Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 876,738 G→T 14.8% D186Y (GAT→TAT)  dacC → D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 6a)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273876,7380GT14.8% 750.2 / 77.4 229D186Y (GAT→TAT) dacCD‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 6a)
Reads supporting (aligned to +/- strand):  ref base G (111/84);  new base T (19/15);  total (130/99)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

Only 100 of 245 total aligned reads displayed.

CCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGA  >  CP009273/876589‑876885
                                                                                                                                                     |                                                                                                                                                   
  tGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGat                                                                                                                                                   <  1:2009696/150‑1 (MQ=255)
   gTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGata                                                                                                                                                  <  1:1071229/150‑1 (MQ=255)
   gTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGata                                                                                                                                                  <  1:2932147/150‑1 (MQ=255)
    tATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCTatat                                                                                                                                                 >  2:3928932/1‑150 (MQ=255)
     aTTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATg                                                                                                                                                <  2:3854126/150‑1 (MQ=255)
       tGCTCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGTTGAAGGGTTATGCCAAAAAACTGGGTCTGACCTACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCTATATGGc                                                                                                                                              <  2:126492/150‑1 (MQ=255)
                 gATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGt                                                                                                                                    >  1:220701/1‑150 (MQ=255)
                 gATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGt                                                                                                                                    >  1:58730/1‑150 (MQ=255)
                  aTTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTa                                                                                                                                   >  2:2262925/1‑150 (MQ=255)
                  aTTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTa                                                                                                                                   >  2:3862653/1‑150 (MQ=255)
                   ttACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTaa                                                                                                                                  <  1:1450172/150‑1 (MQ=255)
                    tACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTaaa                                                                                                                                 >  1:4255585/1‑150 (MQ=255)
                         tGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCTATATGGCATTGCTGGGTAAAGCAtt                                                                                                                            >  2:1312958/1‑150 (MQ=255)
                         tGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCAtt                                                                                                                            >  1:2001506/1‑150 (MQ=255)
                          gCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTg                                                                                                                           <  2:4411332/150‑1 (MQ=255)
                            cGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCTATATGGCATTGCTGGGTAAAGCATTGAt                                                                                                                         >  1:3797549/1‑150 (MQ=255)
                             gggAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCTATATGGCATTGCTGGGTAAAGCATTGATc                                                                                                                        <  2:419071/150‑1 (MQ=255)
                             gggAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATc                                                                                                                        >  2:3447177/1‑150 (MQ=255)
                             gggAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATc                                                                                                                        >  2:1388221/1‑150 (MQ=255)
                              ggAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATcc                                                                                                                       <  2:3765566/150‑1 (MQ=255)
                               gAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCa                                                                                                                      <  1:572809/150‑1 (MQ=255)
                               gAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCa                                                                                                                      >  1:2411711/1‑150 (MQ=255)
                                    aGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCTATATGGCATTGCTGGGTAAAGCATTGATCCACGAtg                                                                                                                 <  1:1764839/150‑1 (MQ=255)
                                         tCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCg                                                                                                            >  1:3266052/1‑150 (MQ=255)
                                          cATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCgg                                                                                                           >  1:6577/1‑150 (MQ=255)
                                            tttATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGaa                                                                                                         <  1:923699/150‑1 (MQ=255)
                                            tttATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGaa                                                                                                         <  1:3317099/150‑1 (MQ=255)
                                             ttATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAag                                                                                                        <  2:3552364/150‑1 (MQ=255)
                                              tATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAaga                                                                                                       >  1:1613524/1‑150 (MQ=255)
                                                ttGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCTATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGt                                                                                                     >  1:3663012/1‑150 (MQ=255)
                                                ttGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGt                                                                                                     <  2:2334449/150‑1 (MQ=255)
                                                ttGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGAACGGCCTGGATGCGCCGGGGCAGTACAGCACAGCGCGCTATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAagaga                                                                                                     >  1:3661222/1‑149 (MQ=255)
                                                    tCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGc                                                                                                 >  2:1491875/1‑150 (MQ=255)
                                                     cTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCTATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGcc                                                                                                >  2:1412709/1‑150 (MQ=255)
                                                      tgatgaATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCTATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCa                                                                                               >  1:963506/1‑150 (MQ=255)
                                                         tgaATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTc                                                                                            >  2:2455445/1‑150 (MQ=255)
                                                           aaTGGTTTTGCAAAAACACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGATGTTCAGCACCGCGCGCTATATGGCATTGCTGGGTAAAGCATTGATCCACGTTGTGCCGGAAGAGTACGCCATTCAt                                                                                          <  1:478533/150‑1 (MQ=255)
                                                            aTGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATa                                                                                         <  2:995071/150‑1 (MQ=255)
                                                            aTGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATa                                                                                         <  2:2831342/150‑1 (MQ=255)
                                                             tGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATaa                                                                                        >  1:877889/1‑150 (MQ=255)
                                                             tGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATaa                                                                                        >  2:4588151/1‑150 (MQ=255)
                                                              ggTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATaaa                                                                                       >  1:183768/1‑150 (MQ=255)
                                                              ggTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATaaa                                                                                       <  1:3124298/150‑1 (MQ=255)
                                                                ttATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATaaaga                                                                                     >  1:4125101/1‑150 (MQ=255)
                                                                 tATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATaaagag                                                                                    >  2:3233645/1‑150 (MQ=255)
                                                                   tGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATaaagagaa                                                                                  >  2:3641499/1‑150 (MQ=255)
                                                                    gCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATaaagagaaa                                                                                 <  2:314598/150‑1 (MQ=255)
                                                                     ccAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATaaagagaaag                                                                                >  2:2394003/1‑150 (MQ=255)
