Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,193,578 G→T 100% D111Y (GAT→TAT)  zraR → fused DNA‑binding response regulator in two‑component regulatory system with ZraS: response regulator/sigma54 interaction protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,193,5780GT98.5% 647.8 / 0.5 201D111Y (GAT→TAT) zraRfused DNA‑binding response regulator in two‑component regulatory system with ZraS: response regulator/sigma54 interaction protein
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (106/92);  total (107/94)
Fisher's exact test for biased strand distribution p-value = 6.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 202 total aligned reads displayed.

GACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCGATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCGTCG  >  CP009273/4193434‑4193722
                                                                                                                                                |                                                                                                                                                
 aCGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTGTCTCATCAAGCCGCTGGATTTCTATaa                                                                                                                                              >  1:2155848/1‑148 (MQ=255)
  cGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAAc                                                                                                                                             <  1:3363089/148‑1 (MQ=255)
        cGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAg                                                                                                                                       >  2:97454/1‑148 (MQ=255)
         gCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAgg                                                                                                                                      <  1:5228/148‑1 (MQ=255)
           cACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCg                                                                                                                                    >  2:3389362/1‑148 (MQ=255)
               cTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGc                                                                                                                                <  1:1139930/148‑1 (MQ=255)
               cTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGc                                                                                                                                <  1:9293/148‑1 (MQ=255)
               cTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGc                                                                                                                                <  1:3018686/148‑1 (MQ=255)
                 gAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTg                                                                                                                              >  2:3071429/1‑148 (MQ=255)
                     gagaTCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGaaa                                                                                                                          <  1:1290641/148‑1 (MQ=255)
                     gagaTCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGaaa                                                                                                                          <  1:296424/148‑1 (MQ=255)
                       gaTCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGaaaaa                                                                                                                        <  2:2496925/148‑1 (MQ=255)
                       gaTCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGaaaaa                                                                                                                        <  1:1920819/148‑1 (MQ=255)
                        aTCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAg                                                                                                                       >  1:435165/1‑148 (MQ=255)
                           aaaGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAgcgc                                                                                                                    <  2:451199/148‑1 (MQ=255)
                            aaGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCt                                                                                                                   >  2:1188469/1‑148 (MQ=255)
                             aGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTc                                                                                                                  >  1:669937/1‑148 (MQ=255)
                              gCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTcg                                                                                                                 <  2:2044610/148‑1 (MQ=255)
                              gCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTcg                                                                                                                 >  1:2875196/1‑148 (MQ=255)
                                 ttAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCgcgc                                                                                                              <  2:1163243/148‑1 (MQ=255)
                                 ttAAACCCGGCAATTCCGGAGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATACCCTACAGGCGACGCTGGAAAAAGCGCTCgcgc                                                                                                              >  2:2094688/1‑148 (MQ=255)
                                    aaCCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATa                                                                                                           <  2:1904104/148‑1 (MQ=255)
                                    aaCCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATa                                                                                                           <  2:2203499/148‑1 (MQ=255)
                                    aaCCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATa                                                                                                           <  2:2343862/148‑1 (MQ=255)
                                    aaCCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATa                                                                                                           >  1:2737226/1‑148 (MQ=255)
                                     aCCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATAc                                                                                                          >  2:656890/1‑148 (MQ=255)
                                     aCCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATAc                                                                                                          >  2:2728010/1‑148 (MQ=255)
                                      cccGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACg                                                                                                         >  1:1655699/1‑148 (MQ=255)
                                       ccGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGc                                                                                                        <  2:2738585/148‑1 (MQ=255)
                                       ccGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGc                                                                                                        <  1:605977/148‑1 (MQ=255)
                                       ccGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGc                                                                                                        <  2:2740025/148‑1 (MQ=255)
                                       ccGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCGGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGc                                                                                                        <  2:2009185/148‑1 (MQ=255)
                                          gCAATTCCGGTGCTGATTATGACTGCGTACCCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGcaca                                                                                                     <  2:2861557/148‑1 (MQ=255)
                                           cAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAg                                                                                                    <  2:1825296/148‑1 (MQ=255)
                                            aaTTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGt                                                                                                   <  2:3482536/148‑1 (MQ=255)
                                            aaTTCCGGTGCTGATTATGACGGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGt                                                                                                   <  2:3091452/148‑1 (MQ=255)
                                             aTTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTa                                                                                                  <  1:1900758/148‑1 (MQ=255)
                                               tCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTAtt                                                                                                >  1:184123/1‑148 (MQ=255)
