Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 372,091 C→A 100% R393R (CGG→AGG)  mhpT → 3‑hydroxyphenylpropionic transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273372,0910CA98.8% 791.3 / 0.2 258R393R (CGG→AGG) mhpT3‑hydroxyphenylpropionic transporter
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (119/134);  total (121/137)
Fisher's exact test for biased strand distribution p-value = 6.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 259 total aligned reads displayed.

CAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAA  >  CP009273/371942‑372236
                                                                                                                                                     |                                                                                                                                                 
cAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGcc                                                                                                                                                   >  1:4464329/1‑150 (MQ=255)
  gCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAgg                                                                                                                                                 <  2:699114/150‑1 (MQ=255)
    cGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGgag                                                                                                                                               <  2:2563509/150‑1 (MQ=255)
     gTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGgaga                                                                                                                                              <  2:1093094/150‑1 (MQ=255)
      tGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGAt                                                                                                                                             <  2:247503/150‑1 (MQ=255)
      tGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGAt                                                                                                                                             <  1:2248016/150‑1 (MQ=255)
       ggCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATc                                                                                                                                            <  1:1256542/150‑1 (MQ=255)
         cGGTAGGGCGTCTGGGGTCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCTGGAGATCAc                                                                                                                                          <  1:2565051/150‑1 (MQ=255)
         cGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCAc                                                                                                                                          <  2:1052597/150‑1 (MQ=255)
          tgTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAATATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACg                                                                                                                                         <  2:1029714/149‑1 (MQ=255)
          gggaGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACg                                                                                                                                         <  2:4177293/147‑1 (MQ=255)
            tAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGaa                                                                                                                                       >  1:2364234/1‑150 (MQ=255)
             aGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAAt                                                                                                                                      >  1:3612717/1‑150 (MQ=255)
                gCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACa                                                                                                                                   <  2:4426417/150‑1 (MQ=255)
                gCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGG‑AAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAg                                                                                                                                  >  2:1341493/1‑150 (MQ=255)
                 cGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAg                                                                                                                                  <  2:510488/150‑1 (MQ=255)
                 cGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAg                                                                                                                                  <  2:3187666/150‑1 (MQ=255)
                     tGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGt                                                                                                                              <  1:2719070/150‑1 (MQ=255)
                      gggggCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTg                                                                                                                             >  1:1382911/1‑150 (MQ=255)
                           cTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCg                                                                                                                        >  2:465347/1‑150 (MQ=255)
                             aTGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGAt                                                                                                                      <  1:2348531/150‑1 (MQ=255)
                             aTGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGAt                                                                                                                      >  2:3749770/1‑150 (MQ=255)
                              tGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATg                                                                                                                     <  1:4569042/150‑1 (MQ=255)
                                 gCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCt                                                                                                                  <  1:596962/150‑1 (MQ=255)
                                 gCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCt                                                                                                                  >  2:2971476/1‑150 (MQ=255)
                                  cGGTCCGTGACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTg                                                                                                                 <  1:954771/150‑1 (MQ=255)
                                   ggTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGa                                                                                                                >  1:291624/1‑150 (MQ=255)
                                      ccGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGt                                                                                                             >  2:1967002/1‑150 (MQ=255)
                                       cGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTg                                                                                                            >  2:2336354/1‑150 (MQ=255)
                                          tACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCt                                                                                                         <  1:1487804/150‑1 (MQ=255)
                                           aCTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCtt                                                                                                        >  1:5037973/1‑150 (MQ=255)
                                              ggCCGGGAAAATGCTAGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCttatt                                                                                                     <  1:4174149/150‑1 (MQ=255)
                                                ccGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAg                                                                                                   <  1:90696/150‑1 (MQ=255)
                                                        aTGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGc                                                                                           <  2:2403659/150‑1 (MQ=255)
                                                        aTGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGc                                                                                           >  1:2807086/1‑150 (MQ=255)
                                                           cTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGaa                                                                                        >  2:5325952/1‑150 (MQ=255)
