Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,837,532 C→A 100% P70Q (CCG→CAG)  ade → cryptic adenine deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,837,5320CA99.1% 739.7 / ‑1.9 228P70Q (CCG→CAG) adecryptic adenine deaminase
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (110/116);  total (112/116)
Fisher's exact test for biased strand distribution p-value = 2.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 228 total aligned reads displayed.

CCGTTTCCCGTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGA  >  CP009273/3837388‑3837676
                                                                                                                                                |                                                                                                                                                
ccGTTTCCCGTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGc                                                                                                                                               <  1:2491635/148‑1 (MQ=255)
 cGTTTCCCGTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCt                                                                                                                                              <  2:1773364/148‑1 (MQ=255)
   tttCCCGTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCttt                                                                                                                                            >  2:2112402/1‑148 (MQ=255)
       ccGTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTTGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAg                                                                                                                                        >  2:1148676/1‑148 (MQ=255)
          tGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCgg                                                                                                                                     >  1:2381284/1‑148 (MQ=255)
               aCGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGa                                                                                                                                >  2:461675/1‑148 (MQ=255)
               aCGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGa                                                                                                                                >  2:1551315/1‑148 (MQ=255)
                  cTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATTGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGc                                                                                                                             >  1:900179/1‑148 (MQ=255)
                  cTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGc                                                                                                                             >  2:2372041/1‑148 (MQ=255)
                  cTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGc                                                                                                                             >  2:1495321/1‑148 (MQ=255)
                   tGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCt                                                                                                                            <  1:3411132/148‑1 (MQ=255)
                   tGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCt                                                                                                                            <  1:2049393/148‑1 (MQ=255)
                   tGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCt                                                                                                                            <  2:1812680/148‑1 (MQ=255)
                     ttGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTcg                                                                                                                          <  2:2538884/148‑1 (MQ=255)
                      tGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGCGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTcgc                                                                                                                         >  1:413077/1‑148 (MQ=255)
                            ttatattatTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCa                                                                                                                   <  1:2247384/148‑1 (MQ=255)
                            ttatattatTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCa                                                                                                                   <  1:2615020/148‑1 (MQ=255)
                                 ttattGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAAcggc                                                                                                              <  1:115091/148‑1 (MQ=255)
                                     tGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTg                                                                                                          <  1:532870/148‑1 (MQ=255)
                                      gATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGc                                                                                                         <  2:2100951/148‑1 (MQ=255)
                                       aTAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGcc                                                                                                        >  2:2432931/1‑148 (MQ=255)
                                          aTGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAgg                                                                                                     >  2:911988/1‑148 (MQ=255)
                                          aTGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAgg                                                                                                     >  2:890435/1‑148 (MQ=255)
                                            gTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGt                                                                                                   <  1:695351/148‑1 (MQ=255)
                                            gTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGt                                                                                                   >  2:1933079/1‑148 (MQ=255)
                                            gTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGt                                                                                                   >  2:3158774/1‑148 (MQ=255)
                                                   ttCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGa                                                                                            <  2:964592/148‑1 (MQ=255)
                                                   ttCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGa                                                                                            >  1:2695828/1‑148 (MQ=255)
                                                       cGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCt                                                                                        <  2:404794/148‑1 (MQ=255)
                                                       cGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCt                                                                                        <  1:543691/148‑1 (MQ=255)
                                                          ccTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCAc                                                                                     >  2:1375098/1‑148 (MQ=255)
                                                             gATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTGACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTg                                                                                  <  2:1898483/148‑1 (MQ=255)
                                                              aTCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGc                                                                                 <  1:3487034/148‑1 (MQ=255)
                                                              aTCAATGGCGGAGAAATTTACTGCCCAATTTTGATTAAAGGACGTTACATTGACGGTGTTGGCGCATAATACACTGATGATCAGGCTTTGCAGCTGATAGATGCTCGCGGAGCAACTGCGGTCACAGGGTTTATTGATGCTCACCTGc                                                                                 >  2:1498474/1‑148 (MQ=255)
