Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,440,151 G→T 89.5% I31I (ATC→ATA)  rpsN ← 30S ribosomal subunit protein S14

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,440,1510GT89.5% 648.6 / 64.8 229I31I (ATC→ATA) rpsN30S ribosomal subunit protein S14
Reads supporting (aligned to +/- strand):  ref base G (14/10);  new base T (101/104);  total (115/114)
Fisher's exact test for biased strand distribution p-value = 5.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01

Only 100 of 239 total aligned reads displayed.

GCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCG  >  CP009273/3440004‑3440299
                                                                                                                                                   |                                                                                                                                                    
gCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGatat                                                                                                                                                    <  2:789447/150‑1 (MQ=255)
  tCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAtatga                                                                                                                                                  <  2:1434863/150‑1 (MQ=255)
   cAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAtatgat                                                                                                                                                 >  2:1091366/1‑150 (MQ=255)
   cAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAtatgat                                                                                                                                                 >  2:432586/1‑150 (MQ=255)
    aaCCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATc                                                                                                                                                <  2:116125/150‑1 (MQ=255)
     aCCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATCTGATCg                                                                                                                                               >  1:4561354/1‑150 (MQ=255)
       ccGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCt                                                                                                                                             <  1:411302/150‑1 (MQ=255)
         gAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCCGATATGATCGCttt                                                                                                                                           >  1:624751/1‑150 (MQ=255)
               ccGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTttcagttc                                                                                                                                     <  1:1637692/150‑1 (MQ=255)
                cGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTttcagttca                                                                                                                                    <  2:2380657/150‑1 (MQ=255)
                       aaCCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGtt                                                                                                                             <  2:1605445/150‑1 (MQ=255)
                        aCCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGttt                                                                                                                            <  1:3963062/150‑1 (MQ=255)
                           aTGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTcgc                                                                                                                         <  2:2014165/150‑1 (MQ=255)
                             gCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGa                                                                                                                       >  2:1632440/1‑150 (MQ=255)
                             gCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGa                                                                                                                       <  2:2764795/150‑1 (MQ=255)
                                 acgacCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTa                                                                                                                   <  1:1315796/150‑1 (MQ=255)
                                 acgacCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTa                                                                                                                   >  1:2888226/1‑150 (MQ=255)
                                  cgacCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGACGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTAt                                                                                                                  >  2:433265/1‑150 (MQ=255)
                                    acCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTAttt                                                                                                                <  2:53109/150‑1 (MQ=255)
                                    acCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTAttt                                                                                                                <  2:4218638/150‑1 (MQ=255)
                                    acCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTAttt                                                                                                                >  2:2264041/1‑150 (MQ=255)
                                    acCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTAttt                                                                                                                <  2:2690715/150‑1 (MQ=255)
                                       tGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATc                                                                                                             <  2:689604/150‑1 (MQ=255)
                                            gACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTa                                                                                                        >  2:4097109/1‑150 (MQ=255)
                                            gACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTa                                                                                                        >  1:3127165/1‑150 (MQ=255)
                                            gACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTa                                                                                                        <  2:3216724/150‑1 (MQ=255)
                                               ggCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAg                                                                                                     <  2:4815153/150‑1 (MQ=255)
                                               ggCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAg                                                                                                     <  2:3143784/150‑1 (MQ=255)
                                                gCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGc                                                                                                    <  1:3905160/150‑1 (MQ=255)
                                                  aGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTa                                                                                                  <  2:3455353/150‑1 (MQ=255)
                                                     ggTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTAcgc                                                                                               >  2:3226677/1‑150 (MQ=255)
                                                        tACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGtt                                                                                            >  1:1688939/1‑150 (MQ=255)
                                                        tACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGtt                                                                                            >  1:324428/1‑150 (MQ=255)
                                                         aCGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGttt                                                                                           <  2:696049/150‑1 (MQ=255)
                                                         aCGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGttt                                                                                           >  2:2973555/1‑150 (MQ=255)
                                                         aCGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGttt                                                                                           >  2:2859253/1‑150 (MQ=255)
                                                         aCGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGttt                                                                                           >  2:2859312/1‑150 (MQ=255)
                                                         aCGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGttt                                                                                           >  1:587414/1‑150 (MQ=255)
                                                             tGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACt                                                                                       <  2:3697954/150‑1 (MQ=255)
                                                                cGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTcg                                                                                    >  2:2578941/1‑150 (MQ=255)
                                                                 gagaCGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGATTTACTTcgc                                                                                   >  1:2636412/1‑150 (MQ=255)
