Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 890,555 G→T 43.5% D37Y (GAT→TAT)  potG → putrescine transporter subunit: ATP‑binding component of ABC superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273890,5550GT43.5% 288.5 / inf 287D37Y (GAT→TAT) potGputrescine transporter subunit: ATP‑binding component of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base G (78/84);  new base T (57/68);  total (135/152)
Fisher's exact test for biased strand distribution p-value = 7.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 302 total aligned reads displayed.

TATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAATGCTTGATGGCGTCGATTTGTCACAGGT  >  CP009273/890408‑890703
                                                                                                                                                   |                                                                                                                                                    
 aTGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGattatg                                                                                                                                                   >  1:3933150/1‑150 (MQ=255)
 aTGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGattatg                                                                                                                                                   >  2:1657708/1‑150 (MQ=255)
 aTGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGattatg                                                                                                                                                   <  1:1996959/150‑1 (MQ=255)
  tGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATTATGt                                                                                                                                                  >  1:3108309/1‑150 (MQ=255)
   ggTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTc                                                                                                                                                 >  2:288096/1‑150 (MQ=255)
        gcTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCt                                                                                                                                            <  1:3667811/150‑1 (MQ=255)
        gcTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCt                                                                                                                                            <  1:901460/150‑1 (MQ=255)
         cTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTg                                                                                                                                           <  2:110998/150‑1 (MQ=255)
           tGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTGAc                                                                                                                                         <  1:2760924/150‑1 (MQ=255)
           tGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGAc                                                                                                                                         <  1:2363057/150‑1 (MQ=255)
            gCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTGAcc                                                                                                                                        >  1:4316260/1‑150 (MQ=255)
             cacacaTTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTGACCa                                                                                                                                       >  1:206639/1‑150 (MQ=255)
              acacaTTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCAt                                                                                                                                      <  1:4573175/150‑1 (MQ=255)
                acaTTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTGACCATCt                                                                                                                                    >  2:615627/1‑150 (MQ=255)
                acaTTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCt                                                                                                                                    <  2:2961197/150‑1 (MQ=255)
                  aTTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTGACCATCTAc                                                                                                                                  <  1:252702/150‑1 (MQ=255)
                  aTTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTGACCATCTAc                                                                                                                                  <  1:2748813/150‑1 (MQ=255)
                   ttCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTGACCATCTACa                                                                                                                                 <  2:3918255/150‑1 (MQ=255)
                   ttCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACa                                                                                                                                 >  2:3691413/1‑150 (MQ=255)
                   ttCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACa                                                                                                                                 >  1:1433977/1‑150 (MQ=255)
                    tCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACaa                                                                                                                                <  1:425219/150‑1 (MQ=255)
                     cAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTGACCATCTACaaa                                                                                                                               >  1:3325725/1‑150 (MQ=255)
                     cAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACaaa                                                                                                                               <  1:2227953/150‑1 (MQ=255)
                      aaTGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTATAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTGACCATCTACaacg                                                                                                                              <  2:2592395/150‑3 (MQ=255)
                           cGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGaa                                                                                                                         >  1:402693/1‑150 (MQ=255)
                             gagaGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAAt                                                                                                                       <  2:241711/150‑1 (MQ=255)
                              agagCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTGACCATCTACAAAGGTGAAATc                                                                                                                      >  1:646875/1‑150 (MQ=255)
                              agagCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATc                                                                                                                      >  1:325331/1‑150 (MQ=255)
                              agagCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATc                                                                                                                      >  1:325687/1‑150 (MQ=255)
                              agagCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACAAGCGGTGGATTATGTCAGCCTGACCATCTACAAAGGTGAAATc                                                                                                                      <  1:2929689/150‑1 (MQ=255)
                               gagCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCt                                                                                                                     >  2:2660933/1‑150 (MQ=255)