                                                           cTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGaa                                                                                        >  2:1480176/1‑150 (MQ=255)
                                                           cTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGaa                                                                                        <  2:4588994/150‑1 (MQ=255)
                                                            tGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGaaa                                                                                       >  1:43331/1‑150 (MQ=255)
                                                                aTTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGa                                                                                   <  2:3684777/150‑1 (MQ=255)
                                                                aTTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGa                                                                                   >  2:4259624/1‑150 (MQ=255)
                                                                 ttAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGAc                                                                                  <  1:3285632/150‑1 (MQ=255)
                                                                  tAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACt                                                                                 <  2:5246362/150‑1 (MQ=255)
                                                                  tAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACt                                                                                 <  1:743855/150‑1 (MQ=255)
                                                                  tAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACt                                                                                 <  1:744149/150‑1 (MQ=255)
                                                                   aGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACtt                                                                                >  2:4722189/1‑150 (MQ=255)
                                                                    ggCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTc                                                                               <  2:3584790/150‑1 (MQ=255)
                                                                    ggCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTc                                                                               <  2:2235716/150‑1 (MQ=255)
                                                                    ggCACAGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCGGGGTTGGCGGTGTTTATTTTGATGAGCAGGATATCACGAATACATCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTc                                                                               >  1:104652/1‑150 (MQ=255)
                                                                      cACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCAc                                                                             <  1:1642519/150‑1 (MQ=255)
                                                                      cACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCAc                                                                             >  1:3295560/1‑150 (MQ=255)
                                                                        cTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCAccc                                                                           <  2:3236745/150‑1 (MQ=255)
                                                                         tGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCAcccc                                                                          <  2:367406/150‑1 (MQ=255)
                                                                              cGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATac                                                                     <  2:4193293/150‑1 (MQ=255)
                                                                               ggTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATaca                                                                    >  1:322527/1‑150 (MQ=255)
                                                                               ggTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATaca                                                                    >  1:2929221/1‑150 (MQ=255)
                                                                                gTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATacac                                                                   <  2:5298305/150‑1 (MQ=255)
                                                                                gTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATacac                                                                   <  1:4284924/150‑1 (MQ=255)
                                                                                  cGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGTCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCt                                                                 >  2:4077547/1‑150 (MQ=255)
                                                                                    gCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTAc                                                               <  2:3447111/150‑1 (MQ=255)
                                                                                    gCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTAc                                                               <  2:5017958/150‑1 (MQ=255)
                                                                                    gCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTAc                                                               <  1:3458853/150‑1 (MQ=255)
                                                                                      gTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACgg                                                             <  1:674572/150‑1 (MQ=255)
                                                                                        aaTGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCg                                                           >  1:825879/1‑150 (MQ=255)
                                                                                          tGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGtt                                                         <  1:523503/150‑1 (MQ=255)
                                                                                          tGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGtt                                                         >  2:1529693/1‑150 (MQ=255)
                                                                                           ggCCGCTTCTGCATCGGGTATTATTGTTGCTGGGTTGGCGTTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGGCTATTCATTTCGTGCCTTTTTAGGCAGGGTCGAAAGGGACTTCACCCCAATACACCTACGTCGttt                                                        <  2:5169153/150‑1 (MQ=255)
                                                                                            gCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTa                                                       <  2:122525/150‑1 (MQ=255)
                                                                                             ccGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTAc                                                      <  2:4890922/150‑1 (MQ=255)
                                                                                              cGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACa                                                     <  1:1967002/150‑1 (MQ=255)
                                                                                                   cTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGtat                                                <  1:2298627/150‑1 (MQ=255)
                                                                                                   cTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGtat                                                <  1:856188/150‑1 (MQ=255)
                                                                                                    tGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGtata                                               <  1:2009043/150‑1 (MQ=255)
                                                                                                    tGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGtata                                               <  2:2598331/150‑1 (MQ=255)
                                                                                                      cACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCTGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACt                                             <  2:283914/150‑1 (MQ=255)
