Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 263,270 C→A 14.9% V149V (GTG→GTT)  yafZ ← CP4‑6 prophage; conserved protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273263,2700CA14.9% 869.0 / 85.7 265V149V (GTG→GTT) yafZCP4‑6 prophage; conserved protein
Reads supporting (aligned to +/- strand):  ref base C (111/114);  new base A (24/16);  total (135/130)
Fisher's exact test for biased strand distribution p-value = 2.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 265 total aligned reads displayed.

CCGGCTGGTGGTCTTCACCAAAGCGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGTTGAGTAGAATAATT  >  CP009273/263122‑263417
                                                                                                                                                    |                                                                                                                                                   
   gCTGGTGGTCTTCACCAAAGCGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCt                                                                                                                                                 >  2:2163601/1‑150 (MQ=255)
   gCTGGTGGTCTTCACCAAAGCGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCt                                                                                                                                                 <  1:3465382/150‑1 (MQ=255)
        tggtCTTCACCAAAGCGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTc                                                                                                                                            <  2:3358112/150‑1 (MQ=255)
         ggtCTTCACCAAAGCGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTca                                                                                                                                           >  1:4147989/1‑150 (MQ=255)
         ggtCTTCACCAAAGCGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTca                                                                                                                                           >  1:2630280/1‑150 (MQ=255)
          gtCTTCACCAAAGCGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTcac                                                                                                                                          >  1:1015727/1‑150 (MQ=255)
            cTTCACCAAAGCGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTcacca                                                                                                                                        <  1:843830/150‑1 (MQ=255)
               cACCAAAGCGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATAACCTGACTCACCACGt                                                                                                                                     <  1:3536202/150‑1 (MQ=255)
                aCCAAAGCGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTc                                                                                                                                    >  2:3947498/1‑150 (MQ=255)
                 ccAAAGCGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTcc                                                                                                                                   <  2:551171/150‑1 (MQ=255)
                 ccAAAGCGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTcc                                                                                                                                   <  2:4238098/150‑1 (MQ=255)
                  cAAAGCGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTccc                                                                                                                                  <  2:1712849/150‑1 (MQ=255)
                  cAAAGCGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTccc                                                                                                                                  >  1:1914400/1‑150 (MQ=255)
                   aaaGCGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTcccc                                                                                                                                 >  2:3277105/1‑150 (MQ=255)
                   aaaGCGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTcccc                                                                                                                                 >  2:808261/1‑150 (MQ=255)
                    aaGCGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATAACCTGACTCACCACGTccccc                                                                                                                                <  1:702822/150‑1 (MQ=255)
                       cGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTtg                                                                                                                             <  2:2309382/150‑1 (MQ=255)
                         gTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTtgtg                                                                                                                           <  2:1038960/150‑1 (MQ=255)
                           aTGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTtgtgtg                                                                                                                         >  1:2477561/1‑150 (MQ=255)
                           aTGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTtgtgtg                                                                                                                         >  2:4098125/1‑150 (MQ=255)
                               gAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCAc                                                                                                                     >  2:3474637/1‑150 (MQ=255)
                               gAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATAACCTGACTCACCACGTCCCCCTTGTGTGGCAc                                                                                                                     >  1:471741/1‑150 (MQ=255)
                                aGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATAACCTGACTCACCACGTCCCCCTTGTGTGGCAcc                                                                                                                    >  2:2779951/1‑150 (MQ=255)
                                     ggcTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCAc                                                                                                               >  2:2332118/1‑150 (MQ=255)
                                     ggcTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATAACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCAc                                                                                                               >  1:4362737/1‑150 (MQ=255)
                                             ccAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCaa                                                                                                       >  2:3134566/1‑150 (MQ=255)
                                              cAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGAACCCGCACCTCGCCaaa                                                                                                      >  2:2983690/1‑150 (MQ=255)
                                               aGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAAc                                                                                                     >  2:1236621/1‑150 (MQ=255)
                                                gTGCCTGCTGTGCGGGGGGGGTCAAAAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCTGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACg                                                                                                    <  1:1612170/150‑1 (MQ=18)
                                                gTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACg                                                                                                    <  2:3440124/150‑1 (MQ=255)
                                                 tGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATACCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGa                                                                                                   >  1:4064849/1‑150 (MQ=255)
