Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,850,790 C→A 100% I113I (ATC→ATA)  mutS → methyl‑directed mismatch repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,850,7900CA99.6% 865.6 / ‑4.5 268I113I (ATC→ATA) mutSmethyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (144/123);  total (145/123)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 268 total aligned reads displayed.

CCGATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATCGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTG  >  CP009273/2850644‑2850935
                                                                                                                                                  |                                                                                                                                                 
 cGATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTa                                                                                                                                               >  2:3061384/1‑150 (MQ=255)
 cGATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTa                                                                                                                                               >  2:2775923/1‑150 (MQ=255)
  gATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTTTAGTTAc                                                                                                                                              <  1:5217095/150‑1 (MQ=255)
  gATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTAc                                                                                                                                              >  1:1286334/1‑150 (MQ=255)
    tCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGc                                                                                                                                            >  1:5161585/1‑150 (MQ=255)
     cccGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGcc                                                                                                                                           <  1:2456235/150‑1 (MQ=255)
       cGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCAGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAg                                                                                                                                         >  2:3813421/1‑150 (MQ=255)
           ggCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCAc                                                                                                                                     >  2:2336602/1‑150 (MQ=255)
            gCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCTCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGTTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCAcc                                                                                                                                    <  2:250586/150‑1 (MQ=255)
              ggggATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCAt                                                                                                                                  >  1:4212065/1‑150 (MQ=255)
               gggATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTCGAGTTCCGCCCGGCACCATc                                                                                                                                 >  2:4188810/1‑150 (MQ=255)
               gggATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATc                                                                                                                                 >  2:4188821/1‑150 (MQ=255)
                ggATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCa                                                                                                                                <  1:414648/150‑1 (MQ=255)
                ggATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCa                                                                                                                                <  2:188619/150‑1 (MQ=255)
                ggATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCa                                                                                                                                <  1:2977574/150‑1 (MQ=255)
                  aTTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGc                                                                                                                              >  2:4146792/1‑150 (MQ=255)
                   ttCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCg                                                                                                                             >  1:2666921/1‑150 (MQ=255)
                    tCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTAGAGTTACGCCAGGCACCATCAGCGa                                                                                                                            >  2:4268635/1‑150 (MQ=255)
                     cccTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGAt                                                                                                                           <  1:3415031/150‑1 (MQ=255)
                      ccTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATg                                                                                                                          <  2:3350393/150‑1 (MQ=255)
                       cTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGa                                                                                                                         <  2:3186603/150‑1 (MQ=255)
                        tACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGaa                                                                                                                        >  1:4788758/1‑150 (MQ=255)
                        tACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGaa                                                                                                                        >  1:3022071/1‑150 (MQ=255)
                        tACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGaa                                                                                                                        <  1:1387060/150‑1 (MQ=255)
                         aCCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAg                                                                                                                       >  1:3119302/1‑150 (MQ=255)
                         aCCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAg                                                                                                                       <  1:4394618/150‑1 (MQ=255)
                           cATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGACCAAATTGGCGATCCGGCGACCAGCACAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGcc                                                                                                                     >  2:4024550/1‑150 (MQ=255)
                           cATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTCTAGTTACGCCAGGCACCATCAGCGATGAAGcc                                                                                                                     >  2:1461203/1‑150 (MQ=255)
                           cATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGcc                                                                                                                     <  1:5099386/150‑1 (MQ=255)
                               cGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGCTACGCCAGGCACCATCAGCGATGAAGCCCTGt                                                                                                                 <  1:2197424/150‑1 (MQ=255)
                                tgTGGAAAACTATCTCGCCAAACTGGTGAATCATGGAGAGTACGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGACGCCCTGtt                                                                                                                >  2:2286555/2‑150 (MQ=255)
                                ggTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGtt                                                                                                                <  2:5094585/150‑1 (MQ=255)
                                ggTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGtt                                                                                                                <  2:4426763/150‑1 (MQ=255)
                                ggTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGtt                                                                                                                <  1:694438/150‑1 (MQ=255)
                                 gTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTg                                                                                                               <  1:3349697/150‑1 (MQ=255)
                                  tGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGc                                                                                                              <  1:2909582/150‑1 (MQ=255)
