Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,863,438 G→T 100% M234I (ATG→ATT)  cbrA → colicin M resistance protein; FAD‑binding protein, putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,863,4380GT99.5% 602.8 / ‑4.2 186M234I (ATG→ATT) cbrAcolicin M resistance protein; FAD‑binding protein, putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (95/90);  total (96/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 186 total aligned reads displayed.

GTGCCGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCAGCTC  >  CP009273/3863295‑3863581
                                                                                                                                               |                                                                                                                                               
gTGCCGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAgcg                                                                                                                                             >  1:2973609/1‑148 (MQ=255)
  gCCGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGcc                                                                                                                                           <  1:1287002/148‑1 (MQ=255)
  gCCGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGcc                                                                                                                                           <  1:1879082/148‑1 (MQ=255)
    cGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCtt                                                                                                                                         >  2:2374124/1‑148 (MQ=255)
     gTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCttt                                                                                                                                        <  2:850935/148‑1 (MQ=255)
         tACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGt                                                                                                                                    >  1:2199428/1‑148 (MQ=255)
           cTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTc                                                                                                                                  <  1:405509/148‑1 (MQ=255)
           cTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTc                                                                                                                                  <  1:247981/148‑1 (MQ=255)
            tCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTATCGCCTTTCAGTTcc                                                                                                                                 >  2:1557063/1‑148 (MQ=255)
            tCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTcc                                                                                                                                 >  1:1056349/1‑148 (MQ=255)
            tCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTcc                                                                                                                                 <  2:2210100/148‑1 (MQ=255)
            tCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTcc                                                                                                                                 >  1:513287/1‑148 (MQ=255)
            tCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTcc                                                                                                                                 >  1:2277614/1‑148 (MQ=255)
              cTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAg                                                                                                                               <  2:3084969/148‑1 (MQ=255)
                   tCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGACAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTgg                                                                                                                          >  2:2125216/1‑148 (MQ=255)
                   tCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTgg                                                                                                                          >  2:3844/1‑148 (MQ=255)
                   tCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTgg                                                                                                                          >  1:2119014/1‑148 (MQ=255)
                      ttGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGAAGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTaa                                                                                                                       >  2:39578/1‑148 (MQ=255)
                       tGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAg                                                                                                                      <  1:2340004/148‑1 (MQ=255)
                        gATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGa                                                                                                                     <  1:1905521/148‑1 (MQ=255)
                        gATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGa                                                                                                                     <  1:2819079/148‑1 (MQ=255)
                         aTAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGAc                                                                                                                    >  1:1841231/1‑148 (MQ=255)
                         aTAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGAc                                                                                                                    >  1:62582/1‑148 (MQ=255)
                           aaTTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGTTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACgg                                                                                                                  <  1:1600226/148‑1 (MQ=255)
                              tCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGa                                                                                                               >  1:2012938/1‑148 (MQ=255)
                              tCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGa                                                                                                               >  2:552316/1‑148 (MQ=255)
                              tCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGa                                                                                                               >  1:2103916/1‑148 (MQ=255)
                                gATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGaaa                                                                                                             <  2:2433721/148‑1 (MQ=255)
                                 aTAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGaaaa                                                                                                            <  2:932448/148‑1 (MQ=255)
                                    actaactGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCg                                                                                                         <  2:1560257/148‑1 (MQ=255)
                                      taactGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGaa                                                                                                       >  2:2681481/1‑148 (MQ=255)
                                      taactGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGaa                                                                                                       >  2:1775673/1‑148 (MQ=255)
                                         ctGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGaaaaa                                                                                                    <  2:2946977/148‑1 (MQ=255)
                                                tCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACgg                                                                                             >  2:251564/1‑148 (MQ=255)
                                                tCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACgg                                                                                             >  2:1075359/1‑148 (MQ=255)
                                                   tGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGc                                                                                          >  1:2653966/1‑148 (MQ=255)
                                                   tGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGc                                                                                          <  1:1323183/148‑1 (MQ=255)
                                                    ggAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTgat                                                                                         <  1:952941/148‑3 (MQ=255)
                                                     gAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTg                                                                                        <  1:2162305/148‑1 (MQ=255)
