Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,249,716 G→T 80.2% R565S (CGC→AGC)  ycgV ← putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,249,7160GT80.2% 416.0 / 124.1 217R565S (CGC→AGC) ycgVputative adhesin
Reads supporting (aligned to +/- strand):  ref base G (23/20);  new base T (77/97);  total (100/117)
Fisher's exact test for biased strand distribution p-value = 3.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 217 total aligned reads displayed.

GCTCCCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAA  >  CP009273/1249569‑1249862
                                                                                                                                                   |                                                                                                                                                  
gCTCCCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGa                                                                                                                                                  <  2:4794148/150‑1 (MQ=255)
 cTCCCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGAt                                                                                                                                                 >  1:1997014/1‑150 (MQ=255)
  tCCCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATa                                                                                                                                                >  2:1149145/1‑150 (MQ=255)
     cAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGcc                                                                                                                                             <  1:4152934/150‑1 (MQ=255)
      aGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCa                                                                                                                                            >  1:2696595/1‑150 (MQ=255)
      aGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCa                                                                                                                                            >  2:4640301/1‑150 (MQ=255)
         tAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATa                                                                                                                                         <  2:3120367/150‑1 (MQ=255)
          aGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATAc                                                                                                                                        <  1:4738559/150‑1 (MQ=255)
           gTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACg                                                                                                                                       >  1:1918195/1‑150 (MQ=255)
             gCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTAAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTg                                                                                                                                     <  2:1144017/150‑1 (MQ=255)
              ccATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGa                                                                                                                                    >  2:2245474/1‑150 (MQ=255)
                  ttttACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTAc                                                                                                                                >  1:889747/1‑150 (MQ=255)
                    ttACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCa                                                                                                                              >  1:2974978/1‑150 (MQ=255)
                      aCGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGc                                                                                                                            <  1:1416591/150‑1 (MQ=255)
                       cGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCa                                                                                                                           >  2:1656744/1‑150 (MQ=255)
                        gCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCac                                                                                                                          <  2:3689362/150‑1 (MQ=255)
                         cacaTCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCact                                                                                                                         >  2:4157753/1‑150 (MQ=255)
                          acaTCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCacta                                                                                                                        <  2:3786142/150‑1 (MQ=255)
                               gTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGAGATTACCAGCACTACTccc                                                                                                                   >  1:4395862/1‑150 (MQ=255)
                                 aCAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGc                                                                                                                 >  2:2755293/1‑150 (MQ=255)
                                  cAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCt                                                                                                                <  2:3334193/150‑1 (MQ=255)
                                   aGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTg                                                                                                               >  1:1913298/1‑150 (MQ=255)
                                     atatCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGat                                                                                                             >  1:4221836/1‑150 (MQ=255)
                                       atCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGatat                                                                                                           >  2:72038/1‑150 (MQ=255)
                                         ccccccAACTCAACCTGAGAATACGCGCTGAACGTGGCAGCTCCGTCAGTGGTTTGTACCACTGTCAGAACTTCATTTCCCGTTTTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTc                                                                                                         <  1:4413568/150‑1 (MQ=255)
                                         ccccccAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTc                                                                                                         <  2:2892497/150‑1 (MQ=255)
                                         ccccccAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTc                                                                                                         <  2:2024504/150‑1 (MQ=255)
                                           ccccAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCaa                                                                                                       >  2:3934470/1‑150 (MQ=255)
                                             ccAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATa                                                                                                     >  1:4673763/1‑150 (MQ=255)
                                               aaCTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATaaa                                                                                                   <  2:316553/150‑1 (MQ=255)
                                                 cTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATc                                                                                                 <  2:2433129/150‑1 (MQ=255)
                                                    aaCCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATGTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATcccc                                                                                              <  1:3309620/150‑1 (MQ=255)
                                                      ccTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCtt                                                                                            <  2:4867782/150‑1 (MQ=255)
                                                            aagaCGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTttattat                                                                                      <  2:3076792/150‑1 (MQ=255)
                                                              gaCGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTttattatta                                                                                    <  2:1107248/150‑1 (MQ=255)
                                                                cgcgCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAAc                                                                                  >  2:3821816/1‑150 (MQ=255)
                                                                  cgcTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGc                                                                                >  2:1906376/1‑150 (MQ=255)
                                                                    cTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCa                                                                              <  1:2440117/150‑1 (MQ=255)
                                                                     tGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCAt                                                                             >  1:3862360/1‑150 (MQ=255)
