Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,249,716 G→T 100% R565S (CGC→AGC)  ycgV ← putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,249,7160GT95.1% 485.2 / 15.7 165R565S (CGC→AGC) ycgVputative adhesin
Reads supporting (aligned to +/- strand):  ref base G (1/7);  new base T (75/82);  total (76/89)
Fisher's exact test for biased strand distribution p-value = 7.04e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 165 total aligned reads displayed.

TCCCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTG  >  CP009273/1249571‑1249858
                                                                                                                                                 |                                                                                                                                              
 cccAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGAt                                                                                                                                             >  2:874243/1‑148 (MQ=255)
 cccAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGAt                                                                                                                                             <  1:2982845/148‑1 (MQ=255)
 cccAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGAt                                                                                                                                             <  2:1633956/148‑1 (MQ=255)
  ccAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATa                                                                                                                                            <  2:2882573/148‑1 (MQ=255)
        aGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAAt                                                                                                                                      <  2:1082437/148‑1 (MQ=255)
         gTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATa                                                                                                                                     <  1:3028027/148‑1 (MQ=255)
             cATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTg                                                                                                                                 <  2:1483762/148‑1 (MQ=255)
              aTTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGa                                                                                                                                >  1:2709283/1‑148 (MQ=255)
              aTTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGa                                                                                                                                >  1:2273589/1‑148 (MQ=255)
              aTTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGa                                                                                                                                <  2:33633/148‑1 (MQ=255)
                ttttACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTTAtt                                                                                                                              >  1:1506613/1‑148 (MQ=255)
                 tttACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTa                                                                                                                             <  1:2659833/148‑1 (MQ=255)
                 tttACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTa                                                                                                                             <  2:2353293/148‑1 (MQ=255)
                 tttACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTa                                                                                                                             <  1:901700/148‑1 (MQ=255)
                     cGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAg                                                                                                                         >  2:1066914/1‑148 (MQ=255)
                      gCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGc                                                                                                                        >  1:3329638/1‑148 (MQ=255)
                      gCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGc                                                                                                                        >  2:1693007/1‑148 (MQ=255)
                       cacaTCGTACCGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGCACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCa                                                                                                                       <  2:242906/148‑1 (MQ=255)
                       cacaTCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCa                                                                                                                       <  1:2185863/148‑1 (MQ=255)
                       cacaTCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCa                                                                                                                       <  1:213642/148‑1 (MQ=255)
                        acaTCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCac                                                                                                                      >  2:2457412/1‑148 (MQ=255)
                           tCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCactac                                                                                                                   >  2:739341/1‑148 (MQ=255)
                                 aGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGc                                                                                                             <  1:3340859/148‑1 (MQ=255)
                                   atatCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTg                                                                                                           >  1:2988529/1‑148 (MQ=255)
                                   atatCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTg                                                                                                           >  1:2568482/1‑148 (MQ=255)
                                   atatCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTg                                                                                                           <  2:655763/148‑1 (MQ=255)
                                   atatCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTg                                                                                                           <  1:181429/148‑1 (MQ=255)
                                   atatCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTg                                                                                                           >  1:1910853/1‑148 (MQ=255)
                                    tatCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGa                                                                                                          >  1:2331142/1‑148 (MQ=255)
                                    tatCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGa                                                                                                          <  2:3493805/148‑1 (MQ=255)
                                     atCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGat                                                                                                         <  1:1976280/148‑1 (MQ=255)
                                      tCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGata                                                                                                        <  1:1157107/148‑1 (MQ=255)
                                      tCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGata                                                                                                        >  1:2459764/1‑148 (MQ=255)
                                       ccccccAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGatat                                                                                                       >  1:765640/1‑148 (MQ=255)
                                       ccccccAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGatat                                                                                                       >  2:1313338/1‑148 (MQ=255)
                                        cccccAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATGTCCCGTTGTGGCCTCGCTGCCCTGGGTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATAtt                                                                                                      <  2:1876685/148‑1 (MQ=255)
                                         ccccAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTc                                                                                                     <  2:835245/148‑1 (MQ=255)