                                                               tCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTAAAATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTGTGCTGCGGATTGATGCTCGCGGCGCACCGGCGGTGCCAGGGTTTATTGATGCTCACCTGCa                                                                                >  2:1487120/1‑148 (MQ=255)
                                                                cAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCat                                                                               <  1:2707505/148‑1 (MQ=255)
                                                                  aTGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCatat                                                                             <  2:1422114/148‑1 (MQ=255)
                                                                  aTGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCatat                                                                             <  1:1867043/148‑1 (MQ=255)
                                                                     gCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGa                                                                          >  1:1096052/1‑148 (MQ=255)
                                                                     gCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGa                                                                          >  1:2781943/1‑148 (MQ=255)
                                                                        gagaAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATc                                                                       >  2:322708/1‑148 (MQ=255)
                                                                        gagaAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATc                                                                       >  1:730226/1‑148 (MQ=255)
                                                                         agaAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATcc                                                                      >  2:258199/1‑148 (MQ=255)
                                                                           aaaTTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAg                                                                    >  1:3449127/1‑148 (MQ=255)
                                                                           aaaTTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCAGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAg                                                                    <  2:2769438/148‑1 (MQ=255)
                                                                            aaTTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGc                                                                   <  2:64594/148‑1 (MQ=255)
                                                                             aTTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATAAACTGATGCTCAGGCTTTGCAGCGGATTGATGCTAGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCa                                                                  >  1:1935338/1‑148 (MQ=255)
                                                                               ttCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCatg                                                                <  1:2101589/148‑1 (MQ=255)
                                                                               gtCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCatg                                                                <  2:3430435/147‑1 (MQ=255)
                                                                                tCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCatga                                                               <  1:69085/148‑1 (MQ=255)
                                                                                tCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCatga                                                               <  1:1058797/148‑1 (MQ=255)
                                                                                  cGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCatgatg                                                             <  2:3120847/148‑1 (MQ=255)
                                                                                  cGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCatgatg                                                             <  1:95246/148‑1 (MQ=255)
                                                                                  cGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCatgatg                                                             >  2:2223472/1‑148 (MQ=255)
                                                                                     cccAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACg                                                          <  1:3159789/148‑1 (MQ=255)
                                                                                      ccAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGc                                                         <  1:2112402/148‑1 (MQ=255)
                                                                                        aaTTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCg                                                       <  1:3014834/148‑1 (MQ=255)
                                                                                        aaTTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCg                                                       >  2:233451/1‑148 (MQ=255)
                                                                                        aaTTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCg                                                       <  1:3014332/148‑1 (MQ=255)
                                                                                        aaTTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCg                                                       >  2:2702493/1‑148 (MQ=255)
                                                                                         aTTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCgg                                                      >  2:19470/1‑148 (MQ=255)
                                                                                           tgtgATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTc                                                    >  1:1422374/1‑148 (MQ=255)
                                                                                            gtgATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCa                                                   <  1:2210317/148‑1 (MQ=255)
                                                                                            gtgATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCa                                                   <  1:1033219/148‑1 (MQ=255)
                                                                                                ttAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCCGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACttt                                               >  2:1045660/1‑148 (MQ=255)
                                                                                                ttAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACttt                                               >  2:2971649/1‑148 (MQ=255)
                                                                                                 tAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACtttt                                              <  1:3263698/148‑1 (MQ=255)
                                                                                                 tAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACtttt                                              >  2:1274075/1‑148 (MQ=255)
                                                                                                  aaaGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTg                                             <  2:535505/148‑1 (MQ=255)
                                                                                                    aGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGaa                                           >  1:363995/1‑148 (MQ=255)
                                                                                                    aGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGaa                                           <  2:2846156/148‑1 (MQ=255)
                                                                                                        cGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCg                                       >  1:2662166/1‑148 (MQ=255)
                                                                                                        cGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCg                                       >  1:2894564/1‑148 (MQ=255)
