Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,193,578 G→T 100% D111Y (GAT→TAT)  zraR → fused DNA‑binding response regulator in two‑component regulatory system with ZraS: response regulator/sigma54 interaction protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,193,5780GT98.7% 715.5 / 0.5 222D111Y (GAT→TAT) zraRfused DNA‑binding response regulator in two‑component regulatory system with ZraS: response regulator/sigma54 interaction protein
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (111/108);  total (113/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 224 total aligned reads displayed.

GGACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCGATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCGTCG  >  CP009273/4193433‑4193722
                                                                                                                                                 |                                                                                                                                                
ggACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTAt                                                                                                                                                >  1:2651528/1‑148 (MQ=255)
 gACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCtata                                                                                                                                               <  2:18422/148‑1 (MQ=255)
   cGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAAc                                                                                                                                             <  2:3463256/148‑1 (MQ=255)
   cGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAAc                                                                                                                                             <  1:1260773/148‑1 (MQ=255)
     gCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCt                                                                                                                                           <  1:3142927/148‑1 (MQ=255)
              cGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGAc                                                                                                                                  >  2:1979713/1‑148 (MQ=255)
               gCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACg                                                                                                                                 <  2:616836/148‑1 (MQ=255)
               gCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACg                                                                                                                                 <  2:698311/148‑1 (MQ=255)
                  gAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTg                                                                                                                              >  2:1135836/1‑148 (MQ=255)
                  gAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTg                                                                                                                              <  2:375446/148‑1 (MQ=255)
                  gAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTg                                                                                                                              >  2:3143187/1‑148 (MQ=255)
                   aaaGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTgg                                                                                                                             >  1:3313817/1‑148 (MQ=255)
                       agaTCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGaaaa                                                                                                                         <  2:158134/148‑1 (MQ=255)
                       agaTCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGaaaa                                                                                                                         >  1:22293/1‑148 (MQ=255)
                       agaTCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGaaaa                                                                                                                         >  1:22021/1‑148 (MQ=255)
                       agaTCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGaaaa                                                                                                                         <  2:2212831/148‑1 (MQ=255)
                         aTCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAg                                                                                                                       >  1:3360479/1‑148 (MQ=255)
                           cacaGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAgcg                                                                                                                     <  2:1167000/145‑1 (MQ=255)
                           cAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAgcg                                                                                                                     <  1:2350330/148‑1 (MQ=255)
                           cAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAgcg                                                                                                                     >  2:1752788/1‑148 (MQ=255)
                             aaGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCt                                                                                                                   <  2:2270560/148‑1 (MQ=255)
                                cGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTcgc                                                                                                                >  2:368004/1‑148 (MQ=255)
                                cGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTcgc                                                                                                                >  1:3565450/1‑148 (MQ=255)
                                  ttAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCgcgc                                                                                                              >  1:107158/1‑148 (MQ=255)
                                       cccGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACg                                                                                                         >  1:2442311/1‑148 (MQ=255)
                                           gCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGcaca                                                                                                     <  2:1622951/148‑1 (MQ=255)
                                            cAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAg                                                                                                    >  1:736220/1‑148 (MQ=255)
                                              agtCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTa                                                                                                  <  2:1525956/146‑1 (MQ=255)
                                              aTTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTa                                                                                                  >  1:1265561/1‑148 (MQ=255)
                                               ttCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTAt                                                                                                 >  2:581516/1‑148 (MQ=255)
                                               ttCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTAt                                                                                                 >  2:3711116/1‑148 (MQ=255)
                                                 ccGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTg                                                                                               <  2:1461481/148‑1 (MQ=255)
                                                     tGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGc                                                                                           <  1:36298/148‑1 (MQ=255)
                                                     tGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGc                                                                                           <  1:1243178/148‑1 (MQ=255)
                                                      gCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCt                                                                                          >  2:640392/1‑148 (MQ=255)
                                                      gCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCt                                                                                          >  2:3450375/1‑148 (MQ=255)
                                                         gATTATGACTGCGTACTCCAGCGTCTAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGAGGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGaa                                                                                       <  1:2020026/148‑1 (MQ=255)
                                                         gATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCGATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGaa                                                                                       >  1:734118/1‑148 (MQ=255)
                                                           ttATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAac                                                                                     <  2:3815756/148‑1 (MQ=255)
                                                             aTGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAacac                                                                                   <  1:1407186/148‑1 (MQ=255)
                                                              tGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACAcc                                                                                  <  1:3145900/148‑1 (MQ=255)
