Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,440,151 G→T 100% I31I (ATC→ATA)  rpsN ← 30S ribosomal subunit protein S14

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,440,1510GT99.1% 740.9 / ‑2.1 228I31I (ATC→ATA) rpsN30S ribosomal subunit protein S14
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (94/132);  total (96/132)
Fisher's exact test for biased strand distribution p-value = 1.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 242 total aligned reads displayed.

CAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAG  >  CP009273/3440007‑3440295
                                                                                                                                                |                                                                                                                                                
cAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAtatg                                                                                                                                               >  2:2915374/1‑148 (MQ=255)
 aaCCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAtatga                                                                                                                                              >  2:1760352/1‑148 (MQ=255)
 aaCCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAtatga                                                                                                                                              >  2:329587/1‑148 (MQ=255)
  aCCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAtatgat                                                                                                                                             <  1:1158081/148‑1 (MQ=255)
  aCCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAtatgat                                                                                                                                             >  2:2430728/1‑148 (MQ=255)
   cccGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATc                                                                                                                                            >  1:317872/1‑148 (MQ=255)
    ccGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCg                                                                                                                                           <  1:635698/148‑1 (MQ=255)
        acgtCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCttt                                                                                                                                       <  2:1932828/145‑1 (MQ=255)
        aCTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCttt                                                                                                                                       <  2:2808244/148‑1 (MQ=255)
        aCTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCttt                                                                                                                                       <  2:3263046/148‑1 (MQ=255)
                aGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTttcagttcag                                                                                                                               <  2:156400/148‑1 (MQ=255)
                   aaaCCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAgcgc                                                                                                                            >  2:899063/1‑148 (MQ=255)
                   aaaCCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAgcgc                                                                                                                            >  1:1719610/1‑148 (MQ=255)
                     aCCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGt                                                                                                                          <  1:517832/148‑1 (MQ=255)
                      ccATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGtt                                                                                                                         >  1:2290565/1‑148 (MQ=255)
                      ccATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGtt                                                                                                                         >  1:3155319/1‑148 (MQ=255)
                          gCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTcgc                                                                                                                     >  1:1578112/1‑148 (MQ=255)
                          gCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTcgc                                                                                                                     >  1:2259365/1‑148 (MQ=255)
                           cGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTcgcg                                                                                                                    <  1:2455458/148‑1 (MQ=255)
                             gacgacCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGaa                                                                                                                  >  1:2574484/1‑148 (MQ=255)
                              acgacCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAg                                                                                                                 <  2:504530/148‑1 (MQ=255)
                              acgacCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAg                                                                                                                 >  2:70401/1‑148 (MQ=255)
                              acgacCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAg                                                                                                                 >  1:1366041/1‑148 (MQ=255)
                               cgacCTGATGGACGGCAGCGGGTACGCTGACGAGACGGGCTGGAATCACGCGCCAGAGTCTGCAGCTTGAGAACCGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGt                                                                                                                <  1:1839416/148‑1 (MQ=255)
                                gacCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTa                                                                                                               <  2:3033081/148‑1 (MQ=255)
                                gacCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTACAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTa                                                                                                               <  1:1294796/148‑1 (MQ=255)
                                   cTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTAttt                                                                                                            <  1:3337696/148‑1 (MQ=255)
                                    tGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTa                                                                                                           >  1:587583/1‑148 (MQ=255)
                                    tGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTa                                                                                                           <  2:2811368/148‑1 (MQ=255)
                                        tGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAg                                                                                                       <  1:3009859/148‑1 (MQ=255)
                                         gACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGc                                                                                                      <  1:2773601/148‑1 (MQ=255)
                                          aCGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCt                                                                                                     >  1:3462091/1‑148 (MQ=255)
                                          aCGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGGTTCGCGAAGTATTTATCAGCt                                                                                                     >  2:1645138/1‑148 (MQ=255)
                                            ggCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTaa                                                                                                   <  1:673804/148‑1 (MQ=255)
                                            ggCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTaa                                                                                                   <  1:894970/148‑1 (MQ=255)
                                            ggCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTaa                                                                                                   <  1:2688466/148‑1 (MQ=255)
                                             gCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACAGCAGATATGATCGCTATCAGTTCAGCGCGTGTCGCGAAGTATTTATCAGCTaaa                                                                                                  >  1:1020213/1‑148 (MQ=255)
                                              cAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAg                                                                                                 >  2:580743/1‑148 (MQ=255)
                                              cAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAg                                                                                                 <  2:1833113/148‑1 (MQ=255)
                                              cAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAg                                                                                                 >  1:2819690/1‑148 (MQ=255)
                                               aGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGc                                                                                                <  1:2835247/148‑1 (MQ=255)
                                                 cGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTa                                                                                              <  2:3200204/148‑1 (MQ=255)