                                                           cAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTccc                                                                                  >  2:1214807/1‑148 (MQ=255)
                                                              cAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTcg                                                                               >  2:1987729/1‑148 (MQ=255)
                                                               aaaGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTcgc                                                                              >  1:2652340/1‑148 (MQ=255)
                                                                aaGACGGTTATTTTATCTTTGGCTGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAAAGCACGGTGCTGTTTCCCTcgcg                                                                             >  1:2582289/1‑148 (MQ=255)
                                                                 aGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAGAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCgcgc                                                                            <  2:3442145/148‑1 (MQ=255)
                                                                  gACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCt                                                                           >  2:36417/1‑148 (MQ=255)
                                                                    cGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTgg                                                                         >  2:3345960/1‑148 (MQ=255)
                                                                    cGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAAATCACGGTGCTGTTTCCCTCGCGCTgg                                                                         >  2:3348026/1‑148 (MQ=255)
                                                                     ggTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGc                                                                        <  1:160569/148‑1 (MQ=255)
                                                                       ttATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAg                                                                      >  1:3472817/1‑148 (MQ=255)
                                                                       ttATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAg                                                                      >  1:3472798/1‑148 (MQ=255)
                                                                          ttttATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGAt                                                                   >  1:1455771/1‑148 (MQ=255)
                                                                           tttATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGAtt                                                                  >  1:1355302/1‑148 (MQ=255)
                                                                           tttATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGAtt                                                                  >  1:1355188/1‑148 (MQ=255)
                                                                            ttATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGAttt                                                                 >  2:1006984/1‑148 (MQ=255)
                                                                            ttATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGAttt                                                                 <  2:1339087/148‑1 (MQ=255)
                                                                            ttATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGAgtt                                                                 >  2:1001869/1‑148 (MQ=255)
                                                                             tATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGAtttt                                                                <  2:1408950/148‑1 (MQ=255)
                                                                              aTCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGAAGGATTTTg                                                               >  1:2182612/1‑148 (MQ=255)
                                                                               tCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGt                                                              >  1:1698017/1‑148 (MQ=255)
                                                                               tCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGt                                                              <  2:752087/148‑1 (MQ=255)
                                                                               tCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGt                                                              >  1:2407335/1‑148 (MQ=255)
                                                                               tCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGt                                                              <  2:3248752/148‑1 (MQ=255)
                                                                                cTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTc                                                             >  2:278120/1‑148 (MQ=255)
                                                                                       ggTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTa                                                      <  2:2382609/148‑1 (MQ=255)
                                                                                        gTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTaa                                                     >  1:3414903/1‑148 (MQ=255)
                                                                                        gTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTaa                                                     <  2:2622918/148‑1 (MQ=255)
                                                                                        gTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTaa                                                     >  2:2353020/1‑148 (MQ=255)
                                                                                              aTCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTACGGACaa                                               >  2:2311427/1‑148 (MQ=255)
                                                                                              aTCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACaa                                               >  2:2311467/1‑148 (MQ=255)
                                                                                              aTCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACaa                                               >  1:58468/1‑148 (MQ=255)
                                                                                                  aaTGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGcc                                           >  2:774863/1‑148 (MQ=255)
                                                                                                   aTGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCt                                          <  1:3230291/148‑1 (MQ=255)
                                                                                                    tGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCtt                                         >  2:3416294/1‑148 (MQ=255)
                                                                                                    tGAAAGACGCTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCtt                                         <  1:1825381/148‑1 (MQ=255)
                                                                                                     gAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCttt                                        >  2:555725/1‑148 (MQ=255)
                                                                                                      aaaGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTc                                       >  2:352534/1‑148 (MQ=255)
                                                                                                         gACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGa                                    <  2:2568497/148‑1 (MQ=255)
                                                                                                         gACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGa                                    <  1:1006984/148‑1 (MQ=255)
                                                                                                           cGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGAtt                                  <  2:933719/148‑1 (MQ=255)
                                                                                                                 gACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGac                            >  2:3314062/1‑147 (MQ=255)
                                                                                                                     cgTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAgcgg                        >  1:538098/1‑148 (MQ=255)
                                                                                                                       tttCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAgcggcg                      <  1:2420172/148‑1 (MQ=255)
                                                                                                                       tttCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAgcggcg                      <  1:354321/148‑1 (MQ=255)