                                                                      cAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATaaagagaaaga                                                                               >  1:1534207/1‑150 (MQ=255)
                                                                      cAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATaaagagaaaga                                                                               <  1:3892977/150‑1 (MQ=255)
                                                                       aaaaaaCTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATaaagagaaagag                                                                              <  2:4068678/150‑1 (MQ=255)
                                                                       aaaaaaCTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGAAGTGCCGGAAGAGTACGCCAATCATaaagagaaagag                                                                              >  1:3734705/1‑150 (MQ=255)
                                                                        aaaaaCTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGt                                                                             >  2:898623/1‑150 (MQ=255)
                                                                        aaaaaCTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGt                                                                             >  2:631692/1‑150 (MQ=255)
                                                                           aaCTGGGTCTGACCAACACTACCTTCCAGTCGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCa                                                                          <  2:1339858/150‑1 (MQ=255)
                                                                            aCTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCAc                                                                         <  2:782910/150‑1 (MQ=255)
                                                                             cTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCAcc                                                                        >  2:1831389/1‑150 (MQ=255)
                                                                             cTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCAcc                                                                        >  1:1695036/1‑150 (MQ=255)
                                                                              tGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCt                                                                       >  2:3258665/1‑150 (MQ=255)
                                                                               gggTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCtt                                                                      <  1:1133921/150‑1 (MQ=255)
                                                                                 gTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTca                                                                    >  1:2851746/1‑150 (MQ=255)
                                                                                 gTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTca                                                                    >  1:2406948/1‑150 (MQ=255)
                                                                                   cTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTcaac                                                                  >  1:3552477/1‑150 (MQ=255)
                                                                                   cTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTcaac                                                                  <  1:3329388/150‑1 (MQ=255)
                                                                                    tGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTcaaca                                                                 >  1:3232090/1‑150 (MQ=255)
                                                                                    tGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTcaaca                                                                 <  2:1171286/150‑1 (MQ=255)
                                                                                     gACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTcaacaa                                                                >  2:3372851/1‑150 (MQ=255)
                                                                                     gACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTcaacaa                                                                >  2:717608/1‑150 (MQ=255)
                                                                                       ccAACACTACCTTCCAGACTGTGCACGGCCTGGATGCGCCGGGGCATTTCAGCACCGCGCGCTATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACaaaa                                                              >  1:2106929/1‑150 (MQ=255)
                                                                                         aaCACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAAtt                                                            <  1:2999788/150‑1 (MQ=255)
                                                                                           cacTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCg                                                          >  2:2986321/1‑150 (MQ=255)
                                                                                             cTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCAATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTc                                                        <  2:1542451/150‑1 (MQ=255)
                                                                                                ccTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGc                                                     >  1:4034903/1‑150 (MQ=255)
                                                                                                ccTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGc                                                     >  2:2823424/1‑150 (MQ=255)
                                                                                                     cAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCtaac                                                <  1:2478672/150‑1 (MQ=255)
                                                                                                     cAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCtaac                                                <  2:1687062/150‑1 (MQ=255)
                                                                                                      aGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCtaacc                                               >  2:4331881/1‑150 (MQ=255)
                                                                                                            tGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCtaaccgtaacc                                         <  2:2150978/150‑1 (MQ=255)
                                                                                                            tGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCtaaccgtaacc                                         >  1:1286302/1‑150 (MQ=255)
                                                                                                             gCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCtaaccgtaaccg                                        <  2:1970554/150‑1 (MQ=255)
                                                                                                              cACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTaaccgtaaccgt                                       >  2:3714261/1‑150 (MQ=255)
                                                                                                               aCGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTc                                      >  1:1232129/1‑150 (MQ=255)
                                                                                                                     tGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCtgt                                <  2:1992412/150‑1 (MQ=255)
                                                                                                                      ggATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCtgtg                               >  1:4301228/1‑150 (MQ=255)
                                                                                                                      ggATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATACGTCAGCCTAACCGTAACCGTCTGCtgtg                               >  2:4624327/1‑150 (MQ=255)
                                                                                                                        aTGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGa                             <  2:611731/150‑1 (MQ=255)
                                                                                                                                gggCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAAt                     <  2:4464006/150‑1 (MQ=255)
                                                                                                                                  gCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCt                   <  2:3919754/150‑1 (MQ=255)
                                                                                                                                   cAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTg                  >  1:4497266/1‑150 (MQ=255)
                                                                                                                                     gTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGaa                >  2:3875722/1‑150 (MQ=255)
                                                                                                                                      ttCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAAt               <  1:856076/150‑1 (MQ=255)
                                                                                                                                      ttCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGNAACCGTCTGCTGTGGAGCAGCAATCTGAAt               <  1:4275032/150‑1 (MQ=255)
                                                                                                                                        cAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCACTCTGAATGt             >  2:539321/1‑150 (MQ=255)
                                                                                                                                              cgcgcgcgATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATgg       >  1:1048277/1‑150 (MQ=255)
                                                                                                                                              cgcgcgcgATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATgg       >  1:1047465/1‑150 (MQ=255)
                                                                                                                                              cgcgcgcgATATGGCAGTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATgg       <  2:2884985/150‑1 (MQ=255)
                                                                                                                                                cgcgcgATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCa     >  1:4117454/1‑150 (MQ=255)
                                                                                                                                                  cgcgATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATg   <  2:4510223/150‑1 (MQ=255)
                                                                                                                                                  cgcgATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATg   <  2:669787/150‑1 (MQ=255)
                                                                                                                                                   gcgATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGa  <  2:4086345/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAAAAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAGTTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGA  >  CP009273/876589‑876885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A