Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F247 I0 R1 1496 299.8 9582018 97.8% 9371213 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,477,570 G→A 7.4% D85N (GAC→AAC)  hrpA → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,477,5700GA 7.4% 956.3 / 42.9 289D85N (GAC→AAC) hrpAputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (128/135);  new base A (11/10);  total (139/150)
Fisher's exact test for biased strand distribution p-value = 8.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01

Only 100 of 296 total aligned reads displayed.

GAAGGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGC  >  CP009273/1477422‑1477718
                                                                                                                                                     |                                                                                                                                                    
gaagGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAgg                                                                                                                                                      >  2:4012657/1‑149 (MQ=255)
 aagGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGa                                                                                                                                                     >  1:1440395/1‑149 (MQ=255)
   ggTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACa                                                                                                                                                   <  2:2618808/149‑1 (MQ=255)
      tAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATtc                                                                                                                                                <  1:4068855/149‑1 (MQ=255)
       aaaaaTCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATtct                                                                                                                                               <  1:1849325/149‑1 (MQ=255)
        aaaaTCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATtctc                                                                                                                                              <  2:3788794/149‑1 (MQ=255)
          aaTCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGa                                                                                                                                            <  1:4234337/149‑1 (MQ=255)
          aaTCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGa                                                                                                                                            >  1:2091247/1‑149 (MQ=255)
          aaTCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGa                                                                                                                                            <  1:1597530/149‑1 (MQ=255)
           aTCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGaa                                                                                                                                           <  1:3700373/149‑1 (MQ=255)
               tGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGa                                                                                                                                       >  1:1963141/1‑149 (MQ=255)
                gATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGAt                                                                                                                                      <  2:3494383/149‑1 (MQ=255)
                 aTGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGAtt                                                                                                                                     <  1:527537/149‑1 (MQ=255)
                 aTGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGAtt                                                                                                                                     <  1:2708832/149‑1 (MQ=255)
                 aTGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGAACATTCTCGAAGCGAtt                                                                                                                                     <  1:4746641/149‑1 (MQ=255)
                  tGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTc                                                                                                                                    >  1:3427216/1‑149 (MQ=255)
                  tGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTc                                                                                                                                    <  1:1360308/149‑1 (MQ=255)
                     acaacaGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGACTCGTg                                                                                                                                 >  1:1795001/1‑149 (MQ=255)
                      caacaGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGa                                                                                                                                >  2:3662508/1‑149 (MQ=255)
                      caacaGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGa                                                                                                                                >  2:3418337/1‑149 (MQ=255)
                        acaGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATc                                                                                                                              <  1:3055206/149‑1 (MQ=255)
                          aGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCAc                                                                                                                            <  2:3170101/149‑1 (MQ=255)
                           ggCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCAcc                                                                                                                           >  1:4787936/1‑149 (MQ=255)
                               aTTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAggt                                                                                                                       <  2:3142417/149‑1 (MQ=255)
                                ttttCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAggtg                                                                                                                      <  1:3217324/149‑1 (MQ=255)
                                 tttCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAggtgg                                                                                                                     >  1:400694/1‑149 (MQ=255)
                                  ttCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAggtggt                                                                                                                    <  1:4427662/149‑1 (MQ=255)
                                   tCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGgtggtg                                                                                                                   <  2:1201995/149‑1 (MQ=255)
                                    ccAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGa                                                                                                                  <  2:1174110/149‑1 (MQ=255)
                                     cAGGAGATGGCGAAATAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAt                                                                                                                 <  1:35230/149‑1 (MQ=255)
                                        gagaTGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAtcgt                                                                                                              <  2:2341716/149‑1 (MQ=255)
                                        gagaTGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAtcgt                                                                                                              <  2:979594/149‑1 (MQ=255)
                                        gagaTGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAtcgt                                                                                                              >  1:1463881/1‑149 (MQ=255)
                                         agaTGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAtcgtc                                                                                                             >  2:2785771/1‑149 (MQ=255)