                                          cccAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCa                                                                                                    <  1:3219493/148‑1 (MQ=255)
                                          cccAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCa                                                                                                    >  1:1736929/1‑148 (MQ=255)
                                          cccAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCa                                                                                                    >  2:2178172/1‑148 (MQ=255)
                                          cccAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCa                                                                                                    >  1:971139/1‑148 (MQ=255)
                                              aCTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATaa                                                                                                <  2:599839/148‑1 (MQ=255)
                                                 cAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATc                                                                                             <  1:25515/148‑1 (MQ=255)
                                                    ccTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAACTcccc                                                                                          >  1:899230/1‑148 (MQ=255)
                                                    ccTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATcccc                                                                                          <  1:3007742/148‑1 (MQ=255)
                                                    ccTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATcccc                                                                                          <  2:1287034/148‑1 (MQ=255)
                                                      tGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCtt                                                                                        <  2:3104674/148‑1 (MQ=255)
                                                       gagaAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCttt                                                                                       <  1:1054629/148‑1 (MQ=255)
                                                       gagaAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCttt                                                                                       >  1:440979/1‑148 (MQ=255)
                                                       gagaAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCgtt                                                                                       >  1:442552/1‑148 (MQ=255)
                                                        agaagaCGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTtta                                                                                      >  1:1794232/1‑148 (MQ=255)
                                                            gaCGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTttattat                                                                                  >  1:2796729/1‑148 (MQ=255)
                                                             aCGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTttattatt                                                                                 >  1:2269164/1‑148 (MQ=255)
                                                               gcgcTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTaa                                                                               <  2:395743/148‑1 (MQ=255)
                                                               gcgcTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTaa                                                                               <  2:2224980/148‑1 (MQ=255)
                                                               gcgcTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTaa                                                                               <  1:3147222/148‑1 (MQ=255)
                                                                  cTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGc                                                                            >  2:1180764/1‑148 (MQ=255)
                                                                    gAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCa                                                                          <  2:655149/148‑1 (MQ=255)
                                                                     aaCGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCAt                                                                         >  1:2770896/1‑148 (MQ=255)
                                                                       cGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTa                                                                       <  2:3130723/148‑1 (MQ=255)
                                                                       cGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTa                                                                       >  2:1785696/1‑148 (MQ=255)
                                                                        gAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTAc                                                                      <  2:977620/148‑1 (MQ=255)
                                                                         aGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTAcc                                                                     <  1:2950424/148‑1 (MQ=255)
                                                                           gCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCt                                                                   <  1:1546465/148‑1 (MQ=255)
                                                                             cgcgCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCg                                                                 <  2:1438900/148‑1 (MQ=255)
                                                                                       gTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAacaacaa                                                       >  2:3445357/1‑147 (MQ=255)
                                                                                       gTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACAt                                                       >  1:3268301/1‑148 (MQ=255)
                                                                                       gTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACAt                                                       >  1:1138289/1‑148 (MQ=255)
                                                                                       gTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACAt                                                       <  1:1036471/148‑1 (MQ=255)
                                                                                       gTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACAt                                                       >  1:306969/1‑148 (MQ=255)
                                                                                        tGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTTATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATc                                                      <  2:3249666/148‑1 (MQ=255)
                                                                                         ggTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCa                                                     >  1:2558984/1‑148 (MQ=255)
                                                                                           tttttACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGc                                                   <  1:3491003/148‑1 (MQ=255)
                                                                                           tttttACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGc                                                   >  2:892403/1‑148 (MQ=255)
                                                                                               tACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGc                                               <  1:1882959/148‑1 (MQ=255)
                                                                                                 ccACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCat                                             <  1:513779/148‑1 (MQ=255)
                                                                                                  cACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCata                                            >  1:2555751/1‑148 (MQ=255)
                                                                                                    cTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCataat                                          >  2:2562286/1‑148 (MQ=255)
                                                                                                       tCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATaaa                                       <  2:508196/148‑1 (MQ=255)
