Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,276,723 C→A 17.3% T468T (ACC→ACA)  narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,276,7230CA17.3% 849.2 / 112.9 272T468T (ACC→ACA) narGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (115/110);  new base A (27/20);  total (142/130)
Fisher's exact test for biased strand distribution p-value = 5.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

Only 100 of 278 total aligned reads displayed.

AGCCTGCTGGGTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGATTACCGGCGTTTCTCGCAGCCA  >  CP009273/1276574‑1276872
                                                                                                                                                     |                                                                                                                                                     
aGCCTGCTGGGTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCAcc                                                                                                                                                       <  1:3441509/150‑1 (MQ=255)
 gCCTGCTGGGTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCg                                                                                                                                                      <  1:537934/150‑1 (MQ=255)
   ctgctgGGTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGcc                                                                                                                                                    >  2:2403047/1‑150 (MQ=255)
      ctgGGTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTg                                                                                                                                                 <  1:1459571/150‑1 (MQ=255)
      ctgGGTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACGTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTg                                                                                                                                                 <  2:3217381/150‑1 (MQ=255)
       tgGGTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTgg                                                                                                                                                >  1:2440709/1‑150 (MQ=255)
       tgGGTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACAGCCCTgg                                                                                                                                                >  2:4293310/1‑150 (MQ=255)
        gggTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGt                                                                                                                                               <  1:1095558/150‑1 (MQ=255)
         ggTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTg                                                                                                                                              <  2:3264342/150‑1 (MQ=255)
           tAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACAGCCCTGGTGac                                                                                                                                            <  2:3831845/150‑1 (MQ=255)
                aGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACAGCCCTGGTGACCACCg                                                                                                                                       >  1:4523903/1‑150 (MQ=255)
                   aTGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGttt                                                                                                                                    <  2:1754576/150‑1 (MQ=255)
                         tCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATc                                                                                                                              <  2:1845976/150‑1 (MQ=255)
                         tCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATc                                                                                                                              <  2:4383067/150‑1 (MQ=255)
                           gCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTg                                                                                                                            >  1:61433/1‑150 (MQ=255)
                           gCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTg                                                                                                                            <  1:1430324/150‑1 (MQ=255)
                            cTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGa                                                                                                                           >  1:920143/1‑150 (MQ=255)
                             tGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGAc                                                                                                                          >  2:1970410/1‑150 (MQ=255)
                             tGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGAc                                                                                                                          <  2:95523/150‑1 (MQ=255)
                                ggTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCt                                                                                                                       >  1:712854/1‑150 (MQ=255)
                                  tAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTgg                                                                                                                     <  1:842947/150‑1 (MQ=255)
                                     gCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCaa                                                                                                                  <  1:1571934/150‑1 (MQ=255)
                                      cTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCaaa                                                                                                                 <  1:2307703/150‑1 (MQ=255)
                                      cTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCaaa                                                                                                                 >  2:4026105/1‑150 (MQ=255)
                                        tCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACt                                                                                                               >  1:3574428/1‑150 (MQ=255)
                                        tCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACAGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACt                                                                                                               <  1:2302141/150‑1 (MQ=255)
                                              aCTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTc                                                                                                         >  1:1726730/1‑150 (MQ=255)
                                              aCTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACAGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTc                                                                                                         <  2:1613738/150‑1 (MQ=255)
                                               cTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACAGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCt                                                                                                        <  1:4615225/150‑1 (MQ=255)
                                                tttGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACAGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTg                                                                                                       >  2:2988583/1‑150 (MQ=255)
                                                  tggtggCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGa                                                                                                     <  2:2721621/150‑1 (MQ=255)
                                                   ggtggCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGaa                                                                                                    <  2:2762456/150‑1 (MQ=255)
                                                    gtggCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAAc                                                                                                   <  1:3005132/150‑1 (MQ=255)
                                                         gACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGc                                                                                              >  1:2516939/1‑150 (MQ=255)
                                                         gACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGc                                                                                              <  1:1880326/150‑1 (MQ=255)
                                                           cGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCt                                                                                            >  1:4416722/1‑150 (MQ=255)
                                                            ggCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTg                                                                                           >  1:463602/1‑150 (MQ=255)
                                                            ggCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTg                                                                                           <  2:4244426/150‑1 (MQ=255)
                                                             gCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGa                                                                                          >  1:1383625/1‑150 (MQ=255)
                                                              cACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGaa                                                                                         >  2:4598315/1‑150 (MQ=255)
                                                                 ggAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAacga                                                                                      >  2:4456878/1‑150 (MQ=255)
                                                                 ggAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAAAGTGGCCTGAacga                                                                                      >  2:2255414/1‑150 (MQ=255)
                                                                  gAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAacgac                                                                                     <  1:3720295/150‑1 (MQ=255)
                                                                  gAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAacgac                                                                                     <  2:3692144/150‑1 (MQ=255)
                                                                   aaCACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAacgacg                                                                                    >  2:3909106/1‑150 (MQ=255)
                                                                       cTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTaa                                                                                <  1:3130018/150‑1 (MQ=255)
                                                                         tCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACt                                                                              <  1:2881467/150‑1 (MQ=255)
                                                                          caacaaAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACtg                                                                             <  1:3381391/150‑1 (MQ=255)
                                                                              aaaGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCa                                                                         >  2:1177731/1‑150 (MQ=255)
                                                                               aaGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCaa                                                                        <  1:4258900/150‑1 (MQ=255)