                                         agaTGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAtcgtc                                                                                                             >  2:2968930/1‑149 (MQ=255)
                                            tGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGcc                                                                                                          >  1:1833384/1‑149 (MQ=255)
                                             ggCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCg                                                                                                         >  1:1117335/1‑149 (MQ=255)
                                                gAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGAGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCgggg                                                                                                      <  2:2694285/149‑1 (MQ=255)
                                                 aaaGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACACTTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGa                                                                                                     >  1:3503032/1‑149 (MQ=255)
                                                 aaaGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGa                                                                                                     >  1:4398467/1‑149 (MQ=255)
                                                  aaGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGaa                                                                                                    >  2:2467066/1‑149 (MQ=255)
                                                     agaTTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACg                                                                                                 >  2:4716359/1‑149 (MQ=255)
                                                         tGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGtt                                                                                             <  2:114849/149‑1 (MQ=255)
                                                          gACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTc                                                                                            <  2:1885574/149‑1 (MQ=255)
                                                          gACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTGACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTc                                                                                            <  2:4275503/149‑1 (MQ=255)
                                                             cAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTgg                                                                                         <  1:3824868/149‑1 (MQ=255)
                                                              aGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGt                                                                                        <  1:2341039/149‑1 (MQ=255)
                                                                gcggcAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTaa                                                                                      <  2:3356455/149‑1 (MQ=255)
                                                                  ggcAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTaaaa                                                                                    <  1:1008689/149‑1 (MQ=255)
                                                                  ggcAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTaaaa                                                                                    <  2:497065/149‑1 (MQ=255)
                                                                     aGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGa                                                                                 >  2:2836689/1‑149 (MQ=255)
                                                                     aGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGa                                                                                 >  2:4064794/1‑149 (MQ=255)
                                                                        gAAAGTCCTGCTGCGTGAAGCGGCACGACCGTAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTc                                                                              <  2:4192425/149‑1 (MQ=255)
                                                                             tCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAAGTGACCGG‑TTAGTCAGAACAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTa                                                                         >  1:326515/1‑149 (MQ=255)
                                                                               ctgctgCTTTAAGCGGCACGACCGGAAATTACTTATCATGACATTTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTTATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGTTAAAACGACTCAGTTAcc                                                                       <  2:4583730/149‑1 (MQ=255)
                                                                               ctgctgCGTGATGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGTAAACGGGTTCTGGTAAAACGACTCAGTTAcc                                                                       <  2:4591694/149‑1 (MQ=255)
                                                                               ctgatgCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTAcc                                                                       >  2:4606463/1‑149 (MQ=255)
                                                                                tgctgcGTGAAGCGGCACTACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTTATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCg                                                                      <  2:1978591/149‑1 (MQ=255)
                                                                                    gcGTTAAGCGGCACGACCGGAAATTACTTATCCTGAAAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGaaaa                                                                  <  2:2213174/149‑1 (MQ=255)
                                                                                    gcGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGaaaa                                                                  >  1:1670315/1‑149 (MQ=255)
                                                                                    gcGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGaaaa                                                                  <  2:2370073/149‑1 (MQ=255)
                                                                                      gTGACGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACTGGACATTCTCGAAGCGATTCGTTATCACCAGGTGGGGATCGTCGCCGGGGAAACGGGTTCGGGTAAAACGACTCAGTTACCGAAAATc                                                                <  2:4145833/149‑1 (MQ=255)
                                                                                         aaGCGGCACGCCCGGAAATTACTGCTCCTGACATTTTACCGG‑TTTGTCAGACAACACTGGACATTCTCGACGCGCTTCGTGATCACCTGGGGGTGATCGGCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGt                                                             <  2:3610267/149‑1 (MQ=255)
                                                                                         aaGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGt                                                             >  1:2228710/1‑149 (MQ=255)
                                                                                            cGGCACGACCGGAAATTACTTAACCGGACAATTTACCGG‑TTAGTCAGAAAAAACTGGACATTCACGACGCGTTTCGTGATCACCCGGTGGTGATCGTCGCCGGGGAAAGGGGTTCGGGTAAAACGACTCAGTTACCGAAAATCTGTATg                                                          <  1:650902/149‑1 (MQ=255)
                                                                                             ggCCCGACCGGAAATTACTTATCCGGACAAGTTCCCGG‑TGTGTCAGAACACACCGGACCGTCGGGATGCGATTCGTGCTCACCAGGGGGTGAGCGTCGCCGGGGATCCGGGTTCGGGTAAAACGACTCAGTTACCGAAACTCTGTATgg                                                         <  2:784566/149‑1 (MQ=255)
                                                                                                   aCCGTAAATTACTTATCCTTAAATTTTCCCGG‑TTAGTCATAAAAAACTGTACTTTCTCGACGCGATTCGTGATCACCCGGTGGTTATCGTCGCCGGGGAAAGGGGTTCTGTTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTgg                                                   <  1:872132/149‑1 (MQ=255)