                                                                                                      cACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACt                                             <  2:273853/150‑1 (MQ=255)
                                                                                                       agggggTATTGTTGTTGCTGGGTTGGAGGTGTTTATTTTGATGAGCAGGAGCTCTCGAATTCAGCCGTGCGCCGATGCCTGACTTGCATTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGAGTTTACAGGTATACTc                                            <  2:3088832/147‑1 (MQ=255)
                                                                                                          gggTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCt                                         >  2:3963709/1‑150 (MQ=255)
                                                                                                          gggTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCt                                         >  2:3506732/1‑150 (MQ=255)
                                                                                                           ggTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTa                                        <  2:623931/150‑1 (MQ=255)
                                                                                                            gTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTaa                                       >  2:2629059/1‑150 (MQ=255)
                                                                                                                 cTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAatt                                  <  1:2067781/150‑1 (MQ=255)
                                                                                                                     ttgCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTc                              <  2:1872559/150‑1 (MQ=255)
                                                                                                                      tgCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCa                             <  1:4644950/150‑1 (MQ=255)
                                                                                                                        cTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGc                           >  2:4790446/1‑150 (MQ=255)
                                                                                                                         tGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCg                          <  1:3355933/150‑1 (MQ=255)
                                                                                                                          gggTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCgg                         >  1:6803/1‑150 (MQ=255)
                                                                                                                          gggTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCgg                         >  2:207403/1‑150 (MQ=255)
                                                                                                                           ggTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTCTTCAGCggg                        >  2:2666043/1‑150 (MQ=255)
                                                                                                                            gTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGt                       >  1:5085938/1‑150 (MQ=255)
                                                                                                                            gTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTAAGCGGGt                       >  1:3127334/1‑150 (MQ=255)
                                                                                                                             ttGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGtt                      >  1:5292452/1‑150 (MQ=255)
                                                                                                                               ggCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTg                    >  2:4437188/1‑150 (MQ=255)
                                                                                                                               ggCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTg                    <  2:1536200/150‑1 (MQ=255)
                                                                                                                                 cGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGa                  <  1:1260911/150‑1 (MQ=255)
                                                                                                                                         aTTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAATGa          >  2:6843/1‑150 (MQ=255)
                                                                                                                                         aTTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGa          >  1:1102663/1‑150 (MQ=255)
                                                                                                                                              gatgaGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCa     <  2:4584022/150‑1 (MQ=255)
                                                                                                                                               atgaGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCaa    >  1:3962953/1‑150 (MQ=255)
                                                                                                                                                tgaGCAGGAGATCACGAATACAGTCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCaaa   <  1:4077547/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
CAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAA  >  CP009273/371942‑372236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTT  >  CP009273/371942‑372239
                                                                                                                                                     |                                                                                                                                                    
CAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCC                                                                                                                                                      >  SRR19973062.4487129/1‑150 (MQ=60)
      TGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCTCGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGAT                                                                                                                                                <  SRR19973062.3807947/150‑1 (MQ=60)
      TGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGAT                                                                                                                                                <  SRR19973062.2259233/150‑1 (MQ=60)
      TGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGAT                                                                                                                                                <  SRR19973062.829949/150‑1 (MQ=60)
      TGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCAACGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGAT                                                                                                                                                <  SRR19973062.2349428/150‑1 (MQ=60)
       GGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATC                                                                                                                                               <  SRR19973062.1262341/150‑1 (MQ=60)
         CGGTAGGGCGTCTGGGGTCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCTGGAGATCAC                                                                                                                                             <  SRR19973062.2578698/150‑1 (MQ=60)
         CGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGGTGGCGGTGTTTATTTTGATGAGCAGGAGATCAC                                                                                                                                             <  SRR19973062.1102798/150‑1 (MQ=60)
          GGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACG                                                                                                                                            >  SRR19973062.5289644/1‑150 (MQ=60)
           GTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCATGAGATCACGA                                                                                                                                           >  SRR19973062.4343323/1‑150 (MQ=60)
            TAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAA                                                                                                                                          >  SRR19973062.2376876/1‑150 (MQ=60)
            TAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGGGTTTATTTTGATGAGCCGGAGATCACGAA                                                                                                                                          >  SRR19973062.2376861/1‑150 (MQ=60)
             AGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAAT                                                                                                                                         >  SRR19973062.656349/1‑150 (MQ=60)
             AGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAAT                                                                                                                                         >  SRR19973062.3631131/1‑150 (MQ=60)
              GGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATA                                                                                                                                        >  SRR19973062.4642694/1‑150 (MQ=60)
                     TGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGT                                                                                                                                 >  SRR19973062.1262397/1‑150 (MQ=60)
                     TGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGT                                                                                                                                 <  SRR19973062.2733380/150‑1 (MQ=60)
                      GGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTG                                                                                                                                >  SRR19973062.1389408/1‑150 (MQ=60)
                      GGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTG                                                                                                                                >  SRR19973062.3014172/1‑150 (MQ=60)
                        GGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCG                                                                                                                              <  SRR19973062.1176664/150‑1 (MQ=60)
                         GGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGC                                                                                                                             >  SRR19973062.5341067/1‑150 (MQ=60)
                             ATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGAT                                                                                                                         <  SRR19973062.2361034/150‑1 (MQ=60)
                              TGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATG                                                                                                                        <  SRR19973062.4592909/150‑1 (MQ=60)
                              TGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATG                                                                                                                        <  SRR19973062.2449293/150‑1 (MQ=60)
                                 GCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCT                                                                                                                     <  SRR19973062.3291005/150‑1 (MQ=60)
                                 GCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCT                                                                                                                     <  SRR19973062.3350858/150‑1 (MQ=60)
                                 GCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCT                                                                                                                     <  SRR19973062.599759/150‑1 (MQ=60)
                                 GCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCT                                                                                                                     <  SRR19973062.890385/150‑1 (MQ=60)
                                  CGGTCCGTGACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTG                                                                                                                    <  SRR19973062.959142/150‑1 (MQ=60)
                                   GGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGA                                                                                                                   >  SRR19973062.293042/1‑150 (MQ=60)
                                   GGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGA                                                                                                                   >  SRR19973062.293099/1‑150 (MQ=60)
                                    GTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGAC                                                                                                                  <  SRR19973062.4381591/150‑1 (MQ=60)
                                      CCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGT                                                                                                                <  SRR19973062.2081296/150‑1 (MQ=60)
                                         TTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCC                                                                                                             <  SRR19973062.3060609/150‑1 (MQ=60)
                                         TTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCC                                                                                                             <  SRR19973062.74901/150‑1 (MQ=60)
                                          TACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCT                                                                                                            <  SRR19973062.1495238/150‑1 (MQ=60)
                                          TACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCT                                                                                                            <  SRR19973062.1495102/150‑1 (MQ=60)
                                           ACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTT                                                                                                           >  SRR19973062.5064670/1‑150 (MQ=60)
                                              GGCCGGGAAAATGCTAGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATT                                                                                                        <  SRR19973062.4195811/150‑1 (MQ=60)
                                                CCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAG                                                                                                      <  SRR19973062.91297/150‑1 (MQ=60)
                                                 CGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGG                                                                                                     >  SRR19973062.1169958/1‑150 (MQ=60)
                                                     AAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGG                                                                                                 >  SRR19973062.2762798/1‑150 (MQ=60)
                                                      AAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGG                                                                                                >  SRR19973062.695256/1‑150 (MQ=60)
                                                      AAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGG                                                                                                >  SRR19973062.695438/1‑150 (MQ=60)
                                                      AAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGG                                                                                                >  SRR19973062.3667483/1‑150 (MQ=60)
                                                      AAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGG                                                                                                >  SRR19973062.592011/1‑150 (MQ=60)
                                                       AATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGG                                                                                               >  SRR19973062.4704338/1‑150 (MQ=60)
                                                        ATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGC                                                                                              >  SRR19973062.2821936/1‑150 (MQ=60)
                                                         TGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGGATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCG                                                                                             >  SRR19973062.1300435/1‑150 (MQ=60)
