Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F247 I0 R1 1496 299.8 9582018 97.8% 9371213 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 372,091 C→A 84.6% R393R (CGG→AGG)  mhpT → 3‑hydroxyphenylpropionic transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273372,0910CA84.6% 518.4 / 110.1 240R393R (CGG→AGG) mhpT3‑hydroxyphenylpropionic transporter
Reads supporting (aligned to +/- strand):  ref base C (22/15);  new base A (93/109);  total (115/125)
Fisher's exact test for biased strand distribution p-value = 1.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.57e-01

Only 100 of 240 total aligned reads displayed.

GCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAA  >  CP009273/371944‑372236
                                                                                                                                                   |                                                                                                                                                 
 ccGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCgg                                                                                                                                                 <  2:1200800/149‑1 (MQ=255)
  cGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCAGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGga                                                                                                                                                >  2:1699864/1‑149 (MQ=255)
      gCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATc                                                                                                                                            <  2:498487/149‑1 (MQ=255)
         gTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACg                                                                                                                                         >  1:667482/1‑149 (MQ=255)
           aGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGaa                                                                                                                                       <  1:3030325/149‑1 (MQ=255)
             ggCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATa                                                                                                                                     <  2:4320594/149‑1 (MQ=255)
                gTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAg                                                                                                                                  >  1:1754415/1‑149 (MQ=255)
                gTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAg                                                                                                                                  <  1:931791/149‑1 (MQ=255)
                gTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAg                                                                                                                                  >  1:1130706/1‑149 (MQ=255)
                 tCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGc                                                                                                                                 <  1:2291885/149‑1 (MQ=255)
                  cTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGcc                                                                                                                                <  1:1197602/149‑1 (MQ=255)
                  cTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGcc                                                                                                                                <  2:2908142/149‑1 (MQ=255)
                      gggCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTgc                                                                                                                            <  1:4431498/149‑1 (MQ=255)
                      gggCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTgc                                                                                                                            <  2:2430065/149‑1 (MQ=255)
                       ggCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTgcg                                                                                                                           <  2:3882969/149‑1 (MQ=255)
                        gCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTgcgc                                                                                                                          >  1:4444528/1‑149 (MQ=255)
                        gCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTgcgc                                                                                                                          >  1:2607876/1‑149 (MQ=255)
                            tGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGAt                                                                                                                      >  1:4013993/1‑149 (MQ=255)
                             gAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATg                                                                                                                     >  2:539480/1‑149 (MQ=255)
                              aGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGc                                                                                                                    <  2:2351850/149‑1 (MQ=255)
                               gCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGcc                                                                                                                   <  1:511878/149‑1 (MQ=255)
                                 ggTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATTAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTg                                                                                                                 >  1:1238754/1‑149 (MQ=255)
                                 ggTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTg                                                                                                                 >  2:2840089/1‑149 (MQ=255)
                                  gTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGa                                                                                                                >  2:3322164/1‑149 (MQ=255)
                                  gTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGa                                                                                                                >  2:3751165/1‑149 (MQ=255)
                                   tCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGAc                                                                                                               >  1:886710/1‑149 (MQ=255)
                                      gTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTg                                                                                                            >  1:2512638/1‑149 (MQ=255)
                                      gTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTg                                                                                                            <  2:785490/149‑1 (MQ=255)
                                       ttACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGc                                                                                                           >  1:2101297/1‑149 (MQ=255)
                                        tACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGcc                                                                                                          >  1:1606266/1‑149 (MQ=255)
                                         aCTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCt                                                                                                         >  1:1340319/1‑149 (MQ=255)
                                           tGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCtta                                                                                                       >  1:2164259/1‑149 (MQ=255)
                                           tGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCtta                                                                                                       <  2:1629936/149‑1 (MQ=255)
                                                gggAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAgg                                                                                                  >  2:3262097/1‑149 (MQ=255)
                                                gggAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAgg                                                                                                  <  2:2569817/149‑1 (MQ=255)