                                                                                                                        ttCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGcggcgg                     >  2:3053870/1‑148 (MQ=255)
                                                                                                                         tCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCggg                    <  2:101499/148‑1 (MQ=255)
                                                                                                                         tCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCggg                    <  2:101819/148‑1 (MQ=255)
                                                                                                                         tCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCggg                    <  2:2810473/148‑1 (MQ=255)
                                                                                                                          cACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGa                   <  1:2881749/148‑1 (MQ=255)
                                                                                                                            cgacgCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGAtt                 <  1:3486649/148‑1 (MQ=255)
                                                                                                                             gacgCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGAttt                <  1:430888/148‑1 (MQ=255)
                                                                                                                              acgCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTa               <  2:3211203/148‑1 (MQ=255)
                                                                                                                               cgCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTAt              <  2:638721/148‑1 (MQ=255)
                                                                                                                                 cTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCa            <  1:1815162/148‑1 (MQ=255)
                                                                                                                                 cTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCa            >  2:2241041/1‑148 (MQ=255)
                                                                                                                                  tGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAg           >  2:442493/1‑148 (MQ=255)
                                                                                                                                   gAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAgc          >  1:842987/1‑148 (MQ=255)
                                                                                                                                     aaGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAgcgc        <  1:850746/148‑1 (MQ=255)
                                                                                                                                     aaGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAgcgc        >  1:2230802/1‑148 (MQ=255)
                                                                                                                                     aaGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGCCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAgcgc        <  1:1675767/148‑1 (MQ=255)
                                                                                                                                          aaaaTTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCAGCt   >  2:2074251/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                               
GTGCCGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCAGCTC  >  CP009273/3863295‑3863581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATCCGGTGCCGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCAGCTCGCTGG  >  CP009273/3863289‑3863586
                                                                                                                                                     |                                                                                                                                                    
CATCCGGTGCCGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATT                                                                                                                                                      <  SRR19973065.2448445/150‑1 (MQ=60)
CATCCGGTGCCGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATT                                                                                                                                                      <  SRR19973065.2448442/150‑1 (MQ=60)
CATCCGGTGCCGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATT                                                                                                                                                      <  SRR19973065.2448415/150‑1 (MQ=60)
CATCCGGTGCCGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATT                                                                                                                                                      <  SRR19973065.2448180/150‑1 (MQ=60)
 ATCCGGTGCCGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTA                                                                                                                                                     <  SRR19973065.990778/150‑1 (MQ=60)
    CGGTGCCGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCG                                                                                                                                                  >  SRR19973065.2991798/1‑150 (MQ=60)
        GCCGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTT                                                                                                                                              <  SRR19973065.1295488/150‑1 (MQ=60)
        GCCGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTT                                                                                                                                              <  SRR19973065.1891453/150‑1 (MQ=60)
             TCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGT                                                                                                                                         >  SRR19973065.2213509/1‑150 (MQ=60)
                ACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCC                                                                                                                                      >  SRR19973065.1063354/1‑150 (MQ=60)
                ACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCC                                                                                                                                      >  SRR19973065.516684/1‑150 (MQ=60)
                ACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCC                                                                                                                                      >  SRR19973065.2291983/1‑150 (MQ=60)
                 CTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCA                                                                                                                                     <  SRR19973065.2270636/150‑1 (MQ=60)
                 CTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCA                                                                                                                                     <  SRR19973065.249729/150‑1 (MQ=60)
                 CTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCA                                                                                                                                     <  SRR19973065.408349/150‑1 (MQ=60)
                       CATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGG                                                                                                                               >  SRR19973065.2132650/1‑150 (MQ=60)
                         TCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTA                                                                                                                             >  SRR19973065.3295891/1‑150 (MQ=60)
                           TTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAG                                                                                                                           <  SRR19973065.961363/150‑1 (MQ=60)
                           TTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAG                                                                                                                           <  SRR19973065.957765/150‑1 (MQ=60)
                             TGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGAC                                                                                                                         >  SRR19973065.1375574/1‑150 (MQ=60)
                             TGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGAC                                                                                                                         >  SRR19973065.1853378/1‑150 (MQ=60)
                             TGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGAC                                                                                                                         <  SRR19973065.2354612/150‑1 (MQ=60)
