Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,863,438 G→T 100% M234I (ATG→ATT)  cbrA → colicin M resistance protein; FAD‑binding protein, putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,863,4380GT99.1% 723.0 / ‑2.0 223M234I (ATG→ATT) cbrAcolicin M resistance protein; FAD‑binding protein, putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (128/93);  total (130/93)
Fisher's exact test for biased strand distribution p-value = 5.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 223 total aligned reads displayed.

TGCCGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCAGCTCG  >  CP009273/3863296‑3863582
                                                                                                                                              |                                                                                                                                                
  ccGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCt                                                                                                                                           <  1:1240297/148‑1 (MQ=255)
    gTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCttt                                                                                                                                         <  1:2841465/148‑1 (MQ=255)
     ttCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTc                                                                                                                                        >  2:3244684/1‑148 (MQ=255)
     ttCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTc                                                                                                                                        >  2:3244639/1‑148 (MQ=255)
          cTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTc                                                                                                                                   <  2:3526347/148‑1 (MQ=255)
              tGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGt                                                                                                                               <  1:2937123/148‑1 (MQ=255)
               gCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGtt                                                                                                                              <  1:1275854/148‑1 (MQ=255)
                 aTCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTg                                                                                                                            <  1:2504986/148‑1 (MQ=255)
                 aTCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTg                                                                                                                            <  2:1913002/148‑1 (MQ=255)
                   cTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGt                                                                                                                          >  2:3165908/1‑148 (MQ=255)
                   cTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGt                                                                                                                          <  2:421610/148‑1 (MQ=255)
                   cTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGt                                                                                                                          >  1:2328725/1‑148 (MQ=255)
                     ttGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTaa                                                                                                                        <  2:3443763/148‑1 (MQ=255)
                      tGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAg                                                                                                                       >  2:3391750/1‑148 (MQ=255)
                        aTAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGAc                                                                                                                     <  2:2562294/148‑1 (MQ=255)
                        aTAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGAc                                                                                                                     >  2:1187319/1‑148 (MQ=255)
                          aaTTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACgg                                                                                                                   >  2:2011839/1‑148 (MQ=255)
                            ttCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTg                                                                                                                 <  1:3301232/148‑1 (MQ=255)
                            ttCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTg                                                                                                                 >  1:2740314/1‑148 (MQ=255)
                            ttCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTg                                                                                                                 >  1:1137072/1‑148 (MQ=255)
                                aTAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGaaaa                                                                                                             >  2:160323/1‑148 (MQ=255)
                                aTAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGaaaa                                                                                                             >  2:2050821/1‑148 (MQ=255)
                                 taactaacTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAg                                                                                                            <  2:3204726/148‑1 (MQ=255)
                                    ctaactGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGa                                                                                                         >  2:818949/1‑148 (MQ=255)
                                      aactGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGaaa                                                                                                       >  1:1326370/1‑148 (MQ=255)
                                          gTTATTCATGGAGTATCAGCAAAGACTGTTATTTTATCTTTGGAGGTGCCTATCCAATGAAATACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCCCATTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGaaaaaag                                                                                                   >  2:1318316/1‑146 (MQ=255)
                                          gTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATg                                                                                                   >  1:2109611/1‑148 (MQ=255)
                                          gTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATg                                                                                                   >  1:1086901/1‑148 (MQ=255)
                                          gTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATg                                                                                                   >  1:1607534/1‑148 (MQ=255)
                                          gTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATg                                                                                                   >  1:180378/1‑148 (MQ=255)
                                           ttATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGc                                                                                                  <  1:1887784/148‑1 (MQ=255)
                                            tATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCa                                                                                                 <  2:642770/148‑1 (MQ=255)
                                            tATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCa                                                                                                 <  2:1686723/148‑1 (MQ=255)
                                               tCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACgg                                                                                              >  2:39557/1‑148 (MQ=255)
                                                  tGGAGTATCAGTAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGc                                                                                           <  2:2339417/148‑1 (MQ=255)
                                                  tGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGc                                                                                           >  1:2579831/1‑148 (MQ=255)