                                                 ggAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCAGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGc                                                                                                 >  2:2202794/1‑149 (MQ=255)
                                                    aaaTGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAgg                                                                                              >  1:3917644/1‑149 (MQ=255)
                                                      aTGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAgggg                                                                                            >  2:4660364/1‑149 (MQ=255)
                                                      aTGCTCGCATTAGGCACTGGCACGGTCGGCGAAATCGCCGCTTCTGCACCGGCTAAACTTGTTGCTGGGTTGGCGGTGTATATTTTGAAGAGCCGGAGATCACGAATACATCCGTGCGCCGAAGCCTGACGTGCCTTATTAGGCAgggg                                                                                            >  2:3659340/1‑149 (MQ=255)
                                                         cTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGa                                                                                         <  1:336660/149‑1 (MQ=255)
                                                          tGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGaa                                                                                        >  2:459988/1‑149 (MQ=255)
                                                           ggCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGaaa                                                                                       <  1:3488934/149‑1 (MQ=255)
                                                           ggCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGaaa                                                                                       <  1:1894963/149‑1 (MQ=255)
                                                            gCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAg                                                                                      <  1:1905403/149‑1 (MQ=255)
                                                                tAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGAc                                                                                  <  2:2758754/149‑1 (MQ=255)
                                                                 aGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACt                                                                                 <  1:4631980/149‑1 (MQ=255)
                                                                 aGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACt                                                                                 >  1:1580847/1‑149 (MQ=255)
                                                                  ggCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACtt                                                                                >  1:4183609/1‑149 (MQ=255)
                                                                     aCTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCAGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTAAc                                                                             >  1:2465925/1‑149 (MQ=255)
                                                                        ggCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCAcccc                                                                          <  2:4704091/149‑1 (MQ=255)
                                                                          cACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCaa                                                                        <  2:1407145/149‑1 (MQ=255)
                                                                            cGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGGTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCGGACGTGCCTTAGTAGGCAGGGGCGAAAGGGCCGTCACCCCAATa                                                                      >  1:1318368/1‑149 (MQ=255)
                                                                              gTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATaca                                                                    >  1:2690395/1‑149 (MQ=255)
                                                                                     tAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACgg                                                             <  2:3031918/149‑1 (MQ=255)
                                                                                       aTGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCg                                                           >  2:2712121/1‑149 (MQ=255)
                                                                                         ggCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGtt                                                         >  2:1003154/1‑149 (MQ=255)
                                                                                         ggCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGtt                                                         <  2:2311018/149‑1 (MQ=255)
                                                                                          gCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGttt                                                        >  2:3960148/1‑149 (MQ=255)
                                                                                              cTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAg                                                    >  2:2124700/1‑149 (MQ=255)
                                                                                                tCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGt                                                  >  1:1565573/1‑149 (MQ=255)
                                                                                                  tGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGtat                                                <  1:3562843/149‑1 (MQ=255)
                                                                                                   gCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGtata                                               <  1:354764/149‑1 (MQ=255)
                                                                                                    cACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATAc                                              <  1:1354367/149‑1 (MQ=255)
                                                                                                        gggTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGc                                          <  1:967924/149‑1 (MQ=255)
                                                                                                           tATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTaa                                       >  2:4229886/1‑149 (MQ=255)
                                                                                                             ttCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGTGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTaaaa                                     >  2:1109621/1‑149 (MQ=255)
                                                                                                             ttCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTaaaa                                     >  1:3304781/1‑149 (MQ=255)
                                                                                                             ttCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTaaaa                                     >  2:1320617/1‑149 (MQ=255)
                                                                                                                ttgttgCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAatt                                  <  1:2369429/149‑1 (MQ=255)
                                                                                                                 tgttgCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAatta                                 >  1:3112804/1‑149 (MQ=255)
                                                                                                                  gttgCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAattat                                >  2:573176/1‑149 (MQ=255)
                                                                                                                  gttgCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAattat                                >  2:1621451/1‑149 (MQ=255)
                                                                                                                  ggtgCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAattat                                <  2:62932/147‑1 (MQ=255)
