Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,850,790 C→A 100% I113I (ATC→ATA)  mutS → methyl‑directed mismatch repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,850,7900CA99.0% 708.8 / ‑1.8 221I113I (ATC→ATA) mutSmethyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (110/108);  total (112/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 223 total aligned reads displayed.

GATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATCGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCT  >  CP009273/2850646‑2850934
                                                                                                                                                |                                                                                                                                                
gATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGtt                                                                                                                                               <  2:2284319/148‑1 (MQ=255)
 aTCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCACAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTa                                                                                                                                              >  2:2481486/1‑148 (MQ=255)
 aTCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTa                                                                                                                                              <  1:1423707/148‑1 (MQ=255)
  tCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTAc                                                                                                                                             >  1:3084853/1‑148 (MQ=255)
   cccGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACg                                                                                                                                            >  1:3136929/1‑148 (MQ=255)
    ccGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGc                                                                                                                                           <  1:2337143/148‑1 (MQ=255)
     cGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGcc                                                                                                                                          >  2:2836628/1‑148 (MQ=255)
     cGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGcc                                                                                                                                          >  1:887205/1‑148 (MQ=255)
              ggATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAANAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCAt                                                                                                                                 <  1:1782932/148‑1 (MQ=255)
               gATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATCGTTACGCCAGGCACCATc                                                                                                                                >  1:354808/1‑148 (MQ=255)
               gATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATc                                                                                                                                <  2:3018490/148‑1 (MQ=255)
                 ttCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAg                                                                                                                              >  1:1354947/1‑148 (MQ=255)
                 ttCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAg                                                                                                                              <  2:1407004/148‑1 (MQ=255)
                 ttCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAg                                                                                                                              >  1:764880/1‑148 (MQ=255)
                  tCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGc                                                                                                                             >  1:2058155/1‑148 (MQ=255)
                   cccTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCg                                                                                                                            <  2:303774/148‑1 (MQ=255)
                     cTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGAt                                                                                                                          >  2:617096/1‑148 (MQ=255)
                     cTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGAt                                                                                                                          >  1:882362/1‑148 (MQ=255)
                      tACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATg                                                                                                                         >  1:1312954/1‑148 (MQ=255)
                      tACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATg                                                                                                                         <  1:860379/148‑1 (MQ=255)
                      tACCATGCGGTGGAAAAATATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATg                                                                                                                         <  1:2709959/148‑1 (MQ=255)
                      tACCATGCGGTGGAAAAATATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATg                                                                                                                         <  1:2710198/148‑1 (MQ=255)
                      tACCATGCGGTGGAAAAATATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATg                                                                                                                         <  1:2310155/148‑1 (MQ=255)
                        ccATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGaa                                                                                                                       >  2:1897390/1‑148 (MQ=255)
                        ccATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGaa                                                                                                                       <  1:2892899/148‑1 (MQ=255)
                          aTGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGc                                                                                                                     <  2:1019499/148‑1 (MQ=255)
                           tGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGcc                                                                                                                    >  2:749750/1‑148 (MQ=255)
                              ggTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTg                                                                                                                 <  2:912902/148‑1 (MQ=255)
                                     aaCTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAgg                                                                                                          <  2:1419916/148‑1 (MQ=255)
                                      aCTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGa                                                                                                         >  2:25298/1‑148 (MQ=255)
                                          tctcGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGt                                                                                                     >  2:245452/1‑148 (MQ=255)
                                                  aaCTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACaa                                                                                             <  2:307506/148‑1 (MQ=255)
                                                  aaCTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACaa                                                                                             <  1:3281875/148‑1 (MQ=255)
                                                  aaCTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACaa                                                                                             <  2:3348292/148‑1 (MQ=255)
                                                   aCTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAAc                                                                                            >  2:77432/1‑148 (MQ=255)
                                                   aCTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAAc                                                                                            >  2:1391280/1‑148 (MQ=255)
