Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,380,856 C→T 100% intergenic (+27/‑121) ycjF → / → tyrR UPF0283 family inner membrane protein/aromatic amino acid biosynthesis and transport regulon transcriptional regulator; autorepressor; ATPase; phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,380,8560CT91.4% 574.8 / 37.7 186intergenic (+27/‑121)ycjF/tyrRUPF0283 family inner membrane protein/aromatic amino acid biosynthesis and transport regulon transcriptional regulator; autorepressor; ATPase; phosphatase
Reads supporting (aligned to +/- strand):  ref base C (8/8);  new base T (93/77);  total (101/85)
Fisher's exact test for biased strand distribution p-value = 7.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 187 total aligned reads displayed.

TTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGA  >  CP009273/1380711‑1380999
                                                                                                                                                 |                                                                                                                                                
tttGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCtt                                                                                                                                                <  2:2473261/148‑1 (MQ=255)
             gCCGTGGATTGACGATGACAAACCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGc                                                                                                                                   >  1:2277980/1‑148 (MQ=255)
                  ggATTGACGATGACAAACCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGtt                                                                                                                              <  2:2008139/148‑1 (MQ=255)
                   gATTGACGATGACAACCCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGttt                                                                                                                             <  2:40166/148‑1 (MQ=255)
                    aTTGACGATGACAAACCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGtttt                                                                                                                            >  2:290850/1‑148 (MQ=255)
                        aCGATGACAAACCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAGAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGACGTGCCCGTGTTTCCg                                                                                                                        >  2:3215208/1‑148 (MQ=255)
                         cGATGACAAACCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGt                                                                                                                       <  1:800056/148‑1 (MQ=255)
                            tGACAAACCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCtt                                                                                                                    >  2:71932/1‑148 (MQ=255)
                            tGACAAACCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCtt                                                                                                                    >  2:2646909/1‑148 (MQ=255)
                             gACAAACCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCttt                                                                                                                   >  2:2346706/1‑148 (MQ=255)
                             gACAAACCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCttt                                                                                                                   >  2:583791/1‑148 (MQ=255)
                               cAAACCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTtgt                                                                                                                 <  2:547461/148‑1 (MQ=255)
                               cAAACCTCGCCTCGGGGATGTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTtgt                                                                                                                 <  1:405957/148‑1 (MQ=255)
                                 aaCCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTgtgt                                                                                                               >  2:186297/1‑148 (MQ=255)
                                 aaCCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTgtgt                                                                                                               >  2:1102442/1‑148 (MQ=255)
                                  aCCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTc                                                                                                              >  2:3136304/1‑148 (MQ=255)
                                  aCCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTc                                                                                                              >  2:1391272/1‑148 (MQ=255)
                                    ctcgcctcgGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCaa                                                                                                            <  2:3117540/148‑1 (MQ=255)
                                    ctcgcctcgGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCaa                                                                                                            <  2:2057547/148‑1 (MQ=255)
                                     tcgcctcgGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAAt                                                                                                           >  2:2096769/1‑148 (MQ=255)
                                     tcgcctcgGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAAt                                                                                                           >  2:826766/1‑148 (MQ=255)
                                      cgcctcgGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATg                                                                                                          <  1:145892/148‑1 (MQ=255)
                                        cctcgGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGAt                                                                                                        <  2:3009550/148‑1 (MQ=255)
                                          tcgGGGATTTCCGTCGTCAGGTTATCGGTCATTTTTAAGAAACGCTTCAAAAAGGCAACACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGAttg                                                                                                      <  1:1516845/148‑1 (MQ=255)
                                          tcgGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGAttg                                                                                                      >  1:1517222/1‑148 (MQ=255)
                                           cgGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGAttgt                                                                                                     <  1:1251394/148‑1 (MQ=255)
                                              ggATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGa                                                                                                  >  2:1030133/1‑148 (MQ=255)
                                              ggATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGa                                                                                                  >  2:3293655/1‑148 (MQ=255)
                                                 tttCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAg                                                                                               >  1:2558601/1‑148 (MQ=255)
                                                   tCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGaa                                                                                             >  1:168395/1‑148 (MQ=255)
                                                    ccGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGaaa                                                                                            >  1:3309414/1‑148 (MQ=255)
                                                        cgtcAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCtt                                                                                        >  1:2116118/1‑148 (MQ=255)
                                                          tcAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTcc                                                                                      >  1:1499051/1‑148 (MQ=255)
                                                          tcAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTcc                                                                                      <  1:595798/148‑1 (MQ=255)
                                                            aGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTg                                                                                    <  1:1357109/148‑1 (MQ=255)
                                                             gCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGc                                                                                   >  2:1563752/1‑148 (MQ=255)
                                                                 aTCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATc                                                                               >  1:3037245/1‑148 (MQ=255)