                                                ccGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTg                                                                                               <  2:1315631/148‑1 (MQ=255)
                                                       tGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGa                                                                                        >  1:1493510/1‑148 (MQ=255)
                                                       tGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGa                                                                                        >  1:976650/1‑148 (MQ=255)
                                                        gATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGaa                                                                                       >  2:706767/1‑148 (MQ=255)
                                                           tATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAaca                                                                                    >  2:1582000/1‑148 (MQ=255)
                                                             tGACTGCGTACTCCAGTGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACAcc                                                                                  <  2:1973090/148‑1 (MQ=255)
                                                             ggACTGCGTACTCCAGTGTCGAGACGGCGGTAGAGGCACTGAACACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACAcc                                                                                  <  2:2523224/147‑1 (MQ=255)
                                                                     tACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGa                                                                          <  2:1463033/148‑1 (MQ=255)
                                                                      aCTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGAc                                                                         >  2:1774642/1‑148 (MQ=255)
                                                                      aCTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGAc                                                                         >  2:1172059/1‑148 (MQ=255)
                                                                        tCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTg                                                                       >  1:3165389/1‑148 (MQ=255)
                                                                         ccAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTgc                                                                      <  1:2904358/148‑1 (MQ=255)
                                                                          cAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTgcc                                                                     >  1:2619232/1‑148 (MQ=255)
                                                                                 gagaCGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGt                                                              >  2:2936829/1‑148 (MQ=255)
                                                                                  agaCGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGGGACTGCCAGCCAGtt                                                             >  2:64404/1‑148 (MQ=255)
                                                                                       gcggTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTa                                                        <  1:1392638/148‑1 (MQ=255)
                                                                                          gTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATgg                                                     <  1:712896/148‑1 (MQ=255)
                                                                                          gTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATgg                                                     <  1:1828447/148‑1 (MQ=255)
                                                                                            agagGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTc                                                   >  1:3212562/1‑148 (MQ=255)
                                                                                             gagGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCg                                                  >  2:2647839/1‑148 (MQ=255)
                                                                                              agGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCgg                                                 >  2:2312506/1‑148 (MQ=255)
                                                                                              agGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCgg                                                 >  2:891805/1‑148 (MQ=255)
                                                                                               tgCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTATATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGt                                                <  1:3464316/147‑1 (MQ=255)
                                                                                               ggCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGt                                                <  1:1548286/148‑1 (MQ=255)
                                                                                                  aCTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTaaa                                             <  1:2256894/148‑1 (MQ=255)
                                                                                                     gAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGc                                          >  1:1772454/1‑148 (MQ=255)
                                                                                                      aaaaCTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGcc                                         >  2:3011705/1‑148 (MQ=255)
                                                                                                      aaaaCTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGcc                                         <  1:249768/148‑1 (MQ=255)
                                                                                                       aaaCTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGccc                                        >  1:1697275/1‑148 (MQ=255)
                                                                                                       aaaCTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGccc                                        >  2:2715153/1‑148 (MQ=255)
                                                                                                        aaCTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCg                                       >  2:41515/1‑148 (MQ=255)
                                                                                                         aCTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCgg                                      >  2:1533970/1‑148 (MQ=255)
                                                                                                         aCTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCgg                                      >  1:478607/1‑148 (MQ=255)
                                                                                                         aCTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCgg                                      >  2:913908/1‑148 (MQ=255)
                                                                                                         aCTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCgg                                      >  1:1004534/1‑148 (MQ=255)
                                                                                                         aCTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCgg                                      >  2:1748621/1‑148 (MQ=255)
                                                                                                           tGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCg                                    >  1:703875/1‑148 (MQ=255)
                                                                                                            ggggCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGa                                   >  2:653346/1‑148 (MQ=255)
                                                                                                             gggCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGAt                                  <  2:2394807/148‑1 (MQ=255)
                                                                                                             gggCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGAt                                  >  1:1119612/1‑148 (MQ=255)
                                                                                                             gggCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGAt                                  <  2:1228285/148‑1 (MQ=255)
                                                                                                                cgcTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCa                               <  1:3193140/148‑1 (MQ=255)
                                                                                                                  cTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAac                             <  2:3123540/148‑1 (MQ=255)