                                                   aCTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAAc                                                                                            <  1:297155/148‑1 (MQ=255)
                                                    cTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCAAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAAcc                                                                                           >  2:1209009/1‑148 (MQ=255)
                                                      ggTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACctg                                                                                         <  1:2329750/148‑1 (MQ=255)
                                                       gTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACctgc                                                                                        <  2:2119815/148‑1 (MQ=255)
                                                          aaTCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTgg                                                                                     >  1:3424970/1‑148 (MQ=255)
                                                          aaTCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTgg                                                                                     >  1:365678/1‑148 (MQ=255)
                                                          aaTCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTgg                                                                                     >  2:1488932/1‑148 (MQ=255)
                                                            tCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTggcg                                                                                   >  2:2920879/1‑148 (MQ=255)
                                                            tCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTggcg                                                                                   >  2:1169938/1‑148 (MQ=255)
                                                             cAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCACAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTggcgg                                                                                  >  2:1896308/1‑148 (MQ=255)
                                                                  agagTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATc                                                                             <  1:549101/148‑1 (MQ=255)
                                                                     gTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTgg                                                                          <  2:258663/148‑1 (MQ=255)
                                                                      tCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGc                                                                         >  1:60509/1‑148 (MQ=255)
                                                                          ttGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAAGa                                                                     >  2:2099479/1‑148 (MQ=255)
                                                                           tGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGAc                                                                    >  2:1946445/1‑148 (MQ=255)
                                                                           tGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGAc                                                                    <  1:1208761/148‑1 (MQ=255)
                                                                             ccATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAg                                                                  >  1:1619575/1‑148 (MQ=255)
                                                                               aTCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCa                                                                <  2:1911297/148‑1 (MQ=255)
                                                                                tCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCaa                                                               <  2:2831000/148‑1 (MQ=255)
                                                                                 cTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATCGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCaaa                                                              <  2:354808/148‑1 (MQ=255)
                                                                                 cTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCaaa                                                              <  2:2607508/148‑1 (MQ=255)
                                                                                   gCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAgg                                                            <  1:1291696/148‑1 (MQ=255)
                                                                                    cGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGt                                                           >  2:3441599/1‑148 (MQ=255)
                                                                                    cGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGt                                                           <  2:44435/148‑1 (MQ=255)
                                                                                      aaCAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGttt                                                         <  1:618160/148‑1 (MQ=255)
                                                                                       aCAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTc                                                        >  1:1473422/1‑148 (MQ=255)
                                                                                          aaTTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGc                                                     >  2:1747600/1‑148 (MQ=255)
                                                                                           aTTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCt                                                    <  2:3165781/148‑1 (MQ=255)
                                                                                           aTTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCt                                                    >  1:1028729/1‑148 (MQ=255)
                                                                                               gCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTAcgc                                                >  1:853183/1‑148 (MQ=255)
                                                                                                 gATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGa                                              <  1:962066/148‑1 (MQ=255)
                                                                                                 gATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGa                                              <  2:1914825/148‑1 (MQ=255)
                                                                                                  aTCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGAc                                             >  1:3093497/1‑148 (MQ=255)
                                                                                                   tCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACg                                            >  2:563582/1‑148 (MQ=255)
                                                                                                   tCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTAGAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACg                                            >  2:2228540/1‑148 (MQ=255)
                                                                                                          aCCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGata                                     >  2:1426386/1‑148 (MQ=255)
                                                                                                              gCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAg                                 <  1:1946445/148‑1 (MQ=255)
                                                                                                              gCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAg                                 >  1:187345/1‑148 (MQ=255)
                                                                                                               cAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGt                                >  2:2224470/1‑148 (MQ=255)
                                                                                                                aaaGGTCCGGTTGAGCTCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGtt                               <  2:814325/148‑1 (MQ=255)