                                                     tACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTAcgcg                                                                                          >  2:1541925/1‑148 (MQ=255)
                                                     tACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTAcgcg                                                                                          <  1:639003/148‑1 (MQ=255)
                                                      aCGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGt                                                                                         <  2:1585055/148‑1 (MQ=255)
                                                      aCGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGt                                                                                         <  2:1584946/148‑1 (MQ=255)
                                                          tGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTa                                                                                     <  2:2140325/148‑1 (MQ=255)
                                                             cGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACtt                                                                                  >  2:2956572/1‑148 (MQ=255)
                                                               agaCGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTcg                                                                                >  2:1078315/1‑148 (MQ=255)
                                                                 aCGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTcgcg                                                                              <  2:955699/148‑1 (MQ=255)
                                                                       tGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCttt                                                                        <  1:1952680/148‑1 (MQ=255)
                                                                        ggAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTc                                                                       >  2:2238115/1‑148 (MQ=255)
                                                                        ggAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTc                                                                       <  2:2836899/148‑1 (MQ=255)
                                                                        ggAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTc                                                                       <  2:1525695/148‑1 (MQ=255)
                                                                         gAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCa                                                                      <  1:1332645/148‑1 (MQ=255)
                                                                         gAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCa                                                                      <  1:1089229/148‑1 (MQ=255)
                                                                          aaTCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCat                                                                     >  2:2049040/1‑148 (MQ=255)
                                                                             cACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCattga                                                                  <  2:1221177/148‑1 (MQ=255)
                                                                              aCGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCattgat                                                                 <  2:288493/148‑1 (MQ=255)
                                                                                 cgGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGc                                                              >  1:1436704/1‑148 (MQ=255)
                                                                                  ggCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCt                                                             <  1:517450/148‑1 (MQ=255)
                                                                                   gCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCtt                                                            <  1:178568/148‑1 (MQ=255)
                                                                                       agTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGcc                                                        >  2:2180662/1‑148 (MQ=255)
                                                                                        gTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCa                                                       <  1:329587/148‑1 (MQ=255)
                                                                                         tCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCAt                                                      >  1:3225345/1‑148 (MQ=255)
                                                                                         tCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCAt                                                      <  2:408147/148‑1 (MQ=255)
                                                                                         tCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCAt                                                      >  2:3396299/1‑148 (MQ=255)
                                                                                         tCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCAt                                                      >  1:923008/1‑148 (MQ=255)
                                                                                            gCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTa                                                   >  2:45394/1‑148 (MQ=255)
                                                                                            gCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTa                                                   <  1:1747809/148‑1 (MQ=255)
                                                                                             cAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAg                                                  <  1:35215/148‑1 (MQ=255)
                                                                                             cAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAg                                                  >  1:124326/1‑148 (MQ=255)
                                                                                               gCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTa                                                >  2:2120550/1‑148 (MQ=255)
                                                                                                  tGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAAcc                                             <  1:57648/148‑1 (MQ=255)
                                                                                                   gagaACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAAccc                                            <  2:1724667/148‑1 (MQ=255)
                                                                                                    agaACCGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCt                                           >  2:226367/1‑148 (MQ=255)
                                                                                                     gaACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTa                                          <  2:2469707/148‑1 (MQ=255)
                                                                                                       aCAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTAcc                                        <  2:1817727/148‑1 (MQ=255)
                                                                                                          gCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTa                                     <  1:3293212/148‑1 (MQ=255)
                                                                                                          gCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTa                                     <  1:1552896/148‑1 (MQ=255)
                                                                                                          gCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTa                                     <  2:3147274/148‑1 (MQ=255)
                                                                                                                cAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCg                               <  2:112355/148‑1 (MQ=255)
                                                                                                                  aCGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGa                             >  1:1344851/1‑148 (MQ=255)
                                                                                                                   cGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGaa                            >  1:1050909/1‑148 (MQ=255)
                                                                                                                     aTCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACg                          <  2:2963834/148‑1 (MQ=255)
                                                                                                                      tCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACgg                         >  2:264735/1‑148 (MQ=255)
                                                                                                                        ttCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGa                       <  1:2753898/148‑1 (MQ=255)
                                                                                                                            tcgGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTc                   >  2:2910267/1‑148 (MQ=255)
                                                                                                                                aaGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAg               >  1:3213343/1‑148 (MQ=255)