                                                                                                       tCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATaaa                                       <  2:747502/148‑1 (MQ=255)
                                                                                                        cAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAg                                      <  2:2525817/148‑1 (MQ=255)
                                                                                                         aGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAgg                                     <  2:3070355/148‑1 (MQ=255)
                                                                                                            aCTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTAc                                  <  2:3109650/148‑1 (MQ=255)
                                                                                                              ttCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTg                                >  2:1714817/1‑148 (MQ=255)
                                                                                                              ttCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTg                                >  2:1715778/1‑148 (MQ=255)
                                                                                                                cATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGtt                              >  1:58936/1‑148 (MQ=255)
                                                                                                                  tttCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTAc                            >  1:2862032/1‑148 (MQ=255)
                                                                                                                   ttCCCGTTGTGGCCTCGATGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCACCTTTATTATTAACGCCATTACCCACGCCAACAACATCAGCACGCATAATAAAGGTAAAGTTAcc                           >  2:2594928/1‑148 (MQ=255)
                                                                                                                    tCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCg                          <  1:2550884/148‑1 (MQ=255)
                                                                                                                            tgGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGtt                  <  2:3087320/148‑1 (MQ=255)
                                                                                                                              gCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTtct                >  1:1326325/1‑148 (MQ=255)
                                                                                                                               ccTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTtctc               >  1:63566/1‑148 (MQ=255)
                                                                                                                               ccTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTtctc               <  2:2765033/148‑1 (MQ=255)
                                                                                                                                 tCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTa             >  2:372264/1‑148 (MQ=255)
                                                                                                                                   gCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACg           <  2:1282201/148‑1 (MQ=255)
                                                                                                                                    cTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGt          <  1:338276/148‑1 (MQ=255)
                                                                                                                                      gCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGttt        >  2:2480971/1‑148 (MQ=255)
                                                                                                                                       cccTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTa       <  1:3306659/148‑1 (MQ=255)
                                                                                                                                        ccTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTaa      <  1:2457412/148‑1 (MQ=255)
                                                                                                                                        ccTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTATATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTat      >  1:3155137/1‑147 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
TCCCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTG  >  CP009273/1249571‑1249858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGCTCCCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAATG  >  CP009273/1249567‑1249865
                                                                                                                                                     |                                                                                                                                                     
AATCTCCCAGTTTGTGCCATTTTTACGAACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGTCGCGCTGAACGAGGGCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTTCCCTGGTTGCT                                                                                                                                                       <  SRR19973065.1899660/150‑1 (MQ=60)
AAGCTCCCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAAATTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCT                                                                                                                                                       >  SRR19973065.1295520/1‑150 (MQ=60)
    TCCCAGTTAGTGTCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATA                                                                                                                                                   <  SRR19973065.938097/150‑1 (MQ=60)
    TCCCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATA                                                                                                                                                   >  SRR19973065.2130135/1‑150 (MQ=60)
     CCCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAG                                                                                                                                                  <  SRR19973065.3001102/150‑1 (MQ=60)
     CCCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAG                                                                                                                                                  >  SRR19973065.3106175/1‑150 (MQ=60)
          TTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAAT                                                                                                                                             <  SRR19973065.3018708/150‑1 (MQ=60)
             GTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACG                                                                                                                                          <  SRR19973065.3046584/150‑1 (MQ=60)
                CCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGA                                                                                                                                       >  SRR19973065.2288709/1‑150 (MQ=60)
                CCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGA                                                                                                                                       >  SRR19973065.2726127/1‑150 (MQ=60)
                CCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGA                                                                                                                                       >  SRR19973065.2287941/1‑150 (MQ=60)
                  ATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTTATT                                                                                                                                     >  SRR19973065.3269304/1‑150 (MQ=60)
                  ATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTTATT                                                                                                                                     >  SRR19973065.1516394/1‑150 (MQ=60)
                     TTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACC                                                                                                                                  <  SRR19973065.2676281/150‑1 (MQ=60)
                     TTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACC                                                                                                                                  <  SRR19973065.907492/150‑1 (MQ=60)
                        ACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGC                                                                                                                               >  SRR19973065.3349957/1‑150 (MQ=60)