                                                                                                                    tgCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTc                              <  2:368632/149‑1 (MQ=255)
                                                                                                                    tgCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTc                              <  1:4304951/149‑1 (MQ=255)
                                                                                                                     gCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCa                             <  1:2583252/149‑1 (MQ=255)
                                                                                                                      cTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAg                            <  1:2719648/149‑1 (MQ=255)
                                                                                                                      cTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACATGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAg                            <  2:4539266/149‑1 (MQ=255)
                                                                                                                        gggTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCg                          >  2:4134149/1‑149 (MQ=255)
                                                                                                                        gggTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCg                          >  1:718047/1‑149 (MQ=255)
                                                                                                                            tGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGtt                      <  2:355100/149‑1 (MQ=255)
                                                                                                                            tGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGtt                      <  2:358064/149‑1 (MQ=255)
                                                                                                                            tGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGtt                      >  2:2001320/1‑149 (MQ=255)
                                                                                                                              gCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTg                    <  2:608227/149‑1 (MQ=255)
                                                                                                                               cGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTgg                   <  1:1621655/149‑1 (MQ=255)
                                                                                                                               cGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTgg                   >  1:808880/1‑149 (MQ=255)
                                                                                                                                 gtgtTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGaa                 >  2:2421030/1‑149 (MQ=255)
                                                                                                                                 gtgtTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGaa                 >  2:3080604/1‑149 (MQ=255)
                                                                                                                                 gtgtTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGaa                 >  2:1882968/1‑149 (MQ=255)
                                                                                                                                  tgtTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGaaa                >  2:691262/1‑149 (MQ=255)
                                                                                                                                   gtTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAAc               >  2:185750/1‑149 (MQ=255)
                                                                                                                                   gtTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAAc               <  2:2000413/149‑1 (MQ=255)
                                                                                                                                   gtTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAAc               <  1:3013814/149‑1 (MQ=255)
                                                                                                                                       aTTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAg           >  2:905492/1‑149 (MQ=255)
                                                                                                                                        ttttGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGa          <  2:1062457/149‑1 (MQ=255)
                                                                                                                                          ttGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATg        <  1:2792066/149‑1 (MQ=255)
                                                                                                                                           tgatgaGCTGGAGATCTCGATTACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGTGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATgg       <  1:587748/149‑1 (MQ=255)
                                                                                                                                            gatgaGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGc      >  2:3132863/1‑149 (MQ=255)
                                                                                                                                            gatgaGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGc      <  1:3859523/149‑1 (MQ=255)
                                                                                                                                                aGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCaaaa  <  1:2517709/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAA  >  CP009273/371944‑372236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTTA  >  CP009273/371944‑372240
                                                                                                                                                   |                                                                                                                                                     
GCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGG                                                                                                                                                     <  SRR19973071.2522535/150‑1 (MQ=60)
GCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGG                                                                                                                                                     <  SRR19973071.992420/150‑1 (MQ=60)
     GGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATC                                                                                                                                                >  SRR19973071.654692/1‑150 (MQ=60)
     GGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATC                                                                                                                                                >  SRR19973071.3054384/1‑150 (MQ=60)
        GGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACG                                                                                                                                             >  SRR19973071.669890/1‑150 (MQ=60)
           AGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAAT                                                                                                                                          <  SRR19973071.3041109/150‑1 (MQ=60)
             GGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATAC                                                                                                                                        <  SRR19973071.1247492/150‑1 (MQ=60)
             GGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATAC                                                                                                                                        <  SRR19973071.3034715/150‑1 (MQ=60)
               CGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAG                                                                                                                                      >  SRR19973071.1760966/1‑150 (MQ=60)
               CGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAG                                                                                                                                      >  SRR19973071.1134662/1‑150 (MQ=60)
                GTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGC                                                                                                                                     <  SRR19973071.935178/150‑1 (MQ=60)
                 TCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCC                                                                                                                                    <  SRR19973071.2300276/150‑1 (MQ=60)
                  CTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCG                                                                                                                                   <  SRR19973071.1201691/150‑1 (MQ=60)
                      GGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCG                                                                                                                               <  SRR19973071.4447134/150‑1 (MQ=60)
                      GGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCG                                                                                                                               <  SRR19973071.4447184/150‑1 (MQ=60)
                       GGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGC                                                                                                                              >  SRR19973071.2617305/1‑150 (MQ=60)
                       GGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGC                                                                                                                              >  SRR19973071.4460234/1‑150 (MQ=60)
                           ATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGAT                                                                                                                          >  SRR19973071.4028086/1‑150 (MQ=60)
                           ATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGAT                                                                                                                          >  SRR19973071.4028088/1‑150 (MQ=60)
                            TGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATG                                                                                                                         <  SRR19973071.1342521/150‑1 (MQ=60)
                              AGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCC                                                                                                                       <  SRR19973071.1347513/150‑1 (MQ=60)
                              AGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCC                                                                                                                       <  SRR19973071.3952945/150‑1 (MQ=60)
                               GCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCT                                                                                                                      <  SRR19973071.513791/150‑1 (MQ=60)
                               GCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCT                                                                                                                      <  SRR19973071.2305763/150‑1 (MQ=60)
                                CGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATTAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTG                                                                                                                     >  SRR19973071.1243147/1‑150 (MQ=60)
                                  GTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGAC                                                                                                                   >  SRR19973071.828844/1‑150 (MQ=60)
                                  GTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGAC                                                                                                                   >  SRR19973071.889894/1‑150 (MQ=60)
                                   TCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACG                                                                                                                  >  SRR19973071.2740000/1‑150 (MQ=60)
                                    CCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGT                                                                                                                 >  SRR19973071.3957695/1‑150 (MQ=60)
                                     CGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTG                                                                                                                >  SRR19973071.2521788/1‑150 (MQ=60)
                                     CGTGACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTG                                                                                                                <  SRR19973071.1284168/150‑1 (MQ=60)
                                      GTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGC                                                                                                               <  SRR19973071.4738789/150‑1 (MQ=60)
                                      GTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGC                                                                                                               >  SRR19973071.2109044/1‑150 (MQ=60)
                                       TTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCC                                                                                                              >  SRR19973071.1611895/1‑150 (MQ=60)
                                       TTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCC                                                                                                              >  SRR19973071.1611929/1‑150 (MQ=60)
                                        TACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCT                                                                                                             >  SRR19973071.1345247/1‑150 (MQ=60)
                                          CTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTA                                                                                                           >  SRR19973071.2172265/1‑150 (MQ=60)
                                          CTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTA                                                                                                           >  SRR19973071.2044078/1‑150 (MQ=60)
                                              CCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAG                                                                                                       <  SRR19973071.1903807/150‑1 (MQ=60)
                                                   AAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGG                                                                                                  >  SRR19973071.3930761/1‑150 (MQ=60)
                                                   AAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGG                                                                                                  >  SRR19973071.3931458/1‑150 (MQ=60)
                                                    AAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGG                                                                                                 <  SRR19973071.4634388/150‑1 (MQ=60)
                                                     AATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGG                                                                                                <  SRR19973071.262086/150‑1 (MQ=60)
                                                       TGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCG                                                                                              <  SRR19973071.3704592/150‑1 (MQ=60)
                                                         CTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAA                                                                                            <  SRR19973071.337787/150‑1 (MQ=60)
                                                         CTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAA                                                                                            <  SRR19973071.337802/150‑1 (MQ=60)
                                                          TGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAA                                                                                           >  SRR19973071.1181544/1‑150 (MQ=60)