                                   ggAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCa                                                                                                             >  2:395984/1‑150 (MQ=255)
                                    gAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAg                                                                                                            >  2:2675732/1‑150 (MQ=255)
                                     aaaaCTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAgg                                                                                                           >  2:1823660/1‑150 (MQ=255)
                                      aaaCTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGa                                                                                                          >  1:444209/1‑150 (MQ=255)
                                        aCTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGc                                                                                                        >  1:5045035/1‑150 (MQ=255)
                                           aTCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTc                                                                                                     <  2:3381615/150‑1 (MQ=255)
                                               cGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGa                                                                                                 >  2:3930668/1‑150 (MQ=255)
                                                 ccAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACa                                                                                               >  2:4535480/1‑150 (MQ=255)
                                                   aaaCTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAAc                                                                                             >  2:208738/1‑150 (MQ=255)
                                                    aaCTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAAcc                                                                                            >  2:676261/1‑150 (MQ=255)
                                                     aCTTGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACct                                                                                           <  2:719873/150‑1 (MQ=255)
                                                     aCTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACct                                                                                           <  2:4243857/150‑1 (MQ=255)
                                                     aCTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACct                                                                                           <  2:865843/150‑1 (MQ=255)
                                                        ggTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACctgct                                                                                        <  2:1180546/150‑1 (MQ=255)
                                                         gTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACctgctg                                                                                       <  1:1121136/150‑1 (MQ=255)
                                                           gAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTggc                                                                                     >  2:3512177/1‑150 (MQ=255)
                                                             aTCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTggcgg                                                                                   >  1:180758/1‑150 (MQ=255)
                                                                 gggAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTAt                                                                               >  2:2408305/1‑150 (MQ=255)
                                                                  ggAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATc                                                                              >  2:1823616/1‑150 (MQ=255)
                                                                  ggAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATc                                                                              <  2:4560962/150‑1 (MQ=255)
                                                                   gagaGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCt                                                                             <  1:4493336/150‑1 (MQ=255)
                                                                    agagTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTg                                                                            <  2:767140/150‑1 (MQ=255)
                                                                    agagTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTg                                                                            <  2:4789519/150‑1 (MQ=255)
                                                                    agagTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTg                                                                            >  2:3608990/1‑150 (MQ=255)
                                                                          cGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGa                                                                      >  1:3673913/1‑150 (MQ=255)
                                                                          cGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGa                                                                      >  1:833216/1‑150 (MQ=255)
                                                                            ttGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACa                                                                    <  2:909842/150‑1 (MQ=255)
                                                                            ttGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACa                                                                    <  1:5701/150‑1 (MQ=255)
                                                                             tGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAg                                                                   >  2:2427195/1‑150 (MQ=255)
                                                                              gCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGc                                                                  <  2:2195824/150‑1 (MQ=255)
                                                                                    tGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGt                                                            >  1:3967990/1‑150 (MQ=255)
                                                                                     gCGAACACATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGtt                                                           <  1:4976877/150‑1 (MQ=255)
                                                                                      cGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGttt                                                          >  2:903056/1‑150 (MQ=255)
                                                                                       gAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTc                                                         >  1:4425153/1‑150 (MQ=255)
                                                                                          cAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGc                                                      <  2:1632864/150‑1 (MQ=255)
                                                                                           aaaTTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCt                                                     <  1:4707852/150‑1 (MQ=255)
                                                                                             aTTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTAc                                                   <  2:1264834/150‑1 (MQ=255)
                                                                                              ttGGCTATCCGGCGACCAGCAAAGGTCAGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTAcg                                                  <  1:3580190/150‑1 (MQ=255)
                                                                                                ggCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTAcgcg                                                <  2:2263876/150‑1 (MQ=255)
                                                                                                   gATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACg                                             >  1:2257857/1‑150 (MQ=255)