                                                   ccTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACt                                                                                                 <  1:370560/150‑1 (MQ=255)
                                                         gtgCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGc                                                                                           <  1:1542130/150‑1 (MQ=255)
                                                          tgCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCa                                                                                          <  2:4519670/150‑1 (MQ=255)
                                                          tgCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCa                                                                                          >  2:1089116/1‑150 (MQ=255)
                                                             cGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGAc                                                                                       >  1:392514/1‑150 (MQ=255)
                                                             cGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGAc                                                                                       >  1:4537310/1‑150 (MQ=255)
                                                             cGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGAc                                                                                       <  1:2964762/150‑1 (MQ=255)
                                                               ggggTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGa                                                                                     >  1:1265468/1‑150 (MQ=255)
                                                                  gTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGcc                                                                                  >  2:3895676/1‑150 (MQ=255)
                                                                      cAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGtt                                                                              >  2:2033582/1‑150 (MQ=255)
                                                                      cAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGtt                                                                              >  2:2263979/1‑150 (MQ=255)
                                                                       aaCAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTc                                                                             >  1:2206658/1‑150 (MQ=255)
                                                                        aCAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATAACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCt                                                                            >  2:2746758/1‑150 (MQ=255)
                                                                        aCAGCAACGACTGCATGGCATCCCGTTTATCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCt                                                                            >  1:3174961/1‑150 (MQ=255)
                                                                         cAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTg                                                                           <  2:2890286/150‑1 (MQ=255)
                                                                          aGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGa                                                                          >  2:846140/1‑150 (MQ=255)
                                                                          aGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGa                                                                          >  1:845615/1‑150 (MQ=255)
                                                                          aGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGa                                                                          >  1:3596727/1‑150 (MQ=255)
                                                                             aaCGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACaa                                                                       >  1:1002961/1‑150 (MQ=255)
                                                                               cGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAAc                                                                     >  1:4449121/1‑150 (MQ=255)
                                                                                   tGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATAACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCa                                                                 <  2:911836/150‑1 (MQ=255)
                                                                                     cATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACg                                                               <  1:1374164/150‑1 (MQ=255)
                                                                                         gCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAAt                                                           <  2:1796788/150‑1 (MQ=255)
                                                                                           aTCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAg                                                         >  2:2492225/1‑150 (MQ=255)
                                                                                              ccGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTcc                                                      <  2:4341276/150‑1 (MQ=255)
                                                                                              ccGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATAACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTcc                                                      >  1:4027020/1‑150 (MQ=255)
                                                                                               cGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTccc                                                     <  2:2390619/150‑1 (MQ=255)
                                                                                                 tttCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCgg                                                   >  1:4576878/1‑150 (MQ=255)
                                                                                                    ctcctcCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCaa                                                >  2:2172854/1‑150 (MQ=255)
                                                                                                     tcctccACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAAc                                               >  1:2533499/1‑150 (MQ=255)
                                                                                                         ccACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCt                                           <  2:4136902/150‑1 (MQ=255)
                                                                                                          cACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTg                                          >  2:2575961/1‑150 (MQ=255)
                                                                                                          cACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTg                                          <  2:918973/150‑1 (MQ=255)
                                                                                                            cccGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGAt                                        <  1:4557563/150‑1 (MQ=255)
                                                                                                             ccGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATa                                       <  2:3304122/150‑1 (MQ=255)