                                agcagcCGTGACTGACGCTCTCCCTCGCCCGCAGGCGATATCCAGTTAGGCGCTGACTCCGCTATTAGACATCCGGAACCTGACCAAATCATACGATGGTCAACTTGCGGTTGATGATGTCAGCCTGACCATCTTCAAAGGTTAAATCtt                                                                                                                    <  2:4019463/150‑1 (MQ=255)
                                   agcCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTGACCATCTACAAAGGTGAAATCTTcgc                                                                                                                 >  2:490939/1‑150 (MQ=255)
                                   agcCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTcgc                                                                                                                 >  2:1147828/1‑150 (MQ=255)
                                    gcCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTGACCATCTACAAAGGTGAAATCTTcgcg                                                                                                                >  2:809111/1‑150 (MQ=255)
                                     ccGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCgcgc                                                                                                               >  2:445997/1‑150 (MQ=255)
                                       gTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCgctg                                                                                                             >  1:1204077/1‑150 (MQ=255)
                                            tGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTggg                                                                                                        >  2:1347559/1‑150 (MQ=255)
                                            tGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTggg                                                                                                        >  2:2770383/1‑150 (MQ=255)
                                             gACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGgc                                                                                                       <  1:3794362/150‑1 (MQ=255)
                                             gACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGgc                                                                                                       >  1:590169/1‑150 (MQ=255)
                                              aCGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGgcg                                                                                                      >  1:2705479/1‑150 (MQ=255)
                                              aCGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGgcg                                                                                                      <  2:1544794/150‑1 (MQ=255)
                                               cGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGgcgc                                                                                                     >  2:2335365/1‑150 (MQ=255)
                                                 cTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCAt                                                                                                   <  2:4117769/150‑1 (MQ=255)
                                                  tATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATc                                                                                                  >  2:1856286/1‑150 (MQ=255)
                                                     cccTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCgg                                                                                               >  2:2555584/1‑150 (MQ=255)
                                                       cTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCt                                                                                             >  2:450102/1‑150 (MQ=255)
                                                           cccGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTgg                                                                                         <  1:4452863/150‑1 (MQ=255)
                                                            ccGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGc                                                                                        <  1:1050655/150‑1 (MQ=255)
                                                            ccGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGc                                                                                        <  1:2261033/150‑1 (MQ=255)
                                                                aGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGt                                                                                    <  2:3386216/150‑1 (MQ=255)
                                                                  gCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTcc                                                                                  <  2:3850700/150‑1 (MQ=255)
                                                                  gCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTcc                                                                                  <  1:4183146/150‑1 (MQ=255)
                                                                     aaaaCCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACg                                                                               <  1:2909313/150‑1 (MQ=255)
                                                                       aaCCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACgct                                                                             <  2:659344/150‑1 (MQ=255)
                                                                           cGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGctgctg                                                                         >  2:333176/1‑150 (MQ=255)
                                                                               aGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTa                                                                     <  1:1450717/150‑1 (MQ=255)
                                                                                ggCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTAt                                                                    <  1:1505278/150‑1 (MQ=255)
                                                                                   gcTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCt                                                                 <  2:1399531/150‑1 (MQ=255)
                                                                                    cTGACGCCGCTATTAGAAATCCGAAACCTGACCAAATCCTACGATCGTCAACATGCGGAGGATTATGTAAGCCTGACAATCAAAAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCAGGCAGTGTCAAGTCAACGCTGCTGCGTATGCTg                                                                >  2:1004958/1‑150 (MQ=255)
                                                                                      gACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGc                                                              >  1:671112/1‑150 (MQ=255)
                                                                                      gACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGc                                                              >  1:1567338/1‑150 (MQ=255)
                                                                                      aaCGCCGCTATTAGAAATCCGCAACCTGACCAAATCCAACTATGGTCAACATGCGGTGGATTATGTCAGACTTACCAACTACAAAGGAGAAATCTTCGCGCACCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGc                                                              >  2:1466418/2‑150 (MQ=255)
                                                                                        cgccgcTATTAGAAATACGCAACCTGACCAAATCATACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAg                                                            >  2:301699/1‑150 (MQ=255)
                                                                                         gccgcTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTGACCATCTACAACGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAgg                                                           <  1:1838717/150‑1 (MQ=255)