                                                                     gTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑aaa                                                                          >  1:2098613/1‑148 (MQ=255)
                                                                      tCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAAt                                                                         >  2:1766501/1‑148 (MQ=255)
                                                                      tCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAAt                                                                         >  1:698277/1‑148 (MQ=255)
                                                                         ggTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAgt                                                                      <  2:1542712/148‑1 (MQ=255)
                                                                          gTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAgtg                                                                     >  2:141380/1‑148 (MQ=255)
                                                                          gTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAgtg                                                                     >  1:493263/1‑148 (MQ=255)
                                                                          gTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAgtg                                                                     >  1:809122/1‑148 (MQ=255)
                                                                            gaaagaaaCGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTc                                                                   <  1:2411183/148‑1 (MQ=255)
                                                                             aaagaaaCGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑CAATAGTGTCa                                                                  >  2:643938/1‑148 (MQ=255)
                                                                             aaagaaaCGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGAGTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCa                                                                  >  2:2149471/1‑148 (MQ=255)
                                                                             aaagaaaCGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCa                                                                  >  2:2420059/1‑148 (MQ=255)
                                                                             aaagaaaCGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCa                                                                  >  2:658166/1‑148 (MQ=255)
                                                                              aagaaaCGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCat                                                                 <  1:3186352/148‑1 (MQ=255)
                                                                              aagaaaCGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCat                                                                 <  2:765766/148‑1 (MQ=255)
                                                                              aagaaaCGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCat                                                                 <  1:3390792/148‑1 (MQ=255)
                                                                              aagaaaCGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCat                                                                 <  2:1264274/148‑1 (MQ=255)
                                                                               agaaaCGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCata                                                                >  2:2472904/1‑148 (MQ=255)
                                                                               agaaaCGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCata                                                                >  1:712303/1‑148 (MQ=255)
                                                                               agaaaCGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCata                                                                >  2:178431/1‑148 (MQ=255)
                                                                                 aaaCGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGNAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATatc                                                              <  1:1780842/148‑1 (MQ=255)
                                                                                 aaaCGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATatc                                                              >  2:2220028/1‑148 (MQ=255)
                                                                                  aaCGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATatca                                                             <  1:583791/148‑1 (MQ=255)
                                                                                  aaCGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATatca                                                             <  1:2346706/148‑1 (MQ=255)
                                                                                   aCGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATatcat                                                            >  1:3027125/1‑148 (MQ=255)
                                                                                    agatGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCTTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATatcatc                                                           <  2:1791188/145‑1 (MQ=255)
                                                                                       tGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCata                                                        <  2:493263/148‑1 (MQ=255)
                                                                                        ttaaaatGGCAAAAAGCCCAGCGAAAAATAATTCAATTTGGTGTGCTGTCCGGATTTTTTTTAGGCGTATTGCCCGTTTTTTCGTATTTGTGTCAATGTTTTTTTTAATTAAACTTCCTTGTATCCAAAATAGTGTCATATCATCata                                                        <  1:1391272/146‑1 (MQ=255)
                                                                                        gCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCatat                                                       <  2:2558601/148‑1 (MQ=255)
                                                                                         cAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATAtt                                                      >  2:2147520/1‑148 (MQ=255)
                                                                                           aaaaGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTaa                                                    <  1:3067300/148‑1 (MQ=255)
                                                                                            aaaGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAAt                                                   >  2:1919941/1‑148 (MQ=255)
                                                                                            aaaGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAAt                                                   <  1:972615/148‑1 (MQ=255)
                                                                                              aGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAATTg                                                 <  1:2887658/148‑1 (MQ=255)
                                                                                               ggCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAATTGt                                                <  2:1189038/148‑1 (MQ=255)
                                                                                               ggCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTAGGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAATTGt                                                <  1:2747058/148‑1 (MQ=255)
                                                                                                gCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAATTGtt                                               >  1:2027063/1‑148 (MQ=255)
                                                                                                gCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAATTGtt                                               <  2:2454221/148‑1 (MQ=255)
                                                                                                 cAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAATTGTTc                                              <  2:2129292/148‑1 (MQ=255)
                                                                                                 cAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAATTGTTc                                              <  2:1704924/148‑1 (MQ=255)
                                                                                                 cAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAATTGTTc                                              >  1:2886094/1‑148 (MQ=255)
                                                                                                 cAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCAGATCATCATATTAAGTGTTc                                              >  2:579439/1‑148 (MQ=255)