                             TGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGAC                                                                                                                         <  SRR19973065.580899/150‑1 (MQ=60)
                             TGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGAC                                                                                                                         >  SRR19973065.63246/1‑150 (MQ=60)
                             TGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGAC                                                                                                                         >  SRR19973065.63252/1‑150 (MQ=60)
                              GATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACG                                                                                                                        <  SRR19973065.2836495/150‑1 (MQ=60)
                              GATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACG                                                                                                                        <  SRR19973065.1918040/150‑1 (MQ=60)
                                 AATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGTCGGTTATTTTTTCTTTGGCGTTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTG                                                                                                                     <  SRR19973065.1610919/150‑1 (MQ=60)
                                  ATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGA                                                                                                                    >  SRR19973065.2025918/1‑150 (MQ=60)
                                  ATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGA                                                                                                                    >  SRR19973065.2117440/1‑150 (MQ=60)
                                       ATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGC                                                                                                               >  SRR19973065.2129283/1‑150 (MQ=60)
                                        TAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCG                                                                                                              >  SRR19973065.2180629/1‑150 (MQ=60)
                                        TAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCG                                                                                                              >  SRR19973065.359600/1‑150 (MQ=60)
                                               CTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATG                                                                                                       <  SRR19973065.1469473/150‑1 (MQ=60)
                                                    ATTCATGGTGTATCAGCAAAGACGGTTATTTTTTCTTGGGCGGTGCCTATCCATTTATAGTCTGTCAGACGCTTTTCACGTCGCTGAAAGAGAAAATTAGCGCCTTTGAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGG                                                                                                  <  SRR19973065.1321973/150‑1 (MQ=60)
                                                     TTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGT                                                                                                 <  SRR19973065.2137114/150‑1 (MQ=60)
                                                       CATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGC                                                                                               >  SRR19973065.2670358/1‑150 (MQ=60)
                                                        ATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCT                                                                                              <  SRR19973065.2346915/150‑1 (MQ=60)
                                                         TGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTG                                                                                             <  SRR19973065.1331931/150‑1 (MQ=60)
                                                         TGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTG                                                                                             <  SRR19973065.1331958/150‑1 (MQ=60)
                                                          GGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGATGT                                                                                            <  SRR19973065.959239/150‑1 (MQ=60)
                                                           GAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTT                                                                                           <  SRR19973065.2176202/150‑1 (MQ=60)
                                                                 CAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTC                                                                                     <  SRR19973065.855418/150‑1 (MQ=60)
                                                                   GCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGC                                                                                   >  SRR19973065.2668712/1‑150 (MQ=60)
                                                                    CAAAGACGGTTATTTTATCTTTGGCTGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAAAGCACGGTGCTGTTTCCCTCGCG                                                                                  >  SRR19973065.2598387/1‑150 (MQ=60)
                                                                      AAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCT                                                                                <  SRR19973065.1995700/150‑1 (MQ=60)
                                                                           GGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAG                                                                           <  SRR19973065.161891/150‑1 (MQ=60)
                                                                           GGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAG                                                                           >  SRR19973065.3493952/1‑150 (MQ=60)
                                                                           GGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAG                                                                           >  SRR19973065.3493943/1‑150 (MQ=60)
                                                                           GGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAG                                                                           >  SRR19973065.3493933/1‑150 (MQ=60)
                                                                            GTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGG                                                                          <  SRR19973065.2901066/150‑1 (MQ=60)
                                                                            GTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGG                                                                          <  SRR19973065.1872877/150‑1 (MQ=60)
                                                                              TATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGAT                                                                        >  SRR19973065.1465147/1‑150 (MQ=60)
                                                                               ATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATT                                                                       >  SRR19973065.1364169/1‑150 (MQ=60)
                                                                               ATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATT                                                                       >  SRR19973065.1364283/1‑150 (MQ=60)
                                                                               ATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATT                                                                       >  SRR19973065.1364266/1‑150 (MQ=60)
                                                                                  TTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTG                                                                    >  SRR19973065.415168/1‑150 (MQ=60)
                                                                                  TTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGAAGGATTTTG                                                                    >  SRR19973065.2196616/1‑150 (MQ=60)
                                                                                   TATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGT                                                                   >  SRR19973065.2422484/1‑150 (MQ=60)
                                                                                   TATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGT                                                                   >  SRR19973065.1709344/1‑150 (MQ=60)
                                                                                    ATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTC                                                                  >  SRR19973065.2584534/1‑150 (MQ=60)
                                                                                    ATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTC                                                                  >  SRR19973065.811486/1‑150 (MQ=60)
