Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 248,447 C→T 100% intergenic (+7/‑45) dinB → / → yafN DNA polymerase IV/antitoxin of the YafO‑YafN toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273248,4470CT97.8% 743.2 / 3.9 231intergenic (+7/‑45)dinB/yafNDNA polymerase IV/antitoxin of the YafO‑YafN toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base C (1/4);  new base T (84/141);  total (86/145)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 238 total aligned reads displayed.

GGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATA  >  CP009273/248301‑248591
                                                                                                                                                  |                                                                                                                                                
ggCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTAtattat                                                                                                                                               >  1:4602003/1‑150 (MQ=255)
ggCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTAtattat                                                                                                                                               >  1:4580045/1‑150 (MQ=255)
  cTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATAttatta                                                                                                                                             >  2:4576200/1‑150 (MQ=255)
  cTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATAttatta                                                                                                                                             >  1:3539532/1‑150 (MQ=255)
   tGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGATTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTAtattattat                                                                                                                                            >  2:1178663/1‑150 (MQ=255)
    gAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATg                                                                                                                                           >  2:3718108/1‑150 (MQ=255)
    gAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATg                                                                                                                                           >  1:1656440/1‑150 (MQ=255)
           gCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTc                                                                                                                                    <  2:2780011/150‑1 (MQ=255)
                tCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCACATGGCAAGACCACTGGTGCTGGGATTATGCGGTATATTAGTATGTATCTTCggggg                                                                                                                               >  2:4369665/1‑148 (MQ=255)
                 cTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGgtgt                                                                                                                              <  2:2421637/150‑1 (MQ=255)
                  tAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACCACTGGTGCTGGGATTATGATGTATATTATGATGTATATTCTGGtgtg                                                                                                                             >  2:66714/1‑150 (MQ=255)
                   aaTCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGc                                                                                                                            <  2:1369483/150‑1 (MQ=255)
                   aaTCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGc                                                                                                                            <  1:707562/150‑1 (MQ=255)
                    aTCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCa                                                                                                                           >  1:990195/1‑150 (MQ=255)
                     tCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCat                                                                                                                          >  1:4049240/1‑150 (MQ=255)
                      cGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCatt                                                                                                                         >  1:1465698/1‑150 (MQ=255)
                        ccACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCattat                                                                                                                       <  1:1336986/150‑1 (MQ=255)
                        ccACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCattat                                                                                                                       >  1:2701898/1‑150 (MQ=255)
                         cACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCattatt                                                                                                                      <  2:4946066/150‑1 (MQ=255)
                             gcgcgTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGa                                                                                                                  >  1:1179032/1‑150 (MQ=255)
                              cgcgTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAg                                                                                                                 <  1:2819352/150‑1 (MQ=255)
                               gcgTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAgg                                                                                                                <  2:2386441/150‑1 (MQ=255)
                                cgTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAggg                                                                                                               >  2:1724428/1‑150 (MQ=255)
                                 gTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGt                                                                                                              >  1:3807707/1‑150 (MQ=255)
                                 gTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGt                                                                                                              >  2:2804870/1‑150 (MQ=255)
                                 gTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGt                                                                                                              >  2:2804725/1‑150 (MQ=255)
                                   aaaaCCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTAt                                                                                                            <  1:2843098/150‑1 (MQ=255)
                                        cTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTCTAACTg                                                                                                       >  2:3453337/1‑150 (MQ=255)
                                        cTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTg                                                                                                       >  1:3128241/1‑150 (MQ=255)
                                          gggATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTa                                                                                                     >  1:3143640/1‑150 (MQ=255)
                                          gggATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTa                                                                                                     <  1:2360152/150‑1 (MQ=255)
                                             aTGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGGTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGc                                                                                                  <  2:2727318/150‑1 (MQ=255)
                                                aaCGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATc                                                                                               <  1:4621825/150‑1 (MQ=255)
                                                  cgccgcGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGa                                                                                             <  2:1836016/150‑1 (MQ=255)
                                                   gccgcGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGaa                                                                                            <  2:2234264/150‑1 (MQ=255)