                                                                     tGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCAt                                                                             >  2:4484460/1‑150 (MQ=255)
                                                                     tGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCAt                                                                             <  1:3991679/150‑1 (MQ=255)
                                                                      gAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCAtt                                                                            >  1:214554/1‑150 (MQ=255)
                                                                      gAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCAtt                                                                            <  1:1223669/150‑1 (MQ=255)
                                                                      gAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCAtt                                                                            <  2:1754872/150‑1 (MQ=255)
                                                                         cGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTAcc                                                                         <  1:1719616/150‑1 (MQ=255)
                                                                          gAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTAccc                                                                        >  1:3106286/1‑150 (MQ=255)
                                                                           aGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCt                                                                       <  2:3257041/150‑1 (MQ=255)
                                                                            ggCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTc                                                                      <  2:5136921/150‑1 (MQ=255)
                                                                               cgcgCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGcc                                                                   >  1:1057835/1‑150 (MQ=255)
                                                                                gcgcCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCca                                                                  <  2:2069093/150‑1 (MQ=255)
                                                                                  gcCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCcaac                                                                <  2:2402380/150‑1 (MQ=255)
                                                                                    cGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCcaacaa                                                              >  1:506045/1‑150 (MQ=255)
                                                                                    cGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCcaacaa                                                              >  1:3559443/1‑150 (MQ=255)
                                                                                     gTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCaacaac                                                             >  1:3922712/1‑150 (MQ=255)
                                                                                      tCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAacaaca                                                            >  1:3604307/1‑150 (MQ=255)
                                                                                             tttatACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCAc                                                     <  1:4820479/150‑1 (MQ=255)
                                                                                                ttACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCa                                                  >  1:3779631/1‑150 (MQ=255)
                                                                                                 tACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCat                                                 <  1:106399/150‑1 (MQ=255)
                                                                                                       tGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATaaa                                           <  2:1456758/150‑1 (MQ=255)
                                                                                                         tCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAgg                                         >  1:876240/1‑150 (MQ=255)
                                                                                                         tCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATCATAAAgg                                         >  2:2406400/1‑150 (MQ=255)
                                                                                                         tCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAgg                                         >  2:1395835/1‑150 (MQ=255)
                                                                                                         tCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAgg                                         >  2:4970479/1‑150 (MQ=255)
                                                                                                          cAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGt                                        >  1:1485806/1‑150 (MQ=255)
                                                                                                           aGAACTTCAGTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTa                                       <  2:1550250/150‑1 (MQ=255)
                                                                                                                ttCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGtt                                  >  1:687651/1‑150 (MQ=255)
                                                                                                                ttCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGtt                                  <  1:4640301/150‑1 (MQ=255)
                                                                                                                 tCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTa                                 >  2:4032732/1‑150 (MQ=255)
                                                                                                                   aTTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTAcc                               <  1:2245474/150‑1 (MQ=255)
                                                                                                                   aTTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTAcc                               <  2:1492119/150‑1 (MQ=255)
                                                                                                                   aTTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTAcc                               >  2:4122494/1‑150 (MQ=255)
                                                                                                                    tttCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCg                              >  2:1289149/1‑150 (MQ=255)
                                                                                                                     ttCCCGTTGTGGCCTCGCTGCCCTGGTTGCTTATAGCCATTACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCATCGCCAACAACATCAGCAAGCATAATAAAGGTACTGTTACCGc                             <  2:1239749/150‑1 (MQ=255)
                                                                                                                       cccGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTc                           >  1:4051677/1‑150 (MQ=255)
                                                                                                                        ccGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCa                          <  2:3272231/150‑1 (MQ=255)
                                                                                                                         cGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAg                         <  1:881782/150‑1 (MQ=255)
                                                                                                                         cGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTAATGTTACCGCTCAg                         >  1:723471/1‑150 (MQ=255)
                                                                                                                          gTTGTGGCCTCGTTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAgg                        <  1:866969/150‑1 (MQ=255)
                                                                                                                            tgtgGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGtt                      <  2:889060/150‑1 (MQ=255)
                                                                                                                            tgtgGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGtt                      <  2:1172824/150‑1 (MQ=255)
                                                                                                                             gtgGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTtc                     <  1:3436468/150‑1 (MQ=255)
                                                                                                                              tgGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTtct                    <  2:585438/150‑1 (MQ=255)
                                                                                                                              tgGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTtct                    <  2:2696957/150‑1 (MQ=255)