                                                                  tGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCg                                                                              <  2:2205281/148‑1 (MQ=255)
                                                                      tACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGa                                                                          >  1:3059971/1‑148 (MQ=255)
                                                                      tACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGa                                                                          <  1:2861942/148‑1 (MQ=255)
                                                                        cTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACt                                                                        >  2:2812929/1‑148 (MQ=255)
                                                                          ccAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGTAACACCTGCGGTGACTgc                                                                      >  1:1324107/1‑148 (MQ=255)
                                                                          ccAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTgc                                                                      >  1:1078476/1‑148 (MQ=255)
                                                                           cAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTgcc                                                                     >  1:356818/1‑148 (MQ=255)
                                                                             gCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTgccag                                                                   >  2:1248185/1‑148 (MQ=255)
                                                                              cGTCGAGACGGCGGTAGAGGCACTGAACACTGGGGCGCTGGATTATCTCATCAAGCCGCGGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTgccagc                                                                  <  2:3536668/148‑1 (MQ=255)
                                                                              cGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTgccagc                                                                  <  1:1154984/148‑1 (MQ=255)
                                                                                 cGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGccagccag                                                               <  1:3789852/148‑1 (MQ=255)
                                                                                 cGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGccagccag                                                               >  2:2995285/1‑148 (MQ=255)
                                                                                  gagaCGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGt                                                              <  1:2457680/148‑1 (MQ=255)
                                                                                  gagaCGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGt                                                              <  2:2958274/148‑1 (MQ=255)
                                                                                   agaCGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGtt                                                             >  1:2067633/1‑148 (MQ=255)
                                                                                   agaCGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGtt                                                             >  1:3183031/1‑148 (MQ=255)
                                                                                    gaCGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTc                                                            >  1:2047268/1‑148 (MQ=255)
                                                                                      cggcggTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCgg                                                          >  2:526135/1‑148 (MQ=255)
                                                                                          ggTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATg                                                      <  2:1265539/148‑1 (MQ=255)
                                                                                             agagGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTc                                                   >  1:3076277/1‑148 (MQ=255)
                                                                                                ggCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGt                                                >  2:2609098/1‑148 (MQ=255)
                                                                                                ggCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGt                                                >  2:2608986/1‑148 (MQ=255)
                                                                                                  cACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTaa                                              >  2:1879539/1‑148 (MQ=255)
                                                                                                   aCTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTaaa                                             <  1:1131877/148‑1 (MQ=255)
                                                                                                    cTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTaaaa                                            >  2:1702402/1‑148 (MQ=255)
                                                                                                     tGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAg                                           <  2:3360479/148‑1 (MQ=255)
                                                                                                     tGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAg                                           <  2:252176/148‑1 (MQ=255)
                                                                                                          aCTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAg                                           <  1:2030004/143‑1 (MQ=255)
                                                                                                          aCTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAg                                           >  2:2030004/1‑143 (MQ=255)
                                                                                                             ggggCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGa                                   <  1:1177148/148‑1 (MQ=255)
                                                                                                             ggggCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGa                                   <  1:1552572/148‑1 (MQ=255)
                                                                                                             ggggCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGa                                   <  1:2325219/148‑1 (MQ=255)
                                                                                                             ggggCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGa                                   <  2:2056720/148‑1 (MQ=255)
                                                                                                               ggCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATg                                 <  2:2047891/148‑1 (MQ=255)
                                                                                                                   cTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAac                             >  1:2918419/1‑148 (MQ=255)
                                                                                                                   cTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAac                             <  2:3040294/148‑1 (MQ=255)
                                                                                                                     ggATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAacac                           <  2:1878180/148‑1 (MQ=255)
                                                                                                                     ggATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAacac                           >  1:2994636/1‑148 (MQ=255)
                                                                                                                     ggATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAacac                           >  1:2996689/1‑148 (MQ=255)
                                                                                                                     ggATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAacac                           >  1:1603719/1‑148 (MQ=255)
                                                                                                                      gATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACAcc                          >  1:2888331/1‑148 (MQ=255)
                                                                                                                        ttATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTg                        >  1:2582705/1‑148 (MQ=255)
                                                                                                                            ctcATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCa                    >  2:1495755/1‑148 (MQ=255)
                                                                                                                             tcatcaAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCACCACCTGCTCAg                   <  2:451175/148‑1 (MQ=255)
                                                                                                                             tcatcaAGCCGCTGGATTTCTACAACCTACCGGCGCCGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAg                   <  2:3507037/148‑1 (MQ=255)