                                                                                                             ccGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATAACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATa                                       >  1:1960101/1‑150 (MQ=255)
                                                                                                                  cAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAAc                                  >  1:2844205/1‑150 (MQ=255)
                                                                                                                  cAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAAc                                  >  1:2908231/1‑150 (MQ=255)
                                                                                                                   aaaaaTCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACt                                 <  1:681935/150‑1 (MQ=255)
                                                                                                                   aaaaaTCCACAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCGCCTTTTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACTAACt                                 <  2:5304/150‑1 (MQ=255)
                                                                                                                     aaaTCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATGCGAACTgg                               <  1:3820627/150‑1 (MQ=255)
                                                                                                                      aaTCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGt                              >  1:1719479/1‑150 (MQ=255)
                                                                                                                       aTCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGtt                             >  1:595792/1‑150 (MQ=255)
                                                                                                                       aTCCCCAGCACCTCATACGCGCCTTCAATAACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGtt                             >  2:1292842/1‑150 (MQ=255)
                                                                                                                        tCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTc                            >  2:2211478/1‑150 (MQ=255)
                                                                                                                         ccccAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTcc                           <  1:2182513/150‑1 (MQ=255)
                                                                                                                          cccAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCa                          <  2:1948853/150‑1 (MQ=255)
                                                                                                                            cAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATc                        >  2:2825430/1‑150 (MQ=255)
                                                                                                                            cAGCAACTCATACGCGCCTTCAATAACATGACTCACCACGTCCCCCTTGTTTGGCACCCGAACCTCTCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCAAGTCAACATCTGATACGAAATGGTTCCATc                        >  2:4478321/1‑150 (MQ=255)
                                                                                                                             aGCACCTCATACGCGCCTTCAATAACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCg                       >  1:2723111/1‑150 (MQ=255)
                                                                                                                              gCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGt                      <  1:3060278/150‑1 (MQ=255)
                                                                                                                              gCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGt                      <  1:2328502/150‑1 (MQ=255)
                                                                                                                               cACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTg                     >  1:4242799/1‑150 (MQ=255)
                                                                                                                               cACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTg                     <  1:3363620/150‑1 (MQ=255)
                                                                                                                                aCCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTga                    >  1:3111793/1‑150 (MQ=255)
                                                                                                                                aCCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTga                    <  1:2667166/150‑1 (MQ=255)
                                                                                                                                    cATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGt                >  1:3638268/1‑150 (MQ=255)
                                                                                                                                    cATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGt                <  2:1575874/150‑1 (MQ=255)
                                                                                                                                     aTACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCACCATCTGATACGAACTGGTTCCATCGTGAGAGtt               >  1:900187/1‑150 (MQ=255)
                                                                                                                                     aTACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGtt               >  1:3726112/1‑150 (MQ=255)
                                                                                                                                     aTACGCGCCTTCAATAACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGtt               >  2:3135607/1‑150 (MQ=255)
                                                                                                                                      tACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAATCGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGTTg              >  2:2626352/1‑150 (MQ=255)
                                                                                                                                         gcgcCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGTTGAGt           <  2:3436608/150‑1 (MQ=255)
                                                                                                                                           gcCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGTTGAGTAg         <  2:3717263/150‑1 (MQ=255)
                                                                                                                                            ccTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGTTGAGTAGa        <  1:1758680/150‑1 (MQ=255)
                                                                                                                                            ccTTCAATAACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGTTGAGTAGa        <  2:1112497/150‑1 (MQ=255)
                                                                                                                                                cAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGTTGAGTAGaataa    <  1:983976/150‑1 (MQ=255)
                                                                                                                                                 aaTCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGTTGAGTAGaataat   <  1:1261091/150‑1 (MQ=255)
                                                                                                                                                  aTCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGTTGAGTAGAATAAtt  <  1:660820/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCGGCTGGTGGTCTTCACCAAAGCGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGTTGAGTAGAATAATT  >  CP009273/263122‑263417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A