                                                                                                        cGTTACATTCCCGGTGTTGGCGCAGAATACACTGATGCTAAGGCTTTGCAGCGTATTGATGCTCGCGGCGCAACGGCGGTGCCAGTGTTTATTGATGCTCACCTGCATAATGAATCCAGCATGATGACGCCGGTCACTTGTGAAACCg                                       >  1:2318125/1‑148 (MQ=255)
                                                                                                           tACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTa                                    >  2:2050817/1‑148 (MQ=255)
                                                                                                           tACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTa                                    <  1:19470/148‑1 (MQ=255)
                                                                                                            aCATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTAc                                   <  2:2910245/148‑1 (MQ=255)
                                                                                                               ttGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCt                                >  1:2585536/1‑148 (MQ=255)
                                                                                                                tGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTg                               <  1:1478184/148‑1 (MQ=255)
                                                                                                                tGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTg                               >  2:837113/1‑148 (MQ=255)
                                                                                                                tGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTg                               <  1:1533945/148‑1 (MQ=255)
                                                                                                                tGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTg                               >  1:1367612/1‑148 (MQ=255)
                                                                                                                     gtgtTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCcgcg                          <  1:1283633/148‑1 (MQ=255)
                                                                                                                         tGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGc                      <  2:2236886/148‑1 (MQ=255)
                                                                                                                         tGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGc                      <  2:2236674/148‑1 (MQ=255)
                                                                                                                          ggCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGcc                     <  1:3238457/148‑1 (MQ=255)
                                                                                                                             gcAGAATACACTGATGCTCAGGATTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTga                  <  1:1380734/148‑1 (MQ=255)
                                                                                                                              cAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTgac                 >  2:2017477/1‑148 (MQ=255)
                                                                                                                                gAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTgacga               <  1:2471150/148‑1 (MQ=255)
                                                                                                                                gAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTgacga               <  1:1628621/148‑1 (MQ=255)
                                                                                                                                 aaTACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTgacgac              >  1:2856592/1‑148 (MQ=255)
                                                                                                                                   tACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCg            <  2:1871311/148‑1 (MQ=255)
                                                                                                                                   tACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCg            <  2:95443/148‑1 (MQ=255)
                                                                                                                                     cacTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGtt          >  1:3319091/1‑148 (MQ=255)
                                                                                                                                       cTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTAt        >  1:2443079/1‑148 (MQ=255)
                                                                                                                                        tGATGCTGAGGCTTTGCAGCGGTTTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATTTTGAATCCAGCTTGTTGACGCCGGTCACTTTTGAATCCGCTACCCTTCCGCGCGGCCTGACGACCGTTAtt       <  1:3404401/148‑1 (MQ=255)
                                                                                                                                        tGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTAtt       <  2:3097217/148‑1 (MQ=255)
                                                                                                                                         gATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTAttt      >  2:3400614/1‑148 (MQ=255)
                                                                                                                                            gCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCg   <  1:1844179/148‑1 (MQ=255)
                                                                                                                                             cTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGa  >  2:72388/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CCGTTTCCCGTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGA  >  CP009273/3837388‑3837676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCCGTTTCCCGTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACCC  >  CP009273/3837386‑3837679
                                                                                                                                                  |                                                                                                                                                   
AGCCGTTTCCCGTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGC                                                                                                                                                  <  SRR19973065.1518331/150‑1 (MQ=60)
  CCGTTTCCCGTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTT                                                                                                                                                <  SRR19973065.2507317/150‑1 (MQ=60)
  CCGTTTCCCGTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTT                                                                                                                                                >  SRR19973065.35210/1‑150 (MQ=60)
   CGTTTCCCGTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTT                                                                                                                                               >  SRR19973065.1861752/1‑150 (MQ=60)
          CGTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGG                                                                                                                                        >  SRR19973065.2396191/1‑150 (MQ=60)
             GGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATT                                                                                                                                     <  SRR19973065.2761165/150‑1 (MQ=60)
                GACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGAT                                                                                                                                  <  SRR19973065.1716933/150‑1 (MQ=60)
                GACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGAT                                                                                                                                  <  SRR19973065.2052079/150‑1 (MQ=60)
                 ACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATG                                                                                                                                 <  SRR19973065.1370625/150‑1 (MQ=60)