                                                                                                        ggCGACCAGCAAAGGTCCGGCTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGa                                        >  1:1678808/1‑150 (MQ=255)
                                                                                                          cGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGata                                      <  2:2005078/150‑1 (MQ=255)
                                                                                                           gACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGatat                                     >  1:3119722/1‑150 (MQ=255)
                                                                                                             ccAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCa                                   <  1:29488/150‑1 (MQ=255)
                                                                                                              cAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTg                                          <  2:5013426/142‑1 (MQ=255)
                                                                                                              cAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAg                                  <  2:3050113/150‑1 (MQ=255)
                                                                                                              cAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAg                                  >  1:4628594/1‑150 (MQ=255)
                                                                                                                gCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGtt                                >  1:2383956/1‑150 (MQ=255)
                                                                                                                   aaGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCg                             <  1:972843/150‑1 (MQ=255)
                                                                                                                      gTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGc                          >  2:4898578/1‑150 (MQ=255)
                                                                                                                       tCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCg                         >  2:2610416/1‑150 (MQ=255)
                                                                                                                          ggTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGttt                      >  2:544866/1‑150 (MQ=255)
                                                                                                                            ttGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTc                    <  1:802538/150‑1 (MQ=255)
                                                                                                                              gAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGc                  <  1:1437690/150‑1 (MQ=255)
                                                                                                                               aGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGcc                 <  2:3113239/150‑1 (MQ=255)
                                                                                                                               aGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGcc                 >  1:2051018/1‑150 (MQ=255)
                                                                                                                                gcgcAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCt                >  2:52066/1‑150 (MQ=255)
                                                                                                                                 cgcAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTg               <  1:1760509/150‑1 (MQ=255)
                                                                                                                                         tgtgCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAAcc       <  1:3747226/150‑1 (MQ=255)
                                                                                                                                          gtgCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCg      <  2:4290909/150‑1 (MQ=255)
                                                                                                                                             cGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCt   <  2:5161585/150‑1 (MQ=255)
                                                                                                                                              gTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTg  >  1:1036003/1‑150 (MQ=255)
                                                                                                                                              gTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTg  <  2:466896/150‑1 (MQ=255)
                                                                                                                                              gTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTg  >  1:4497027/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CCGATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATCGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTG  >  CP009273/2850644‑2850935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAGCCGATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATCGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACC  >  CP009273/2850641‑2850938
                                                                                                                                                     |                                                                                                                                                    
GAGCCGATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATA                                                                                                                                                      <  SRR19973062.2591313/150‑1 (MQ=60)
 AGCCGATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAG                                                                                                                                                     >  SRR19973062.905233/1‑150 (MQ=60)
 AGCCGATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAG                                                                                                                                                     >  SRR19973062.3297966/1‑150 (MQ=60)
 AGCCGATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAAATATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGAGAACACATTGGCGATCCAGCGACCAGCAAAGGTCCGGTGGAGCGCAAAGTTGTGAGTATAG                                                                                                                                                     >  SRR19973062.4280656/1‑150 (MQ=60)
   CCGATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTT                                                                                                                                                   <  SRR19973062.201565/150‑1 (MQ=60)
     GATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTTTAGTTAC                                                                                                                                                 <  SRR19973062.5244610/150‑1 (MQ=60)
     GATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTAC                                                                                                                                                 >  SRR19973062.1292239/1‑150 (MQ=60)
     GATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTAC                                                                                                                                                 <  SRR19973062.2216317/150‑1 (MQ=60)
      ATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACG                                                                                                                                                >  SRR19973062.2299375/1‑150 (MQ=60)
       TCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGC                                                                                                                                               >  SRR19973062.5188854/1‑150 (MQ=60)
        CCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCC                                                                                                                                              <  SRR19973062.2469413/150‑1 (MQ=60)
                 GGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCAT                                                                                                                                     >  SRR19973062.2912003/1‑150 (MQ=60)
                 GGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCAT                                                                                                                                     >  SRR19973062.4233910/1‑150 (MQ=60)