                                                                                                                                  gCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTTATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGc             >  2:488995/1‑148 (MQ=255)
                                                                                                                                  gCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGc             <  1:2544778/148‑1 (MQ=255)
                                                                                                                                     ttCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGcagc          >  1:237679/1‑148 (MQ=255)
                                                                                                                                     ttCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGcagc          >  2:2599828/1‑148 (MQ=255)
                                                                                                                                      tCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGcagcc         >  1:2121969/1‑148 (MQ=255)
                                                                                                                                      tCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGcagcc         >  2:2126445/1‑148 (MQ=255)
                                                                                                                                        acaTCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGcagccag       <  2:1342687/148‑1 (MQ=255)
                                                                                                                                        acaTCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGcagccag       >  2:1440091/1‑148 (MQ=255)
                                                                                                                                         caTCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGcagccagc      >  2:3388394/1‑148 (MQ=255)
                                                                                                                                             agatATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCagag  <  2:367370/148‑1 (MQ=255)
                                                                                                                                             agatATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCagag  <  2:1366041/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAG  >  CP009273/3440007‑3440295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGC  >  CP009273/3440003‑3440298
                                                                                                                                                    |                                                                                                                                                   
GGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATA                                                                                                                                                    >  SRR19973065.226829/1‑150 (MQ=60)
    CAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGAGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGAT                                                                                                                                                <  SRR19973065.282688/150‑1 (MQ=60)
     AACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATC                                                                                                                                               >  SRR19973065.320056/1‑150 (MQ=60)
     AACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATC                                                                                                                                               >  SRR19973065.320040/1‑150 (MQ=60)
      ACCCTAACTTCCGCAGGAAACCATGCGGTCGAGATGTTTTTCGGCATCGGTTACGCTGACGAGACGGGATGGAATCACGCGGCATAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCG                                                                                                                                              <  SRR19973065.1831831/150‑1 (MQ=60)
      ACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCG                                                                                                                                              <  SRR19973065.1664322/150‑1 (MQ=60)
      ACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCG                                                                                                                                              <  SRR19973065.1165508/150‑1 (MQ=60)
        CCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCT                                                                                                                                            <  SRR19973065.639850/150‑1 (MQ=60)
               TCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTT                                                                                                                                     <  SRR19973065.1723285/150‑1 (MQ=60)
                   CAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGC                                                                                                                                 >  SRR19973065.884421/1‑150 (MQ=60)
                     GGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGC                                                                                                                               >  SRR19973065.1731035/1‑150 (MQ=60)
                        AACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTT                                                                                                                            >  SRR19973065.2304892/1‑150 (MQ=60)
                        AACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTT                                                                                                                            >  SRR19973065.2304998/1‑150 (MQ=60)
                        AACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTT                                                                                                                            >  SRR19973065.3174457/1‑150 (MQ=60)
                        AACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTT                                                                                                                            >  SRR19973065.3173906/1‑150 (MQ=60)
                         ACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTT                                                                                                                           <  SRR19973065.521254/150‑1 (MQ=60)
                         ACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTT                                                                                                                           <  SRR19973065.521120/150‑1 (MQ=60)
                            ATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGC                                                                                                                        >  SRR19973065.2273653/1‑150 (MQ=60)
                            ATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGC                                                                                                                        >  SRR19973065.1588632/1‑150 (MQ=60)
                               CGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAA                                                                                                                     >  SRR19973065.2590546/1‑150 (MQ=60)
                               CGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAA                                                                                                                     <  SRR19973065.2470934/150‑1 (MQ=60)
                               CGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGCCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAA                                                                                                                     <  SRR19973065.364196/150‑1 (MQ=60)
                                GGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAG                                                                                                                    >  SRR19973065.1375072/1‑150 (MQ=60)
                                GGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAG                                                                                                                    <  SRR19973065.2446065/150‑1 (MQ=60)
                                   CGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTAT                                                                                                                 <  SRR19973065.798175/150‑1 (MQ=60)
                                   CGACCTGATGGACGGCAGCGGGTACGCTGACGAGACGGGCTGGAATCACGCGCCAGAGTCTGCAGCTTGAGAACCGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTAT                                                                                                                 <  SRR19973065.1851546/150‑1 (MQ=60)
                                    GACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATT                                                                                                                >  SRR19973065.1497351/1‑150 (MQ=60)
                                    GACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTACAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATT                                                                                                                <  SRR19973065.1303325/150‑1 (MQ=60)
                                    GACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTACAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATT                                                                                                                <  SRR19973065.1303326/150‑1 (MQ=60)
                                      CCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTA                                                                                                              <  SRR19973065.348764/150‑1 (MQ=60)
