Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F247 I0 R1 1496 299.8 9582018 97.8% 9371213 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 248,447 C→T 86.0% intergenic (+7/‑45) dinB → / → yafN DNA polymerase IV/antitoxin of the YafO‑YafN toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273248,4470CT86.0% 738.9 / 104.5 272intergenic (+7/‑45)dinB/yafNDNA polymerase IV/antitoxin of the YafO‑YafN toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base C (19/19);  new base T (102/132);  total (121/151)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

Only 100 of 285 total aligned reads displayed.

GGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAA  >  CP009273/248301‑248592
                                                                                                                                                  |                                                                                                                                                 
 gCTGAATAAAGCTGATCTTATCTCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTTCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTAtattat                                                                                                                                                <  1:4520525/149‑1 (MQ=255)
 gCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGTGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTAtattat                                                                                                                                                >  1:2630821/1‑149 (MQ=255)
 gCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTAtattat                                                                                                                                                <  2:742536/149‑1 (MQ=255)
 gCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTAtattat                                                                                                                                                >  1:2494217/1‑149 (MQ=255)
    gAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTAtattattat                                                                                                                                             <  1:1455172/149‑1 (MQ=255)
    gAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTAtattattat                                                                                                                                             >  1:4652810/1‑149 (MQ=255)
    gAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTAtattattat                                                                                                                                             >  1:1821839/1‑149 (MQ=255)
    gAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTAtattattat                                                                                                                                             >  1:2077825/1‑149 (MQ=255)
      aTAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGt                                                                                                                                           <  1:857069/149‑1 (MQ=255)
       tAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGta                                                                                                                                          >  2:1301341/1‑149 (MQ=255)
       tAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGta                                                                                                                                          >  2:4255403/1‑149 (MQ=255)
       tAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGta                                                                                                                                          >  2:3196622/1‑149 (MQ=255)
        aaaGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGtat                                                                                                                                         >  2:4075716/1‑149 (MQ=255)
         aaGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGtata                                                                                                                                        >  2:267318/1‑149 (MQ=255)
          aGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTatat                                                                                                                                       >  1:2180614/1‑149 (MQ=255)
            cTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTc                                                                                                                                     <  1:2052233/149‑1 (MQ=255)
            cTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTc                                                                                                                                     <  1:3072817/149‑1 (MQ=255)
             tGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCt                                                                                                                                    <  2:3507048/149‑1 (MQ=255)
               aTCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTgg                                                                                                                                  >  2:2954061/1‑149 (MQ=255)
                  tAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGgtgt                                                                                                                               <  2:3989017/149‑1 (MQ=255)
                  tAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGgtgt                                                                                                                               <  1:3734970/149‑1 (MQ=255)
                   aaTCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGtgtg                                                                                                                              <  1:408902/149‑1 (MQ=255)
                        ccACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCatta                                                                                                                         <  2:4041825/149‑1 (MQ=255)
                        ccACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCatta                                                                                                                         <  1:1382806/149‑1 (MQ=255)
                        ccACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCatta                                                                                                                         <  1:495582/149‑1 (MQ=255)
                         cACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCattat                                                                                                                        >  1:4295409/1‑149 (MQ=255)
                          aCCGCGCGTAACACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCattatt                                                                                                                       <  2:3416993/149‑1 (MQ=255)
                          aCCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCattatt                                                                                                                       <  1:366530/149‑1 (MQ=255)
                          aCCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCattatt                                                                                                                       >  1:654983/1‑149 (MQ=255)
                          aCCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCattatt                                                                                                                       >  1:4185992/1‑149 (MQ=255)
                           ccGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCAttatta                                                                                                                      <  1:1869751/149‑1 (MQ=255)
                            cgcgcgTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATtattat                                                                                                                     <  1:2120990/149‑1 (MQ=255)
                             gcgcgTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATg                                                                                                                    >  1:1925722/1‑149 (MQ=255)
                                 gTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAggg                                                                                                                >  2:1303823/1‑149 (MQ=255)
                                    aaaCCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTAt                                                                                                             <  2:4545212/149‑1 (MQ=255)
                                    aaaCCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTAt                                                                                                             >  1:4142146/1‑149 (MQ=255)
                                      aCCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTAtaa                                                                                                           >  1:2756281/1‑147 (MQ=255)
                                          gggATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGt                                                                                                       >  1:3962352/1‑149 (MQ=255)
