Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 255,284 G→T 32.2% N176K (AAC→AAA)  phoE ← outer membrane phosphoporin protein E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273255,2840GT32.2% 445.7 / 192.2 240N176K (AAC→AAA) phoEouter membrane phosphoporin protein E
Reads supporting (aligned to +/- strand):  ref base G (94/68);  new base T (49/29);  total (143/97)
Fisher's exact test for biased strand distribution p-value = 5.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 244 total aligned reads displayed.

TGCCTGTGCCACGGCTTTGCAGGTTCTGCTCGTTGGTGCGATCTGAGTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATC  >  CP009273/255136‑255433
                                                                                                                                                    |                                                                                                                                                     
   cTGTGCCACGGCTTTGCAGGTTCTGCTCGTTGGTGCGATCTGAGTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGtttt                                                                                                                                                   <  2:2074160/150‑1 (MQ=255)
    tgtgCCACGGCTTTGCAGGTTCTGCTCGTTGGTGCGATCTGAGTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCtttttt                                                                                                                                                  <  2:1300077/150‑1 (MQ=255)
    tgtgCCACGGCTTTGCAGGTTCTGCTCGTTGGTGCGATCTGAGTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGttttt                                                                                                                                                  <  2:3804433/150‑1 (MQ=255)
    tgtgCCACGGCTTTGCAGGTTCTGCTCGTTGGTGCGATCTGAGTTGGTATAGGCCACACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGttttt                                                                                                                                                  <  2:2945781/150‑1 (MQ=255)
     gtgCCACGGCTTTGCAGGTTCTGCTCGTTGGTGCGATCTGAGTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTc                                                                                                                                                 <  1:1581818/150‑1 (MQ=255)
        ccACGGCTTTGCAGGTTCTGCTCGTTGGTGCGATCTGAGTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGGTTTCGTTTTTCCCt                                                                                                                                              >  2:1092218/1‑150 (MQ=255)
         cACGGCTTTGCAGGTTCTGCTCGTTGGTGCGATCTGAGTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCTTTTTTCCCtt                                                                                                                                             <  2:417319/150‑1 (MQ=255)
         cACGGCTTTGCAGGTTCTGCTCGTTGGTGCGATCTGAGTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCtt                                                                                                                                             >  2:1656082/1‑150 (MQ=255)
         cACGGCTTTGCAGGTTCTGCTCGTTGGTGCGATCTGAGTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAAAGTCGCGGTTTTCGTTTTTcccgt                                                                                                                                             >  1:3170521/1‑148 (MQ=255)
         cACGGCTTTGCAGGTTCTGCTCGTTGGTGCGATCTGAGTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTGTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCtt                                                                                                                                             >  2:929446/1‑150 (MQ=255)
          aCGGCTTTGCAGGTTCTGCTCGTTGGTGCGATCTGAGTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTg                                                                                                                                            <  1:3529409/150‑1 (MQ=255)
           cGGCTTTGCAGGTTCTGCTCGTTGGTGCGATCTGAGTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCTTTTTTCCCTTGa                                                                                                                                           >  1:4198823/1‑150 (MQ=255)
              cTTTGCAGGTTCTGCTCGTTGGTGCGATCTGAGTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGatat                                                                                                                                        <  1:103775/150‑1 (MQ=255)
              cTTTGCAGGTTCTGCTCGTTGGTGCGATCTGAGTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTGTTGCTTTTTAACGTCGCGGTTTTATTTTTTACCGTGttat                                                                                                                                        >  1:425884/1‑150 (MQ=255)
                ttGCAGGTTCTGCTCGTTGGTGCTATCTGAGTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCAGCCTAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTTATATTg                                                                                                                                      <  2:1458077/150‑1 (MQ=255)
                ttGCAGGTTCTGCTCGTTGGTGCGATCTGAGTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCTTTTTTCCCTTGATATTg                                                                                                                                      <  1:3169032/150‑1 (MQ=255)
                ttGCAGGTTCTGCTCGTTGGTGCGATCTGAGTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTg                                                                                                                                      <  2:3203189/150‑1 (MQ=255)
                    aGGTTCTGCTCGTTGGTGCGATCTGAGTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCTTTTTTCCCTTGATATTGCAgg                                                                                                                                  >  1:3038594/1‑150 (MQ=255)
                    aGGTTCTGCTCGTTGGTGCGATCTGAGTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTTCAgg                                                                                                                                  >  1:2457470/1‑150 (MQ=255)
                     ggTTCTGCTCGTTGGTGCGATCTGAGTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAggg                                                                                                                                 >  2:301177/1‑150 (MQ=255)
                     ggTTCTGCTCGTTGGTGCGATCTGAGTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTACCTTGATATTGCAggg                                                                                                                                 >  2:2821559/1‑150 (MQ=255)
                      gTTCTGCTCGTTGGTGCGATCTGAGTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGt                                                                                                                                <  1:1430254/150‑1 (MQ=255)