                                                                                                  aaaaCGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAATTGTTCt                                             <  1:998038/148‑1 (MQ=255)
                                                                                                     aCGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAATTGTTCtttt                                          <  1:3204957/148‑1 (MQ=255)
                                                                                                      cGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAATTGTTCttttt                                         >  1:2926627/1‑148 (MQ=255)
                                                                                                            gCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGt                                   >  1:2853545/1‑148 (MQ=255)
                                                                                                            gCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGt                                   >  1:231492/1‑148 (MQ=255)
                                                                                                              gAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGa                                 <  2:386433/148‑1 (MQ=255)
                                                                                                               aaaaaTAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGaa                                >  2:25878/1‑148 (MQ=255)
                                                                                                               aaaaaTAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGaa                                >  1:3142825/1‑148 (MQ=255)
                                                                                                                   ataatGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGtt                            <  1:988054/148‑1 (MQ=255)
                                                                                                                    taatGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTc                           >  1:2310335/1‑148 (MQ=255)
                                                                                                                       tGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCa                        >  1:493923/1‑148 (MQ=255)
                                                                                                                       tGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCa                        >  2:1320696/1‑148 (MQ=255)
                                                                                                                       tGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCa                        >  2:1320684/1‑148 (MQ=255)
                                                                                                                             atCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTc                  >  1:3483317/1‑148 (MQ=255)
                                                                                                                               cGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTg                <  1:1032336/148‑1 (MQ=255)
                                                                                                                                 ggTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGa              <  1:1102442/148‑1 (MQ=255)
                                                                                                                                 ggTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGa              <  1:186297/148‑1 (MQ=255)
                                                                                                                                      tGACCGGATATTTTTCCGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AACTAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCt         <  2:2277980/148‑1 (MQ=255)
                                                                                                                                            gATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGTTGAAGGTTCCCATGCGTCTGGAAGTCTTTtgtg   >  2:2314845/1‑148 (MQ=255)
                                                                                                                                            gATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTtgtg   >  2:3513057/1‑148 (MQ=255)
                                                                                                                                             atatTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGa  <  1:3333443/148‑1 (MQ=255)
                                                                                                                                             atatTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGa  <  1:3268091/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCC‑AAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGA  >  CP009273/1380711‑1380999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAG  >  CP009273/1380707‑1381001
                                                                                                                                                     |                                                                                                                                                 
GAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATT                                                                                                                                                   <  SRR19973065.1148073/150‑1 (MQ=60)
 AGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAACACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTT                                                                                                                                                  <  SRR19973065.740502/150‑1 (MQ=60)
 AGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTT                                                                                                                                                  >  SRR19973065.637653/1‑150 (MQ=60)
   CTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGTGATTTCCGTCGTCAGCTTATCGGTCAGGTTAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTT                                                                                                                                                <  SRR19973065.383267/150‑1 (MQ=60)
   CTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTT                                                                                                                                                <  SRR19973065.1231968/150‑1 (MQ=60)
   CTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTT                                                                                                                                                <  SRR19973065.1231984/150‑1 (MQ=60)
   CTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTT                                                                                                                                                <  SRR19973065.1232616/150‑1 (MQ=60)
               CTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGC                                                                                                                                    >  SRR19973065.2292351/1‑150 (MQ=60)
                    GTGGATTGACGATGACAAACCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTT                                                                                                                               <  SRR19973065.2399330/150‑1 (MQ=60)
                     TGGATTGACGATGACAAACCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTT                                                                                                                              >  SRR19973065.2651973/1‑150 (MQ=60)
                             CGATGACAAACCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCT                                                                                                                      <  SRR19973065.805342/150‑1 (MQ=60)
                              GATGACAAACCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTT                                                                                                                     <  SRR19973065.316498/150‑1 (MQ=60)
                                TGACAAACCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTG                                                                                                                   >  SRR19973065.1448197/1‑150 (MQ=60)
                                   CAAACCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGT                                                                                                                >  SRR19973065.1272607/1‑150 (MQ=60)
                                   CAAACCTCGCCTCGGGGATGTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGT                                                                                                                <  SRR19973065.408802/150‑1 (MQ=60)
                                    AAACCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTC                                                                                                               >  SRR19973065.2842449/1‑150 (MQ=60)