                                                   gccgcGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGaa                                                                                            >  1:219819/1‑150 (MQ=255)
                                                     cgcgGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAAtt                                                                                          <  2:2503274/150‑1 (MQ=255)
                                                       cggcggGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATtct                                                                                        <  2:5069644/150‑1 (MQ=255)
                                                        ggcgggcgCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATtctc                                                                                       >  1:3694637/1‑150 (MQ=255)
                                                          cgggcgCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGc                                                                                     <  1:1018024/150‑1 (MQ=255)
                                                           gggcgCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCt                                                                                    <  2:842317/150‑1 (MQ=255)
                                                           gggcgCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCt                                                                                    <  2:4669969/150‑1 (MQ=255)
                                                             gcgcGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGa                                                                                  <  1:4956101/150‑1 (MQ=255)
                                                                    gtgCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCg                                                                           <  2:2006971/150‑1 (MQ=255)
                                                                     tgCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCgg                                                                          >  2:1538017/1‑150 (MQ=255)
                                                                      gCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGt                                                                         <  1:3180560/150‑1 (MQ=255)
                                                                      gCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGt                                                                         <  1:2965008/150‑1 (MQ=255)
                                                                       cGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTc                                                                        <  1:2028859/150‑1 (MQ=255)
                                                                           tggtggGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAata                                                                    >  2:3077955/1‑150 (MQ=255)
                                                                              tggGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCa                                                                 >  1:4025093/1‑150 (MQ=255)
                                                                               ggggCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCAc                                                                <  1:2268490/150‑1 (MQ=255)
                                                                                gggCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACt                                                               >  1:543803/1‑150 (MQ=255)
                                                                                 ggCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTg                                                              >  1:4914837/1‑150 (MQ=255)
                                                                                   cTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAg                                                            <  2:1436811/150‑1 (MQ=255)
                                                                                   cTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAg                                                            >  2:2682004/1‑150 (MQ=255)
                                                                                     gCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGtt                                                          <  1:2815054/150‑1 (MQ=255)
                                                                                     gCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGtt                                                          <  1:4755214/150‑1 (MQ=255)
                                                                                      cATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTa                                                         <  2:786989/150‑1 (MQ=255)
                                                                                       aTGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTAc                                                        <  1:3357625/150‑1 (MQ=255)
                                                                                       aTGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTAc                                                        <  2:131834/150‑1 (MQ=255)
                                                                                       aTGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTAc                                                        <  2:4528762/150‑1 (MQ=255)
                                                                                         gtgACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGt                                                      >  2:4165145/1‑150 (MQ=255)
                                                                                          tgACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTa                                                     <  1:4571579/150‑1 (MQ=255)
                                                                                           gACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTaa                                                    <  2:2539542/150‑1 (MQ=255)
                                                                                                 gCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAAccc                                              <  1:2184027/150‑1 (MQ=255)
                                                                                                  cTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCa                                             <  1:4863773/150‑1 (MQ=255)
                                                                                                  cTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCa                                             <  2:4602003/150‑1 (MQ=255)
                                                                                                  cTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCa                                             <  1:5131635/150‑1 (MQ=255)
                                                                                                   ttGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAg                                            >  2:1236504/1‑150 (MQ=255)
                                                                                                    tGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGc                                           <  2:3053600/150‑1 (MQ=255)
                                                                                                    tGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGc                                           <  2:3056868/150‑1 (MQ=255)
                                                                                                     gACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCt                                          <  1:1555896/150‑1 (MQ=255)
                                                                                                         cGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAAt                                      <  2:4225124/150‑1 (MQ=255)
                                                                                                          gCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATa                                     <  2:4063821/150‑1 (MQ=255)
                                                                                                           cAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATAc                                    <  2:4508156/150‑1 (MQ=255)