                                                                                                                               ggCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTtctc                   <  1:259852/150‑1 (MQ=255)
                                                                                                                               ggCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTtctc                   >  2:1109677/1‑150 (MQ=255)
                                                                                                                                gCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTctct                  <  1:4856756/150‑1 (MQ=255)
                                                                                                                                 ccTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTa                 <  1:4587105/150‑1 (MQ=255)
                                                                                                                                 ccTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTa                 <  2:4674043/150‑1 (MQ=255)
                                                                                                                                 ccTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTa                 <  2:2970586/150‑1 (MQ=255)
                                                                                                                                  cTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTAc                >  2:2571585/1‑150 (MQ=255)
                                                                                                                                   tCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACg               >  1:643856/1‑150 (MQ=255)
                                                                                                                                   tCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACg               >  1:4334516/1‑150 (MQ=255)
                                                                                                                                     gCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGtt             >  1:1085703/1‑150 (MQ=255)
                                                                                                                                     gCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGtt             >  1:4369609/1‑150 (MQ=255)
                                                                                                                                         cccTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAt         <  1:4688580/150‑1 (MQ=255)
                                                                                                                                          ccTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAtg        <  2:5064714/150‑1 (MQ=255)
                                                                                                                                            tGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAtgtg      >  2:4318065/1‑150 (MQ=255)
                                                                                                                                             ggTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTgg     <  2:3648060/150‑1 (MQ=255)
                                                                                                                                               ttGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCa   >  1:3170277/1‑150 (MQ=255)
                                                                                                                                                tGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCaa  <  2:3559443/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCTCCCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAA  >  CP009273/1249569‑1249862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCTCCCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAATG  >  CP009273/1249568‑1249865
                                                                                                                                                    |                                                                                                                                                     
AGCTCCCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTG                                                                                                                                                      <  SRR19973063.3455161/150‑1 (MQ=60)
 GCTCCCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGA                                                                                                                                                     <  SRR19973063.3272154/150‑1 (MQ=60)
  CTCCCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGAT                                                                                                                                                    >  SRR19973063.2003253/1‑150 (MQ=60)
     CCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGC                                                                                                                                                 >  SRR19973063.1461200/1‑150 (MQ=60)
      CAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCC                                                                                                                                                <  SRR19973063.4166216/150‑1 (MQ=60)
      CAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCC                                                                                                                                                >  SRR19973063.3260079/1‑150 (MQ=60)
       AGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCA                                                                                                                                               >  SRR19973063.2705260/1‑150 (MQ=60)
       AGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCA                                                                                                                                               >  SRR19973063.2705289/1‑150 (MQ=60)
           AGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATAC                                                                                                                                           <  SRR19973063.4753654/150‑1 (MQ=60)
            GTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACG                                                                                                                                          >  SRR19973063.1924186/1‑150 (MQ=60)
            GTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACG                                                                                                                                          >  SRR19973063.1924197/1‑150 (MQ=60)
            GTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACG                                                                                                                                          >  SRR19973063.4310189/1‑150 (MQ=60)
            GTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACG                                                                                                                                          <  SRR19973063.2220533/150‑1 (MQ=60)
                  TTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTA                                                                                                                                    <  SRR19973063.4693262/150‑1 (MQ=60)
                   TTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTAC                                                                                                                                   <  SRR19973063.4625070/150‑1 (MQ=60)
                   TTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTAC                                                                                                                                   >  SRR19973063.4677824/1‑150 (MQ=60)
                   TTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTAC                                                                                                                                   >  SRR19973063.892323/1‑150 (MQ=60)
                     TTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCA                                                                                                                                 >  SRR19973063.2984486/1‑150 (MQ=60)
                       ACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGC                                                                                                                               <  SRR19973063.1420896/150‑1 (MQ=60)
                                GTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGAGATTACCAGCACTACTCCC                                                                                                                      >  SRR19973063.4409943/1‑150 (MQ=60)
                                    AGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTG                                                                                                                  >  SRR19973063.1919284/1‑150 (MQ=60)
                                    AGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTG                                                                                                                  <  SRR19973063.4133287/150‑1 (MQ=60)
                                      ATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGAT                                                                                                                >  SRR19973063.4414544/1‑150 (MQ=60)