                          tGCTCGTTGGTGCGATCTGAGTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCTTTTTTCCCTTGATATTGCAGGGTTAAg                                                                                                                            <  1:4467906/150‑1 (MQ=255)
                               gTTGGTGCGATCTGAGTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAg                                                                                                                       >  1:1307913/1‑150 (MQ=255)
                               gTTGGTGCGATCTGAGTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGGTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAggtaag                                                                                                                       >  2:1157297/1‑145 (MQ=255)
                                  ggTGCGATCTGAGTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTGTTGCTTTTTAACGTCGCGGTTTTCGTTTTTGCCTTGATATTGAAGGGTTAAGTTAAGGcc                                                                                                                    >  1:3166879/1‑150 (MQ=255)
                                    tGCGATCTGAGTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCAt                                                                                                                  <  2:396664/150‑1 (MQ=255)
                                    tGCGATCTGAGTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCAt                                                                                                                  <  2:2640470/150‑1 (MQ=255)
                                        aTCTGAGTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGAt                                                                                                              >  2:363555/1‑150 (MQ=255)
                                             atttGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTAAGGCCATCGATAACGc                                                                                                         >  1:2799874/3‑150 (MQ=255)
                                              gTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGcc                                                                                                        >  2:1257555/1‑150 (MQ=255)
                                              gTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGcc                                                                                                        >  2:895823/1‑150 (MQ=255)
                                              gTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTACCTTGATATTTCAGGGTTAAGTTAAGGCCATCGATAACGcc                                                                                                        >  2:1257673/1‑150 (MQ=255)
                                               ttGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCTTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCg                                                                                                       >  2:4535813/1‑150 (MQ=255)
                                                 ggTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGGCGCGGTTTTCTTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCCTCGATAACGCCgaa                                                                                                     >  1:622063/1‑150 (MQ=255)
                                                 ggTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAAAGTCGCGGTTTTAGTTTTTCCCGTGATATTGCAGGGTTAAGTTCAGGCCCTCGATAACCCCgaa                                                                                                     >  1:2088316/1‑150 (MQ=255)
                                                         ccccACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAAAGTCGCGGTTTTCTTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCCTCGATAACGCCGAAGAAGTCgg                                                                                             >  2:1598585/1‑150 (MQ=255)
                                                             aCTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGtt                                                                                         <  2:2137847/150‑1 (MQ=255)
                                                              cTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGGTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGtta                                                                                        >  1:2133871/1‑149 (MQ=255)
                                                               tAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCTTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTcc                                                                                       >  2:928459/1‑150 (MQ=255)
                                                               tAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTcc                                                                                       <  1:992675/150‑1 (MQ=255)
                                                                aaTGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCg                                                                                      <  2:4631791/150‑1 (MQ=255)
                                                                 aTGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGa                                                                                     <  2:2233968/150‑1 (MQ=255)
                                                                      gAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGGTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGt                                                                                >  2:587415/1‑150 (MQ=255)
                                                                      gAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCGTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGt                                                                                >  2:3154471/1‑150 (MQ=255)
                                                                      gAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCATGATATTGCAGGGGTAAGTTAAGGCCATCGAGAACGCCGAAGAATTCGGTGGTACGATAAGt                                                                                >  1:170679/1‑150 (MQ=255)
                                                                      gAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCGTTTTAAAGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTAAGGCCCTCGATAACGCCCAAGAAGTCGGTGTTCCGATACGt                                                                                >  1:1282572/1‑150 (MQ=255)
                                                                        aaTCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGGTTTTTAACGTCGCGGTTTTGTTTTTTGCCTTGGTATTGCAGGGTTTAGTTCAGGCCATCGATAAAGCCGAAGAAGTCGGTGTTCCGATACGTCg                                                                              >  1:4004166/1‑150 (MQ=255)