                                      CCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTA                                                                                                              >  SRR19973065.2268458/1‑150 (MQ=60)
                                      CCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTA                                                                                                              >  SRR19973065.591235/1‑150 (MQ=60)
                                      CCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTA                                                                                                              >  SRR19973065.2850142/1‑150 (MQ=60)
                                      CCTGATTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTA                                                                                                              <  SRR19973065.2129593/150‑1 (MQ=60)
                                       CTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTAT                                                                                                             <  SRR19973065.3358077/150‑1 (MQ=60)
                                        TGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATC                                                                                                            >  SRR19973065.1330104/1‑150 (MQ=60)
                                         GTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCTGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCA                                                                                                           >  SRR19973065.2133799/1‑150 (MQ=60)
                                          TTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAG                                                                                                          <  SRR19973065.1047924/150‑1 (MQ=60)
                                            TGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCT                                                                                                        >  SRR19973065.3483179/1‑150 (MQ=60)
                                            TGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCT                                                                                                        <  SRR19973065.3028292/150‑1 (MQ=60)
                                            TGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCT                                                                                                        >  SRR19973065.1736109/1‑150 (MQ=60)
                                             GACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTA                                                                                                       <  SRR19973065.2790825/150‑1 (MQ=60)
                                             GACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTA                                                                                                       <  SRR19973065.2790805/150‑1 (MQ=60)
                                               CGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACAGCAGATATGATCGCTATCAGTTCAGCGCGTGTCGCGAAGTATTTATCAGCTAAA                                                                                                     >  SRR19973065.1026976/1‑150 (MQ=60)
                                                GGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAG                                                                                                    <  SRR19973065.2705155/150‑1 (MQ=60)
                                                GGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAG                                                                                                    <  SRR19973065.678266/150‑1 (MQ=60)
                                                GGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAG                                                                                                    <  SRR19973065.678293/150‑1 (MQ=60)
                                                GGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAG                                                                                                    >  SRR19973065.2837109/1‑150 (MQ=60)
                                                GGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAG                                                                                                    <  SRR19973065.2705267/150‑1 (MQ=60)
                                                GGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAG                                                                                                    <  SRR19973065.2483543/150‑1 (MQ=60)
                                                GGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAG                                                                                                    <  SRR19973065.900727/150‑1 (MQ=60)
                                                GGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAG                                                                                                    <  SRR19973065.900664/150‑1 (MQ=60)
                                                  CAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCT                                                                                                  <  SRR19973065.1366613/150‑1 (MQ=60)
                                                   AGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTA                                                                                                 <  SRR19973065.2852709/150‑1 (MQ=60)
                                                      GGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGC                                                                                              >  SRR19973065.1351568/1‑150 (MQ=60)
                                                       GTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCG                                                                                             >  SRR19973065.33337/1‑150 (MQ=60)
                                                         TACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTT                                                                                           <  SRR19973065.643186/150‑1 (MQ=60)
                                                         TACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTT                                                                                           <  SRR19973065.642720/150‑1 (MQ=60)
                                                                 CGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCG                                                                                   >  SRR19973065.567100/1‑150 (MQ=60)
                                                                 CGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCG                                                                                   >  SRR19973065.567098/1‑150 (MQ=60)
                                                                     ACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTG                                                                               <  SRR19973065.342424/150‑1 (MQ=60)
                                                                        GGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTT                                                                            <  SRR19973065.3775/150‑1 (MQ=60)
                                                                        GGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTT                                                                            <  SRR19973065.3329676/150‑1 (MQ=60)
                                                                          CTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTC                                                                          >  SRR19973065.1767948/1‑150 (MQ=60)
                                                                           TGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCA                                                                         <  SRR19973065.1965073/150‑1 (MQ=60)
                                                                           TGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCA                                                                         <  SRR19973065.1965440/150‑1 (MQ=60)
                                                                            GGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGGTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCAT                                                                        >  SRR19973065.1439934/1‑150 (MQ=60)
                                                                             GAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATT                                                                       <  SRR19973065.1096406/150‑1 (MQ=60)
                                                                             GAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATT                                                                       <  SRR19973065.2341477/150‑1 (MQ=60)
                                                                             GAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATT                                                                       <  SRR19973065.1341465/150‑1 (MQ=60)
                                                                                   CGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGC                                                                 >  SRR19973065.1446027/1‑150 (MQ=60)
                                                                                   CGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGC                                                                 >  SRR19973065.3199851/1‑150 (MQ=60)
                                                                                      GGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTA                                                              <  SRR19973065.635345/150‑1 (MQ=60)