                                                                                                                    ggATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGcaccac                           >  1:2710849/1‑148 (MQ=255)
                                                                                                                    ggATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAacac                           >  1:2710779/1‑148 (MQ=255)
                                                                                                                     gATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACAcc                          <  1:1012984/148‑1 (MQ=255)
                                                                                                                       ttATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGACAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTg                        <  2:236641/148‑1 (MQ=255)
                                                                                                                           ctcATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCa                    >  2:1056628/1‑148 (MQ=255)
                                                                                                                            tcatcaAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAg                   >  2:1031716/1‑148 (MQ=255)
                                                                                                                               tcaAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACATGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGa                >  2:2485646/1‑148 (MQ=255)
                                                                                                                                caAGCCGCTGGATTTCGATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGaa               <  2:1276999/148‑1 (MQ=255)
                                                                                                                                 aaGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGaaa              <  2:2619232/148‑1 (MQ=255)
                                                                                                                                 aaGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGaaa              <  1:2347134/148‑1 (MQ=255)
                                                                                                                                  aGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAAt             <  2:2875196/148‑1 (MQ=255)
                                                                                                                                  aGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAAt             >  1:1019353/1‑148 (MQ=255)
                                                                                                                                   gCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATc            >  2:2510038/1‑148 (MQ=255)
                                                                                                                                    ccGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCg           <  2:3102616/148‑1 (MQ=255)
                                                                                                                                           aTTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCtcgt    <  1:653346/148‑1 (MQ=255)
                                                                                                                                           aTTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCtcgt    <  1:3108589/148‑1 (MQ=255)
                                                                                                                                            tttCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATAGACCtcgtc   >  2:949452/1‑148 (MQ=255)
                                                                                                                                             ttCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCtcgtcg  >  1:3128597/1‑148 (MQ=255)
                                                                                                                                             ttCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCtcgtcg  >  1:832888/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCGATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCGTCG  >  CP009273/4193434‑4193722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGATGGACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCGATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCGTCGCGCCA  >  CP009273/4193429‑4193727
                                                                                                                                                     |                                                                                                                                                     
AGATGGACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCT                                                                                                                                                       <  SRR19973065.3286427/150‑1 (MQ=60)
AGATGGACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCT                                                                                                                                                       <  SRR19973065.3286428/150‑1 (MQ=60)
 GATGGACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTA                                                                                                                                                      <  SRR19973065.1701000/150‑1 (MQ=60)
    GGACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTGTCTCATCAAGCCGCTGGATTTCTATAA                                                                                                                                                   >  SRR19973065.2169712/1‑150 (MQ=60)
     GACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAAC                                                                                                                                                  <  SRR19973065.1020002/150‑1 (MQ=60)
       CGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCT                                                                                                                                                <  SRR19973065.3383650/150‑1 (MQ=60)
         GCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTAC                                                                                                                                              <  SRR19973065.2725320/150‑1 (MQ=60)
              GCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCG                                                                                                                                         <  SRR19973065.5306/150‑1 (MQ=60)
                  CGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGC                                                                                                                                     >  SRR19973065.568066/1‑150 (MQ=60)
                    CTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTG                                                                                                                                   <  SRR19973065.9439/150‑1 (MQ=60)
                    CTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTG                                                                                                                                   <  SRR19973065.1147306/150‑1 (MQ=60)
                    CTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTG                                                                                                                                   <  SRR19973065.3036383/150‑1 (MQ=60)
                    CTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTG                                                                                                                                   <  SRR19973065.3037181/150‑1 (MQ=60)
                         AGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAA                                                                                                                              >  SRR19973065.987951/1‑150 (MQ=60)
                          GAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAA                                                                                                                             <  SRR19973065.1299149/150‑1 (MQ=60)
                          GAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAA                                                                                                                             <  SRR19973065.298331/150‑1 (MQ=60)
                           AGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAG                                                                                                                            >  SRR19973065.438191/1‑150 (MQ=60)
                            GATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGC                                                                                                                           <  SRR19973065.1933416/150‑1 (MQ=60)
                             ATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCG                                                                                                                          <  SRR19973065.847988/150‑1 (MQ=60)