                                                                                                                              catcaAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGt                  >  2:3026073/1‑148 (MQ=255)
                                                                                                                               ataaaGCCGCTGGATTTATATAAACTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATAGATTCTGAAACACCAGCGGTGACTGCAAGACAGATCGGAATGGTCGGTAAAATACAGGAGATGCCACACCTGCTCAATg                 >  2:542255/4‑148 (MQ=255)
                                                                                                                                tcaAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGa                >  1:3235121/1‑148 (MQ=255)
                                                                                                                                   aGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAAt             <  2:232371/148‑1 (MQ=255)
                                                                                                                                    gCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATc            >  2:1026089/1‑148 (MQ=255)
                                                                                                                                       tatGGATTTCTATAACCTACTGGCTACGCTGTAAAAAGCGCTCGCGCATAAGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGACCGGCGATGCAACACCTGCTCAGTGAAATCGcc         <  1:3693384/146‑1 (MQ=255)
                                                                                                                                       gCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGcc         <  2:1324107/148‑1 (MQ=255)
                                                                                                                                        cTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGccc        <  1:1926053/148‑1 (MQ=255)
                                                                                                                                         tGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCt       <  2:107311/148‑1 (MQ=255)
                                                                                                                                          ggATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCtc      >  1:3751092/1‑148 (MQ=255)
                                                                                                                                            aTTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCtcgt    >  1:902840/1‑148 (MQ=255)
                                                                                                                                            aTTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCtcgt    >  1:490541/1‑148 (MQ=255)
                                                                                                                                            aTTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCtcgt    <  2:846734/148‑1 (MQ=255)
                                                                                                                                             tttCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCtcgtc   >  1:3780265/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCGATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCGTCG  >  CP009273/4193433‑4193722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGGACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCGATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCGTCGCGCCA  >  CP009273/4193431‑4193727
                                                                                                                                                   |                                                                                                                                                     
ATGGACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTAT                                                                                                                                                     >  SRR19973069.2669398/1‑150 (MQ=60)
ATGGACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTAT                                                                                                                                                     >  SRR19973069.2669291/1‑150 (MQ=60)
 TGGACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATA                                                                                                                                                    >  SRR19973069.3145186/1‑150 (MQ=60)
  GGACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAA                                                                                                                                                   <  SRR19973069.2217125/150‑1 (MQ=60)
     CGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCT                                                                                                                                                <  SRR19973069.1269273/150‑1 (MQ=60)
      GGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTA                                                                                                                                               <  SRR19973069.2112087/150‑1 (MQ=60)
      GGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGAGTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTA                                                                                                                                               <  SRR19973069.2111328/150‑1 (MQ=60)
       GCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTAC                                                                                                                                              <  SRR19973069.3163494/150‑1 (MQ=60)
       GCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTAC                                                                                                                                              <  SRR19973069.245403/150‑1 (MQ=60)
              CACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGAC                                                                                                                                       >  SRR19973069.3194731/1‑150 (MQ=60)
              CACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGAC                                                                                                                                       >  SRR19973069.1277193/1‑150 (MQ=60)
                   TGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGG                                                                                                                                  >  SRR19973069.3335538/1‑150 (MQ=60)
                   TGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGG                                                                                                                                  >  SRR19973069.2654850/1‑150 (MQ=60)
                       AGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAA                                                                                                                              >  SRR19973069.22344/1‑150 (MQ=60)
                       AGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAA                                                                                                                              >  SRR19973069.22618/1‑150 (MQ=60)
                         AGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAG                                                                                                                            <  SRR19973069.2057006/150‑1 (MQ=60)
                         AGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAG                                                                                                                            >  SRR19973069.254022/1‑150 (MQ=60)
                         AGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAG                                                                                                                            <  SRR19973069.114396/150‑1 (MQ=60)
                         AGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAG                                                                                                                            >  SRR19973069.3382468/1‑150 (MQ=60)
                             CAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCT                                                                                                                        <  SRR19973069.2366312/150‑1 (MQ=60)
                                AGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGC                                                                                                                     >  SRR19973069.3588660/1‑150 (MQ=60)
                                AGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGC                                                                                                                     >  SRR19973069.3588806/1‑150 (MQ=60)
                                  CGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGC                                                                                                                   >  SRR19973069.108371/1‑150 (MQ=60)
                                  CGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGC                                                                                                                   >  SRR19973069.108215/1‑150 (MQ=60)
                                      AAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATAC                                                                                                               >  SRR19973069.3015863/1‑150 (MQ=60)
                                       AACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACG                                                                                                              >  SRR19973069.2458755/1‑150 (MQ=60)
                                          CCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCAC                                                                                                           >  SRR19973069.118369/1‑150 (MQ=60)