                                          CGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGTTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGAT                                                                                                         <  SRR19973065.147158/150‑1 (MQ=60)
                                          CGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGAT                                                                                                         <  SRR19973065.147124/150‑1 (MQ=60)
                                          CGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGAT                                                                                                         <  SRR19973065.3340711/150‑1 (MQ=60)
                                            CCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTG                                                                                                       >  SRR19973065.1527140/1‑150 (MQ=60)
                                              TCGGGGATTTCCGTCGTCAGGTTATCGGTCATTTTTAAGAAACGCTTCAAAAAGGCAACACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTT                                                                                                     <  SRR19973065.1526759/150‑1 (MQ=60)
                                              TCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTT                                                                                                     <  SRR19973065.61236/150‑1 (MQ=60)
                                              TCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTT                                                                                                     <  SRR19973065.1849506/150‑1 (MQ=60)
                                               CGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTG                                                                                                    <  SRR19973065.3014423/150‑1 (MQ=60)
                                               CGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTG                                                                                                    <  SRR19973065.1259633/150‑1 (MQ=60)
                                                   GATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAG                                                                                                >  SRR19973065.2574600/1‑150 (MQ=60)
                                                     TTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAA                                                                                              <  SRR19973065.2918161/150‑1 (MQ=60)
                                                     TATCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAA                                                                                              >  SRR19973065.169758/1‑150 (MQ=60)
                                                      TTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAA                                                                                             >  SRR19973065.3329585/1‑150 (MQ=60)
                                                         CGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCT                                                                                          >  SRR19973065.807379/1‑150 (MQ=60)
                                                          GTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTT                                                                                         >  SRR19973065.2129714/1‑150 (MQ=60)
                                                          GTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTT                                                                                         >  SRR19973065.2129739/1‑150 (MQ=60)
                                                            CGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCC                                                                                       >  SRR19973065.1508715/1‑150 (MQ=60)
                                                             GTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCT                                                                                      <  SRR19973065.3425045/150‑1 (MQ=60)
                                                             GTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCT                                                                                      >  SRR19973065.595039/1‑150 (MQ=60)
                                                              TCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTG                                                                                     <  SRR19973065.599492/150‑1 (MQ=60)
                                                                AGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCT                                                                                   <  SRR19973065.1366354/150‑1 (MQ=60)
                                                                AGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCT                                                                                   <  SRR19973065.1366101/150‑1 (MQ=60)
                                                                  CTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTAT                                                                                 >  SRR19973065.3074748/1‑150 (MQ=60)
                                                                   TTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATC                                                                                >  SRR19973065.3055844/1‑150 (MQ=60)
                                                                   TTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATC                                                                                >  SRR19973065.3055836/1‑150 (MQ=60)
                                                                       CGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAA                                                                            >  SRR19973065.2112111/1‑150 (MQ=60)
                                                                        GGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAAT                                                                           >  SRR19973065.702925/1‑150 (MQ=60)
                                                                        GGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAAT                                                                           <  SRR19973065.1884762/150‑1 (MQ=60)
                                                                           CAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGT                                                                        >  SRR19973065.1511614/1‑150 (MQ=60)
                                                                            AGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTG                                                                       >  SRR19973065.496584/1‑150 (MQ=60)
                                                                            AGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTG                                                                       >  SRR19973065.814466/1‑150 (MQ=60)
                                                                                GAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCAT                                                                   <  SRR19973065.2426364/150‑1 (MQ=60)
                                                                                GAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCAT                                                                   <  SRR19973065.752446/150‑1 (MQ=60)
                                                                                 AAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATA                                                                  >  SRR19973065.717070/1‑150 (MQ=60)
                                                                                  AAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATAT                                                                 <  SRR19973065.3205586/150‑1 (MQ=60)
                                                                                  AAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATAT                                                                 <  SRR19973065.3411535/150‑1 (MQ=60)
                                                                                     AAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGNAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCAT                                                              <  SRR19973065.1792470/150‑1 (MQ=60)
                                                                                     AAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCAT                                                              >  SRR19973065.3045675/1‑150 (MQ=60)
                                                                                      AACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATC                                                             <  SRR19973065.2361338/150‑1 (MQ=60)
                                                                                      AACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATC                                                             <  SRR19973065.3243432/150‑1 (MQ=60)