                                          gagATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGt                                                                                                       >  2:2295900/3‑149 (MQ=255)
                                           ggATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTa                                                                                                      >  2:1153964/1‑149 (MQ=255)
                                            gATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTAt                                                                                                     >  2:85335/1‑149 (MQ=255)
                                              tGAACGCCGCGGCTGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCGTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGc                                                                                                   <  2:3460233/149‑1 (MQ=255)
                                              tGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGc                                                                                                   <  2:1071094/149‑1 (MQ=255)
                                              tGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGc                                                                                                   >  1:1725357/1‑149 (MQ=255)
                                                aaCGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCAt                                                                                                 >  1:3778912/1‑149 (MQ=255)
                                                    ccgcGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGaa                                                                                             <  2:1026746/149‑1 (MQ=255)
                                                     cgcgGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAAt                                                                                            <  1:343401/149‑1 (MQ=255)
                                                      gcggcgGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAAtt                                                                                           >  2:2430705/1‑149 (MQ=255)
                                                        ggcgggcgCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATtct                                                                                         <  2:3422706/149‑1 (MQ=255)
                                                         gcgggcgCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATtctc                                                                                        >  2:3889067/1‑149 (MQ=255)
                                                         gcgggcgCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATtctc                                                                                        <  1:1055544/149‑1 (MQ=255)
                                                           gggcgCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGc                                                                                      <  1:1594778/149‑1 (MQ=255)
                                                            ggcgCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCt                                                                                     <  2:145061/149‑1 (MQ=255)
                                                             gcgcGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTg                                                                                    <  2:381867/149‑1 (MQ=255)
                                                                  gtgtGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGaaaaa                                                                               <  2:543262/149‑1 (MQ=255)
                                                                   tgtgCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAAt                                                                              <  1:1036134/149‑1 (MQ=255)
                                                                   tgtgCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAAt                                                                              >  2:2690328/1‑149 (MQ=255)
                                                                   tgtgCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAAt                                                                              >  2:1355784/1‑149 (MQ=255)
                                                                    gtgCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATc                                                                             <  2:1905595/149‑1 (MQ=255)
                                                                    gtgCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATc                                                                             <  1:3317124/149‑1 (MQ=255)
                                                                       cGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGt                                                                          <  2:2127284/149‑1 (MQ=255)
                                                                        gTCTGGTGGGGCTGCATGTGACGGTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTc                                                                         <  2:570042/149‑1 (MQ=255)
                                                                        gTCTGGGGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTc                                                                         <  2:2144382/149‑1 (MQ=255)
                                                                             gtggGGCTGCATGTGACGGTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAatat                                                                    <  1:322659/149‑1 (MQ=255)
                                                                                gggCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCAc                                                                 <  1:2876746/149‑1 (MQ=255)
                                                                                   cTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGa                                                              <  2:4759992/149‑1 (MQ=255)
                                                                                     gCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGt                                                            <  1:4350245/149‑1 (MQ=255)
                                                                                      cATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGtt                                                           <  1:3182290/149‑1 (MQ=255)
                                                                                      cATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGtt                                                           <  1:2964118/149‑1 (MQ=255)
                                                                                                 gCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAAcc                                                >  1:1940167/1‑149 (MQ=255)
                                                                                                   ttGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCa                                              <  2:174155/149‑1 (MQ=255)
                                                                                                   ttGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCa                                              <  2:479325/149‑1 (MQ=255)
                                                                                                    tGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAg                                             >  1:519929/1‑149 (MQ=255)
                                                                                                     gACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGc                                            <  2:3553937/149‑1 (MQ=255)
                                                                                                      aCCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCt                                           <  2:3150741/149‑1 (MQ=255)
                                                                                                         ctcAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTaaa                                        <  2:1591299/147‑1 (MQ=255)
                                                                                                         cGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTaaa                                        >  1:803753/1‑149 (MQ=255)
                                                                                                          gaaaaTGGAAAGACAACTGGTGCTGTGTTTATGTTGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGTGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAAt                                       <  1:1764660/147‑1 (MQ=255)
                                                                                                           cAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATa                                      >  1:3407180/1‑149 (MQ=255)
                                                                                                           cAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATa                                      <  2:2631542/149‑1 (MQ=255)
                                                                                                            aaaTGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATAc                                     <  2:2207290/149‑1 (MQ=255)