                                                                   gaCGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGt                                                                                 <  1:2859312/150‑1 (MQ=255)
                                                                   gaCGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGt                                                                                 >  1:5039623/1‑150 (MQ=255)
                                                                   gaCGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGt                                                                                 <  1:2859253/150‑1 (MQ=255)
                                                                   gaCGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGt                                                                                 <  1:2539918/150‑1 (MQ=255)
                                                                      gggCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCt                                                                              <  2:4904503/150‑1 (MQ=255)
                                                                       ggCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCtt                                                                             >  1:363276/1‑150 (MQ=255)
                                                                         cTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTc                                                                           <  2:1472734/150‑1 (MQ=255)
                                                                           ggAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCat                                                                         <  2:5068193/150‑1 (MQ=255)
                                                                           ggAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCat                                                                         <  2:1364381/150‑1 (MQ=255)
                                                                            gAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCatt                                                                        >  1:3488373/1‑150 (MQ=255)
                                                                              aTCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCattga                                                                      >  2:1581786/1‑150 (MQ=255)
                                                                                 aCGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCattgattg                                                                   <  1:4339769/150‑1 (MQ=255)
                                                                                      gCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAg                                                              <  1:8706/150‑1 (MQ=255)
                                                                                       cAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGc                                                             <  1:4506447/150‑1 (MQ=255)
                                                                                       cAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGc                                                             <  1:1257911/150‑1 (MQ=255)
                                                                                         gagTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCa                                                           >  1:3772668/1‑150 (MQ=255)
                                                                                          agTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCAt                                                          >  2:370908/1‑150 (MQ=255)
                                                                                           gTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCAtt                                                         <  2:1227288/150‑1 (MQ=255)
                                                                                              tGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAg                                                      >  2:3856633/1‑150 (MQ=255)
                                                                                              tGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAg                                                      >  1:4017322/1‑150 (MQ=255)
                                                                                               gCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGt                                                     >  1:1293044/1‑150 (MQ=255)
                                                                                               gCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGt                                                     >  1:483994/1‑150 (MQ=255)
                                                                                                 aGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTaa                                                   >  2:1888169/1‑150 (MQ=255)
                                                                                                 aGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTaa                                                   >  2:1880535/1‑150 (MQ=255)
                                                                                                  gCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAAc                                                  >  2:1777684/1‑150 (MQ=255)
                                                                                                    ttGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAAccc                                                <  1:3465305/150‑1 (MQ=255)
                                                                                                       agaACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGATTAGCCATTTAGTAACCCTAc                                             >  2:4560677/1‑150 (MQ=255)
                                                                                                          aCAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCtt                                          <  1:104745/150‑1 (MQ=255)
                                                                                                           cAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTa                                         <  1:3234954/150‑1 (MQ=255)
                                                                                                            aGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTAc                                        <  1:4069890/150‑1 (MQ=255)
                                                                                                            aGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTAc                                        >  2:1779719/1‑150 (MQ=255)
                                                                                                              cGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACtt                                      <  1:914253/150‑1 (MQ=255)
                                                                                                                ttCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTGTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGc                                    >  2:3486204/1‑150 (MQ=255)
                                                                                                                 tCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCg                                   <  1:138733/150‑1 (MQ=255)
                                                                                                                    aaCGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGaa                                >  1:776041/1‑150 (MQ=255)
                                                                                                                    aaCGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGaa                                >  1:3543435/1‑150 (MQ=255)
                                                                                                                     aCGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGacc                               >  1:2170045/1‑148 (MQ=255)
                                                                                                                      cGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACg                              >  1:3148431/1‑150 (MQ=255)
                                                                                                                             cgtcgGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTc                       <  2:596984/150‑1 (MQ=255)
                                                                                                                             cgtcgGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTc                       <  1:2264041/150‑1 (MQ=255)
                                                                                                                              gtcgGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCa                      <  1:3199246/150‑1 (MQ=255)
                                                                                                                              gtcgGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCa                      <  2:2343653/150‑1 (MQ=255)
                                                                                                                               tcgGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCaa                     >  2:4230758/1‑150 (MQ=255)
                                                                                                                                cgGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCaaa                    >  1:684435/1‑150 (MQ=255)
                                                                                                                                 ggAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAg                   <  2:446448/150‑1 (MQ=255)