                                            GGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAG                                                                                                         >  SRR19973069.740788/1‑150 (MQ=60)
                                            GGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAG                                                                                                         >  SRR19973069.741434/1‑150 (MQ=60)
                                              CAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTA                                                                                                       >  SRR19973069.1274060/1‑150 (MQ=60)
                                              CAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTA                                                                                                       >  SRR19973069.1274082/1‑150 (MQ=60)
                                                ATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATT                                                                                                     >  SRR19973069.913263/1‑150 (MQ=60)
                                                    CGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATG                                                                                                 <  SRR19973069.3751395/150‑1 (MQ=60)
                                                     GGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGC                                                                                                <  SRR19973069.3699897/150‑1 (MQ=60)
                                                      GTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCT                                                                                               <  SRR19973069.2725097/150‑1 (MQ=60)
                                                       TGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTG                                                                                              <  SRR19973069.36797/150‑1 (MQ=60)
                                                       TGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTG                                                                                              <  SRR19973069.1251650/150‑1 (MQ=60)
                                                       TGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTG                                                                                              <  SRR19973069.1251620/150‑1 (MQ=60)
                                                       TGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTG                                                                                              <  SRR19973069.36761/150‑1 (MQ=60)
                                                         CTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCGATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAA                                                                                            >  SRR19973069.2905153/1‑150 (MQ=60)
                                                         CTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCGATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAA                                                                                            >  SRR19973069.739321/1‑150 (MQ=60)
                                                           GATTATGACTGCGTACTCCAGCGTCTAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGAGGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAAC                                                                                          <  SRR19973069.2034068/150‑1 (MQ=60)
                                                           GATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAAC                                                                                          <  SRR19973069.2034076/150‑1 (MQ=60)
                                                            ATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACA                                                                                         >  SRR19973069.3070385/1‑150 (MQ=60)
                                                              TATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACC                                                                                       <  SRR19973069.1977606/150‑1 (MQ=60)
                                                               ATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCT                                                                                      <  SRR19973069.1417006/150‑1 (MQ=60)
                                                                TGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTG                                                                                     <  SRR19973069.3166477/150‑1 (MQ=60)
                                                                TGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTG                                                                                     <  SRR19973069.3274615/150‑1 (MQ=60)
                                                                TGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTG                                                                                     <  SRR19973069.3371368/150‑1 (MQ=60)
                                                                 GACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGC                                                                                    <  SRR19973069.1993481/150‑1 (MQ=60)
                                                                 GACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCCGAAACACCTGC                                                                                    <  SRR19973069.1606479/150‑1 (MQ=60)
                                                                      CGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGA                                                                               >  SRR19973069.3080210/1‑150 (MQ=60)
                                                                      CGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGA                                                                               >  SRR19973069.3080220/1‑150 (MQ=60)
                                                                       GTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGATCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGAC                                                                              <  SRR19973069.563594/150‑1 (MQ=60)
                                                                        TACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACT                                                                             <  SRR19973069.2880757/150‑1 (MQ=60)
                                                                          CTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACCGTATTGATGCTGTAACACCTGCGGTGACTGC                                                                           >  SRR19973069.1333207/1‑150 (MQ=60)
                                                                          CTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGC                                                                           >  SRR19973069.1086536/1‑150 (MQ=60)
                                                                          CTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGC                                                                           >  SRR19973069.1085998/1‑150 (MQ=60)
                                                                           TCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCC                                                                          >  SRR19973069.359374/1‑150 (MQ=60)
                                                                            CCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCA                                                                         >  SRR19973069.3499554/1‑150 (MQ=60)
                                                                            CCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCA                                                                         <  SRR19973069.1363982/150‑1 (MQ=60)
                                                                                CGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCA                                                                     <  SRR19973069.1163036/150‑1 (MQ=60)
                                                                                   CGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTT                                                                  <  SRR19973069.1878441/150‑1 (MQ=60)
                                                                                   CGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTT                                                                  <  SRR19973069.3814361/150‑1 (MQ=60)
                                                                                   CGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTT                                                                  >  SRR19973069.3203725/1‑150 (MQ=60)
                                                                                   CGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTT                                                                  >  SRR19973069.3176198/1‑150 (MQ=60)
                                                                                   CGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTT                                                                  >  SRR19973069.2081964/1‑150 (MQ=60)
                                                                                   CGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTT                                                                  <  SRR19973069.3814428/150‑1 (MQ=60)
                                                                                    GAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTC                                                                 >  SRR19973069.2061478/1‑150 (MQ=60)