                                                                                                               tGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACttt                                  <  2:1012860/149‑1 (MQ=255)
                                                                                                                    aGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGa                             <  1:4255403/149‑1 (MQ=255)
                                                                                                                     gACTACTGGTGCTGGGATTATGATGTATACTAGTATGTATATTCTGGTGTGCATTATTATGAGGGGATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGAt                            <  2:3749430/149‑1 (MQ=255)
                                                                                                                     gACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGAt                            <  2:3033233/149‑1 (MQ=255)
                                                                                                                       cAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCa                          <  2:2024610/149‑1 (MQ=255)
                                                                                                                             gTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGt                    >  2:279492/1‑149 (MQ=255)
                                                                                                                             gTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGt                    >  2:3243144/1‑149 (MQ=255)
                                                                                                                                cTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGc                 >  2:4176459/1‑149 (MQ=255)
                                                                                                                                 tgttaTTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCTCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCg                <  2:1550856/145‑1 (MQ=255)
                                                                                                                                  gggATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCgg               >  1:2344720/1‑149 (MQ=255)
                                                                                                                                  gggATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCgg               >  1:458713/1‑149 (MQ=255)
                                                                                                                                      ttATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGttct           >  2:2540416/1‑149 (MQ=255)
                                                                                                                                      ttATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGttct           >  2:798741/1‑149 (MQ=255)
                                                                                                                                      ttATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGttct           <  1:1153964/149‑1 (MQ=255)
                                                                                                                                        atgatgTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGttcttt         <  1:2524164/149‑1 (MQ=255)
                                                                                                                                        atgatgTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGttcttt         >  2:4301405/1‑149 (MQ=255)
                                                                                                                                          gatgTATATGATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGttctttct       <  2:3010500/149‑1 (MQ=255)
                                                                                                                                           atgTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCta      >  1:3403038/1‑149 (MQ=255)
                                                                                                                                              tataTTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCtaata   <  1:284794/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAA  >  CP009273/248301‑248592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGC  >  CP009273/248300‑248596
                                                                                                                                                   |                                                                                                                                                     
CGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTA                                                                                                                                                     >  SRR19973071.1894983/1‑150 (MQ=60)
 GGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGTGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATCGAAAGACAACTGGTGCTGGGATTATGATGTATATTAT                                                                                                                                                    >  SRR19973071.2640297/1‑150 (MQ=60)
 GGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTAT                                                                                                                                                    >  SRR19973071.2503296/1‑150 (MQ=60)
  GCTGAATAAAGCTGATCTTATCTCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTTCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATT                                                                                                                                                   <  SRR19973071.4536618/150‑1 (MQ=60)
  GCTGAATAAAGCTGATCTACTCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATT                                                                                                                                                   <  SRR19973071.2129821/150‑1 (MQ=60)
    TGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTAT                                                                                                                                                 >  SRR19973071.2085702/1‑150 (MQ=60)
    TGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTAT                                                                                                                                                 >  SRR19973071.2085480/1‑150 (MQ=60)
    TGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTAT                                                                                                                                                 >  SRR19973071.1829929/1‑150 (MQ=60)
    TGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTAT                                                                                                                                                 >  SRR19973071.1828619/1‑150 (MQ=60)
    TGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTAT                                                                                                                                                 >  SRR19973071.4669380/1‑150 (MQ=60)
    TGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTAT                                                                                                                                                 <  SRR19973071.825951/150‑1 (MQ=60)
     GAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATG                                                                                                                                                <  SRR19973071.1460495/150‑1 (MQ=60)
       ATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTA                                                                                                                                              <  SRR19973071.860121/150‑1 (MQ=60)
        TAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTAT                                                                                                                                             >  SRR19973071.164578/1‑150 (MQ=60)
        TAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTAT                                                                                                                                             >  SRR19973071.164541/1‑150 (MQ=60)
          AAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTCTATTATTATGTATAT                                                                                                                                           >  SRR19973071.2188670/1‑150 (MQ=60)
          AAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATAT                                                                                                                                           >  SRR19973071.2068723/1‑150 (MQ=60)
          AAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATAT                                                                                                                                           >  SRR19973071.2188677/1‑150 (MQ=60)
          AAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATAT                                                                                                                                           >  SRR19973071.2189094/1‑150 (MQ=60)
          AAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATAT                                                                                                                                           >  SRR19973071.3079836/1‑150 (MQ=60)
             CTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCT                                                                                                                                        <  SRR19973071.3083700/150‑1 (MQ=60)
             CTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCT                                                                                                                                        <  SRR19973071.2059767/150‑1 (MQ=60)
              TGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTG                                                                                                                                       <  SRR19973071.1154410/150‑1 (MQ=60)
                ATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGT                                                                                                                                     >  SRR19973071.3433567/1‑150 (MQ=60)
                 TCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTG                                                                                                                                    <  SRR19973071.2518433/150‑1 (MQ=60)
                 TCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTG                                                                                                                                    <  SRR19973071.2518534/150‑1 (MQ=60)
                   TAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTG                                                                                                                                  >  SRR19973071.3706829/1‑150 (MQ=60)
                   TAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTG                                                                                                                                  <  SRR19973071.3748222/150‑1 (MQ=60)
                    AATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGC                                                                                                                                 <  SRR19973071.3865038/150‑1 (MQ=60)
                    AATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGC                                                                                                                                 <  SRR19973071.410226/150‑1 (MQ=60)
                         CCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTAT                                                                                                                            >  SRR19973071.4310610/1‑150 (MQ=60)
                         CCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTAT                                                                                                                            <  SRR19973071.1387901/150‑1 (MQ=60)
                         CCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTAT                                                                                                                            <  SRR19973071.497424/150‑1 (MQ=60)
                          CACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATT                                                                                                                           >  SRR19973071.657368/1‑150 (MQ=60)
                          CACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATT                                                                                                                           <  SRR19973071.2050313/150‑1 (MQ=60)
                          CACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATT                                                                                                                           >  SRR19973071.656921/1‑150 (MQ=60)
                          CACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATT                                                                                                                           <  SRR19973071.2730877/150‑1 (MQ=60)
                          CACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATT                                                                                                                           >  SRR19973071.4200885/1‑150 (MQ=60)
                          CACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATT                                                                                                                           >  SRR19973071.4200887/1‑150 (MQ=60)
                           ACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTTGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTA                                                                                                                          <  SRR19973071.1350596/150‑1 (MQ=60)
                           ACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTA                                                                                                                          <  SRR19973071.367702/150‑1 (MQ=60)
                           ACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTA                                                                                                                          <  SRR19973071.366983/150‑1 (MQ=60)
                           ACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTA                                                                                                                          >  SRR19973071.556415/1‑150 (MQ=60)
                            CCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTAT                                                                                                                         <  SRR19973071.1876646/150‑1 (MQ=60)
                            CCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTAT                                                                                                                         <  SRR19973071.3060597/150‑1 (MQ=60)
                             CGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATG                                                                                                                        <  SRR19973071.2128823/150‑1 (MQ=60)
                             CGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATG                                                                                                                        >  SRR19973071.1932753/1‑150 (MQ=60)
                             CGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATG                                                                                                                        <  SRR19973071.4637025/150‑1 (MQ=60)
                                GCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGG                                                                                                                     <  SRR19973071.3789135/150‑1 (MQ=60)
                                  GTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGT                                                                                                                   <  SRR19973071.3756051/150‑1 (MQ=60)
                                    AAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTAT                                                                                                                 >  SRR19973071.4156891/1‑150 (MQ=60)
                                     AAACCTGGGCTGCACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATA                                                                                                                <  SRR19973071.4095659/150‑1 (MQ=60)
                                      AACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAA                                                                                                               >  SRR19973071.2766022/1‑150 (MQ=60)
                                        CCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACT                                                                                                             >  SRR19973071.33109/1‑150 (MQ=60)
                                          TGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGT                                                                                                           >  SRR19973071.3976253/1‑150 (MQ=60)
                                           GGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTA                                                                                                          <  SRR19973071.3101704/150‑1 (MQ=60)
                                            GGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTAT                                                                                                         <  SRR19973071.1229478/150‑1 (MQ=60)
                                              ATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGC                                                                                                       >  SRR19973071.1829252/1‑150 (MQ=60)
                                              ATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGC                                                                                                       >  SRR19973071.1731777/1‑150 (MQ=60)
                                              ATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGC                                                                                                       >  SRR19973071.1144042/1‑150 (MQ=60)
                                               TGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCA                                                                                                      <  SRR19973071.2078135/150‑1 (MQ=60)
                                               TGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCA                                                                                                      <  SRR19973071.1594927/150‑1 (MQ=60)