                                                                                                                aaaGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGtt                               <  2:887205/148‑1 (MQ=255)
                                                                                                                 aaGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTc                              >  2:2292229/1‑148 (MQ=255)
                                                                                                                 aaGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTc                              >  1:952862/1‑148 (MQ=255)
                                                                                                                  aGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTcc                             >  1:2174400/1‑148 (MQ=255)
                                                                                                                  aGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTcc                             >  1:1080393/1‑148 (MQ=255)
                                                                                                                   ggTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCg                            >  2:268188/1‑148 (MQ=255)
                                                                                                                    gTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCgg                           >  1:2802079/1‑148 (MQ=255)
                                                                                                                    gTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCgg                           >  1:1050549/1‑148 (MQ=255)
                                                                                                                          ttGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGttt                     <  2:1602033/148‑1 (MQ=255)
                                                                                                                           tGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGtttt                    <  2:1869061/148‑1 (MQ=255)
                                                                                                                              gcgcAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGc                 <  1:245579/148‑1 (MQ=255)
                                                                                                                               cgcAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGcc                <  1:1591164/148‑1 (MQ=255)
                                                                                                                               cgcAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGcc                <  1:1590544/148‑1 (MQ=255)
                                                                                                                                 cAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTg              >  2:388251/1‑148 (MQ=255)
                                                                                                                                    aGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTAGCCTGAGc           >  1:2374407/1‑148 (MQ=255)
                                                                                                                                      ttGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATAGCAGTTCCGGGCGTGTTCGCCTGAGCGa         >  1:2075534/1‑148 (MQ=255)
                                                                                                                                       tgtgCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGaa        >  1:2611528/1‑148 (MQ=255)
                                                                                                                                        gtgCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAAc       <  2:2736861/148‑1 (MQ=255)
                                                                                                                                        gtgCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAAc       <  2:2065035/148‑1 (MQ=255)
                                                                                                                                           cGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCgg    <  1:1391280/148‑1 (MQ=255)
                                                                                                                                           cGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCgg    <  2:1355038/148‑1 (MQ=255)
                                                                                                                                            gTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGc   <  1:2066468/148‑1 (MQ=255)
                                                                                                                                            gTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGc   <  1:1461655/148‑1 (MQ=255)
                                                                                                                                             tataGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCt  <  1:1860521/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATCGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCT  >  CP009273/2850646‑2850934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAGCCGATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATCGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGAC  >  CP009273/2850641‑2850937
                                                                                                                                                     |                                                                                                                                                   
GAGCCGATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATA                                                                                                                                                     >  SRR19973065.1970041/1‑150 (MQ=60)
GAGCCGATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATA                                                                                                                                                     <  SRR19973065.983165/150‑1 (MQ=60)
GAGCCGATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATA                                                                                                                                                     <  SRR19973065.505070/150‑1 (MQ=60)
GAGCCGATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATA                                                                                                                                                     <  SRR19973065.2085026/150‑1 (MQ=60)
GAGCCGATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATA                                                                                                                                                     <  SRR19973065.495921/150‑1 (MQ=60)
GAGCCGATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATA                                                                                                                                                     >  SRR19973065.1429183/1‑150 (MQ=60)
GAGCCGATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATA                                                                                                                                                     >  SRR19973065.1429176/1‑150 (MQ=60)
 AGCCGATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAG                                                                                                                                                    <  SRR19973065.1970275/150‑1 (MQ=60)
  GCCGATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGT                                                                                                                                                   <  SRR19973065.2553861/150‑1 (MQ=60)
     GATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTAC                                                                                                                                                >  SRR19973065.3103689/1‑150 (MQ=60)
      ATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACG                                                                                                                                               <  SRR19973065.1432986/150‑1 (MQ=60)
      ATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACG                                                                                                                                               >  SRR19973065.3156008/1‑150 (MQ=60)
      ATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACG                                                                                                                                               >  SRR19973065.3156001/1‑150 (MQ=60)
        CCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCC                                                                                                                                             >  SRR19973065.892841/1‑150 (MQ=60)
         CCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCA                                                                                                                                            <  SRR19973065.2351734/150‑1 (MQ=60)
           GATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGG                                                                                                                                          <  SRR19973065.3531839/150‑1 (MQ=60)
           GATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGG                                                                                                                                          <  SRR19973065.1306476/150‑1 (MQ=60)
           GATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGG                                                                                                                                          <  SRR19973065.1306422/150‑1 (MQ=60)
             TGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCA                                                                                                                                        <  SRR19973065.2522810/150‑1 (MQ=60)
                  GGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATCGTTACGCCAGGCACCATC                                                                                                                                   >  SRR19973065.357305/1‑150 (MQ=60)
                   GGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAANAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCA                                                                                                                                  <  SRR19973065.1794588/150‑1 (MQ=60)
                    GATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAG                                                                                                                                 >  SRR19973065.1363926/1‑150 (MQ=60)
                    GATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAG                                                                                                                                 >  SRR19973065.1364018/1‑150 (MQ=60)
                    GATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAG                                                                                                                                 >  SRR19973065.769966/1‑150 (MQ=60)
                     ATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGC                                                                                                                                >  SRR19973065.2071293/1‑150 (MQ=60)
                     ATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGC                                                                                                                                >  SRR19973065.2078196/1‑150 (MQ=60)
                        CCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGAT                                                                                                                             >  SRR19973065.887980/1‑150 (MQ=60)
                        CCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGAT                                                                                                                             >  SRR19973065.887962/1‑150 (MQ=60)
                         CCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATG                                                                                                                            >  SRR19973065.1321634/1‑150 (MQ=60)
                           TACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAA                                                                                                                          <  SRR19973065.865494/150‑1 (MQ=60)
                           TACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAA                                                                                                                          <  SRR19973065.865921/150‑1 (MQ=60)
                           TACCATGCGGTGGAAAAATATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAA                                                                                                                          <  SRR19973065.2726816/150‑1 (MQ=60)
                           TACCATGCGGTGGAAAAATATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAA                                                                                                                          <  SRR19973065.2727048/150‑1 (MQ=60)
                           TACCATGCGGTGGAAAAATATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAA                                                                                                                          <  SRR19973065.2726809/150‑1 (MQ=60)
                           TACCATGCGGTGGAAAAATATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAA                                                                                                                          <  SRR19973065.2324665/150‑1 (MQ=60)
                             CCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGC                                                                                                                        <  SRR19973065.2910569/150‑1 (MQ=60)
                                    GTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTG                                                                                                                 <  SRR19973065.2609343/150‑1 (MQ=60)
                                     TGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGC                                                                                                                >  SRR19973065.3131284/1‑150 (MQ=60)
                                     TGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGC                                                                                                                >  SRR19973065.2081517/1‑150 (MQ=60)
                                        AAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGG                                                                                                             >  SRR19973065.905497/1‑150 (MQ=60)
                                               TCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCA                                                                                                      <  SRR19973065.1467423/150‑1 (MQ=60)
                                               TCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCA                                                                                                      <  SRR19973065.1467420/150‑1 (MQ=60)
                                                CTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAG                                                                                                     >  SRR19973065.994218/1‑150 (MQ=60)
                                                      AAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAAC                                                                                               <  SRR19973065.3443340/150‑1 (MQ=60)
                                                      AAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAAC                                                                                               <  SRR19973065.1666802/150‑1 (MQ=60)
                                                       AACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACC                                                                                              <  SRR19973065.3301826/150‑1 (MQ=60)
                                                        ACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCT                                                                                             <  SRR19973065.299073/150‑1 (MQ=60)
                                                        ACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCT                                                                                             >  SRR19973065.2853028/1‑150 (MQ=60)
                                                        ACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCT                                                                                             <  SRR19973065.2199045/150‑1 (MQ=60)
                                                         CTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTG                                                                                            >  SRR19973065.2019551/1‑150 (MQ=60)