                                                                                                            aaaTGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACt                                   >  1:477315/1‑150 (MQ=255)
                                                                                                              aTGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCATTGAGTTACGTAAAAACCCAGCTAAATACttt                                 >  1:4240084/1‑150 (MQ=255)
                                                                                                               tGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTa                                >  2:187827/1‑150 (MQ=255)
                                                                                                               tGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTa                                >  2:1488318/1‑150 (MQ=255)
                                                                                                                ggAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTAt                               <  2:1526447/150‑1 (MQ=255)
                                                                                                                    aGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGAt                           >  1:3408145/1‑150 (MQ=255)
                                                                                                                     gACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATc                          <  2:3116753/150‑1 (MQ=255)
                                                                                                                      aCAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCa                         <  1:1714187/150‑1 (MQ=255)
                                                                                                                      aCAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCa                         <  2:4985434/150‑1 (MQ=255)
                                                                                                                       cAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCaa                        <  2:2736894/150‑1 (MQ=255)
                                                                                                                         aCTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAAcc                      <  1:36555/150‑1 (MQ=255)
                                                                                                                         aCTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAAcc                      <  1:1042641/150‑1 (MQ=255)
                                                                                                                          cTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCATTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCg                     <  2:4307069/150‑1 (MQ=255)
                                                                                                                          cTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAAcca                     <  2:3252354/150‑2 (MQ=255)
                                                                                                                            ggTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGt                   <  1:4986025/150‑1 (MQ=255)
                                                                                                                             gTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGtt                  >  1:4283083/1‑150 (MQ=255)
                                                                                                                             gTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGtt                  <  1:3034446/150‑1 (MQ=255)
                                                                                                                              tGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTg                 <  2:3572458/150‑1 (MQ=255)
                                                                                                                                 tGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCgg              <  2:4885294/150‑1 (MQ=255)
                                                                                                                                   ggATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGtt            <  1:2312749/150‑1 (MQ=255)
                                                                                                                                     aTTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGttct          <  2:3844399/150‑1 (MQ=255)
                                                                                                                                       tATGATGTATATTATTATGTATTTTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGttcttt        <  2:4739550/150‑1 (MQ=255)
                                                                                                                                        atgatgTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGttctttc       <  2:3908521/150‑1 (MQ=255)
                                                                                                                                          gatgTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCta     <  1:4489325/150‑1 (MQ=255)
                                                                                                                                            tgTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCtaat   <  1:2565832/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATA  >  CP009273/248301‑248591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCG  >  CP009273/248298‑248595
                                                                                                                                                     |                                                                                                                                                    
CGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATAT                                                                                                                                                      >  SRR19973063.3947021/1‑150 (MQ=60)
CGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATAT                                                                                                                                                      <  SRR19973063.5060731/150‑1 (MQ=60)
CGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATAT                                                                                                                                                      <  SRR19973063.4447328/150‑1 (MQ=60)
CGCGGCTGAATAAAGCTGATCTAATCGCCACAGCGCGTAAAACATGGGATGAACTCCTCGGAGGGCGCGGAGTGCGTCTGGTGGGGCTGCATGTGACTTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATAT                                                                                                                                                      >  SRR19973063.1276836/1‑150 (MQ=60)
 GCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATT                                                                                                                                                     >  SRR19973063.793402/1‑150 (MQ=60)
 GCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATT                                                                                                                                                     >  SRR19973063.3076431/1‑150 (MQ=60)
 GCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATT                                                                                                                                                     >  SRR19973063.2277561/1‑150 (MQ=60)
   GGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTAT                                                                                                                                                   >  SRR19973063.4594697/1‑150 (MQ=60)
   GGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTAT                                                                                                                                                   >  SRR19973063.4594690/1‑150 (MQ=60)
   GGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTAT                                                                                                                                                   >  SRR19973063.4616725/1‑150 (MQ=60)
     CTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTA                                                                                                                                                 >  SRR19973063.3550872/1‑150 (MQ=60)
      TGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTAT                                                                                                                                                >  SRR19973063.4784603/1‑150 (MQ=60)
       GAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATG                                                                                                                                               >  SRR19973063.1661491/1‑150 (MQ=60)
           AAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATA                                                                                                                                           >  SRR19973063.4981324/1‑150 (MQ=60)
            AAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATAT                                                                                                                                          >  SRR19973063.4603471/1‑150 (MQ=60)
               CTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCT                                                                                                                                       >  SRR19973063.2046670/1‑150 (MQ=60)
                   TCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTG                                                                                                                                   <  SRR19973063.227588/150‑1 (MQ=60)
                   TCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTG                                                                                                                                   <  SRR19973063.2830049/150‑1 (MQ=60)
                     TAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTG                                                                                                                                 <  SRR19973063.4822339/150‑1 (MQ=60)
                     TAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTG                                                                                                                                 >  SRR19973063.282618/1‑150 (MQ=60)
                      AATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGC                                                                                                                                <  SRR19973063.709641/150‑1 (MQ=60)
                      AATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGC                                                                                                                                <  SRR19973063.709651/150‑1 (MQ=60)
                       ATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCA                                                                                                                               >  SRR19973063.993183/1‑150 (MQ=60)
                        TCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCAT                                                                                                                              >  SRR19973063.4062215/1‑150 (MQ=60)
                         CGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATT                                                                                                                             >  SRR19973063.1470174/1‑150 (MQ=60)
                           CCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTAT                                                                                                                           <  SRR19973063.173120/150‑1 (MQ=60)
                           CCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTAT                                                                                                                           <  SRR19973063.1340994/150‑1 (MQ=60)
                           CCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTAT                                                                                                                           >  SRR19973063.2710610/1‑150 (MQ=60)
                             ACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTA                                                                                                                         <  SRR19973063.359504/150‑1 (MQ=60)
                                GCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGA                                                                                                                      >  SRR19973063.1182592/1‑150 (MQ=60)
                                 CGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAG                                                                                                                     <  SRR19973063.2828414/150‑1 (MQ=60)
                                    GTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGT                                                                                                                  >  SRR19973063.3820022/1‑150 (MQ=60)
                                    GTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGT                                                                                                                  >  SRR19973063.3819830/1‑150 (MQ=60)
                                      AAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTAT                                                                                                                <  SRR19973063.2852219/150‑1 (MQ=60)
                                      AAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTAT                                                                                                                <  SRR19973063.1031453/150‑1 (MQ=60)
                                      AAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTAT                                                                                                                >  SRR19973063.1608980/1‑150 (MQ=60)
                                           CTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTG                                                                                                           >  SRR19973063.3138311/1‑150 (MQ=60)
                                           CTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTG                                                                                                           >  SRR19973063.4611385/1‑150 (MQ=60)
                                             GGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTA                                                                                                         >  SRR19973063.3153748/1‑150 (MQ=60)
                                             GGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTA                                                                                                         <  SRR19973063.2367710/150‑1 (MQ=60)
                                                ATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGC                                                                                                      <  SRR19973063.257380/150‑1 (MQ=60)
                                                   AACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATC                                                                                                   <  SRR19973063.4636590/150‑1 (MQ=60)
                                                   AACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATC                                                                                                   <  SRR19973063.4640196/150‑1 (MQ=60)
                                                    ACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCG                                                                                                  >  SRR19973063.3734729/1‑150 (MQ=60)
                                                      GCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAA                                                                                                >  SRR19973063.220431/1‑150 (MQ=60)
                                                      GCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAA                                                                                                >  SRR19973063.220480/1‑150 (MQ=60)
                                                      GCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAA                                                                                                >  SRR19973063.220482/1‑150 (MQ=60)
                                                        CGCGGCGGGCGCGGTGTGCGTCTGGTTGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGTTGTGCATTATTATGAGGGTCTAACTGTATGCATCGAATT                                                                                              >  SRR19973063.4625290/1‑150 (MQ=60)
                                                          CGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCT                                                                                            <  SRR19973063.1112141/150‑1 (MQ=60)