                   GGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCA                                                                                                                                   <  SRR19973062.2992853/150‑1 (MQ=60)
                   GGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCA                                                                                                                                   <  SRR19973062.416441/150‑1 (MQ=60)
                   GGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCA                                                                                                                                   <  SRR19973062.2993189/150‑1 (MQ=60)
                    GATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAG                                                                                                                                  <  SRR19973062.4225619/150‑1 (MQ=60)
                     ATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGC                                                                                                                                 >  SRR19973062.1805076/1‑150 (MQ=60)
                     ATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGC                                                                                                                                 <  SRR19973062.214013/150‑1 (MQ=60)
                      TTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCG                                                                                                                                >  SRR19973062.2680912/1‑150 (MQ=60)
                       TCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGA                                                                                                                               >  SRR19973062.600672/1‑150 (MQ=60)
                        CCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGTT                                                                                                                              <  SRR19973062.84076/150‑1 (MQ=60)
                        CCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGAT                                                                                                                              <  SRR19973062.3432512/150‑1 (MQ=60)
                        CCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGAT                                                                                                                              >  SRR19973062.3035197/1‑150 (MQ=60)
                        CCCTACCATGCGGTGGAAAACTATCACGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGAAAAGTTGTGCGTATAGTTACGCCAGTCACCATCAGCGAT                                                                                                                              >  SRR19973062.3035174/1‑150 (MQ=60)
                         CCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATG                                                                                                                             <  SRR19973062.1276930/150‑1 (MQ=60)
                          CTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGA                                                                                                                            >  SRR19973062.4313080/1‑150 (MQ=60)
                          CTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGA                                                                                                                            >  SRR19973062.4312838/1‑150 (MQ=60)
                           TACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAA                                                                                                                           >  SRR19973062.3037844/1‑150 (MQ=60)
                           TACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAA                                                                                                                           <  SRR19973062.1393590/150‑1 (MQ=60)
                           TACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAA                                                                                                                           <  SRR19973062.1433430/150‑1 (MQ=60)
                           TACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAA                                                                                                                           >  SRR19973062.4813617/1‑150 (MQ=60)
                            ACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAG                                                                                                                          >  SRR19973062.3135356/1‑150 (MQ=60)
                            ACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAG                                                                                                                          <  SRR19973062.4417122/150‑1 (MQ=60)
                            ACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAG                                                                                                                          >  SRR19973062.1223105/1‑150 (MQ=60)
                             CCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGC                                                                                                                         >  SRR19973062.2647685/1‑150 (MQ=60)
                              CATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCC                                                                                                                        <  SRR19973062.5126383/150‑1 (MQ=60)
                              CATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCC                                                                                                                        >  SRR19973062.2430475/1‑150 (MQ=60)
                                TGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCT                                                                                                                      >  SRR19973062.3871344/1‑150 (MQ=60)
                                  CGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGCTACGCCAGGCACCATCAGCGATGAAGCCCTGT                                                                                                                    <  SRR19973062.2208447/150‑1 (MQ=60)
                                   GGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTT                                                                                                                   <  SRR19973062.697644/150‑1 (MQ=60)
                                   GGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTT                                                                                                                   >  SRR19973062.2466994/1‑150 (MQ=60)
                                    GTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTG                                                                                                                  <  SRR19973062.3366957/150‑1 (MQ=60)
                                     TGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGC                                                                                                                 <  SRR19973062.2924926/150‑1 (MQ=60)
                                     TGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGC                                                                                                                 <  SRR19973062.2924919/150‑1 (MQ=60)
                                     TGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGC                                                                                                                 >  SRR19973062.4475544/1‑150 (MQ=60)
                                       GAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAG                                                                                                               >  SRR19973062.1699973/1‑150 (MQ=60)
                                       GAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAG                                                                                                               >  SRR19973062.3420304/1‑150 (MQ=60)
                                       GAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAG                                                                                                               >  SRR19973062.3139501/1‑150 (MQ=60)
                                        AAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGG                                                                                                              >  SRR19973062.4984355/1‑150 (MQ=60)
                                         AAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGA                                                                                                             >  SRR19973062.446104/1‑150 (MQ=60)