                                                           GGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAG                                                                                          <  SRR19973071.935606/150‑1 (MQ=60)
                                                           GGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAG                                                                                          <  SRR19973071.1901938/150‑1 (MQ=60)
                                                           GGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAG                                                                                          <  SRR19973071.3501352/150‑1 (MQ=60)
                                                           GGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAG                                                                                          <  SRR19973071.3501203/150‑1 (MQ=60)
                                                           GGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAG                                                                                          <  SRR19973071.2857087/150‑1 (MQ=60)
                                                            GCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGG                                                                                         <  SRR19973071.1912395/150‑1 (MQ=60)
                                                             CATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGG                                                                                        >  SRR19973071.1927257/1‑150 (MQ=60)
                                                              ATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGA                                                                                       <  SRR19973071.2566558/150‑1 (MQ=60)
                                                                TAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACT                                                                                     >  SRR19973071.1586451/1‑150 (MQ=60)
                                                                TAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACT                                                                                     >  SRR19973071.1586448/1‑150 (MQ=60)
                                                                 AGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTT                                                                                    <  SRR19973071.4648463/150‑1 (MQ=60)
                                                                 AGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTT                                                                                    <  SRR19973071.4648481/150‑1 (MQ=60)
                                                                 AGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCGTATTAGGCAGGGGCGAAAGGGACTT                                                                                    >  SRR19973071.4198498/1‑150 (MQ=60)
                                                                  GGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTC                                                                                   >  SRR19973071.621728/1‑150 (MQ=60)
                                                                    CACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCAGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTAAC                                                                                 >  SRR19973071.2474891/1‑150 (MQ=60)
                                                                       TGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCC                                                                              >  SRR19973071.2700318/1‑150 (MQ=60)
                                                                          CACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAAT                                                                           <  SRR19973071.1683622/150‑1 (MQ=60)
                                                                           ACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGGTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCGGACGTGCCTTAGTAGGCAGGGGCGAAAGGGCCGTCACCCCAATA                                                                          >  SRR19973071.1323200/1‑150 (MQ=60)
                                                                             GGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACA                                                                        >  SRR19973071.2700008/1‑150 (MQ=60)
                                                                             GGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACA                                                                        >  SRR19973071.3363185/1‑150 (MQ=60)
                                                                             GGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACA                                                                        >  SRR19973071.2943000/1‑150 (MQ=60)
                                                                              GTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGGTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACAC                                                                       <  SRR19973071.965117/150‑1 (MQ=60)
                                                                                CGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCT                                                                     <  SRR19973071.4289944/150‑1 (MQ=60)
                                                                                         GGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTT                                                            <  SRR19973071.2194314/150‑1 (MQ=60)
                                                                                           CCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTAC                                                          >  SRR19973071.679140/1‑150 (MQ=60)
                                                                                             GCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAG                                                        >  SRR19973071.251112/1‑150 (MQ=60)
                                                                                             GCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAG                                                        >  SRR19973071.1822060/1‑150 (MQ=60)
                                                                                               TTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGT                                                      >  SRR19973071.1571192/1‑150 (MQ=60)
                                                                                               TTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGT                                                      >  SRR19973071.1571134/1‑150 (MQ=60)
                                                                                                  TGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATA                                                   <  SRR19973071.442061/150‑1 (MQ=60)
                                                                                                  TGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATA                                                   <  SRR19973071.3574853/150‑1 (MQ=60)
                                                                                                  TGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATA                                                   <  SRR19973071.3575254/150‑1 (MQ=60)
                                                                                                   GCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATAC                                                  <  SRR19973071.355919/150‑1 (MQ=60)
                                                                                                    CACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACT                                                 <  SRR19973071.1359343/150‑1 (MQ=60)
                                                                                                    CACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACT                                                 <  SRR19973071.1359348/150‑1 (MQ=60)
                                                                                                    CACCGGGTATTCTTGTTGCTGGGTTGGCGGTGCTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAACACACCTACGGCGTTTACAGGTATACT                                                 <  SRR19973071.1359402/150‑1 (MQ=60)
                                                                                                        GGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTAAAGGTATACTCGCT                                             >  SRR19973071.1227592/1‑150 (MQ=60)
