)
                                               gTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGAGTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTg                                                                                                      <  2:1886194/150‑1 (MQ=255)
                                                ttCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGa                                                                                                     <  1:3568602/150‑1 (MQ=255)
                                                ttCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGa                                                                                                     <  1:4377576/150‑1 (MQ=255)
                                                ttCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGa                                                                                                     <  2:1560144/150‑1 (MQ=255)
                                                 tcatcaGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGaa                                                                                                    <  2:3587451/150‑1 (MQ=255)
                                                    tcaGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGc                                                                                                 <  2:5175467/150‑1 (MQ=255)
                                                     caGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCg                                                                                                <  2:5057578/150‑1 (MQ=255)
                                                       ggCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGAt                                                                                              >  1:472845/1‑150 (MQ=255)
                                                        gcgcTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGCCGACGAAGGGCGCGATCAGCTGAAGGCGATg                                                                                             <  1:376809/150‑1 (MQ=255)
                                                         cgcTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGc                                                                                            <  2:5036085/150‑1 (MQ=255)
                                                            tGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAg                                                                                         >  2:4421396/1‑150 (MQ=255)
                                                            tGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAg                                                                                         <  1:934690/150‑1 (MQ=255)
                                                              gATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGaa                                                                                       >  1:5140621/1‑150 (MQ=255)
                                                              gATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGaa                                                                                       >  1:2514962/1‑150 (MQ=255)
                                                               aTGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAg                                                                                      <  2:4569498/150‑1 (MQ=255)
                                                                     aaCACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAATGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAAc                                                                                >  2:3686590/1‑150 (MQ=255)
                                                                       cacCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGc                                                                              >  1:2319216/1‑150 (MQ=255)
                                                                        acCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGct                                                                             <  1:809370/150‑1 (MQ=255)
                                                                        acCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGct                                                                             >  2:4781178/1‑150 (MQ=255)
                                                                        acCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGct                                                                             <  1:1151428/150‑1 (MQ=255)
                                                                           gCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGctcct                                                                          >  1:5059441/1‑150 (MQ=255)
                                                                               cTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGAt                                                                      <  1:105490/150‑1 (MQ=255)
                                                                                tGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATg                                                                     >  2:904272/1‑150 (MQ=255)
                                                                                   gcgcGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGttt                                                                  <  1:4108060/150‑1 (MQ=255)
                                                                                      cgGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTccc                                                               <  2:1133107/150‑1 (MQ=255)
                                                                                      cgGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTccc                                                               <  2:2431807/150‑1 (MQ=255)
                                                                                       gggAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCg                                                              <  1:3621659/150‑1 (MQ=255)
                                                                                         gaggaTGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGAc                                                            >  1:4417898/1‑150 (MQ=255)
                                                                                          aggaTGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGAcc                                                           >  1:2659128/1‑150 (MQ=255)
                                                                                           ggaTGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGAccc                                                          <  1:1444472/150‑1 (MQ=255)
                                                                                             atgatTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACGGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGt                                                        >  2:838038/1‑150 (MQ=255)
                                                                                              tgatTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTg                                                       >  2:2297751/1‑150 (MQ=255)
                                                                                               gatTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTgg                                                      >  1:3979531/1‑150 (MQ=255)
                                                                                                atTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGc                                                     <  1:3368525/150‑1 (MQ=255)
                                                                                                   ccccGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTa                                                  >  2:3982663/1‑150 (MQ=255)
                                                                                                         aaGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAAcc                                            >  1:2009501/1‑150 (MQ=255)
                                                                                                          aGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCTCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAAccc                                           <  1:583176/150‑1 (MQ=255)
                                                                                                          aGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAAccc                                           <  1:431151/150‑1 (MQ=255)
                                                                                                          aGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAAccc                                           <  1:951505/150‑1 (MQ=255)
                                                                                                             cTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGt                                        <  2:3380416/150‑1 (MQ=255)
                                                                                                                ggTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAgg                                     >  1:3095128/1‑150 (MQ=255)
                                                                                                                ggTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAgg                                     >  1:1798472/1‑150 (MQ=255)
                                                                                                                ggTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAgg                                     >  2:5101158/1‑150 (MQ=255)
                                                                                                                   aaaGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGTCGCGATCAGCTGAAGGCGATGCAAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGTTAGGCCg                                  >  1:1249414/1‑150 (MQ=255)
                                                                                                                     aGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGtt                                <  2:2688646/150‑1 (MQ=255)
                                                                                                                       gcgcGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTc                              <  1:3067928/150‑1 (MQ=255)
                                                                                                                        cgcgTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCt                             <  1:1559424/150‑1 (MQ=255)
                                                                                                                            tCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGa                         >  1:2536800/1‑150 (MQ=255)
                                                                                                                               ggAACAAGCCTGGCCAGATTAACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCg                      <  1:4973032/150‑1 (MQ=255)
                                                                                                                                  aCAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCACCCCGGTAGGCCGTTTCTGGGATCGCCt                   <  1:4929588/150‑1 (MQ=255)
                                                                                                                                  aCAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCt                   <  2:1682170/150‑1 (MQ=255)
                                                                                                                                   cAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTg                  <  1:1333147/150‑1 (MQ=255)
                                                                                                                                    aaGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGc                 >  1:2975265/1‑150 (MQ=255)
                                                                                                                                    aaGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGc                 >  1:2248935/1‑150 (MQ=255)
                                                                                                                                    aaGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGc                 >  2:1284780/1‑150 (MQ=255)
                                                                                                                                     aGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCg                <  2:1620855/150‑1 (MQ=255)
                                                                                                                                         tGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGa            <  1:1860366/150‑1 (MQ=255)
                                                                                                                                           gCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGAcg          >  2:4856382/1‑150 (MQ=255)
                                                                                                                                              aGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGACGCGa       >  1:3167128/1‑150 (MQ=255)
                                                                                                                                                aTTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGACGCGATg     >  1:2897561/1‑150 (MQ=255)
                                                                                                                                                  tCACTTGCTGATGGACATTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGACGCGATGTc   <  2:418849/150‑1 (MQ=255)
                                                                                                                                                   cACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGACGCGATGTCa  <  1:4658833/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGCGTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGACGCGATGTCA  >  CP009273/1106356‑1106652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A