                                                                        aaTCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCTTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCg                                                                              <  1:1821556/150‑1 (MQ=255)
                                                                        aaTCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCTTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCg                                                                              <  1:2302948/150‑1 (MQ=255)
                                                                        aaTCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCTTTTTTACCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTACGATACGTCg                                                                              >  1:4040205/1‑150 (MQ=255)
                                                                        aaTCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCCTCGATAACGCCGAAGAAGTCGGTGTTACGATACGTCg                                                                              >  1:503338/1‑150 (MQ=255)
                                                                        aaTCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTGTTGCTTTTTAACGTCGCGGTTTTCTTTTTTACCTTGATATTGCAGGGGTAAGTTCAGGCCATCGATAAAGCCGAAGAAGTCGGTGTTCCGATACGGCg                                                                              >  1:2726936/1‑150 (MQ=255)
                                                                          tCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGcc                                                                            >  2:1834501/1‑150 (MQ=255)
                                                                          tCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGcc                                                                            <  1:1773727/150‑1 (MQ=255)
                                                                          tCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGcc                                                                            >  2:2893884/1‑150 (MQ=255)
                                                                                  gccAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCt                                                                    <  2:3052046/150‑1 (MQ=255)
                                                                                  gccAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCt                                                                    <  2:4223490/150‑1 (MQ=255)
                                                                                   ccAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCTTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTg                                                                   <  1:2418527/150‑1 (MQ=255)
                                                                                    cAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCTTTTTTCCCGTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTgg                                                                  >  2:3887651/1‑150 (MQ=255)
                                                                                         tCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGt                                                             >  2:2480510/1‑150 (MQ=255)
                                                                                          cATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGtt                                                            <  1:3933753/150‑1 (MQ=255)
                                                                                           atatGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGGTTTCTTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGttt                                                           >  2:8686/1‑150 (MQ=255)
                                                                                              tGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCTTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATAAGTCGCCAGACCGCTGGCGCGTTTGGt                                                        >  1:2572455/1‑150 (MQ=255)
                                                                                                tCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCTTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCa                                                      <  1:3615422/150‑1 (MQ=255)
                                                                                                tCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCa                                                      <  1:2654811/150‑1 (MQ=255)
                                                                                                  aaTGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATa                                                    >  2:2655917/1‑150 (MQ=255)
                                                                                                    tGACGTGCCGAAGCCATCGCCGTTTTGGTTTTTAACGTCGCGGTTTTATTTTTTACCATTATAATGCAGGGTTAAGTTCAGGCCAACGATAACGCCGAAGAAGTCGGTGTTACGATAAGTCGCCAGACCGCTGGCGCGGTTGGTCATaaa                                                  >  1:2477207/1‑150 (MQ=255)
                                                                                                    tGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGGGGTTTTGTTTTTTACCTTTTTAATGCAGGGTTAAGTTCAAGCCATAGATAACACCGAAGAAGTAGGTTTTACCATAAGACACCAGAACGCAGTCGCGTTTGGTAATaaa                                                  >  1:2054636/1‑150 (MQ=255)
                                                                                                    tGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATcaa                                                  >  2:2634588/1‑150 (MQ=255)
                                                                                                     gACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCTTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAg                                                 >  2:2958240/1‑150 (MQ=255)
                                                                                                       cGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTTCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGtt                                               >  2:2750106/1‑150 (MQ=255)
                                                                                                          gCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCTTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTc                                            <  1:3449886/150‑1 (MQ=255)
                                                                                                           ccGAAGCCATCGCCGTTTTTCTTTTTAAAGTCGCGGTTTTCGTTTTTGCCTTGATATTGCAGGGTTAATTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTACGATACGTCGCCAGACCGCAGGCGCGTTTGGTAATAAAGttttag                                           >  1:3330286/1‑146 (MQ=255)