                                                  tGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGc                                                                                           >  1:2579783/1‑148 (MQ=255)
                                                    gAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTg                                                                                         <  2:536783/148‑1 (MQ=255)
                                                    gAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTg                                                                                         <  2:535993/148‑1 (MQ=255)
                                                    gAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTg                                                                                         <  1:502552/148‑1 (MQ=255)
                                                    gAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTg                                                                                         <  2:748060/148‑1 (MQ=255)
                                                     aGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGt                                                                                        <  1:2486085/148‑1 (MQ=255)
                                                     aGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGt                                                                                        <  2:2112326/148‑1 (MQ=255)
                                                       tATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCATATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGttt                                                                                      <  1:3313685/148‑1 (MQ=255)
                                                        aTCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTc                                                                                     >  1:1447759/1‑148 (MQ=255)
                                                         tCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTcc                                                                                    >  2:3216542/1‑148 (MQ=255)
                                                         tCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTcc                                                                                    <  2:3699887/148‑1 (MQ=255)
                                                          cAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTccc                                                                                   <  2:3572310/148‑1 (MQ=255)
                                                           aGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCt                                                                                  <  2:1473331/148‑1 (MQ=255)
                                                            gCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTc                                                                                 >  1:2173580/1‑148 (MQ=255)
                                                             cAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTcg                                                                                >  2:240817/1‑148 (MQ=255)
                                                             cAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTcg                                                                                >  1:3446805/1‑148 (MQ=255)
                                                             cAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTcg                                                                                <  2:3491189/148‑1 (MQ=255)
                                                                aGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCgcgc                                                                             >  2:2664048/1‑148 (MQ=255)
                                                                aGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCgcgc                                                                             >  2:1848189/1‑148 (MQ=255)
                                                                aGACGGTTATTTTATCTATGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTATCCCTCgcgc                                                                             >  2:961811/1‑148 (MQ=255)
                                                                 gACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCt                                                                            >  2:1280281/1‑148 (MQ=255)
                                                                       tATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAgg                                                                      <  1:863883/148‑1 (MQ=255)
                                                                       tATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAgg                                                                      <  1:334706/148‑1 (MQ=255)
                                                                       tATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAgg                                                                      >  1:1622576/1‑148 (MQ=255)
                                                                             aTCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTg                                                                >  2:230029/1‑148 (MQ=255)
                                                                              tCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGt                                                               >  1:699877/1‑148 (MQ=255)
                                                                               cTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTc                                                              <  1:3592777/148‑1 (MQ=255)
                                                                                 ttGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTg                                                            >  1:2612247/1‑148 (MQ=255)
                                                                                 ttGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTg                                                            <  1:1981549/148‑1 (MQ=255)
                                                                                   ggCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCg                                                          >  2:2803354/1‑148 (MQ=255)
                                                                                    gCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTAGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCgg                                                         <  2:2763773/148‑1 (MQ=255)
                                                                                            tATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACa                                                 >  1:3778766/1‑148 (MQ=255)
                                                                                             aTCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACaa                                                <  2:872563/148‑1 (MQ=255)
                                                                                               ccAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACg                                              >  1:129195/1‑148 (MQ=255)
                                                                                                 aaTGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGcc                                            <  2:587505/148‑1 (MQ=255)
                                                                                                  aTGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCt                                           <  1:3391750/148‑1 (MQ=255)
                                                                                                   tGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCtt                                          >  2:2551533/1‑148 (MQ=255)
                                                                                                   tGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCtt                                          >  1:1190813/1‑148 (MQ=255)
                                                                                                        gACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGa                                     <  2:91950/148‑1 (MQ=255)