                                                            TGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAA                                                                                          >  SRR19973062.43641/1‑150 (MQ=60)
                                                            TGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAA                                                                                          >  SRR19973062.1963911/1‑150 (MQ=60)
                                                             GGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAG                                                                                         >  SRR19973062.801342/1‑150 (MQ=60)
                                                             GGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAG                                                                                         >  SRR19973062.2259744/1‑150 (MQ=60)
                                                             GGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCGTATTAGGCAGGGGCGAAAG                                                                                         >  SRR19973062.4251227/1‑150 (MQ=60)
                                                             GGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCGTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATTAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAG                                                                                         >  SRR19973062.2259709/1‑150 (MQ=60)
                                                               CATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGG                                                                                       <  SRR19973062.3206335/150‑1 (MQ=60)
                                                                 TTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGAC                                                                                     <  SRR19973062.3302649/150‑1 (MQ=60)
                                                                  TAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACT                                                                                    >  SRR19973062.3401597/1‑150 (MQ=60)
                                                                  TAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACT                                                                                    <  SRR19973062.747230/150‑1 (MQ=60)
                                                                  TAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACT                                                                                    <  SRR19973062.747525/150‑1 (MQ=60)
                                                                    GGCACAGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCGGGGTTGGCGGTGTTTATTTTGATGAGCAGGATATCACGAATACATCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTC                                                                                  >  SRR19973062.105321/1‑150 (MQ=60)
                                                                      CACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCAC                                                                                >  SRR19973062.3312616/1‑150 (MQ=60)
                                                                      CACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCAC                                                                                <  SRR19973062.1650609/150‑1 (MQ=60)
                                                                      CACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCGTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTGATTAGGCAGGGGCGAAAGGGACTTCAC                                                                                >  SRR19973062.3312615/1‑150 (MQ=60)
                                                                            CACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAAT                                                                          <  SRR19973062.4545984/150‑1 (MQ=60)
                                                                            CACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAAT                                                                          <  SRR19973062.4083346/150‑1 (MQ=60)
                                                                               GGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACA                                                                       >  SRR19973062.2944644/1‑150 (MQ=60)
                                                                               GGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACA                                                                       >  SRR19973062.324026/1‑150 (MQ=60)
                                                                                GTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACAC                                                                      <  SRR19973062.4307075/150‑1 (MQ=60)
                                                                                 TCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACC                                                                     >  SRR19973062.5087725/1‑150 (MQ=60)
                                                                                   GGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGATATAAAGAATAAAGCCGTTCGCCTATTCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTAACCCCAATACACCTA                                                                   >  SRR19973062.1284373/1‑150 (MQ=60)
                                                                                    GCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTAC                                                                  <  SRR19973062.5164696/150‑1 (MQ=60)
                                                                                    GCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTAC                                                                  <  SRR19973062.3476481/150‑1 (MQ=60)
                                                                                      GTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGG                                                                <  SRR19973062.677703/150‑1 (MQ=60)
                                                                                        AATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCG                                                              >  SRR19973062.829507/1‑150 (MQ=60)
                                                                                          TGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTT                                                            <  SRR19973062.525976/150‑1 (MQ=60)
                                                                                              CGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACA                                                        <  SRR19973062.1977113/150‑1 (MQ=60)
                                                                                                   CTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTAT                                                   <  SRR19973062.859879/150‑1 (MQ=60)
                                                                                                   CTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTAT                                                   <  SRR19973062.2033495/150‑1 (MQ=60)
                                                                                                   CTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTAT                                                   <  SRR19973062.2310595/150‑1 (MQ=60)
                                                                                                   CTGCACCGGGTATTCGTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTAT                                                   <  SRR19973062.1479284/150‑1 (MQ=60)
                                                                                                    TGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATA                                                  <  SRR19973062.2019374/150‑1 (MQ=60)
                                                                                                    TGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATA                                                  <  SRR19973062.2019408/150‑1 (MQ=60)
                                                                                                       ACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTC                                               <  SRR19973062.2013958/150‑1 (MQ=60)
                                                                                                              ATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAA                                        >  SRR19973062.3547915/1‑150 (MQ=60)
                                                                                                                 CTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATT                                     >  SRR19973062.2800629/1‑150 (MQ=60)