                                                                                                            cGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCTTTTTTCCCTTGATATTTCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCgg                                          >  2:3274785/1‑150 (MQ=255)
                                                                                                             gAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGt                                         >  2:1739130/1‑150 (MQ=255)
                                                                                                             gAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCGGGCGCGTTTGGTCATAAAGTTGTTGGt                                         >  2:1506783/1‑150 (MQ=255)
                                                                                                               aGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCTTTTTTCCCTTGATATTTCAGGGTTAAGTTCAGGCCCTCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCt                                       >  2:3674264/1‑150 (MQ=255)
                                                                                                                  cATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCTTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTgcg                                    <  1:3119557/150‑1 (MQ=255)
                                                                                                                  cATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCTTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTgcg                                    <  1:3792645/150‑1 (MQ=255)
                                                                                                                  cATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCTTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTgcg                                    >  2:4171681/1‑150 (MQ=255)
                                                                                                                     cGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCg                                 <  2:4406024/150‑1 (MQ=255)
                                                                                                                      gCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGa                                >  1:1529408/1‑150 (MQ=255)
                                                                                                                       ccGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAg                               >  2:1396857/1‑150 (MQ=255)
                                                                                                                          ttttGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGac                            >  1:2649941/1‑149 (MQ=255)
                                                                                                                            ttGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCAATCGATAACGACGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATc                          <  2:19025/150‑1 (MQ=255)
                                                                                                                             tGCTTTTGACCGTCGCGGCTTTCGTTTTTCCCTTGATTTTGCCGGGTTAAGTTCAGGCCATCGATAACGCCGATGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGTTCTGCGCCGAGGAATCg                         <  2:4250966/150‑1 (MQ=255)
                                                                                                                               cTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGcc                       >  2:666004/1‑150 (MQ=255)
                                                                                                                                  tttAACGTCGCGGTTTTCTTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGccacc                    >  2:4484930/1‑150 (MQ=255)
                                                                                                                                   ttAACGTCGCGGTTTTCTTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGccacca                   <  1:1886885/150‑1 (MQ=255)
                                                                                                                                     aaCGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCaaa                 >  1:4076699/1‑150 (MQ=255)
                                                                                                                                     aaCGTCGCGGTTTTCGTTTTTCCCGTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCcaa                 >  1:3883913/1‑150 (MQ=255)
                                                                                                                                        gTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTc              >  1:754450/1‑150 (MQ=255)
                                                                                                                                        gTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTc              <  1:2500127/150‑1 (MQ=255)
                                                                                                                                         tCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTcc             <  1:3424722/150‑1 (MQ=255)
                                                                                                                                            cgGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCggg          >  2:1939129/1‑150 (MQ=255)
                                                                                                                                                tttCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACa      >  2:435866/1‑150 (MQ=255)
                                                                                                                                                tttCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACa      >  2:3582664/1‑150 (MQ=255)
                                                                                                                                                  tCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACata    <  2:2373321/150‑1 (MQ=255)
                                                                                                                                                    gTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATc  >  2:344563/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TGCCTGTGCCACGGCTTTGCAGGTTCTGCTCGTTGGTGCGATCTGAGTTGGTATAGGCCCCACTAATGGCGAAATCGCTGCCGCCAAAGTCATATGTCAATGACGTGCCGAAGCCATCGCCGTTTTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATC  >  CP009273/255136‑255433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A