                                                                                                         aaTTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGcgcg                                             >  1:868946/1‑149 (MQ=255)
                                                                                                          tttACTTATCCTGAAAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCCGGTGGTGATCGTCGCCGGGTAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCgg                                            <  1:4577399/148‑1 (MQ=255)
                                                                                                          aTTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCgg                                            >  2:3012358/1‑149 (MQ=255)
                                                                                                              tttATCATGAAAATTTACCGG‑TTATTCAGAAAAAACAGGACATGCTCTAAGCGATTCGTGATCACCAGGTGGTGTTGGTCGCCGGGGAAAGGGGTTCTGTTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGAtt                                        <  2:4492143/148‑1 (MQ=255)
                                                                                                               ttATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTa                                       >  1:964934/1‑149 (MQ=255)
                                                                                                                 aTCCTGACAATTTACCGG‑TTAGTCAGAAAAAACTGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCGGGGGAAAGGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTaaa                                     <  1:1909000/149‑1 (MQ=255)
                                                                                                                 aTCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTaaa                                     >  1:4659573/1‑149 (MQ=255)
                                                                                                                 aTCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTaaa                                     >  2:3957504/1‑149 (MQ=255)
                                                                                                                 aTCCTGAAAATTTACCGT‑TTAGTCATAAAAAACAGGACATTCTCGAAGCTATTCGTGATCACCTGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTaaa                                     <  1:2489811/149‑1 (MQ=255)
                                                                                                                  tCCTGACATTTTACCGG‑TTAGTCAGAAAAAACCGGACATTCTCGAAGCGATTCGTGATCACCCGGTGGTGATCGTCGCCGGGGAAAGGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAg                                    <  2:2029876/149‑1 (MQ=255)
                                                                                                                  tCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAg                                    >  2:455678/1‑149 (MQ=255)
                                                                                                                  tCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAg                                    >  1:2579211/1‑149 (MQ=255)
                                                                                                                     tGAAAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGCTTCGTGATCACCAGGTGGGGATCGTCGCCGGGTAACCGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGAc                                 <  2:3262200/149‑1 (MQ=255)
                                                                                                                      gaaaaTTTACGGG‑TTAGACAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCTGGTGGTTATCGTCGCCGGGTAAAGGGGTTCTGTAAACACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACt                                <  1:1760109/146‑1 (MQ=255)
                                                                                                                         attttACCGG‑TTCGTCAGAAAAAACAGGCCATTCTCGAAGCGATTCGTGATCACCCGGTGGGGATCGTCGCCGGGGAACCGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGAt                             <  1:288337/147‑1 (MQ=255)
                                                                                                                         aaTTTACCGT‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCGGTATGGAGCTGGGGCGCGGGATTAAAGGACTGAt                             <  2:61462/149‑1 (MQ=255)
                                                                                                                          aTTTTCCGGTTTAGTCATAAAAAACCGGAACTGCTCTACGCGAGTCGTGATCACCTGGGGGTGATGGGCGCCGGGGAACGGGGTTGTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGAt                             <  1:2042489/149‑1 (MQ=255)
                                                                                                                          aTTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATc                            >  1:3221444/1‑149 (MQ=255)
                                                                                                                           tttACCGG‑TTATTCACAAAAAACAGGACATTCCCGATGCGATTCGTGATCACCAGGTGGTGATCGTCGCGGGGTAAACGGGTAGTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCg                           <  1:533716/149‑1 (MQ=255)
                                                                                                                           tttACCGG‑TTAGTCAGAAAAAACAGAACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCg                           >  1:1054858/1‑149 (MQ=255)
                                                                                                                                      aGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCT‑GGGCGCGGGATTAAAGGACTGATCGGCCATACCCAg                >  2:216009/1‑149 (MQ=255)
                                                                                                                                        tCAGAAAAAACAGAACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGc               >  2:3126035/1‑149 (MQ=255)
                                                                                                                                           gAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCcgc            >  1:1380158/1‑149 (MQ=255)
                                                                                                                                            aaaaaaCAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCcgcg           >  1:2182315/1‑149 (MQ=255)
                                                                                                                                             aaaaaCAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGcgt          >  2:1365024/1‑149 (MQ=255)
                                                                                                                                              aaaaCAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTAACGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGcgtc         >  1:3932975/1‑149 (MQ=255)
                                                                                                                                                aaCAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGcgtcgt       >  2:3457870/1‑149 (MQ=255)
                                                                                                                                                 aCAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCgtcgtc      <  2:1827839/149‑1 (MQ=255)
                                                                                                                                                  cAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCt     >  2:292213/1‑149 (MQ=255)
                                                                                                                                                  cAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCt     >  2:1745703/1‑149 (MQ=255)
                                                                                                                                                   aGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTg    >  2:3575528/1‑149 (MQ=255)
                                                                                                                                                   aGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTg    >  2:1132629/1‑149 (MQ=255)
                                                                                                                                                     gACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTggc  >  2:4651221/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GAAGGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGG‑TTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGC  >  CP009273/1477422‑1477718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A