                                                                                    GAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTC                                                                 <  SRR19973069.2474194/150‑1 (MQ=60)
                                                                                    GAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTC                                                                 >  SRR19973069.2061508/1‑150 (MQ=60)
                                                                                     AGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCG                                                                <  SRR19973069.842693/150‑1 (MQ=60)
                                                                                             GTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTC                                                        >  SRR19973069.3096586/1‑150 (MQ=60)
                                                                                                 AGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTA                                                    >  SRR19973069.1237792/1‑150 (MQ=60)
                                                                                                    CACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAA                                                 >  SRR19973069.1511744/1‑150 (MQ=60)
                                                                                                     ACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAG                                                <  SRR19973069.1139770/150‑1 (MQ=60)
                                                                                                       tctAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCC                                              <  SRR19973069.2044106/147‑1 (MQ=60)
                                                                                                        GAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCCCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCC                                             <  SRR19973069.2266424/150‑1 (MQ=60)
                                                                                                               GGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATG                                      <  SRR19973069.1563184/150‑1 (MQ=60)
                                                                                                               GGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATG                                      <  SRR19973069.1185292/150‑1 (MQ=60)
                                                                                                               GGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATG                                      <  SRR19973069.2341032/150‑1 (MQ=60)
                                                                                                               GGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATG                                      <  SRR19973069.2341437/150‑1 (MQ=60)
                                                                                                                   CGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAAC                                  >  SRR19973069.2937737/1‑150 (MQ=60)
                                                                                                                     CTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACAC                                >  SRR19973069.1614515/1‑150 (MQ=60)
                                                                                                                     CTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACAC                                >  SRR19973069.3014443/1‑150 (MQ=60)
                                                                                                                     CTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACAC                                >  SRR19973069.3014517/1‑150 (MQ=60)
                                                                                                                     CTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACAC                                >  SRR19973069.3016583/1‑150 (MQ=60)
                                                                                                                      TGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACC                               >  SRR19973069.2907354/1‑150 (MQ=60)
                                                                                                                       GGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCT                              >  SRR19973069.3413812/1‑150 (MQ=60)
                                                                                                                        GATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTG                             <  SRR19973069.3163754/150‑1 (MQ=60)
                                                                                                                        GATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTG                             >  SRR19973069.2921062/1‑150 (MQ=60)
                                                                                                                        GATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTG                             >  SRR19973069.2599880/1‑150 (MQ=60)
                                                                                                                           TATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTC                          >  SRR19973069.3032593/1‑150 (MQ=60)
                                                                                                                            ATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGGATGGTCGGGAAAAGCCCGGCGATGCAACACCTGCTCA                         >  SRR19973069.1374225/1‑150 (MQ=60)
                                                                                                                                CATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGA                     >  SRR19973069.3256284/1‑150 (MQ=60)
                                                                                                                                   CAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAAT                  >  SRR19973069.2471249/1‑150 (MQ=60)
                                                                                                                                   CAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAAT                  >  SRR19973069.3220024/1‑150 (MQ=60)
                                                                                                                                    AAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATC                 >  SRR19973069.3817197/1‑150 (MQ=60)
                                                                                                                                        CGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCC             >  SRR19973069.1208636/1‑150 (MQ=60)
                                                                                                                                         taTGGATTTCTATAACCTACTGGCTACGCTGTAAAAAGCGCTCGCGCATAAGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGACCGGCGATGCAACACCTGCTCAGTGAAATCGCCCT            <  SRR19973069.3717533/148‑1 (MQ=60)
                                                                                                                                         GCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCT            >  SRR19973069.642463/1‑150 (MQ=60)
                                                                                                                                         GCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCT            <  SRR19973069.237346/150‑1 (MQ=60)
                                                                                                                                          CTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTC           >  SRR19973069.3775509/1‑150 (MQ=60)
                                                                                                                                          CTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTC           <  SRR19973069.1939289/150‑1 (MQ=60)
                                                                                                                                            GGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCGT         >  SRR19973069.909038/1‑150 (MQ=60)
                                                                                                                                            GGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCGT         >  SRR19973069.494077/1‑150 (MQ=60)
                                                                                                                                             GATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCGTC        >  SRR19973069.3804742/1‑150 (MQ=60)
                                                                                                                                             GATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCGTC        >  SRR19973069.3804808/1‑150 (MQ=60)
                                                                                                                                              ATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCGTCG       >  SRR19973069.3745507/1‑150 (MQ=60)
                                                                                                                                                  CTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCGTCGCGCC   >  SRR19973069.2207423/1‑150 (MQ=60)
                                                                                                                                                   TATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCGTCGCGCCA  <  SRR19973069.1765121/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
ATGGACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCGATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCGTCGCGCCA  >  CP009273/4193431‑4193727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