                                         AAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGA                                                                                                             >  SRR19973062.445967/1‑150 (MQ=60)
                                           ACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGC                                                                                                           >  SRR19973062.5071783/1‑150 (MQ=60)
                                            CTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCG                                                                                                          >  SRR19973062.4913498/1‑150 (MQ=60)
                                                 TCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGG                                                                                                     <  SRR19973062.1377148/150‑1 (MQ=60)
                                                 TCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGG                                                                                                     >  SRR19973062.1887449/1‑150 (MQ=60)
                                                       AACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACC                                                                                               <  SRR19973062.2934777/150‑1 (MQ=60)
                                                       AACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACC                                                                                               <  SRR19973062.4110349/150‑1 (MQ=60)
                                                        ACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCT                                                                                              <  SRR19973062.607641/150‑1 (MQ=60)
                                                        ACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCT                                                                                              <  SRR19973062.3554751/150‑1 (MQ=60)
                                                         CTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTG                                                                                             <  SRR19973062.4473622/150‑1 (MQ=60)
                                                           GGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCT                                                                                           <  SRR19973062.3631701/150‑1 (MQ=60)
                                                            GTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTG                                                                                          <  SRR19973062.1126392/150‑1 (MQ=60)
                                                            GTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTG                                                                                          <  SRR19973062.838692/150‑1 (MQ=60)
                                                               AATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCG                                                                                       >  SRR19973062.4626135/1‑150 (MQ=60)
                                                                ATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGG                                                                                      >  SRR19973062.181746/1‑150 (MQ=60)
                                                                      GAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCT                                                                                <  SRR19973062.4516634/150‑1 (MQ=60)
                                                                      GAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCT                                                                                <  SRR19973062.4516528/150‑1 (MQ=60)
                                                                          GTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCA                                                                            >  SRR19973062.4771804/1‑150 (MQ=60)
                                                                            CCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGG                                                                          >  SRR19973062.468975/1‑150 (MQ=60)
                                                                             CGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGA                                                                         >  SRR19973062.3692750/1‑150 (MQ=60)
                                                                             CGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGA                                                                         >  SRR19973062.3107250/1‑150 (MQ=60)
                                                                             CGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGA                                                                         >  SRR19973062.836863/1‑150 (MQ=60)
                                                                               TTGCCATCTGCGAACATTTTGGCGATCCGGCGACCAGCTTTGGTCCGGTTGAGCGCTTAGTTGTGCGTTTAGTTTCGCCTGGCACCATCAGCTATGAAGCCCTGTTGCTGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACA                                                                       <  SRR19973062.3716952/150‑1 (MQ=60)
                                                                               TTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACA                                                                       <  SRR19973062.5742/150‑1 (MQ=60)
                                                                               TTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACA                                                                       <  SRR19973062.5213988/150‑1 (MQ=60)
                                                                                  CCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCA                                                                    <  SRR19973062.501908/150‑1 (MQ=60)
                                                                                    ATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAA                                                                  >  SRR19973062.2941961/1‑150 (MQ=60)
                                                                                      CTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGG                                                                >  SRR19973062.4182492/1‑150 (MQ=60)
                                                                                       TGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGT                                                               <  SRR19973062.452421/150‑1 (MQ=60)
                                                                                       TGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGT                                                               >  SRR19973062.3988097/1‑150 (MQ=60)
                                                                                        GCGAACACATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTT                                                              <  SRR19973062.5003125/150‑1 (MQ=60)
                                                                                          GAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCACAGGTTTC                                                            >  SRR19973062.4447760/1‑150 (MQ=60)
                                                                                          GAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTC                                                            >  SRR19973062.4447755/1‑150 (MQ=60)
                                                                                           AACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCG                                                           <  SRR19973062.3289288/150‑1 (MQ=60)
                                                                                            ACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGG                                                          <  SRR19973062.3612516/150‑1 (MQ=60)
                                                                                              AAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCT                                                        <  SRR19973062.1694893/150‑1 (MQ=60)
                                                                                              AAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCT                                                        <  SRR19973062.4732431/150‑1 (MQ=60)
                                                                                              AAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCT                                                        <  SRR19973062.1482679/150‑1 (MQ=60)
                                                                                                 TTGGCTATCCGGCGACCAGCAAAGGTCAGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACG                                                     <  SRR19973062.3598283/150‑1 (MQ=60)