                             ATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCG                                                                                                                          <  SRR19973065.2454062/150‑1 (MQ=60)
                               CAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCT                                                                                                                        <  SRR19973065.316167/150‑1 (MQ=60)
                                AAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTC                                                                                                                       >  SRR19973065.674402/1‑150 (MQ=60)
                                 AAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCG                                                                                                                      >  SRR19973065.2892810/1‑150 (MQ=60)
                                 AAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTATATAACCTACAGGCGACGCTGGAAAAAGCGCTCG                                                                                                                      <  SRR19973065.346214/150‑1 (MQ=60)
                                   GCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCG                                                                                                                    >  SRR19973065.1729266/1‑150 (MQ=60)
                                     GTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGTGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCA                                                                                                                  >  SRR19973065.3142561/1‑150 (MQ=60)
                                       TAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATA                                                                                                                >  SRR19973065.2754253/1‑150 (MQ=60)
                                         AACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACG                                                                                                              >  SRR19973065.1666796/1‑150 (MQ=60)
                                            CCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCAC                                                                                                           <  SRR19973065.609819/150‑1 (MQ=60)
                                            CCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCAC                                                                                                           <  SRR19973065.3214350/150‑1 (MQ=60)
                                                 AATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTAT                                                                                                      >  SRR19973065.736819/1‑150 (MQ=60)
                                                  ATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATT                                                                                                     <  SRR19973065.1912983/150‑1 (MQ=60)
                                                  ATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATT                                                                                                     <  SRR19973065.1913244/150‑1 (MQ=60)
                                                  ATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATT                                                                                                     >  SRR19973065.185576/1‑150 (MQ=60)
                                                   TTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTG                                                                                                    <  SRR19973065.1617815/150‑1 (MQ=60)
                                                        GTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCT                                                                                               >  SRR19973065.261978/1‑150 (MQ=60)
                                                          GCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGA                                                                                             >  SRR19973065.1503101/1‑150 (MQ=60)
                                                          GCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGA                                                                                             >  SRR19973065.983136/1‑150 (MQ=60)
                                                           CTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAA                                                                                            >  SRR19973065.1054365/1‑150 (MQ=60)
                                                                       GCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTG                                                                                <  SRR19973065.3063875/150‑1 (MQ=60)
                                                                           ACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTG                                                                            >  SRR19973065.3185208/1‑150 (MQ=60)
                                                                           ACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTG                                                                            >  SRR19973065.3184563/1‑150 (MQ=60)
                                                                             TCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCC                                                                          >  SRR19973065.2635449/1‑150 (MQ=60)
                                                                              CCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCA                                                                         <  SRR19973065.2922065/150‑1 (MQ=60)
                                                                               CAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAG                                                                        >  SRR19973065.1977437/1‑150 (MQ=60)
                                                                                            GCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATG                                                           <  SRR19973065.1401780/150‑1 (MQ=60)
                                                                                            GCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATG                                                           <  SRR19973065.1402037/150‑1 (MQ=60)
                                                                                              GGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGT                                                         <  SRR19973065.2740593/150‑1 (MQ=60)
                                                                                               GTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTC                                                        <  SRR19973065.1840491/150‑1 (MQ=60)
                                                                                               GTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTC                                                        >  SRR19973065.3231887/1‑150 (MQ=60)
                                                                                               GTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTC                                                        <  SRR19973065.717666/150‑1 (MQ=60)
                                                                                               GTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTC                                                        <  SRR19973065.716901/150‑1 (MQ=60)
                                                                                                    TGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTATATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAA                                                   <  SRR19973065.3485415/150‑1 (MQ=60)
                                                                                                    GGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAA                                                   <  SRR19973065.1558530/150‑1 (MQ=60)
                                                                                                    GGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAA                                                   <  SRR19973065.1558533/150‑1 (MQ=60)
                                                                                                    GGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTATATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAA                                                   >  SRR19973065.27765/1‑150 (MQ=60)
                                                                                                      CACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCAAACGCACAGTATTGATGCTGAAACACCAGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAA                                                 >  SRR19973065.3258872/1‑150 (MQ=60)
                                                                                                       ACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAG                                                <  SRR19973065.2271178/150‑1 (MQ=60)
                                                                                                        CTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGC                                               >  SRR19973065.1784055/1‑150 (MQ=60)