                                                           GGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCT                                                                                          <  SRR19973065.2344324/150‑1 (MQ=60)
                                                             TGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGG                                                                                        >  SRR19973065.3445875/1‑150 (MQ=60)
                                                             TGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGG                                                                                        >  SRR19973065.368240/1‑150 (MQ=60)
                                                             TGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGG                                                                                        >  SRR19973065.367836/1‑150 (MQ=60)
                                                                      GAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCT                                                                               <  SRR19973065.518014/150‑1 (MQ=60)
                                                                      GAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCT                                                                               <  SRR19973065.1988127/150‑1 (MQ=60)
                                                                      GAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCT                                                                               >  SRR19973065.1083430/1‑150 (MQ=60)
                                                                      GAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCT                                                                               <  SRR19973065.518072/150‑1 (MQ=60)
                                                                       AGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTG                                                                              >  SRR19973065.1661956/1‑150 (MQ=60)
                                                                       AGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTG                                                                              <  SRR19973065.2363980/150‑1 (MQ=60)
                                                                       AGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTG                                                                              <  SRR19973065.552636/150‑1 (MQ=60)
                                                                       AGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTG                                                                              >  SRR19973065.888431/1‑150 (MQ=60)
                                                                         AGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACTCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGC                                                                            >  SRR19973065.61217/1‑150 (MQ=60)
                                                                         AGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGC                                                                            >  SRR19973065.61150/1‑150 (MQ=60)
                                                                              GTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGAC                                                                       <  SRR19973065.1185842/150‑1 (MQ=60)
                                                                              GTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGAC                                                                       <  SRR19973065.742796/150‑1 (MQ=60)
                                                                                TGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAG                                                                     >  SRR19973065.1630435/1‑150 (MQ=60)
                                                                                TGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAG                                                                     <  SRR19973065.1216549/150‑1 (MQ=60)
                                                                                TGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAG                                                                     <  SRR19973065.1216801/150‑1 (MQ=60)
                                                                                  CCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCA                                                                   >  SRR19973065.1608644/1‑150 (MQ=60)
                                                                                        GCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTT                                                             <  SRR19973065.1300207/150‑1 (MQ=60)
                                                                                         CGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTAGAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTCTCTGGCAGGACAGCACAGGTTT                                                            >  SRR19973065.2856408/1‑150 (MQ=60)
                                                                                          GAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTC                                                           >  SRR19973065.1482772/1‑150 (MQ=60)
                                                                                          GAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTC                                                           >  SRR19973065.1482854/1‑150 (MQ=60)
                                                                                           AACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCG                                                          <  SRR19973065.622131/150‑1 (MQ=60)
                                                                                           AACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCG                                                          <  SRR19973065.1929778/150‑1 (MQ=60)
                                                                                             CAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGC                                                        >  SRR19973065.2328480/1‑150 (MQ=60)
                                                                                              ACATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCT                                                       >  SRR19973065.1036385/1‑150 (MQ=60)
                                                                                              AAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCT                                                       >  SRR19973065.1035541/1‑150 (MQ=60)
                                                                                                ATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTAC                                                     >  SRR19973065.930562/1‑150 (MQ=60)
                                                                                                  TGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGC                                                   >  SRR19973065.858704/1‑150 (MQ=60)
                                                                                                     CGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGAC                                                >  SRR19973065.3112178/1‑150 (MQ=60)
                                                                                                     CGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGAC                                                >  SRR19973065.3112385/1‑150 (MQ=60)
                                                                                                      GATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACG                                               <  SRR19973065.968450/150‑1 (MQ=60)
                                                                                                      GATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACG                                               >  SRR19973065.3026669/1‑150 (MQ=60)
                                                                                                      GATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACG                                               <  SRR19973065.2733649/150‑1 (MQ=60)
                                                                                                      GATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACG                                               <  SRR19973065.1492782/150‑1 (MQ=60)
                                                                                                      GATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACG                                               >  SRR19973065.571520/1‑150 (MQ=60)