                                                                                                     CGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGAC                                                 >  SRR19973062.306042/1‑150 (MQ=60)
                                                                                                      GATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACG                                                >  SRR19973062.2269197/1‑150 (MQ=60)
                                                                                                        TCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCT                                              >  SRR19973062.2082893/1‑150 (MQ=60)
                                                                                                           GGCGACCAGCAAAGGTCCGGTTGAGCGCAACGTTGTGCGTATAGTTACGCCAGGTACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGA                                           <  SRR19973062.5280940/150‑1 (MQ=60)
                                                                                                           GGCGACCAGCAAAGGTCCGGCTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGA                                           >  SRR19973062.1687056/1‑150 (MQ=60)
                                                                                                              GACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATAT                                        >  SRR19973062.3135777/1‑150 (MQ=60)
                                                                                                              GACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATAT                                        <  SRR19973062.2275526/150‑1 (MQ=60)
                                                                                                                CCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCA                                      <  SRR19973062.29711/150‑1 (MQ=60)
                                                                                                                 CAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGagatcgga                                     >  SRR19973062.5039962/1‑142 (MQ=60)
                                                                                                                 CAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAG                                     >  SRR19973062.4652817/1‑150 (MQ=60)
                                                                                                                  AGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGT                                    >  SRR19973062.372816/1‑150 (MQ=60)
                                                                                                                   GCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTT                                   >  SRR19973062.2396733/1‑150 (MQ=60)
                                                                                                                    CAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCGGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTC                                  >  SRR19973062.757179/1‑150 (MQ=60)
                                                                                                                     AAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCC                                 >  SRR19973062.4445796/1‑150 (MQ=60)
                                                                                                                     AAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCC                                 >  SRR19973062.5085259/1‑150 (MQ=60)
                                                                                                                      AAGGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCG                                <  SRR19973062.977333/150‑1 (MQ=60)
                                                                                                                        GGTCCGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGG                              <  SRR19973062.78280/150‑1 (MQ=60)
                                                                                                                            CGGTTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTT                          >  SRR19973062.1049649/1‑150 (MQ=60)
                                                                                                                              GTTGAGCGCAAAGTTGTGCGTATAGTTCCGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTT                        <  SRR19973062.1334027/150‑1 (MQ=60)
                                                                                                                               TTGAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTC                       <  SRR19973062.806095/150‑1 (MQ=60)
                                                                                                                                 GAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGC                     <  SRR19973062.1444668/150‑1 (MQ=60)
                                                                                                                                 GAGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGC                     <  SRR19973062.3135612/150‑1 (MQ=60)
                                                                                                                                  AGCGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCC                    >  SRR19973062.2061523/1‑150 (MQ=60)
                                                                                                                                    CGCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTG                  <  SRR19973062.1769049/150‑1 (MQ=60)
                                                                                                                                     GCAAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGA                 >  SRR19973062.155844/1‑150 (MQ=60)
                                                                                                                                       AAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGC               >  SRR19973062.5157771/1‑150 (MQ=60)
                                                                                                                                       AAAGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGC               >  SRR19973062.5157750/1‑150 (MQ=60)
                                                                                                                                         AGTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGA             <  SRR19973062.384946/150‑1 (MQ=60)
                                                                                                                                          GTTGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAA            >  SRR19973062.5214407/1‑150 (MQ=60)
                                                                                                                                            TGTGCGTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACC          <  SRR19973062.3766449/150‑1 (MQ=60)
                                                                                                                                                 GTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTG     >  SRR19973062.3405081/1‑150 (MQ=60)
                                                                                                                                                 GTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTG     >  SRR19973062.822144/1‑150 (MQ=60)
                                                                                                                                                 GTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTG     >  SRR19973062.4520264/1‑150 (MQ=60)
                                                                                                                                                 GTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTG     >  SRR19973062.4682273/1‑150 (MQ=60)
                                                                                                                                                 GTATAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTG     >  SRR19973062.1040853/1‑150 (MQ=60)
                                                                                                                                                    TAGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACC  <  SRR19973062.421112/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
GAGCCGATCCCGATGGCGGGGATTCCCTACCATGCGGTGGAAAACTATCTCGCCAAACTGGTGAATCAGGGAGAGTCCGTTGCCATCTGCGAACAAATTGGCGATCCGGCGACCAGCAAAGGTCCGGTTGAGCGCAAAGTTGTGCGTATCGTTACGCCAGGCACCATCAGCGATGAAGCCCTGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACC  >  CP009273/2850641‑2850938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