                                      ATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGAT                                                                                                                >  SRR19973063.4235296/1‑150 (MQ=60)
                                      ATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGAT                                                                                                                <  SRR19973063.4934476/150‑1 (MQ=60)
                                        ATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATAT                                                                                                              <  SRR19973063.2428561/150‑1 (MQ=60)
                                          CCCCCCAACTCAACCTGAGAATACGCGCTGAACGTGGCAGCTCCGTCAGTGGTTTGTACCACTGTCAGAACTTCATTTCCCGTTTTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTC                                                                                                            <  SRR19973063.4427727/150‑1 (MQ=60)
                                           CCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCA                                                                                                           >  SRR19973063.3692843/1‑150 (MQ=60)
                                            CCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAA                                                                                                          >  SRR19973063.824353/1‑150 (MQ=60)
                                              CCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATA                                                                                                        >  SRR19973063.4688950/1‑150 (MQ=60)
                                              CCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACAAGCACTACTCCCGCTGATATTCAATA                                                                                                        >  SRR19973063.4688670/1‑150 (MQ=60)
                                                 ACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAAT                                                                                                     >  SRR19973063.2990808/1‑150 (MQ=60)
                                                   TCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCC                                                                                                   <  SRR19973063.2878858/150‑1 (MQ=60)
                                                     AACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCC                                                                                                 >  SRR19973063.3978258/1‑150 (MQ=60)
                                                     AACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATGTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCC                                                                                                 <  SRR19973063.3320207/150‑1 (MQ=60)
                                                       CCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTT                                                                                               <  SRR19973063.88766/150‑1 (MQ=60)
                                                       CCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTT                                                                                               >  SRR19973063.4358879/1‑150 (MQ=60)
                                                        CTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTT                                                                                              <  SRR19973063.1140446/150‑1 (MQ=60)
                                                         TGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTA                                                                                             <  SRR19973063.4736537/150‑1 (MQ=60)
                                                            GAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATGTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTA                                                                                          <  SRR19973063.70871/150‑1 (MQ=60)
                                                               GACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTA                                                                                       <  SRR19973063.4762365/150‑1 (MQ=60)
                                                                  GCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACG                                                                                    <  SRR19973063.88079/150‑1 (MQ=60)
                                                                     CTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCA                                                                                 <  SRR19973063.2447916/150‑1 (MQ=60)
                                                                      TGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCAT                                                                                >  SRR19973063.681735/1‑150 (MQ=60)
                                                                      TGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCAT                                                                                >  SRR19973063.3874647/1‑150 (MQ=60)
                                                                      TGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCAT                                                                                <  SRR19973063.4004476/150‑1 (MQ=60)
                                                                       GAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATT                                                                               >  SRR19973063.215198/1‑150 (MQ=60)
                                                                       GAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATT                                                                               >  SRR19973063.3881819/1‑150 (MQ=60)
                                                                       GAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATT                                                                               <  SRR19973063.1227329/150‑1 (MQ=60)
                                                                        AACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTA                                                                              >  SRR19973063.3152547/1‑150 (MQ=60)
                                                                          CGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACC                                                                            <  SRR19973063.1724863/150‑1 (MQ=60)
                                                                           GAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCC                                                                           >  SRR19973063.519829/1‑150 (MQ=60)
                                                                           GAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCC                                                                           >  SRR19973063.3116280/1‑150 (MQ=60)
                                                                             GGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATACATCCCCTTTATTATTAACGCCATTACCCTC                                                                         <  SRR19973063.4366695/150‑1 (MQ=60)
                                                                             GGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTC                                                                         <  SRR19973063.2043148/150‑1 (MQ=60)
                                                                             GGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTC                                                                         >  SRR19973063.1596918/1‑150 (MQ=60)
                                                                                CGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCC                                                                      >  SRR19973063.1061039/1‑150 (MQ=60)
                                                                                    CCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACA                                                                  <  SRR19973063.357274/150‑1 (MQ=60)
                                                                                     CGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAA                                                                 >  SRR19973063.507512/1‑150 (MQ=60)
                                                                                     CGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAA                                                                 >  SRR19973063.3570856/1‑150 (MQ=60)
                                                                                      GTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAAC                                                                >  SRR19973063.3935227/1‑150 (MQ=60)
                                                                                       TCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACA                                                               >  SRR19973063.3615874/1‑150 (MQ=60)
                                                                                            GGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACTTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGC                                                          <  SRR19973063.1062125/150‑1 (MQ=60)
                                                                                              TTTATACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCAC                                                        <  SRR19973063.4835913/150‑1 (MQ=60)