                                                                                         gccgcTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAgg                                                           >  2:3135616/1‑150 (MQ=255)
                                                                                         gccgcTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAgg                                                           >  2:4305609/1‑150 (MQ=255)
                                                                                           cgcTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGtt                                                         <  2:3527453/150‑1 (MQ=255)
                                                                                            gcTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGttt                                                        <  1:1093772/150‑1 (MQ=255)
                                                                                                ttAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGaa                                                    <  2:1977093/150‑1 (MQ=255)
                                                                                                ttAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGaa                                                    >  1:4102929/1‑150 (MQ=255)
                                                                                                 tAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGaac                                                   <  2:478081/150‑1 (MQ=255)
                                                                                                  aGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGaaca                                                  <  1:3195228/150‑1 (MQ=255)
                                                                                                     aaTCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAAcc                                               >  2:183508/1‑150 (MQ=255)
                                                                                                      aTCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCt                                              <  1:4480989/150‑1 (MQ=255)
                                                                                                      aTCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCt                                              <  2:759330/150‑1 (MQ=255)
                                                                                                       tCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCtt                                             <  1:191759/150‑1 (MQ=255)
                                                                                                         cGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCt                                           <  2:728964/150‑1 (MQ=255)
                                                                                                          gCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTg                                          <  2:2518002/150‑1 (MQ=255)
                                                                                                           cAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGc                                         >  1:3578171/1‑150 (MQ=255)
                                                                                                           cAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGc                                         >  2:3595665/1‑150 (MQ=255)
                                                                                                              ccTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCgg                                      <  1:3813227/150‑1 (MQ=255)
                                                                                                               cTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGa                                     <  1:2967474/150‑1 (MQ=255)
                                                                                                                     aaaTCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATa                               >  1:3444092/1‑150 (MQ=255)
                                                                                                                     aaaTCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATa                               >  2:3205954/1‑150 (MQ=255)
                                                                                                                       aTCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAAt                             <  2:4610549/150‑1 (MQ=255)
                                                                                                                       aTCCTACGATGGTCAACATGCGGTGGATTATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAAt                             <  2:2835476/150‑1 (MQ=255)
                                                                                                                           tACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAATGCtt                         <  1:1717268/150‑1 (MQ=255)
                                                                                                                              gATGGTCAACATGCGGTGGATTATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAATGCTTGAt                      >  2:2145844/1‑150 (MQ=255)
                                                                                                                              gATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAATGCTTGAt                      >  1:3608266/1‑150 (MQ=255)
                                                                                                                                 ggTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAATGCTTGATGGc                   <  2:3096448/150‑1 (MQ=255)
                                                                                                                                     aaCATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAATGCTTGATGGCGTCg               >  2:549114/1‑150 (MQ=255)
                                                                                                                                     aaCATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAATGCTTGATGGCGTCg               >  1:339632/1‑150 (MQ=255)
                                                                                                                                      aCATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAATGCTTGATGGCGTCGa              <  1:768513/150‑1 (MQ=255)
                                                                                                                                               ggATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAATGCTTGATGGCGTCGATTTGTcaca     <  1:4136711/150‑1 (MQ=255)
                                                                                                                                                 atgatgTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAATGCTTGATGGCGTCGATTTGTCACAgg   <  2:2240528/150‑1 (MQ=255)
                                                                                                                                                 atgatgTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAATGCTTGATGGCGTCGATTTGTCACAgg   <  1:3067690/150‑1 (MQ=255)
                                                                                                                                                 atgatgTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAATGCTTGATGGCGTCGATTTGTCACAgg   <  1:104250/150‑1 (MQ=255)
                                                                                                                                                  tgatgTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAATGCTTGATGGCGTCGATTTGTCACAGGt  >  1:1593514/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAATGCTTGATGGCGTCGATTTGTCACAGGT  >  CP009273/890408‑890703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A