                                               TGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGAA                                                                                                      >  SRR19973071.4304887/1‑150 (MQ=60)
                                                GAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCAT                                                                                                     >  SRR19973071.3792340/1‑150 (MQ=60)
                                                 AACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATC                                                                                                    <  SRR19973071.2872084/150‑1 (MQ=60)
                                                  ACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCG                                                                                                   <  SRR19973071.2630599/150‑1 (MQ=60)
                                                      CGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTCTAACTGTATGCATCGAATT                                                                                               >  SRR19973071.863717/1‑150 (MQ=60)
                                                      CGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATT                                                                                               >  SRR19973071.863778/1‑150 (MQ=60)
                                                      CGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATT                                                                                               <  SRR19973071.344534/150‑1 (MQ=60)
                                                        CGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCT                                                                                             >  SRR19973071.3591646/1‑150 (MQ=60)
                                                          GCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCG                                                                                           <  SRR19973071.1059316/150‑1 (MQ=60)
                                                          GCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCG                                                                                           <  SRR19973071.1059327/150‑1 (MQ=60)
                                                            GGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCT                                                                                         <  SRR19973071.1600406/150‑1 (MQ=60)
                                                            GGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCT                                                                                         <  SRR19973071.3218802/150‑1 (MQ=60)
                                                                   GTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAAT                                                                                  <  SRR19973071.4313216/150‑1 (MQ=60)
                                                                   GTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAAT                                                                                  >  SRR19973071.1426196/1‑150 (MQ=60)
                                                                    TGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATC                                                                                 <  SRR19973071.1039873/150‑1 (MQ=60)
                                                                     GTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCG                                                                                <  SRR19973071.3328886/150‑1 (MQ=60)
                                                                         GTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCA                                                                            <  SRR19973071.2837721/150‑1 (MQ=60)
                                                                         GTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCA                                                                            >  SRR19973071.3363542/1‑150 (MQ=60)
                                                                          TCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAA                                                                           <  SRR19973071.4243501/150‑1 (MQ=60)
                                                                             GGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATAT                                                                        <  SRR19973071.341628/150‑1 (MQ=60)
                                                                              GTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATC                                                                       >  SRR19973071.2501625/1‑150 (MQ=60)
                                                                              GTGGGGCTGCATGTGACGGTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATC                                                                       <  SRR19973071.323761/150‑1 (MQ=60)
                                                                                GGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCAC                                                                     >  SRR19973071.4320740/1‑150 (MQ=60)
                                                                                 GGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACT                                                                    <  SRR19973071.2887009/150‑1 (MQ=60)
                                                                                 GGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACT                                                                    >  SRR19973071.4125160/1‑150 (MQ=60)
                                                                                 GGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGAGTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACT                                                                    >  SRR19973071.4125010/1‑150 (MQ=60)
                                                                                      GCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTT                                                               <  SRR19973071.4365573/150‑1 (MQ=60)
                                                                                       CATGTGACGTTGCTTGACCCGCACATCGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTA                                                              <  SRR19973071.343915/150‑1 (MQ=60)
                                                                                       CATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTA                                                              <  SRR19973071.2974730/150‑1 (MQ=60)
                                                                                       CATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTA                                                              <  SRR19973071.2974441/150‑1 (MQ=60)
                                                                                       CATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTA                                                              <  SRR19973071.3193368/150‑1 (MQ=60)
                                                                                          GTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGT                                                           >  SRR19973071.50383/1‑150 (MQ=60)
                                                                                              CGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAA                                                       <  SRR19973071.2483372/150‑1 (MQ=60)
                                                                                              CGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAA                                                       <  SRR19973071.3194042/150‑1 (MQ=60)
                                                                                                 TGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACC                                                    >  SRR19973071.1947257/1‑150 (MQ=60)
                                                                                                 TGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACC                                                    >  SRR19973071.1947234/1‑150 (MQ=60)
                                                                                                 TGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACC                                                    >  SRR19973071.1947218/1‑150 (MQ=60)
                                                                                                 TGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACC                                                    >  SRR19973071.1946939/1‑150 (MQ=60)
                                                                                                    TTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAG                                                 >  SRR19973071.2603229/1‑150 (MQ=60)
                                                                                                    TTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAG                                                 >  SRR19973071.521878/1‑150 (MQ=60)
                                                                                                    TTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAG                                                 >  SRR19973071.3709608/1‑150 (MQ=60)