                                                                                                                                   aaGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGc                 >  2:2918048/1‑150 (MQ=255)
                                                                                                                                   aaGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGACGTCAAAGGc                 >  2:3538211/1‑150 (MQ=255)
                                                                                                                                       gTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGcagcc             >  1:1105580/1‑150 (MQ=255)
                                                                                                                                        ttCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGcagcca            <  2:3441375/150‑1 (MQ=255)
                                                                                                                                         tCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGcagccag           <  2:1012827/150‑1 (MQ=255)
                                                                                                                                         tCACATCAGATATGATCGCGTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACATTACTAGCGGAACGGGAAGTCAAAGGcagccag           >  1:2013328/1‑150 (MQ=255)
                                                                                                                                           acaTCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGACCGGGAAGTCAAAGGCAGCcagca         >  2:4092738/1‑150 (MQ=255)
                                                                                                                                            caTCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCcagcag        <  1:4454020/150‑1 (MQ=255)
                                                                                                                                             aTCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCATCCAGCaga       >  1:344476/1‑150 (MQ=255)
                                                                                                                                               cAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGACGTCAAAGGCAGCCAGCAGAgc     >  2:3514160/1‑150 (MQ=255)
                                                                                                                                               cAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAgc     <  1:3613248/150‑1 (MQ=255)
                                                                                                                                                agatATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAgcg    <  2:4155885/150‑1 (MQ=255)
                                                                                                                                                agatATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAgcg    >  1:3671952/1‑150 (MQ=255)
                                                                                                                                                  agaTGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGcgcg  >  1:4259410/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCG  >  CP009273/3440004‑3440299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGG  >  CP009273/3440009‑3440300
                                                                                                                                              |                                                                                                                                                     
ACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATCTGATCG                                                                                                                                                >  SRR19973083.4581100/1‑150 (MQ=60)
  CCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCT                                                                                                                                              <  SRR19973083.3809536/150‑1 (MQ=60)
  CCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCT                                                                                                                                              <  SRR19973083.413154/150‑1 (MQ=60)
    GAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCCGATATGATCGCTTT                                                                                                                                            >  SRR19973083.627893/1‑150 (MQ=60)
    GAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTT                                                                                                                                            <  SRR19973083.4386141/150‑1 (MQ=60)
    GAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGAAGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTGT                                                                                                                                            >  SRR19973083.628547/1‑150 (MQ=60)
       CTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAG                                                                                                                                         >  SRR19973083.5138770/1‑150 (MQ=60)
        TTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGT                                                                                                                                        >  SRR19973083.3258799/1‑150 (MQ=60)
          CCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTC                                                                                                                                      <  SRR19973083.1645849/150‑1 (MQ=60)
            GCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAG                                                                                                                                    >  SRR19973083.1966202/1‑150 (MQ=60)
               GGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGC                                                                                                                                 <  SRR19973083.4267091/150‑1 (MQ=60)
                   ACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTT                                                                                                                             >  SRR19973083.1977007/1‑150 (MQ=60)
                   ACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTT                                                                                                                             <  SRR19973083.3980708/150‑1 (MQ=60)
                     CATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCG                                                                                                                           >  SRR19973083.2543249/1‑150 (MQ=60)
                       TGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCG                                                                                                                         >  SRR19973083.4020430/1‑150 (MQ=60)
                       TGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCG                                                                                                                         <  SRR19973083.147135/150‑1 (MQ=60)
                        GCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGA                                                                                                                        >  SRR19973083.1646537/1‑150 (MQ=60)
                          GGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAG                                                                                                                      <  SRR19973083.4736496/150‑1 (MQ=60)
                            ACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTA                                                                                                                    <  SRR19973083.1322138/150‑1 (MQ=60)
                            ACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTA                                                                                                                    >  SRR19973083.2902391/1‑150 (MQ=60)
                                   GTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCA                                                                                                             <  SRR19973083.4418712/150‑1 (MQ=60)
                                      TGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCT                                                                                                          <  SRR19973083.4187581/150‑1 (MQ=60)
                                       GACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTA                                                                                                         >  SRR19973083.3142120/1‑150 (MQ=60)
                                         CGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAA                                                                                                       >  SRR19973083.4513568/1‑150 (MQ=60)
                                           GCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGC                                                                                                     <  SRR19973083.1385125/150‑1 (MQ=60)
                                           GCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGC                                                                                                     <  SRR19973083.1315751/150‑1 (MQ=60)
                                           GCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGC                                                                                                     <  SRR19973083.3922541/150‑1 (MQ=60)