                                                                                                 TTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCA                                                     >  SRR19973063.3791699/1‑150 (MQ=60)
                                                                                                  TACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCAT                                                    <  SRR19973063.106759/150‑1 (MQ=60)
                                                                                                  TACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCAT                                                    <  SRR19973063.107020/150‑1 (MQ=60)
                                                                                                    CCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAA                                                  <  SRR19973063.4396434/150‑1 (MQ=60)
                                                                                                    CCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAA                                                  <  SRR19973063.1974152/150‑1 (MQ=60)
                                                                                                    CCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAA                                                  <  SRR19973063.2337926/150‑1 (MQ=60)
                                                                                                          TCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGG                                            >  SRR19973063.878762/1‑150 (MQ=60)
                                                                                                           CAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGT                                           >  SRR19973063.1490360/1‑150 (MQ=60)
                                                                                                             GAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTAC                                         <  SRR19973063.3799751/150‑1 (MQ=60)
                                                                                                               ACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTG                                       >  SRR19973063.2496263/1‑150 (MQ=60)
                                                                                                                 TTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTT                                     >  SRR19973063.689667/1‑150 (MQ=60)
                                                                                                                 TTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTT                                     <  SRR19973063.4655125/150‑1 (MQ=60)
                                                                                                                  TCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTA                                    <  SRR19973063.4166466/150‑1 (MQ=60)
                                                                                                                    ATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACC                                  <  SRR19973063.2252608/150‑1 (MQ=60)
                                                                                                                    ATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACC                                  >  SRR19973063.960344/1‑150 (MQ=60)
                                                                                                                        CCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTC                              >  SRR19973063.4064666/1‑150 (MQ=60)
                                                                                                                        CCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTC                              >  SRR19973063.4064643/1‑150 (MQ=60)
                                                                                                                          CGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAG                            <  SRR19973063.884329/150‑1 (MQ=60)
                                                                                                                          CGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTAATGTTACCGCTCAG                            >  SRR19973063.725597/1‑150 (MQ=60)
                                                                                                                           GTTGTGGCCTCGTTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGG                           <  SRR19973063.869855/150‑1 (MQ=60)
                                                                                                                           GTTGTGGCCTCGTTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGG                           <  SRR19973063.869464/150‑1 (MQ=60)
                                                                                                                             TGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTT                         <  SRR19973063.1291670/150‑1 (MQ=60)
                                                                                                                              GTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTC                        <  SRR19973063.3447398/150‑1 (MQ=60)
                                                                                                                                GGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTC                      <  SRR19973063.260629/150‑1 (MQ=60)
                                                                                                                                 GCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCT                     <  SRR19973063.4872403/150‑1 (MQ=60)
                                                                                                                                  CCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTA                    <  SRR19973063.4601782/150‑1 (MQ=60)
                                                                                                                                   CTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTAC                   <  SRR19973063.3325399/150‑1 (MQ=60)
                                                                                                                                    TCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACG                  >  SRR19973063.645746/1‑150 (MQ=60)
                                                                                                                                    TCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACG                  >  SRR19973063.4348330/1‑150 (MQ=60)
                                                                                                                                      GCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTT                >  SRR19973063.1089009/1‑150 (MQ=60)
                                                                                                                                      GCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTT                >  SRR19973063.4383588/1‑150 (MQ=60)
                                                                                                                                       CTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTT               >  SRR19973063.1839953/1‑150 (MQ=60)
                                                                                                                                       CTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTT               <  SRR19973063.4869602/150‑1 (MQ=60)
                                                                                                                                        TGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTA              >  SRR19973063.3465624/1‑150 (MQ=60)
                                                                                                                                          CCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAT            <  SRR19973063.4703526/150‑1 (MQ=60)
                                                                                                                                             TGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTG         <  SRR19973063.1647734/150‑1 (MQ=60)
                                                                                                                                             TGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTG         >  SRR19973063.652239/1‑150 (MQ=60)
                                                                                                                                               GTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGC       <  SRR19973063.3910363/150‑1 (MQ=60)
                                                                                                                                                TTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCA      >  SRR19973063.3180483/1‑150 (MQ=60)
                                                                                                                                                 TGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAA     <  SRR19973063.4467045/150‑1 (MQ=60)
                                                                                                                                                    TGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAATG  >  SRR19973063.23345/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
AGCTCCCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAATG  >  CP009273/1249568‑1249865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