                                                                                                          GAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACCGGATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCC                                             >  SRR19973065.1801616/1‑150 (MQ=60)
                                                                                                          GAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCC                                             >  SRR19973065.1708600/1‑150 (MQ=60)
                                                                                                           AAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCG                                            <  SRR19973065.251522/150‑1 (MQ=60)
                                                                                                            AAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGG                                           >  SRR19973065.481867/1‑150 (MQ=60)
                                                                                                            AAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGG                                           >  SRR19973065.481869/1‑150 (MQ=60)
                                                                                                            AAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGG                                           >  SRR19973065.1011203/1‑150 (MQ=60)
                                                                                                              ACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCG                                         >  SRR19973065.2269303/1‑150 (MQ=60)
                                                                                                              ACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCG                                         >  SRR19973065.708522/1‑150 (MQ=60)
                                                                                                              ACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCG                                         >  SRR19973065.2272562/1‑150 (MQ=60)
                                                                                                              ACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCG                                         >  SRR19973065.708556/1‑150 (MQ=60)
                                                                                                                TGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGAT                                       >  SRR19973065.1126915/1‑150 (MQ=60)
                                                                                                                TGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGAT                                       >  SRR19973065.1127228/1‑150 (MQ=60)
                                                                                                                     CGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAAC                                  <  SRR19973065.3212396/150‑1 (MQ=60)
                                                                                                                     CGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAAC                                  >  SRR19973065.2968393/1‑150 (MQ=60)
                                                                                                                       CTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCACCAC                                >  SRR19973065.2727704/1‑150 (MQ=60)
                                                                                                                       CTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACAC                                >  SRR19973065.2727690/1‑150 (MQ=60)
                                                                                                                       CTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACAC                                >  SRR19973065.2727634/1‑150 (MQ=60)
                                                                                                                         GGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCT                              <  SRR19973065.98354/150‑1 (MQ=60)
                                                                                                                          GATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTG                             <  SRR19973065.1019698/150‑1 (MQ=60)
                                                                                                                          GATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTG                             <  SRR19973065.1019645/150‑1 (MQ=60)
                                                                                                                          GATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTG                             >  SRR19973065.290345/1‑150 (MQ=60)
                                                                                                                            TTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCT                           <  SRR19973065.1918298/150‑1 (MQ=60)
                                                                                                                                 TCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTG                      <  SRR19973065.380778/150‑1 (MQ=60)
                                                                                                                                   ATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAA                    <  SRR19973065.1672085/150‑1 (MQ=60)
                                                                                                                                     CAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAAT                  >  SRR19973065.1026113/1‑150 (MQ=60)
                                                                                                                                      AAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATC                 <  SRR19973065.2361766/150‑1 (MQ=60)
                                                                                                                                             TGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCG          >  SRR19973065.52708/1‑150 (MQ=60)
                                                                                                                                             TGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCG          >  SRR19973065.1845355/1‑150 (MQ=60)
                                                                                                                                             TGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCG          >  SRR19973065.1178821/1‑150 (MQ=60)
                                                                                                                                               GATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCGTC        >  SRR19973065.1301102/1‑150 (MQ=60)
                                                                                                                                                ATTTCTATACCCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCGTCG       <  SRR19973065.657650/150‑1 (MQ=60)
                                                                                                                                                ATTTCTATAACCTACCGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCGTCG       <  SRR19973065.657220/150‑1 (MQ=60)
                                                                                                                                                ATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCGTCG       <  SRR19973065.657753/150‑1 (MQ=60)
                                                                                                                                                ATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCGTCG       >  SRR19973065.3147641/1‑150 (MQ=60)
                                                                                                                                                ATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCGTCG       <  SRR19973065.3127546/150‑1 (MQ=60)
                                                                                                                                                ATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCGTCG       >  SRR19973065.838324/1‑150 (MQ=60)
                                                                                                                                                    CTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCGTCGCGCC   >  SRR19973065.2251281/1‑150 (MQ=60)
                                                                                                                                                    CTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCGTCGCGCC   >  SRR19973065.2251032/1‑150 (MQ=60)
                                                                                                                                                     TATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCGTCGCGCCA  >  SRR19973065.2947296/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
AGATGGACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCGATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCGTCGCGCCA  >  CP009273/4193429‑4193727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