                                                                                      AACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATC                                                             <  SRR19973065.587425/150‑1 (MQ=60)
                                                                                      AACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATC                                                             <  SRR19973065.587293/150‑1 (MQ=60)
                                                                                           TGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATT                                                        <  SRR19973065.3155381/150‑1 (MQ=60)
                                                                                              AAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAAT                                                     >  SRR19973065.2484646/1‑150 (MQ=60)
                                                                                               AAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATT                                                    <  SRR19973065.3086052/150‑1 (MQ=60)
                                                                                               AAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATT                                                    >  SRR19973065.94875/1‑150 (MQ=60)
                                                                                                AAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTG                                                   <  SRR19973065.979071/150‑1 (MQ=60)
                                                                                                AAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATGAATTG                                                   >  SRR19973065.2232882/1‑150 (MQ=60)
                                                                                                  AGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTT                                                 >  SRR19973065.2040695/1‑150 (MQ=60)
                                                                                                  AGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTT                                                 >  SRR19973065.3325991/1‑150 (MQ=60)
                                                                                                  AGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTT                                                 >  SRR19973065.2040087/1‑150 (MQ=60)
                                                                                                  AGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTT                                                 <  SRR19973065.2905314/150‑1 (MQ=60)
                                                                                                   GGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTC                                                >  SRR19973065.2903744/1‑150 (MQ=60)
                                                                                                   GGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTAGGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTC                                                <  SRR19973065.2764153/150‑1 (MQ=60)
                                                                                                      AAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTT                                             <  SRR19973065.1004668/150‑1 (MQ=60)
                                                                                                      AAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTT                                             <  SRR19973065.1004664/150‑1 (MQ=60)
                                                                                                       AAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTT                                            <  SRR19973065.3405092/150‑1 (MQ=60)
                                                                                                        AACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTT                                           >  SRR19973065.2944402/1‑150 (MQ=60)
                                                                                                         ACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTT                                          <  SRR19973065.3224249/150‑1 (MQ=60)
                                                                                                          CGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTC                                         <  SRR19973065.1749289/150‑1 (MQ=60)
                                                                                                              CAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGT                                     >  SRR19973065.2871232/1‑150 (MQ=60)
                                                                                                              CAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGT                                     >  SRR19973065.2871087/1‑150 (MQ=60)
                                                                                                              CAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGT                                     >  SRR19973065.233176/1‑150 (MQ=60)
                                                                                                                 CGAAAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAA                                  >  SRR19973065.3161921/1‑150 (MQ=60)
                                                                                                                    AAAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGT                               >  SRR19973065.2356817/1‑150 (MQ=60)
                                                                                                                     AAATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTT                              <  SRR19973065.3508092/150‑1 (MQ=60)
                                                                                                                      AATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTC                             >  SRR19973065.2324845/1‑150 (MQ=60)
                                                                                                                      AATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTC                             >  SRR19973065.2324971/1‑150 (MQ=60)
                                                                                                                       ATAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCC                            <  SRR19973065.994615/150‑1 (MQ=60)
                                                                                                                        TAATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCC                           <  SRR19973065.711656/150‑1 (MQ=60)
                                                                                                                         AATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCA                          >  SRR19973065.497246/1‑150 (MQ=60)
                                                                                                                          ATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCAT                         >  SRR19973065.472217/1‑150 (MQ=60)
                                                                                                                          ATGCAATATCGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCAT                         >  SRR19973065.1655467/1‑150 (MQ=60)
                                                                                                                               ATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTC                    >  SRR19973065.3504510/1‑150 (MQ=60)
                                                                                                                                   CGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGA                >  SRR19973065.1159674/1‑150 (MQ=60)
                                                                                                                                   CGGGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGA                <  SRR19973065.1039168/150‑1 (MQ=60)
                                                                                                                                     GGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAG              <  SRR19973065.187758/150‑1 (MQ=60)
                                                                                                                                     GGTGCTGACCGGATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAG              <  SRR19973065.1109682/150‑1 (MQ=60)
                                                                                                                                                 ATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAG  <  SRR19973065.3287909/150‑1 (MQ=60)
                                                                                                                                                 ATATTTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAG  <  SRR19973065.3353797/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                 
GAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAG  >  CP009273/1380707‑1381001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