                  CGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATTGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGC                                                                                                                                >  SRR19973065.905950/1‑150 (MQ=60)
                  CGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGC                                                                                                                                >  SRR19973065.1429429/1‑150 (MQ=60)
                     TGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCG                                                                                                                             <  SRR19973065.2270972/150‑1 (MQ=60)
                     TGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCG                                                                                                                             <  SRR19973065.2062489/150‑1 (MQ=60)
                     TGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCG                                                                                                                             <  SRR19973065.3431971/150‑1 (MQ=60)
                      GTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGCGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGC                                                                                                                            >  SRR19973065.415968/1‑150 (MQ=60)
                             ATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAA                                                                                                                     >  SRR19973065.3431423/1‑150 (MQ=60)
                             ATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAA                                                                                                                     >  SRR19973065.3431432/1‑150 (MQ=60)
                             ATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAA                                                                                                                     >  SRR19973065.3531298/1‑150 (MQ=60)
                              TTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAAC                                                                                                                    <  SRR19973065.2631227/150‑1 (MQ=60)
                              TTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAAC                                                                                                                    >  SRR19973065.3199119/1‑150 (MQ=60)
                              TTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAAC                                                                                                                    <  SRR19973065.2261641/150‑1 (MQ=60)
                                 TATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGC                                                                                                                 >  SRR19973065.2411795/1‑150 (MQ=60)
                                  ATTATTGATCATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCG                                                                                                                >  SRR19973065.77842/1‑150 (MQ=60)
                                   TTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGG                                                                                                               <  SRR19973065.116109/150‑1 (MQ=60)
                                    TATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGT                                                                                                              >  SRR19973065.2956603/1‑150 (MQ=60)
                                     ATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTG                                                                                                             >  SRR19973065.1230029/1‑150 (MQ=60)
                                     ATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTG                                                                                                             <  SRR19973065.1508682/150‑1 (MQ=60)
                                     ATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTG                                                                                                             >  SRR19973065.2989259/1‑150 (MQ=60)
                                       TGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCC                                                                                                           <  SRR19973065.536350/150‑1 (MQ=60)
                                         ATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAG                                                                                                         <  SRR19973065.198613/150‑1 (MQ=60)
                                         ATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAG                                                                                                         <  SRR19973065.2881081/150‑1 (MQ=60)
                                             TGTCTCTATTCTAGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTT                                                                                                     <  SRR19973065.2241715/150‑1 (MQ=60)
                                              GTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTT                                                                                                    <  SRR19973065.699978/150‑1 (MQ=60)
                                              GTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTT                                                                                                    <  SRR19973065.698212/150‑1 (MQ=60)
                                                CTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTAT                                                                                                  >  SRR19973065.1761862/1‑150 (MQ=60)
                                                  CTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTG                                                                                                <  SRR19973065.2616286/150‑1 (MQ=60)
                                                  CTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTG                                                                                                <  SRR19973065.1451604/150‑1 (MQ=60)
                                                   TATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGA                                                                                               >  SRR19973065.2712572/1‑150 (MQ=60)
                                                   TATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGA                                                                                               >  SRR19973065.2713362/1‑150 (MQ=60)
                                                   TATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGA                                                                                               >  SRR19973065.2713380/1‑150 (MQ=60)
                                                     TTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATG                                                                                             <  SRR19973065.2184010/150‑1 (MQ=60)
                                                         CGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCA                                                                                         <  SRR19973065.547149/150‑1 (MQ=60)
                                                         CGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCA                                                                                         <  SRR19973065.547212/150‑1 (MQ=60)
                                                           ACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGGGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACC                                                                                       >  SRR19973065.343529/1‑150 (MQ=60)
                                                               GATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCA                                                                                   >  SRR19973065.1605301/1‑150 (MQ=60)
                                                                ATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCAT                                                                                  <  SRR19973065.3508242/150‑1 (MQ=60)