                                                                                                        GGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCT                                             <  SRR19973071.971638/150‑1 (MQ=60)
                                                                                                        GGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCT                                             <  SRR19973071.971443/150‑1 (MQ=60)
                                                                                                            ATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAA                                         >  SRR19973071.2153006/1‑150 (MQ=60)
                                                                                                            ATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAA                                         >  SRR19973071.3316497/1‑150 (MQ=60)
                                                                                                             TTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTAGCTAAAAA                                        >  SRR19973071.4435071/1‑150 (MQ=60)
                                                                                                                TTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTA                                     >  SRR19973071.3123761/1‑150 (MQ=60)
                                                                                                                TTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTA                                     >  SRR19973071.3124169/1‑150 (MQ=60)
                                                                                                                TTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTA                                     <  SRR19973071.2377995/150‑1 (MQ=60)
                                                                                                                   TTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTC                                  <  SRR19973071.2210945/150‑1 (MQ=60)
                                                                                                                    TGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCA                                 <  SRR19973071.4320177/150‑1 (MQ=60)
                                                                                                                     GCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAG                                <  SRR19973071.2592620/150‑1 (MQ=60)
                                                                                                                      CTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGC                               <  SRR19973071.2729324/150‑1 (MQ=60)
                                                                                                                      CTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGC                               <  SRR19973071.2729321/150‑1 (MQ=60)
                                                                                                                       TGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCG                              >  SRR19973071.720550/1‑150 (MQ=60)
                                                                                                                       TGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCG                              >  SRR19973071.2398201/1‑150 (MQ=60)
                                                                                                                          GTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGT                           <  SRR19973071.1635981/150‑1 (MQ=60)
                                                                                                                              GCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGG                       >  SRR19973071.811595/1‑150 (MQ=60)
                                                                                                                               CGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGA                      <  SRR19973071.1627455/150‑1 (MQ=60)
                                                                                                                               CGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGA                      <  SRR19973071.1627250/150‑1 (MQ=60)
                                                                                                                                 GTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTCAAAATTATTCAGCGGGTTTGGAAA                    >  SRR19973071.811077/1‑150 (MQ=60)
                                                                                                                                 GTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAA                    >  SRR19973071.811229/1‑150 (MQ=60)
                                                                                                                                  TGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAAC                   >  SRR19973071.3488226/1‑150 (MQ=60)
                                                                                                                                   GTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACA                  >  SRR19973071.898979/1‑150 (MQ=60)
                                                                                                                                   GTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACA                  <  SRR19973071.261015/150‑1 (MQ=60)
                                                                                                                                   GTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACA                  <  SRR19973071.3024567/150‑1 (MQ=60)
                                                                                                                                      TATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAG               <  SRR19973071.3659247/150‑1 (MQ=60)
                                                                                                                                          TTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGG           <  SRR19973071.2801857/150‑1 (MQ=60)
                                                                                                                                           TGATGAGCTGGAGATCTCGATTACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGTGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGC          <  SRR19973071.589936/150‑1 (MQ=60)
                                                                                                                                           TGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGC          <  SRR19973071.589565/150‑1 (MQ=60)
                                                                                                                                            GATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCA         <  SRR19973071.3873191/150‑1 (MQ=60)
                                                                                                                                             ATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAA        >  SRR19973071.3272093/1‑150 (MQ=60)
                                                                                                                                                AGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAAC     <  SRR19973071.2501390/150‑1 (MQ=60)
                                                                                                                                                AGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAAC     <  SRR19973071.2526874/150‑1 (MQ=60)
                                                                                                                                                AGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAAC     <  SRR19973071.2526886/150‑1 (MQ=60)
                                                                                                                                                 GCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACT    <  SRR19973071.644484/150‑1 (MQ=60)
                                                                                                                                                  CAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTT   >  SRR19973071.3111561/1‑150 (MQ=60)
                                                                                                                                                   AGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTTA  >  SRR19973071.3857323/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
GCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTTA  >  CP009273/371944‑372240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