                                           GCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGC                                                                                                     <  SRR19973083.2776405/150‑1 (MQ=60)
                                           GCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGC                                                                                                     <  SRR19973083.3992415/150‑1 (MQ=60)
                                               CGTTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACG                                                                                                 <  SRR19973083.3146542/150‑1 (MQ=60)
                                                   TACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTT                                                                                             >  SRR19973083.3979410/1‑150 (MQ=60)
                                                   TACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTT                                                                                             >  SRR19973083.326044/1‑150 (MQ=60)
                                                   TACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTT                                                                                             >  SRR19973083.1697280/1‑150 (MQ=60)
                                                    ACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTT                                                                                            >  SRR19973083.590366/1‑150 (MQ=60)
                                                    ACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTT                                                                                            >  SRR19973083.590377/1‑150 (MQ=60)
                                                            GAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGATTTACTTCGC                                                                                    >  SRR19973083.2649655/1‑150 (MQ=60)
                                                             AGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCG                                                                                   >  SRR19973083.3223072/1‑150 (MQ=60)
                                                              GACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGT                                                                                  <  SRR19973083.2873347/150‑1 (MQ=60)
                                                              GACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGT                                                                                  <  SRR19973083.2873359/150‑1 (MQ=60)
                                                              GACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGT                                                                                  <  SRR19973083.2873406/150‑1 (MQ=60)
                                                              GACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGT                                                                                  <  SRR19973083.2552799/150‑1 (MQ=60)
                                                              GACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGT                                                                                  <  SRR19973083.2987986/150‑1 (MQ=60)
                                                              GACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGT                                                                                  >  SRR19973083.5061220/1‑150 (MQ=60)
                                                                  GGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTT                                                                              >  SRR19973083.364992/1‑150 (MQ=60)
                                                                     TGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCA                                                                           <  SRR19973083.320500/150‑1 (MQ=60)
                                                                      GGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCAT                                                                          <  SRR19973083.4086358/150‑1 (MQ=60)
                                                                       GAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATT                                                                         >  SRR19973083.3504335/1‑150 (MQ=60)
                                                                       GAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATT                                                                         >  SRR19973083.3504497/1‑150 (MQ=60)
                                                                       GAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATT                                                                         >  SRR19973083.891921/1‑150 (MQ=60)
                                                                          TCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGAT                                                                      <  SRR19973083.788341/150‑1 (MQ=60)
                                                                          TCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGAT                                                                      <  SRR19973083.930514/150‑1 (MQ=60)
                                                                          TCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGAT                                                                      <  SRR19973083.3180602/150‑1 (MQ=60)
                                                                            ACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTG                                                                    <  SRR19973083.4358586/150‑1 (MQ=60)
                                                                            ACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTG                                                                    <  SRR19973083.3515391/150‑1 (MQ=60)
                                                                             CGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGC                                                                   <  SRR19973083.153800/150‑1 (MQ=60)
                                                                                GGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTA                                                                <  SRR19973083.2766301/150‑1 (MQ=60)
                                                                                 GCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAG                                                               <  SRR19973083.4115598/150‑1 (MQ=60)
                                                                                 GCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAG                                                               <  SRR19973083.8774/150‑1 (MQ=60)
                                                                                 GCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAG                                                               <  SRR19973083.2423756/150‑1 (MQ=60)
                                                                                  CAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGC                                                              <  SRR19973083.4526006/150‑1 (MQ=60)
                                                                                  CAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGC                                                              <  SRR19973083.1264041/150‑1 (MQ=60)
                                                                                  CAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTGTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGC                                                              <  SRR19973083.621195/150‑1 (MQ=60)
                                                                                    GAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCA                                                            >  SRR19973083.3789715/1‑150 (MQ=60)
                                                                                    GAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCA                                                            >  SRR19973083.3789720/1‑150 (MQ=60)
                                                                                      GTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATT                                                          <  SRR19973083.261301/150‑1 (MQ=60)
                                                                                         TGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAG                                                       >  SRR19973083.4035182/1‑150 (MQ=60)
                                                                                          GCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGT                                                      >  SRR19973083.3389188/1‑150 (MQ=60)
                                                                                          GCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGT                                                      >  SRR19973083.1299282/1‑150 (MQ=60)
                                                                                          GCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGT                                                      >  SRR19973083.486307/1‑150 (MQ=60)
                                                                                            AGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTAAGCCATTTAGTAA                                                    >  SRR19973083.4720584/1‑150 (MQ=60)
                                                                                              CTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACC                                                  >  SRR19973083.3784824/1‑150 (MQ=60)
                                                                                               TTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCC                                                 <  SRR19973083.3481165/150‑1 (MQ=60)