                                                                 TCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATA                                                                                 <  SRR19973065.938602/150‑1 (MQ=60)
                                                                  CAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATAT                                                                                <  SRR19973065.2724336/150‑1 (MQ=60)
                                                                    ATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTG                                                                              <  SRR19973065.1879332/150‑1 (MQ=60)
                                                                     TGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGA                                                                             >  SRR19973065.2799201/1‑150 (MQ=60)
                                                                     TGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGA                                                                             >  SRR19973065.1103264/1‑150 (MQ=60)
                                                                       GCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAAT                                                                           <  SRR19973065.3287865/150‑1 (MQ=60)
                                                                        CGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATC                                                                          >  SRR19973065.1924437/1‑150 (MQ=60)
                                                                        CGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATC                                                                          >  SRR19973065.735120/1‑150 (MQ=60)
                                                                          GAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCA                                                                        <  SRR19973065.2484253/150‑1 (MQ=60)
                                                                          GAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCA                                                                        <  SRR19973065.714053/150‑1 (MQ=60)
                                                                           AGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAG                                                                       >  SRR19973065.1067718/1‑150 (MQ=60)
                                                                           AGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAG                                                                       <  SRR19973065.404360/150‑1 (MQ=60)
                                                                           AGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAG                                                                       >  SRR19973065.3470157/1‑150 (MQ=60)
                                                                             AAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATAAACTGATGCTCAGGCTTTGCAGCGGATTGATGCTAGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCA                                                                     >  SRR19973065.1948005/1‑150 (MQ=60)
                                                                                 TTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGAT                                                                 <  SRR19973065.2115104/150‑1 (MQ=60)
                                                                                 TTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGAT                                                                 <  SRR19973065.1384171/150‑1 (MQ=60)
                                                                                 TTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGAT                                                                 <  SRR19973065.2115048/150‑1 (MQ=60)
                                                                                  TCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATG                                                                <  SRR19973065.2701990/150‑1 (MQ=60)
                                                                                  TCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATG                                                                <  SRR19973065.69818/150‑1 (MQ=60)
                                                                                  TCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATG                                                                <  SRR19973065.1065816/150‑1 (MQ=60)
                                                                                    CGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGAC                                                              <  SRR19973065.96141/150‑1 (MQ=60)
                                                                                       CCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCC                                                           <  SRR19973065.3178936/150‑1 (MQ=60)
                                                                                        CCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCG                                                          <  SRR19973065.2125997/150‑1 (MQ=60)
                                                                                        CCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCG                                                          <  SRR19973065.2634370/150‑1 (MQ=60)
                                                                                        CCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCG                                                          <  SRR19973065.2125980/150‑1 (MQ=60)
                                                                                         CAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGG                                                         >  SRR19973065.2866389/1‑150 (MQ=60)
                                                                                          AATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGT                                                        <  SRR19973065.239183/150‑1 (MQ=60)
                                                                                          AATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGT                                                        >  SRR19973065.3348617/1‑150 (MQ=60)
                                                                                          AATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGT                                                        <  SRR19973065.3032791/150‑1 (MQ=60)
                                                                                          AATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGT                                                        <  SRR19973065.3032792/150‑1 (MQ=60)
                                                                                          AATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGT                                                        <  SRR19973065.3033298/150‑1 (MQ=60)
                                                                                           ATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTC                                                       >  SRR19973065.1456351/1‑150 (MQ=60)
                                                                                           ATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTC                                                       >  SRR19973065.1431649/1‑150 (MQ=60)
                                                                                              GTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACT                                                    <  SRR19973065.1040058/150‑1 (MQ=60)
                                                                                              GTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACT                                                    <  SRR19973065.2224444/150‑1 (MQ=60)
                                                                                              GTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACT                                                    <  SRR19973065.2224436/150‑1 (MQ=60)
                                                                                                 ATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTT                                                 >  SRR19973065.2505944/1‑150 (MQ=60)
                                                                                                   TAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGA                                               <  SRR19973065.3283479/150‑1 (MQ=60)