                                                          CGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCT                                                                                            <  SRR19973063.3151521/150‑1 (MQ=60)
                                                           GGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTC                                                                                           >  SRR19973063.3706469/1‑150 (MQ=60)
                                                           GGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACGGTATGCATCGAATTCTC                                                                                           <  SRR19973063.29166/150‑1 (MQ=60)
                                                             CGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGC                                                                                         <  SRR19973063.1021092/150‑1 (MQ=60)
                                                               GGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTG                                                                                       <  SRR19973063.3508457/150‑1 (MQ=60)
                                                                GCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGA                                                                                      <  SRR19973063.2403438/150‑1 (MQ=60)
                                                                GCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGA                                                                                      <  SRR19973063.4972124/150‑1 (MQ=60)
                                                                  GCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAA                                                                                    >  SRR19973063.4144369/1‑150 (MQ=60)
                                                                      TGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGTTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATC                                                                                >  SRR19973063.97574/1‑150 (MQ=60)
                                                                       GTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCG                                                                               <  SRR19973063.3147785/150‑1 (MQ=60)
                                                                       GTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCG                                                                               <  SRR19973063.128200/150‑1 (MQ=60)
                                                                       GTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCG                                                                               <  SRR19973063.128490/150‑1 (MQ=60)
                                                                       GTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCG                                                                               >  SRR19973063.3401389/1‑150 (MQ=60)
                                                                         GCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGT                                                                             <  SRR19973063.2974487/150‑1 (MQ=60)
                                                                         GCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGT                                                                             <  SRR19973063.1604105/150‑1 (MQ=60)
                                                                         GCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGT                                                                             <  SRR19973063.3190787/150‑1 (MQ=60)
                                                                          CGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTC                                                                            <  SRR19973063.2035194/150‑1 (MQ=60)
                                                                                GTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATC                                                                      >  SRR19973063.2261676/1‑150 (MQ=60)
                                                                                 TGGGGCTGCATGTGCCGTTGCTAGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCA                                                                     <  SRR19973063.4376989/150‑1 (MQ=60)
                                                                                 TGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCA                                                                     >  SRR19973063.4038024/1‑150 (MQ=60)
                                                                                 TGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCA                                                                     >  SRR19973063.4038002/1‑150 (MQ=60)
                                                                                  GGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCAC                                                                    <  SRR19973063.2275725/150‑1 (MQ=60)
                                                                                   GGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACT                                                                   >  SRR19973063.545411/1‑150 (MQ=60)
                                                                                    GGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTG                                                                  >  SRR19973063.4930657/1‑150 (MQ=60)
                                                                                    GGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTG                                                                  >  SRR19973063.4930709/1‑150 (MQ=60)
                                                                                       TGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGT                                                               >  SRR19973063.5128219/1‑150 (MQ=60)
                                                                                        GCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTT                                                              <  SRR19973063.4770376/150‑1 (MQ=60)
                                                                                        GCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTT                                                              <  SRR19973063.2824104/150‑1 (MQ=60)
                                                                                        GCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTT                                                              <  SRR19973063.4770391/150‑1 (MQ=60)
                                                                                          ATGTGACGTTGCTTGACCCGCACATGGAAAGACAACTGGTGCTGGGATGATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTAC                                                            <  SRR19973063.4947072/150‑1 (MQ=60)
                                                                                          ATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTAC                                                            <  SRR19973063.4564086/150‑1 (MQ=60)
                                                                                          ATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTAC                                                            <  SRR19973063.4564085/150‑1 (MQ=60)
                                                                                          ATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTAC                                                            <  SRR19973063.4961477/150‑1 (MQ=60)
                                                                                          ATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTAC                                                            <  SRR19973063.3368313/150‑1 (MQ=60)
                                                                                             TGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTA                                                         <  SRR19973063.2756740/150‑1 (MQ=60)
                                                                                             TGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTA                                                         <  SRR19973063.4586206/150‑1 (MQ=60)
                                                                                                  TTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAAC                                                    >  SRR19973063.3177372/1‑150 (MQ=60)