                                                                                                        gACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGa                                     >  1:1144925/1‑148 (MQ=255)
                                                                                                        gACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTGTCTGa                                     >  1:3051620/1‑148 (MQ=255)
                                                                                                         aCGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGAt                                    <  2:769673/148‑1 (MQ=255)
                                                                                                         aCGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGAt                                    >  2:954985/1‑148 (MQ=255)
                                                                                                           ggTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTg                                  <  1:221797/148‑1 (MQ=255)
                                                                                                             tCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGt                                <  2:2729865/148‑1 (MQ=255)
                                                                                                              cAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTg                               >  1:2075977/1‑148 (MQ=255)
                                                                                                                gACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGaa                             >  1:3083380/1‑148 (MQ=255)
                                                                                                                gACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGaa                             >  1:2518893/1‑148 (MQ=255)
                                                                                                                 aCGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAg                            >  1:322412/1‑148 (MQ=255)
                                                                                                                 aCGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAg                            >  2:3383877/1‑148 (MQ=255)
                                                                                                                      tttCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAgcggcg                       >  1:797415/1‑148 (MQ=255)
                                                                                                                        tCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCggg                     >  1:57661/1‑148 (MQ=255)
                                                                                                                           cgacgCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGAtt                  >  1:3248018/1‑148 (MQ=255)
                                                                                                                           cgacgCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGAtt                  <  2:1137962/148‑1 (MQ=255)
                                                                                                                           cgacgCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTATCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGAtt                  >  1:2540585/1‑148 (MQ=255)
                                                                                                                             acgCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTa                <  2:1207841/148‑1 (MQ=255)
                                                                                                                                   aaaGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAgcg          >  1:3692218/1‑148 (MQ=255)
                                                                                                                                   aaaGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAgcg          >  2:2309752/1‑148 (MQ=255)
                                                                                                                                   aaaGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAgcg          >  1:952206/1‑148 (MQ=255)
                                                                                                                                     agagAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGcc        >  1:589686/1‑148 (MQ=255)
                                                                                                                                     agagAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGcc        >  2:103183/1‑148 (MQ=255)
                                                                                                                                     agagAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGGTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTCAGGACAACGCCTTTCTGAGTGGTGAAGCGGCGGGATTTATCAGCGcc        >  1:2452768/1‑148 (MQ=255)
                                                                                                                                         aaaaTTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCAGCt    >  1:3159219/1‑148 (MQ=255)
                                                                                                                                           aaTTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCAGCTCg  <  2:3521377/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                
TGCCGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCAGCTCG  >  CP009273/3863296‑3863582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCCGGTGCCGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCAGCTCGCTG  >  CP009273/3863290‑3863585
                                                                                                                                                    |                                                                                                                                                   
ATCCGGTGCCGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTA                                                                                                                                                    >  SRR19973069.251043/1‑150 (MQ=60)
 TCCGGTGCCGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAG                                                                                                                                                   <  SRR19973069.1074697/150‑1 (MQ=60)
 TCCGGTGCCGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAG                                                                                                                                                   <  SRR19973069.1562488/150‑1 (MQ=60)
 TCCGGTGCCGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAG                                                                                                                                                   <  SRR19973069.3197232/150‑1 (MQ=60)
    GGTGCCGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGC                                                                                                                                                >  SRR19973069.2471706/1‑150 (MQ=60)
    GGTGCCGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGC                                                                                                                                                >  SRR19973069.538122/1‑150 (MQ=60)
        CCGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTT                                                                                                                                            <  SRR19973069.1248730/150‑1 (MQ=60)
          GTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCA                                                                                                                                          <  SRR19973069.2860209/150‑1 (MQ=60)
          GTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCA                                                                                                                                          <  SRR19973069.3073720/150‑1 (MQ=60)
           TTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAG                                                                                                                                         <  SRR19973069.1513328/150‑1 (MQ=60)
               ACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCC                                                                                                                                     <  SRR19973069.2707245/150‑1 (MQ=60)