                           CACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACT                                                                                                                            <  SRR19973065.2199886/150‑1 (MQ=60)
                           CACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACT                                                                                                                            <  SRR19973065.215354/150‑1 (MQ=60)
                           CACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACT                                                                                                                            <  SRR19973065.429951/150‑1 (MQ=60)
                           CACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACT                                                                                                                            <  SRR19973065.215258/150‑1 (MQ=60)
                                  TACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCG                                                                                                                     >  SRR19973065.1495174/1‑150 (MQ=60)
                                     AGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTG                                                                                                                  >  SRR19973065.2584519/1‑150 (MQ=60)
                                     AGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTG                                                                                                                  >  SRR19973065.3006820/1‑150 (MQ=60)
                                     AGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTG                                                                                                                  >  SRR19973065.1923403/1‑150 (MQ=60)
                                     AGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTG                                                                                                                  >  SRR19973065.1640508/1‑150 (MQ=60)
                                     AGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTG                                                                                                                  <  SRR19973065.3361271/150‑1 (MQ=60)
                                      GATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGA                                                                                                                 >  SRR19973065.2345719/1‑150 (MQ=60)
                                       ATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGAT                                                                                                                <  SRR19973065.182864/150‑1 (MQ=60)
                                        TATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATA                                                                                                               >  SRR19973065.2475265/1‑150 (MQ=60)
                                        TATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATA                                                                                                               >  SRR19973065.2474994/1‑150 (MQ=60)
                                         ATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATAT                                                                                                              <  SRR19973065.1989142/150‑1 (MQ=60)
                                         ATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATAT                                                                                                              >  SRR19973065.770730/1‑150 (MQ=60)
                                          TCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATT                                                                                                             <  SRR19973065.1164532/150‑1 (MQ=60)
                                            CCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCA                                                                                                           >  SRR19973065.977584/1‑150 (MQ=60)
                                            CCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCA                                                                                                           >  SRR19973065.1748414/1‑150 (MQ=60)
                                              CCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAAT                                                                                                         <  SRR19973065.3238845/150‑1 (MQ=60)
                                                 AACTCAACCTGAGAAGACGCGCTGAACGAGTCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTACATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATAATCAAAAAA                                                                                                      >  SRR19973065.490718/1‑150 (MQ=60)
                                                     CAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCC                                                                                                  <  SRR19973065.28008/150‑1 (MQ=60)
                                                     CAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCC                                                                                                  <  SRR19973065.25858/150‑1 (MQ=60)
                                                     CAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCC                                                                                                  <  SRR19973065.26019/150‑1 (MQ=60)
                                                      AACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAACTCCCC                                                                                                 >  SRR19973065.904992/1‑150 (MQ=60)
                                                        CCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTT                                                                                               >  SRR19973065.2492889/1‑150 (MQ=60)
                                                        CCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTT                                                                                               <  SRR19973065.3026162/150‑1 (MQ=60)
                                                        CCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTT                                                                                               <  SRR19973065.3026272/150‑1 (MQ=60)
                                                        CCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTT                                                                                               <  SRR19973065.1084064/150‑1 (MQ=60)
                                                         CTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTT                                                                                              >  SRR19973065.444033/1‑150 (MQ=60)
                                                         CTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCGTT                                                                                              >  SRR19973065.445617/1‑150 (MQ=60)
                                                          TGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTA                                                                                             >  SRR19973065.1806007/1‑150 (MQ=60)
                                                          TGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTA                                                                                             >  SRR19973065.807838/1‑150 (MQ=60)
                                                           GAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTAT                                                                                            <  SRR19973065.1061623/150‑1 (MQ=60)
                                                              AAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTAT                                                                                         >  SRR19973065.2814056/1‑150 (MQ=60)
                                                               AGACGCGCTGAACGAGGCCGCGCCGTCAGTGTTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATT                                                                                        <  SRR19973065.3359470/150‑1 (MQ=60)
                                                               AGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATT                                                                                        >  SRR19973065.2283497/1‑150 (MQ=60)
                                                                   GCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACG                                                                                    <  SRR19973065.3166338/150‑1 (MQ=60)