                                                                                                    AAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAG                                              >  SRR19973065.1143126/1‑150 (MQ=60)
                                                                                                    AAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAA                                              >  SRR19973065.366541/1‑150 (MQ=60)
                                                                                                    AAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAA                                              <  SRR19973065.1062933/150‑1 (MQ=60)
                                                                                                      AGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAAC                                            <  SRR19973065.446927/150‑1 (MQ=60)
                                                                                                      AGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAAC                                            >  SRR19973065.979592/1‑150 (MQ=60)
                                                                                                        GACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCG                                          >  SRR19973065.2912240/1‑150 (MQ=60)
                                                                                                        GACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCG                                          >  SRR19973065.2678637/1‑150 (MQ=60)
                                                                                                        GACGTTACATTCCCGGTGTTGGCGCAGAATACACTGATGCTAAGGCTTTGCAGCGTATTGATGCTCGCGGCGCAACGGCGGTGCCAGTGTTTATTGATGCTCACCTGCATAATGAATCCAGCATGATGACGCCGGTCACTTGTGAAACCG                                          >  SRR19973065.2332655/1‑150 (MQ=60)
                                                                                                            TTACATTGCCGGTGTTGGCGCAGAATACTCTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGTGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTAC                                      <  SRR19973065.3530217/150‑1 (MQ=60)
                                                                                                             TACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACC                                     <  SRR19973065.642729/150‑1 (MQ=60)
                                                                                                             TACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACC                                     <  SRR19973065.19739/150‑1 (MQ=60)
                                                                                                               CATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCT                                   >  SRR19973065.2601641/1‑150 (MQ=60)
                                                                                                                ATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTG                                  >  SRR19973065.1376648/1‑150 (MQ=60)
                                                                                                                  TGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCC                                <  SRR19973065.1487635/150‑1 (MQ=60)
                                                                                                                  TGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCC                                <  SRR19973065.1544059/150‑1 (MQ=60)
                                                                                                                  TGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCC                                >  SRR19973065.1952075/1‑150 (MQ=60)
                                                                                                                      GGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGC                            >  SRR19973065.2552383/1‑150 (MQ=60)
                                                                                                                      GGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGC                            >  SRR19973065.2552365/1‑150 (MQ=60)
                                                                                                                       GTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCG                           <  SRR19973065.1292101/150‑1 (MQ=60)
                                                                                                                            GGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTG                      <  SRR19973065.3257960/150‑1 (MQ=60)
                                                                                                                            GGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTG                      <  SRR19973065.3257983/150‑1 (MQ=60)
                                                                                                                               GCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACG                   <  SRR19973065.3146489/150‑1 (MQ=60)
                                                                                                                               GCAGAATACACTGATGCTCAGGATTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACG                   <  SRR19973065.1389824/150‑1 (MQ=60)
                                                                                                                                CAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGA                  >  SRR19973065.1161165/1‑150 (MQ=60)
                                                                                                                                 AGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGAC                 >  SRR19973065.869906/1‑150 (MQ=60)
                                                                                                                                 AGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGAC                 >  SRR19973065.2874142/1‑150 (MQ=60)
                                                                                                                                  GAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACC                <  SRR19973065.2486710/150‑1 (MQ=60)
                                                                                                                                  GAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACC                <  SRR19973065.1639537/150‑1 (MQ=60)
                                                                                                                                    ATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGT              <  SRR19973065.3217238/150‑1 (MQ=60)
                                                                                                                                     TACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTT             >  SRR19973065.3339338/1‑150 (MQ=60)
                                                                                                                                       CACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTAT           >  SRR19973065.2458475/1‑150 (MQ=60)
                                                                                                                                       CACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTAT           >  SRR19973065.2458476/1‑150 (MQ=60)
                                                                                                                                          TGATGCTGAGGCTTTGCAGCGGTTTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATTTTGAATCCAGCTTGTTGACGCCGGTCACTTTTGAATCCGCTACCCTTCCGCGCGGCCTGACGACCGTTATTTG        <  SRR19973065.3425205/150‑1 (MQ=60)
                                                                                                                                              GCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGAC    <  SRR19973065.1856340/150‑1 (MQ=60)
                                                                                                                                              GCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGAC    <  SRR19973065.618736/150‑1 (MQ=60)
                                                                                                                                                TCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACCC  >  SRR19973065.2059007/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                   
AGCCGTTTCCCGTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACCC  >  CP009273/3837386‑3837679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