                                                                                aGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAAc                                                                       >  2:2658468/1‑150 (MQ=255)
                                                                                aGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAAc                                                                       <  1:1838106/150‑1 (MQ=255)
                                                                                     aaCTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCt                                                                  <  2:355794/150‑1 (MQ=255)
                                                                                     aaCTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACAGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCt                                                                  >  1:3265778/1‑150 (MQ=255)
                                                                                       cTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTAt                                                                <  2:3453385/150‑1 (MQ=255)
                                                                                        tGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATg                                                               >  2:4100867/1‑150 (MQ=255)
                                                                                        tGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATg                                                               >  2:3128197/1‑150 (MQ=255)
                                                                                        tGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATg                                                               >  2:630755/1‑150 (MQ=255)
                                                                                         ggAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGa                                                              >  1:1013258/1‑150 (MQ=255)
                                                                                         ggAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACAGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGa                                                              <  2:1245752/150‑1 (MQ=255)
                                                                                          gAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACAGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGAc                                                             >  2:1528193/1‑150 (MQ=255)
                                                                                                gTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACAGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGAtgtg                                                       <  1:3225374/150‑1 (MQ=255)
                                                                                                      ctgcACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCt                                                 <  2:74587/150‑1 (MQ=255)
                                                                                                       tgcACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCtt                                                <  1:3352986/150‑1 (MQ=255)
                                                                                                        gcACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTta                                               <  2:2738457/150‑1 (MQ=255)
                                                                                                        gcACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTta                                               >  1:2646860/1‑150 (MQ=255)
                                                                                                         cacaAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTtat                                              <  1:2351186/150‑1 (MQ=255)
                                                                                                          acaAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACAGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTtata                                             >  1:3799409/1‑150 (MQ=255)
                                                                                                           caAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATAc                                            >  1:2733918/1‑150 (MQ=255)
                                                                                                               cTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCg                                        <  2:4149880/150‑1 (MQ=255)
                                                                                                                tGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACAGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCgg                                       >  2:1716620/1‑150 (MQ=255)
                                                                                                                 gCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGc                                      >  1:1985010/1‑150 (MQ=255)
                                                                                                                  ccGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGTTCTGACGCTGGCAAACTACGGTCTGGAACGTGGCGTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGcc                                     <  2:4076628/150‑1 (MQ=255)
                                                                                                                    ggTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTg                                   >  2:2091171/1‑150 (MQ=255)
                                                                                                                      tGAAACGCCTGCAACTGGATGATGGCAGCAACGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTggg                                 >  1:3925812/1‑150 (MQ=255)
                                                                                                                       gAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGc                                <  1:39005/150‑1 (MQ=255)
                                                                                                                       gAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGc                                >  1:2062623/1‑150 (MQ=255)
                                                                                                                        aaaCGCCTGCAACTGGCTGATGTCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGcc                               <  2:8376/150‑1 (MQ=255)
                                                                                                                        aaaCGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGcc                               >  1:4436793/1‑150 (MQ=255)
                                                                                                                        aaaCGCCTGCAACGGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGcc                               <  2:1390723/150‑1 (MQ=255)
                                                                                                                            gCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGc                           <  1:2090336/150‑1 (MQ=255)
                                                                                                                            gCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGc                           <  1:2032853/150‑1 (MQ=255)
                                                                                                                             ccTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCa                          <  1:1885523/150‑1 (MQ=255)
                                                                                                                                gCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGAt                       >  1:969706/1‑150 (MQ=255)
                                                                                                                                 cAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGAtt                      <  2:557730/150‑1 (MQ=255)
                                                                                                                                  aaCTGGCTGATGGCAGCACAGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGATTa                     >  1:3238201/1‑150 (MQ=255)
                                                                                                                                    cTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGATTAcc                   <  1:620570/150‑1 (MQ=255)
                                                                                                                                    cTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGATTAcc                   <  2:1529979/150‑1 (MQ=255)
                                                                                                                                    cTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGATTAcc                   <  2:3581700/150‑1 (MQ=255)
                                                                                                                                    cTGGCTGATGGCAGCACAGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGATTAcc                   <  1:4485785/150‑1 (MQ=255)
                                                                                                                                          gATGGCATCACCGCCCTCGTGACCACCGTTTATGATATGACACTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCACCTAAGACTAAGTGAAAGCTAAAAACCCGGCCTGGGCCGAGCAGAAAACCGACGtt             >  1:2146560/1‑150 (MQ=255)
                                                                                                                                             ggCAGCACAGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGATTACCGGCGTTtct          <  1:2784434/150‑1 (MQ=255)
                                                                                                                                             ggCAGCACAGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGATTACCGGCGTTtct          <  1:1005989/150‑1 (MQ=255)
                                                                                                                                              gcagcaCCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGATTACCGGCGTTtctc         <  1:2440487/150‑1 (MQ=255)
                                                                                                                                               cagcaCCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGATTACCGGCGTTTCTCg        >  1:3655077/1‑150 (MQ=255)
                                                                                                                                                agcaCCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGATTACCGGCGTTTCTCGc       >  2:3044907/1‑150 (MQ=255)
                                                                                                                                                agcaCCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGATTACCGGCGTTTCTCGc       >  1:2676831/1‑150 (MQ=255)
                                                                                                                                                    ccGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGATTACCGGCGTTTCTCGCAGcc   <  1:4167147/150‑1 (MQ=255)
                                                                                                                                                    ccGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGATTACCGGCGTTTCTCGCAGcc   <  1:4026105/150‑1 (MQ=255)
                                                                                                                                                     cGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGATTACCGGCGTTTCTCGCAGCCa  <  1:2545195/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGCCTGCTGGGTAGCCAGGATGAGATCGCTGAGGTAGGCTTCCCGTACTTTGGTGGCGACGGCACGGAACACTTCAACAAAGTGGAACTGGAAAACGTGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGATTACCGGCGTTTCTCGCAGCCA  >  CP009273/1276574‑1276872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A