                                                                                                      GATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACG                                               >  SRR19973065.2346984/1‑150 (MQ=60)
                                                                                                         CCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTG                                            >  SRR19973065.1330812/1‑150 (MQ=60)
                                                                                                             CGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATA                                        <  SRR19973065.326836/150‑1 (MQ=60)
                                                                                                              GACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATAT                                       <  SRR19973065.2287244/150‑1 (MQ=60)
                                                                                                                 CAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAG                                    >  SRR19973065.188814/1‑150 (MQ=60)
                                                                                                                 CAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAG                                    >  SRR19973065.188799/1‑150 (MQ=60)
                                                                                                                   GCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTT                                  <  SRR19973065.1959168/150‑1 (MQ=60)
                                                                                                                    CAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTC                                 >  SRR19973065.959159/1‑150 (MQ=60)
                                                                                                                    CAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTC                                 >  SRR19973065.959162/1‑150 (MQ=60)
                                                                                                                     AAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCC                                >  SRR19973065.2187025/1‑150 (MQ=60)
                                                                                                                     AAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCC                                >  SRR19973065.1087523/1‑150 (MQ=60)
                                                                                                                     AAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCC                                >  SRR19973065.2188351/1‑150 (MQ=60)
                                                                                                                       AGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGG                              >  SRR19973065.2819432/1‑150 (MQ=60)
                                                                                                                       AGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGG                              >  SRR19973065.1057505/1‑150 (MQ=60)
                                                                                                                             GGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTT                        >  SRR19973065.2971432/1‑150 (MQ=60)
                                                                                                                                   GCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCT                  <  SRR19973065.247192/150‑1 (MQ=60)
                                                                                                                                   GCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCT                  <  SRR19973065.247319/150‑1 (MQ=60)
                                                                                                                                    CGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTG                 <  SRR19973065.1601789/150‑1 (MQ=60)
                                                                                                                                    CGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTG                 <  SRR19973065.1601255/150‑1 (MQ=60)
                                                                                                                                    CGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTG                 <  SRR19973065.1601164/150‑1 (MQ=60)
                                                                                                                                       AAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTAGCCTGAGC              >  SRR19973065.2389229/1‑150 (MQ=60)
                                                                                                                                         AGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATAGCAGTTCCGGGCGTGTTCGCCTGAGCGA            >  SRR19973065.2088798/1‑150 (MQ=60)
                                                                                                                                          GTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAACGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAA           >  SRR19973065.2627836/1‑150 (MQ=60)
                                                                                                                                          GTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAA           >  SRR19973065.2627719/1‑150 (MQ=60)
                                                                                                                                                CGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCT     <  SRR19973065.78334/150‑1 (MQ=60)
                                                                                                                                                CGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCT     <  SRR19973065.1400417/150‑1 (MQ=60)
                                                                                                                                                CGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCT     <  SRR19973065.1400413/150‑1 (MQ=60)
                                                                                                                                                CGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCT     <  SRR19973065.78216/150‑1 (MQ=60)
                                                                                                                                                CGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCT     <  SRR19973065.1867168/150‑1 (MQ=60)
                                                                                                                                                 GTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTG    <  SRR19973065.1471048/150‑1 (MQ=60)
                                                                                                                                                 GTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTG    <  SRR19973065.2079635/150‑1 (MQ=60)
                                                                                                                                                  TATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGA   <  SRR19973065.1872765/150‑1 (MQ=60)
                                                                                                                                                   ATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGTTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGAC  <  SRR19973065.1044713/150‑1 (MQ=60)
                                                                                                                                                   ATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGTTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGAC  <  SRR19973065.2807482/150‑1 (MQ=60)
                                                                                                                                                   ATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGTTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGAC  <  SRR19973065.1044687/150‑1 (MQ=60)
                                                                                                                                                   ATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGAC  >  SRR19973065.2934400/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
GAGCCGATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATCGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGAC  >  CP009273/2850641‑2850937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