                    TGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTT                                                                                                                                <  SRR19973069.2956545/150‑1 (MQ=60)
                    TGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTT                                                                                                                                <  SRR19973069.2956601/150‑1 (MQ=60)
                     GCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTG                                                                                                                               <  SRR19973069.1284417/150‑1 (MQ=60)
                       ATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGT                                                                                                                             >  SRR19973069.2344564/1‑150 (MQ=60)
                       ATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGT                                                                                                                             <  SRR19973069.2521699/150‑1 (MQ=60)
                            TGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGAC                                                                                                                        <  SRR19973069.1272858/150‑1 (MQ=60)
                            TGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGAC                                                                                                                        <  SRR19973069.1273719/150‑1 (MQ=60)
                               TAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGT                                                                                                                     <  SRR19973069.3312166/150‑1 (MQ=60)
                                AATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTG                                                                                                                    >  SRR19973069.2758637/1‑150 (MQ=60)
                                AATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTG                                                                                                                    >  SRR19973069.1145011/1‑150 (MQ=60)
                                 ATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGA                                                                                                                   <  SRR19973069.3324976/150‑1 (MQ=60)
                                 ATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGA                                                                                                                   >  SRR19973069.229818/1‑150 (MQ=60)
                                  TTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAA                                                                                                                  <  SRR19973069.3322852/150‑1 (MQ=60)
                                    CGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAA                                                                                                                >  SRR19973069.2207328/1‑150 (MQ=60)
                                          CTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAA                                                                                                          >  SRR19973069.1335490/1‑150 (MQ=60)
                                          CTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAA                                                                                                          >  SRR19973069.1334153/1‑150 (MQ=60)
                                          CTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAA                                                                                                          >  SRR19973069.2505529/1‑150 (MQ=60)
                                           TAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAA                                                                                                         >  SRR19973069.1973544/1‑150 (MQ=60)
                                              CTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATG                                                                                                      >  SRR19973069.181882/1‑150 (MQ=60)
                                              CTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATG                                                                                                      >  SRR19973069.3527700/1‑150 (MQ=60)
                                              CTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATG                                                                                                      >  SRR19973069.1618360/1‑150 (MQ=60)
                                              CTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATG                                                                                                      >  SRR19973069.1618343/1‑150 (MQ=60)
                                              CTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATG                                                                                                      <  SRR19973069.1097006/150‑1 (MQ=60)
                                              CTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATG                                                                                                      >  SRR19973069.2124161/1‑150 (MQ=60)
                                              CTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATG                                                                                                      >  SRR19973069.1094490/1‑150 (MQ=60)
                                               TGTTATTCATGGAGTATCAGCAAAGAAGGTTATTTTATCTTTTGCGGTGCCTATCCAATGAAAGACTGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTAAGTTCAAGTTTGGTAAGACGGTGAAAAGCGAAAAATGA                                                                                                     >  SRR19973069.2678307/1‑150 (MQ=60)
                                                GTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCA                                                                                                    >  SRR19973069.1831872/1‑150 (MQ=60)
                                                 TTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCAC                                                                                                   <  SRR19973069.1900887/150‑1 (MQ=60)
                                                   ATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGG                                                                                                 >  SRR19973069.1145848/1‑150 (MQ=60)
                                                   ATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGG                                                                                                 >  SRR19973069.3684971/1‑150 (MQ=60)
                                                     TCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTG                                                                                               <  SRR19973069.3186793/150‑1 (MQ=60)
                                                      CATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGC                                                                                              >  SRR19973069.2596938/1‑150 (MQ=60)
                                                      CATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGC                                                                                              >  SRR19973069.2596972/1‑150 (MQ=60)
                                                      CATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGC                                                                                              >  SRR19973069.2596924/1‑150 (MQ=60)
                                                        TGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTG                                                                                            >  SRR19973069.852284/1‑150 (MQ=60)
                                                        TGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTG                                                                                            >  SRR19973069.852273/1‑150 (MQ=60)
                                                          GAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTT                                                                                          <  SRR19973069.506155/150‑1 (MQ=60)