                                                                                      GGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTA                                                              <  SRR19973065.520869/150‑1 (MQ=60)
                                                                                       GCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAG                                                             <  SRR19973065.179977/150‑1 (MQ=60)
                                                                                        CAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGC                                                            <  SRR19973065.2321600/150‑1 (MQ=60)
                                                                                         AGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCC                                                           >  SRR19973065.113361/1‑150 (MQ=60)
                                                                                           AGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCAT                                                         >  SRR19973065.929057/1‑150 (MQ=60)
                                                                                           AGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCAT                                                         >  SRR19973065.3243647/1‑150 (MQ=60)
                                                                                           AGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCAT                                                         >  SRR19973065.3244711/1‑150 (MQ=60)
                                                                                           AGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCAT                                                         >  SRR19973065.1456787/1‑150 (MQ=60)
                                                                                            GTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATT                                                        <  SRR19973065.331986/150‑1 (MQ=60)
                                                                                            GTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATT                                                        <  SRR19973065.331884/150‑1 (MQ=60)
                                                                                            GTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATT                                                        <  SRR19973065.331888/150‑1 (MQ=60)
                                                                                               TGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAG                                                     >  SRR19973065.125403/1‑150 (MQ=60)
                                                                                               TGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAG                                                     >  SRR19973065.1077056/1‑150 (MQ=60)
                                                                                               TGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAG                                                     >  SRR19973065.124658/1‑150 (MQ=60)
                                                                                                GCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGT                                                    <  SRR19973065.1759326/150‑1 (MQ=60)
                                                                                                 CAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTA                                                   <  SRR19973065.35651/150‑1 (MQ=60)
                                                                                                     TTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCC                                               >  SRR19973065.3189704/1‑150 (MQ=60)
                                                                                                      TGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCT                                              <  SRR19973065.58265/150‑1 (MQ=60)
                                                                                                      TGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCT                                              <  SRR19973065.58264/150‑1 (MQ=60)
                                                                                                      TGAGAACAGCGTTACTACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCT                                              <  SRR19973065.97991/150‑1 (MQ=60)
                                                                                                       GAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTA                                             >  SRR19973065.251084/1‑150 (MQ=60)
                                                                                                       GAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTA                                             >  SRR19973065.2799821/1‑150 (MQ=60)
                                                                                                         GAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACC                                           >  SRR19973065.1138976/1‑150 (MQ=60)
                                                                                                          AACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCT                                          <  SRR19973065.1808732/150‑1 (MQ=60)
                                                                                                              GCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACT                                      <  SRR19973065.3313234/150‑1 (MQ=60)
                                                                                                              GCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACT                                      <  SRR19973065.1563193/150‑1 (MQ=60)
                                                                                                              GCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACT                                      <  SRR19973065.3313266/150‑1 (MQ=60)
                                                                                                                    CAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGA                                >  SRR19973065.1353743/1‑150 (MQ=60)
                                                                                                                     AACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAA                               >  SRR19973065.1057869/1‑150 (MQ=60)
                                                                                                                       CGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACG                             >  SRR19973065.2702661/1‑150 (MQ=60)
                                                                                                                        GATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGACCGG                            >  SRR19973065.597900/1‑150 (MQ=60)
                                                                                                                           CTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAA                         <  SRR19973065.2989042/150‑1 (MQ=60)
                                                                                                                            TTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAG                        <  SRR19973065.2771030/150‑1 (MQ=60)
                                                                                                                                  GGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAG                  <  SRR19973065.1587719/150‑1 (MQ=60)
                                                                                                                                  GGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAG                  >  SRR19973065.3232671/1‑150 (MQ=60)
                                                                                                                                      GCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAG              <  SRR19973065.2560718/150‑1 (MQ=60)
                                                                                                                                       CGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGC             >  SRR19973065.2914593/1‑150 (MQ=60)
                                                                                                                                       CGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGC             >  SRR19973065.239386/1‑150 (MQ=60)
                                                                                                                                       CGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGC             >  SRR19973065.1260484/1‑150 (MQ=60)
                                                                                                                                        GTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCC            >  SRR19973065.2135631/1‑150 (MQ=60)
                                                                                                                                         TTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCA           <  SRR19973065.2609211/150‑1 (MQ=60)
                                                                                                                                          TCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAG          >  SRR19973065.1373488/1‑150 (MQ=60)
                                                                                                                                               TCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAG     <  SRR19973065.2573345/150‑1 (MQ=60)
                                                                                                                                                  GATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGC  >  SRR19973065.944044/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
GGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGC  >  CP009273/3440003‑3440298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