                                                                                                  TTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAAC                                                    >  SRR19973063.3271946/1‑150 (MQ=60)
                                                                                                    GCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCC                                                  <  SRR19973063.2190869/150‑1 (MQ=60)
                                                                                                     CTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCA                                                 <  SRR19973063.5148138/150‑1 (MQ=60)
                                                                                                     CTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCA                                                 <  SRR19973063.4879447/150‑1 (MQ=60)
                                                                                                     CTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCA                                                 <  SRR19973063.3009716/150‑1 (MQ=60)
                                                                                                     CTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCA                                                 <  SRR19973063.5179609/150‑1 (MQ=60)
                                                                                                     CTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCA                                                 <  SRR19973063.5148141/150‑1 (MQ=60)
                                                                                                        GACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCT                                              <  SRR19973063.1560662/150‑1 (MQ=60)
                                                                                                         ACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTA                                             <  SRR19973063.4205044/150‑1 (MQ=60)
                                                                                                           CCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAA                                           <  SRR19973063.2751867/150‑1 (MQ=60)
                                                                                                             GCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCATTGAGTTACGTAAAAACCCAGCTAAATA                                         <  SRR19973063.1759561/150‑1 (MQ=60)
                                                                                                               AAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACT                                       <  SRR19973063.3768712/150‑1 (MQ=60)
                                                                                                               AAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACT                                       >  SRR19973063.478668/1‑150 (MQ=60)
                                                                                                                AATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTT                                      <  SRR19973063.1534648/150‑1 (MQ=60)
                                                                                                                 ATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCATTGAGTTACGTAAAAACCCAGCTAAATACTTT                                     >  SRR19973063.4253597/1‑150 (MQ=60)
                                                                                                                 ATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCATTGAGTTACGTAAAAACCCAGCTAAATACTTT                                     >  SRR19973063.4253711/1‑150 (MQ=60)
                                                                                                                     AAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTG                                 >  SRR19973063.627536/1‑150 (MQ=60)
                                                                                                                       AGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGAT                               >  SRR19973063.3418985/1‑150 (MQ=60)
                                                                                                                         ACAACTGGTGCTGGGATTATGATGTATATTATTATGTATGTTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCA                             <  SRR19973063.1719411/150‑1 (MQ=60)
                                                                                                                         ACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCA                             <  SRR19973063.2913405/150‑1 (MQ=60)
                                                                                                                         ACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCA                             <  SRR19973063.1719415/150‑1 (MQ=60)
                                                                                                                           AACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAAC                           >  SRR19973063.1388243/1‑150 (MQ=60)
                                                                                                                            ACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC                          <  SRR19973063.36757/150‑1 (MQ=60)
                                                                                                                            ACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC                          <  SRR19973063.36698/150‑1 (MQ=60)
                                                                                                                            ACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC                          <  SRR19973063.1045798/150‑1 (MQ=60)
                                                                                                                               GGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGT                       <  SRR19973063.5002135/150‑1 (MQ=60)
                                                                                                                                GTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTT                      >  SRR19973063.4296693/1‑150 (MQ=60)
                                                                                                                                GTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTT                      <  SRR19973063.3044139/150‑1 (MQ=60)
                                                                                                                                 TGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTG                     <  SRR19973063.3552076/150‑1 (MQ=60)
                                                                                                                                      GGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTT                <  SRR19973063.2320146/150‑1 (MQ=60)
                                                                                                                                      GGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTT                <  SRR19973063.2320179/150‑1 (MQ=60)
                                                                                                                                         TTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTT             <  SRR19973063.3287181/150‑1 (MQ=60)
                                                                                                                                           ATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCATTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTC           <  SRR19973063.3080162/150‑1 (MQ=60)
                                                                                                                                             GATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTA         <  SRR19973063.4503725/150‑1 (MQ=60)
                                                                                                                                               TGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAAT       <  SRR19973063.2574008/150‑1 (MQ=60)
                                                                                                                                               TGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAAT       <  SRR19973063.2563388/150‑1 (MQ=60)
                                                                                                                                                    ATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCG  <  SRR19973063.1339718/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCG  >  CP009273/248298‑248595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