                                                          GAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTT                                                                                          >  SRR19973069.3790696/1‑150 (MQ=60)
                                                           AGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTT                                                                                         <  SRR19973069.2502721/150‑1 (MQ=60)
                                                           AGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTT                                                                                         <  SRR19973069.2554086/150‑1 (MQ=60)
                                                           AGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTT                                                                                         >  SRR19973069.2114937/1‑150 (MQ=60)
                                                            GTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTC                                                                                        >  SRR19973069.1457865/1‑150 (MQ=60)
                                                            GTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTCTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTC                                                                                        >  SRR19973069.1458277/1‑150 (MQ=60)
                                                             TATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCATATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCC                                                                                       <  SRR19973069.3335404/150‑1 (MQ=60)
                                                                CAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTC                                                                                    >  SRR19973069.2188417/1‑150 (MQ=60)
                                                                CAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTC                                                                                    <  SRR19973069.212638/150‑1 (MQ=60)
                                                                CAGCAAAGACGGTTATTTTATCTTTGGCGGTGCATATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTC                                                                                    <  SRR19973069.2524953/150‑1 (MQ=60)
                                                                 AGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCG                                                                                   >  SRR19973069.3469162/1‑150 (MQ=60)
                                                                    AAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGC                                                                                >  SRR19973069.921/1‑150 (MQ=60)
                                                                       GACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGG                                                                             <  SRR19973069.617759/150‑1 (MQ=60)
                                                                          GGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAG                                                                          >  SRR19973069.2364091/1‑150 (MQ=60)
                                                                           GTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGG                                                                         >  SRR19973069.1216137/1‑150 (MQ=60)
                                                                           GTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGG                                                                         >  SRR19973069.1633591/1‑150 (MQ=60)
                                                                             TATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGAT                                                                       <  SRR19973069.3548701/150‑1 (MQ=60)
                                                                             TATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGAT                                                                       <  SRR19973069.337030/150‑1 (MQ=60)
                                                                             TATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGAT                                                                       <  SRR19973069.869917/150‑1 (MQ=60)
                                                                                 TTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTAGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTG                                                                   >  SRR19973069.1390891/1‑150 (MQ=60)
                                                                                  TATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCTGGATTTTGT                                                                  >  SRR19973069.705408/1‑150 (MQ=60)
                                                                                  TATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGT                                                                  >  SRR19973069.704794/1‑150 (MQ=60)
                                                                                     CTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTG                                                               <  SRR19973069.3616219/150‑1 (MQ=60)
                                                                                     CTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTG                                                               <  SRR19973069.304885/150‑1 (MQ=60)
                                                                                     CTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTG                                                               >  SRR19973069.2629712/1‑150 (MQ=60)
                                                                                       TTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCG                                                             <  SRR19973069.1995329/150‑1 (MQ=60)
                                                                                       TTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCG                                                             <  SRR19973069.50435/150‑1 (MQ=60)
                                                                                                CCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACA                                                    <  SRR19973069.1748091/150‑1 (MQ=60)
                                                                                                CCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACA                                                    >  SRR19973069.3341230/1‑150 (MQ=60)
                                                                                                CCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACA                                                    <  SRR19973069.2558632/150‑1 (MQ=60)
                                                                                                CCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACA                                                    >  SRR19973069.3803306/1‑150 (MQ=60)
                                                                                                   ATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACG                                                 >  SRR19973069.130100/1‑150 (MQ=60)
                                                                                                   ATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACG                                                 >  SRR19973069.130405/1‑150 (MQ=60)
                                                                                                     CCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCC                                               >  SRR19973069.3429861/1‑150 (MQ=60)
                                                                                                      CAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCT                                              <  SRR19973069.2344562/150‑1 (MQ=60)
                                                                                                       AATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTT                                             >  SRR19973069.1199024/1‑150 (MQ=60)
                                                                                                        ATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTT                                            <  SRR19973069.3413427/150‑1 (MQ=60)