                                                                                                     ACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTT                                           <  SRR19973083.105441/150‑1 (MQ=60)
                                                                                                      CAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTA                                          <  SRR19973083.3250269/150‑1 (MQ=60)
                                                                                                       AGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTAC                                         <  SRR19973083.4087954/150‑1 (MQ=60)
                                                                                                         CGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTT                                       >  SRR19973083.3501033/1‑150 (MQ=60)
                                                                                                         CGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTT                                       <  SRR19973083.918643/150‑1 (MQ=60)
                                                                                                         CGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTT                                       >  SRR19973083.2066878/1‑150 (MQ=60)
                                                                                                          GTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTG                                      >  SRR19973083.1290445/1‑150 (MQ=60)
                                                                                                          GTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTG                                      >  SRR19973083.1290412/1‑150 (MQ=60)
                                                                                                            TCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCG                                    <  SRR19973083.139587/150‑1 (MQ=60)
                                                                                                               AACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAA                                 >  SRR19973083.3559704/1‑150 (MQ=60)
                                                                                                               AACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAA                                 <  SRR19973083.3625051/150‑1 (MQ=60)
                                                                                                               AACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAA                                 >  SRR19973083.779822/1‑150 (MQ=60)
                                                                                                               AACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAA                                 >  SRR19973083.779444/1‑150 (MQ=60)
                                                                                                                ACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGACC                                >  SRR19973083.2180922/1‑150 (MQ=60)
                                                                                                                 CGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACG                               >  SRR19973083.3163470/1‑150 (MQ=60)
                                                                                                                    TCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGA                            <  SRR19973083.4565841/150‑1 (MQ=60)
                                                                                                                     CTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAN                           <  SRR19973083.1095351/150‑1 (MQ=60)
                                                                                                                      TTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAG                          <  SRR19973083.2233750/150‑1 (MQ=60)
                                                                                                                        CGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTC                        <  SRR19973083.2275602/150‑1 (MQ=60)
                                                                                                                         GTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCA                       <  SRR19973083.3214449/150‑1 (MQ=60)
                                                                                                                          TCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAA                      >  SRR19973083.1242353/1‑150 (MQ=60)
                                                                                                                           CGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAA                     >  SRR19973083.687853/1‑150 (MQ=60)
                                                                                                                             GAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGG                   >  SRR19973083.300636/1‑150 (MQ=60)
                                                                                                                                GCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGACGTCAAAGGCAG                >  SRR19973083.3363315/1‑150 (MQ=60)
                                                                                                                                GCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAG                >  SRR19973083.746543/1‑150 (MQ=60)
                                                                                                                                  GTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCC              >  SRR19973083.1111134/1‑150 (MQ=60)
                                                                                                                                  GTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCC              <  SRR19973083.4942406/150‑1 (MQ=60)
                                                                                                                                    TCACATCAGATATGATCGCGTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACATTACTAGCGGAACGGGAAGTCAAAGGCAGCCAG            >  SRR19973083.2023619/1‑150 (MQ=60)
                                                                                                                                       CATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAG         <  SRR19973083.4473379/150‑1 (MQ=60)
                                                                                                                                        ATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCATCCAGCAGA        >  SRR19973083.2884372/1‑150 (MQ=60)
                                                                                                                                        ATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCATCCAGCAGA        >  SRR19973083.346423/1‑150 (MQ=60)
                                                                                                                                        ATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCATCCAGCAGA        >  SRR19973083.346134/1‑150 (MQ=60)
                                                                                                                                          CAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGC      <  SRR19973083.3629936/150‑1 (MQ=60)
                                                                                                                                          CAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGC      <  SRR19973083.3629816/150‑1 (MQ=60)
                                                                                                                                          CAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGC      <  SRR19973083.3629795/150‑1 (MQ=60)
                                                                                                                                           AGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCG     >  SRR19973083.3688714/1‑150 (MQ=60)
                                                                                                                                           AGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCG     >  SRR19973083.3688721/1‑150 (MQ=60)
                                                                                                                                            GATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGC    >  SRR19973083.11673/1‑150 (MQ=60)
                                                                                                                                            GATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGC    >  SRR19973083.1776524/1‑150 (MQ=60)
                                                                                                                                             AGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCG   >  SRR19973083.4278011/1‑150 (MQ=60)
                                                                                                                                             AGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCG   >  SRR19973083.4278212/1‑150 (MQ=60)
                                                                                                                                              TATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGG  >  SRR19973083.893448/1‑150 (MQ=60)
                                                                                                                                              |                                                                                                                                                     
ACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGG  >  CP009273/3440009‑3440300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