                                                                                         TGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGAGTTTGTCTGCGG                                                             >  SRR19973065.2452096/1‑150 (MQ=60)
                                                                                            CGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAA                                                          >  SRR19973065.3435768/1‑150 (MQ=60)
                                                                                                  CTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAA                                                    >  SRR19973065.2366671/1‑150 (MQ=60)
                                                                                                  CTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAA                                                    >  SRR19973065.59091/1‑150 (MQ=60)
                                                                                                   TATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAAC                                                   >  SRR19973065.2669945/1‑150 (MQ=60)
                                                                                                    ATACAATGAAAGACGGTCAGAAGATTTTCACGTAGCTGAAAGAGTAAATTAGAGCCTTTCAGTTCGAGTTTGGTAAGAAGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACG                                                  <  SRR19973065.243313/150‑1 (MQ=60)
                                                                                                         ATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTT                                             <  SRR19973065.3249691/150‑1 (MQ=60)
                                                                                                          TGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTC                                            <  SRR19973065.1837447/150‑1 (MQ=60)
                                                                                                          TGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTC                                            <  SRR19973065.48059/150‑1 (MQ=60)
                                                                                                          TGAAAGACGCTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTC                                            <  SRR19973065.1837403/150‑1 (MQ=60)
                                                                                                          TCAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTC                                            <  SRR19973065.1836281/150‑1 (MQ=60)
                                                                                                               GACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATT                                       <  SRR19973065.1013668/150‑1 (MQ=60)
                                                                                                                     CAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAA                                 <  SRR19973065.2892231/150‑1 (MQ=60)
                                                                                                                      AGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAG                                <  SRR19973065.3188627/150‑1 (MQ=60)
                                                                                                                         CGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGG                             >  SRR19973065.541600/1‑150 (MQ=60)
                                                                                                                             TTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGG                         <  SRR19973065.2435432/150‑1 (MQ=60)
                                                                                                                             TTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGG                         <  SRR19973065.356816/150‑1 (MQ=60)
                                                                                                                             TTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGG                         <  SRR19973065.355982/150‑1 (MQ=60)
                                                                                                                              TTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGA                        <  SRR19973065.1201714/150‑1 (MQ=60)
                                                                                                                                CACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATT                      <  SRR19973065.2899385/150‑1 (MQ=60)
                                                                                                                                  CGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTA                    <  SRR19973065.3507855/150‑1 (MQ=60)
                                                                                                                                  CGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTA                    <  SRR19973065.3507854/150‑1 (MQ=60)
                                                                                                                                   GACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTAT                   <  SRR19973065.433883/150‑1 (MQ=60)
                                                                                                                                      GCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAG                <  SRR19973065.2197450/150‑1 (MQ=60)
                                                                                                                                       CTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGC               >  SRR19973065.1312853/1‑150 (MQ=60)
                                                                                                                                       CTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGC               <  SRR19973065.1827112/150‑1 (MQ=60)
                                                                                                                                       CTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGC               <  SRR19973065.1827101/150‑1 (MQ=60)
                                                                                                                                       CTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGC               >  SRR19973065.848465/1‑150 (MQ=60)
                                                                                                                                         GAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGC             >  SRR19973065.2244999/1‑150 (MQ=60)
                                                                                                                                          AAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCC            <  SRR19973065.1706866/150‑1 (MQ=60)
                                                                                                                                           AAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCA           <  SRR19973065.856254/150‑1 (MQ=60)
                                                                                                                                           AAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGCCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCA           <  SRR19973065.1686980/150‑1 (MQ=60)
                                                                                                                                              AGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCAGCT        >  SRR19973065.2686105/1‑150 (MQ=60)
                                                                                                                                               GAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCAGCTC       >  SRR19973065.1647249/1‑150 (MQ=60)
                                                                                                                                                AAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCAGCTCG      <  SRR19973065.2047885/150‑1 (MQ=60)
                                                                                                                                                   ATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCAGCTCGCTG   >  SRR19973065.2217204/1‑150 (MQ=60)
                                                                                                                                                   ATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCAGCTCGCTG   >  SRR19973065.1702149/1‑150 (MQ=60)
                                                                                                                                                   ATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCAGCTCGCTG   >  SRR19973065.9054/1‑150 (MQ=60)
                                                                                                                                                    TTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCAGCTCGCTGG  <  SRR19973065.1556828/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CATCCGGTGCCGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCAGCTCGCTGG  >  CP009273/3863289‑3863586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