                                                                   GCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACG                                                                                    <  SRR19973065.3166334/150‑1 (MQ=60)
                                                                       TGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCAT                                                                                >  SRR19973065.2788106/1‑150 (MQ=60)
                                                                        GAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATT                                                                               <  SRR19973065.1902054/150‑1 (MQ=60)
                                                                         AACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTA                                                                              >  SRR19973065.2805603/1‑150 (MQ=60)
                                                                             AGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCT                                                                          <  SRR19973065.2968396/150‑1 (MQ=60)
                                                                             AGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCT                                                                          <  SRR19973065.2968391/150‑1 (MQ=60)
                                                                               GCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCG                                                                        <  SRR19973065.1556698/150‑1 (MQ=60)
                                                                                        TCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACA                                                               <  SRR19973065.2285489/150‑1 (MQ=60)
                                                                                         CAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACAT                                                              >  SRR19973065.309011/1‑150 (MQ=60)
                                                                                         CAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACAT                                                              >  SRR19973065.1145659/1‑150 (MQ=60)
                                                                                         CAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACAT                                                              >  SRR19973065.3288123/1‑150 (MQ=60)
                                                                                           GTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCA                                                            <  SRR19973065.1043337/150‑1 (MQ=60)
                                                                                           GTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCA                                                            >  SRR19973065.2574988/1‑150 (MQ=60)
                                                                                               TTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCAC                                                        <  SRR19973065.3512229/150‑1 (MQ=60)
                                                                                                   TACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCAT                                                    <  SRR19973065.1895355/150‑1 (MQ=60)
                                                                                                    ACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATA                                                   >  SRR19973065.2571738/1‑150 (MQ=60)
                                                                                                    ACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATA                                                   <  SRR19973065.2960009/150‑1 (MQ=60)
                                                                                                    ACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATA                                                   >  SRR19973065.2571414/1‑150 (MQ=60)
                                                                                                     CCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAA                                                  <  SRR19973065.517180/150‑1 (MQ=60)
                                                                                                      CACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAAT                                                 <  SRR19973065.3289056/150‑1 (MQ=60)
                                                                                                          GTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAG                                             <  SRR19973065.3478501/150‑1 (MQ=60)
                                                                                                             AGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTA                                          <  SRR19973065.262444/150‑1 (MQ=60)
                                                                                                                  TTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTT                                     >  SRR19973065.1847781/1‑150 (MQ=60)
                                                                                                                  TTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTT                                     >  SRR19973065.59566/1‑150 (MQ=60)
                                                                                                                    CATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTAC                                   >  SRR19973065.2879602/1‑150 (MQ=60)
                                                                                                                       TTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGC                                >  SRR19973065.1768419/1‑150 (MQ=60)
                                                                                                                        TCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCT                               <  SRR19973065.2566851/150‑1 (MQ=60)
                                                                                                                        TCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCT                               >  SRR19973065.558889/1‑150 (MQ=60)
                                                                                                                        TCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCT                               >  SRR19973065.662819/1‑150 (MQ=60)
                                                                                                                                TGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCT                       >  SRR19973065.1335062/1‑150 (MQ=60)
                                                                                                                                TGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCT                       >  SRR19973065.1335090/1‑150 (MQ=60)
                                                                                                                                 GGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTC                      >  SRR19973065.64246/1‑150 (MQ=60)
                                                                                                                                        CTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTT               <  SRR19973065.340636/150‑1 (MQ=60)
                                                                                                                                          GCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTATATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAT             >  SRR19973065.3174273/1‑150 (MQ=60)
                                                                                                                                           CCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAT            <  SRR19973065.3326809/150‑1 (MQ=60)
                                                                                                                                            CCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATG           <  SRR19973065.2472900/150‑1 (MQ=60)
                                                                                                                                                  TGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATCAAGGTACTGTTACCGCTCAGGTTCTCTACGTATAATGTGCCAA     >  SRR19973065.1920760/1‑150 (MQ=60)
                                                                                                                                                     TGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAATG  <  SRR19973065.2578303/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
AAGCTCCCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAATG  >  CP009273/1249567‑1249865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