                                                                                                        ATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTT                                            <  SRR19973069.3413899/150‑1 (MQ=60)
                                                                                                            AAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGA                                        >  SRR19973069.1152914/1‑150 (MQ=60)
                                                                                                            AAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGA                                        >  SRR19973069.3072078/1‑150 (MQ=60)
                                                                                                            AAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTGTCTGA                                        >  SRR19973069.3071815/1‑150 (MQ=60)
                                                                                                            AAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGAGTTTGTCTGCGGTAAGGACCACGCCTTTCTGA                                        >  SRR19973069.324664/1‑150 (MQ=60)
                                                                                                              GACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATT                                      <  SRR19973069.2799507/150‑1 (MQ=60)
                                                                                                                 GGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGT                                   <  SRR19973069.223511/150‑1 (MQ=60)
                                                                                                                  GTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTG                                  >  SRR19973069.2090352/1‑150 (MQ=60)
                                                                                                                    CAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAA                                >  SRR19973069.2535645/1‑150 (MQ=60)
                                                                                                                    CAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAA                                >  SRR19973069.3103713/1‑150 (MQ=60)
                                                                                                                     AGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAG                               >  SRR19973069.324549/1‑150 (MQ=60)
                                                                                                                       ACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCG                             <  SRR19973069.3153664/150‑1 (MQ=60)
                                                                                                                          CGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCG                          >  SRR19973069.803069/1‑150 (MQ=60)
                                                                                                                            TTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGG                        >  SRR19973069.58361/1‑150 (MQ=60)
                                                                                                                            TTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGG                        >  SRR19973069.58363/1‑150 (MQ=60)
                                                                                                                            TTTAACGACGCTGAAAGAGAAAATTAGCTCCTTTCAGATACAGTATTGTAAGACGTTGAAAAGCTAAAAAAGCACTGTTATGTTTCCCTCGCGCTGGCAGGATTTTGTCTGAGGTAAGGAAAACGCCTATCTGATTGGTGAAGAGGCGGG                        >  SRR19973069.57838/1‑150 (MQ=60)
                                                                                                                               CACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATT                     >  SRR19973069.3269283/1‑150 (MQ=60)
                                                                                                                               CACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTATCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATT                     >  SRR19973069.2557397/1‑150 (MQ=60)
                                                                                                                                    CGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCA                >  SRR19973069.3283337/1‑150 (MQ=60)
                                                                                                                                       TGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCG             >  SRR19973069.3716361/1‑150 (MQ=60)
                                                                                                                                       TGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCG             >  SRR19973069.958604/1‑150 (MQ=60)
                                                                                                                                        GAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGC            <  SRR19973069.2522511/150‑1 (MQ=60)
                                                                                                                                         AAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCC           >  SRR19973069.593706/1‑150 (MQ=60)
                                                                                                                                         AAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCC           >  SRR19973069.2612800/1‑150 (MQ=60)
                                                                                                                                         AAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGGTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTCAGGACAACGCCTTTCTGAGTGGTGAAGCGGCGGGATTTATCAGCGCC           >  SRR19973069.2469264/1‑150 (MQ=60)
                                                                                                                                          AAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCA          >  SRR19973069.1187742/1‑150 (MQ=60)
                                                                                                                                          AAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCA          >  SRR19973069.2079392/1‑150 (MQ=60)
                                                                                                                                          AAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCA          >  SRR19973069.2082127/1‑150 (MQ=60)
                                                                                                                                             AGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCAGCT       >  SRR19973069.3179838/1‑150 (MQ=60)
                                                                                                                                             AGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCAGCT       >  SRR19973069.974334/1‑150 (MQ=60)
                                                                                                                                                AAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCAGCTCGC    >  SRR19973069.2671769/1‑150 (MQ=60)
                                                                                                                                                AAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCAGCTCGC    >  SRR19973069.2671409/1‑150 (MQ=60)
                                                                                                                                                AAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCAGCTCGC    >  SRR19973069.2500284/1‑150 (MQ=60)
                                                                                                                                                AAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCAGCTCGC    >  SRR19973069.2496253/1‑150 (MQ=60)
                                                                                                                                                  ATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCAGCTCGCTG  >  SRR19973069.1493924/1‑150 (MQ=60)
                                                                                                                                                  ATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCAGCTCGCTG  >  SRR19973069.1494088/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
ATCCGGTGCCGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCAGCTCGCTG  >  CP009273/3863290‑3863585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