                                                                                                     TGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGC                                                <  SRR19973071.1769934/150‑1 (MQ=60)
                                                                                                         CCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAA                                            <  SRR19973071.2971815/150‑1 (MQ=60)
                                                                                                         CCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAA                                            >  SRR19973071.806428/1‑150 (MQ=60)
                                                                                                          CGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAAT                                           <  SRR19973071.4608991/150‑1 (MQ=60)
                                                                                                           GCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATA                                          >  SRR19973071.3419242/1‑150 (MQ=60)
                                                                                                           GCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATA                                          <  SRR19973071.1579989/150‑1 (MQ=60)
                                                                                                           GAAAATGGAAAGACAACTGGTGCTGTGTTTATGTTGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGTGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATA                                          <  SRR19973071.1771249/150‑1 (MQ=60)
                                                                                                             AAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACT                                        >  SRR19973071.4172994/1‑150 (MQ=60)
                                                                                                                TGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTA                                     <  SRR19973071.4603094/150‑1 (MQ=60)
                                                                                                                     AGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGAT                                <  SRR19973071.4270502/150‑1 (MQ=60)
                                                                                                                       ACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCA                              >  SRR19973071.4730102/1‑150 (MQ=60)
                                                                                                                       ACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCA                              >  SRR19973071.705876/1‑150 (MQ=60)
                                                                                                                           CTGGTTCTGTGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCG                          <  SRR19973071.854580/150‑1 (MQ=60)
                                                                                                                            TGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGG                         <  SRR19973071.2663575/150‑1 (MQ=60)
                                                                                                                             GGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGT                        <  SRR19973071.1925671/150‑1 (MQ=60)
                                                                                                                              GTTCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCTGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCTGTT                       <  SRR19973071.3377701/150‑1 (MQ=60)
                                                                                                                                  TGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGG                   >  SRR19973071.2353233/1‑150 (MQ=60)
                                                                                                                                  TGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGG                   >  SRR19973071.2240927/1‑150 (MQ=60)
                                                                                                                                  TGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGG                   >  SRR19973071.460375/1‑150 (MQ=60)
                                                                                                                                  TGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGG                   >  SRR19973071.460387/1‑150 (MQ=60)
                                                                                                                                  TGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGG                   <  SRR19973071.4297665/150‑1 (MQ=60)
                                                                                                                                   GGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGT                  >  SRR19973071.437829/1‑150 (MQ=60)
                                                                                                                                       TTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTT              <  SRR19973071.1157975/150‑1 (MQ=60)
                                                                                                                                        TATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTT             >  SRR19973071.4013937/1‑150 (MQ=60)
                                                                                                                                         ATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTC            <  SRR19973071.2533351/150‑1 (MQ=60)
                                                                                                                                         ATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTC            >  SRR19973071.3168780/1‑150 (MQ=60)
                                                                                                                                          TGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCT           >  SRR19973071.1786319/1‑150 (MQ=60)
                                                                                                                                          TGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCT           >  SRR19973071.4737887/1‑150 (MQ=60)
                                                                                                                                           GATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTA          >  SRR19973071.3415144/1‑150 (MQ=60)
                                                                                                                                           GATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTA          >  SRR19973071.3415091/1‑150 (MQ=60)
                                                                                                                                           GATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTA          >  SRR19973071.4242783/1‑150 (MQ=60)
                                                                                                                                           GATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTA          <  SRR19973071.1799402/150‑1 (MQ=60)
                                                                                                                                             TGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAAT        >  SRR19973071.142772/1‑150 (MQ=60)
                                                                                                                                              GTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATA       <  SRR19973071.499419/150‑1 (MQ=60)
                                                                                                                                              GTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATA       >  SRR19973071.2869105/1‑150 (MQ=60)
                                                                                                                                               TATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAA      <  SRR19973071.285829/150‑1 (MQ=60)
                                                                                                                                                 TATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATC    <  SRR19973071.2218480/150‑1 (MQ=60)
                                                                                                                                                 TATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATC    <  SRR19973071.3023335/150‑1 (MQ=60)
                                                                                                                                                   CTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGC  >  SRR19973071.1133442/1‑150 (MQ=60)
                                                                                                                                                   CTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGC  >  SRR19973071.1133443/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
CGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGC  >  CP009273/248300‑248596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