                                                                                                                 CTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATT                                     <  SRR19973062.2078366/150‑1 (MQ=60)
                                                                                                                 CTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATT                                     <  SRR19973062.956834/150‑1 (MQ=60)
                                                                                                                 CTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATT                                     >  SRR19973062.765149/1‑150 (MQ=60)
                                                                                                                   TGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTAT                                   <  SRR19973062.1774245/150‑1 (MQ=60)
                                                                                                                    GTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATT                                  >  SRR19973062.933250/1‑150 (MQ=60)
                                                                                                                     TTGCTGGGTTGGCTGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTC                                 <  SRR19973062.887997/150‑1 (MQ=60)
                                                                                                                     TTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTC                                 <  SRR19973062.429016/150‑1 (MQ=60)
                                                                                                                      TGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCA                                <  SRR19973062.4669253/150‑1 (MQ=60)
                                                                                                                         TGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCG                             <  SRR19973062.3373219/150‑1 (MQ=60)
                                                                                                                         TGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCG                             <  SRR19973062.3373214/150‑1 (MQ=60)
                                                                                                                         TGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCG                             <  SRR19973062.3373236/150‑1 (MQ=60)
                                                                                                                          GGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGG                            >  SRR19973062.992881/1‑150 (MQ=60)
                                                                                                                          GGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGG                            >  SRR19973062.6856/1‑150 (MQ=60)
                                                                                                                          GGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGG                            >  SRR19973062.6840/1‑150 (MQ=60)
                                                                                                                           GGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGG                           >  SRR19973062.4190410/1‑150 (MQ=60)
                                                                                                                            GTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGT                          >  SRR19973062.5112918/1‑150 (MQ=60)
                                                                                                                            GTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGT                          >  SRR19973062.5112866/1‑150 (MQ=60)
                                                                                                                            GTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTAAGCGGGT                          >  SRR19973062.3143428/1‑150 (MQ=60)
                                                                                                                            GTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTAAGCGGGT                          >  SRR19973062.4619824/1‑150 (MQ=60)
                                                                                                                             TTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTT                         >  SRR19973062.5320262/1‑150 (MQ=60)
                                                                                                                               GGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTG                       <  SRR19973062.2588692/150‑1 (MQ=60)
                                                                                                                                 CGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGA                     <  SRR19973062.1266726/150‑1 (MQ=60)
                                                                                                                                  GGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTAgatcggaagagcacacgtctgaactcagtcactagtcgagatctcgt                    >  SRR19973062.4154982/1‑103 (MQ=60)
                                                                                                                                  GGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAA                    >  SRR19973062.2892821/1‑150 (MQ=60)
                                                                                                                                  GGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAA                    >  SRR19973062.635476/1‑150 (MQ=60)
                                                                                                                                  GGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAA                    >  SRR19973062.2548460/1‑150 (MQ=60)
                                                                                                                                  GGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAA                    >  SRR19973062.2892833/1‑150 (MQ=60)
                                                                                                                                         ATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGA             >  SRR19973062.1107847/1‑150 (MQ=60)
                                                                                                                                          TTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGAT            >  SRR19973062.4384969/1‑150 (MQ=60)
                                                                                                                                          TTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGAT            <  SRR19973062.1565651/150‑1 (MQ=60)
                                                                                                                                          TTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGAT            <  SRR19973062.5054274/150‑1 (MQ=60)
                                                                                                                                           TTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGTCGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATG           <  SRR19973062.2348767/150‑1 (MQ=60)
                                                                                                                                               ATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAA       >  SRR19973062.3983044/1‑150 (MQ=60)
                                                                                                                                                TGAGCAGGAGATCACGAATACAGTCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAA      <  SRR19973062.4098516/150‑1 (MQ=60)
                                                                                                                                                TGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAA      >  SRR19973062.3298304/1‑150 (MQ=60)
                                                                                                                                                   GCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACT   <  SRR19973062.2202454/150‑1 (MQ=60)
                                                                                                                                                    CAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTT  <  SRR19973062.2485537/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTT  >  CP009273/371942‑372239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