Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,445,781 G→T 22.2% intergenic (‑175/+73) tynA ← / ← paaZ tyramine oxidase, copper‑requiring/fused oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,445,7810GT22.2% 660.1 / 136.3 251intergenic (‑175/+73)tynA/paaZtyramine oxidase, copper‑requiring/fused oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (89/106);  new base T (29/27);  total (118/133)
Fisher's exact test for biased strand distribution p-value = 4.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 258 total aligned reads displayed.

TGTTAGCAACCGCGCTCTGTGGGCGGTTTAGTCAGGTTCACATTATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGTCAGAATTGAATACAGCGCCACCGGGGTTTGATGCTGAT  >  CP009273/1445634‑1445930
                                                                                                                                                   |                                                                                                                                                     
tGTTAGCAACCGCGCTCTGTGGGCGGTTTAGTCAGGTTCACATTATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCa                                                                                                                                                     >  2:197278/1‑150 (MQ=255)
tGTTAGCAACCGCGCTCTGTGGGCGGTTTAGTCAGGTTCACATTATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCa                                                                                                                                                     >  1:3627856/1‑150 (MQ=255)
 gTTAGCAACCGCGCTCTGTGGGCGGGTTAGTCAGGTTCACATTATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACAtcattcat                                                                                                                                                    >  1:2031399/1‑150 (MQ=255)
   tAGCAACCGCGCTCTGTGGGCGGTTTAGTCAGGTTCACATTATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATaa                                                                                                                                                  >  2:2852817/1‑150 (MQ=255)
     gCAACCGCGCTCTGTGGGCGGTTTAGTCAGGTTCACATTATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAAtg                                                                                                                                                <  2:896764/150‑1 (MQ=255)
       aaCCGCGCTCTGTGGGCGGTTTAGTCAGGTTCACATTATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATTCATAAtgtg                                                                                                                                              >  2:1840047/1‑150 (MQ=255)
       aaCCGCGCTCTGTGGGCGGTTTAGTCAGGTTCACATTATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAAtgtg                                                                                                                                              >  2:1611750/1‑150 (MQ=255)
       aaCCGCGCTCTGTGGGCGGTTTAGTCAGGTTCACATTATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAAtgtg                                                                                                                                              <  2:4496093/150‑1 (MQ=255)
       aaCCGCGCTCTGTGGGCGGTTTAGTCAGGTTCACATTATCAGTACTGATGCAAAGGGGATTGCCTGCACCAGCCAGGGTGGTTGGCAGGTGGGCCTGCTTGTCATACAGTGGGTGCCCGGCAAAGCGATGTACACGGCATGCATAAtgtg                                                                                                                                              >  2:3314990/1‑150 (MQ=255)
        aCCGCGCTCTGTGGGCGGTTTAGTCAGGTTCACATTATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGa                                                                                                                                             >  2:2923562/1‑150 (MQ=255)
          cgcgCTCTGTGGGCGGTTTAGTCAGGTTCACATTATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACCTCATTCATAATGTGACa                                                                                                                                           >  1:343637/1‑150 (MQ=255)
            cgcTCTGTGGGCGGTTTAGTCAGGTTCACATTATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAAc                                                                                                                                         <  1:1820500/150‑1 (MQ=255)
            cgcTCTGTGGGCGGTTTAGTCAGGTTCACATTATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAAc                                                                                                                                         <  1:3648208/150‑1 (MQ=255)
                       cGGTTTAGTCAGGTTCACATTATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACt                                                                                                                              <  1:593571/150‑1 (MQ=255)
                        ggTTTAGTCAGGTTCACATTATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATTCATAATGTGACAACGTCACAAAACtt                                                                                                                             >  1:1614086/1‑150 (MQ=255)
                            tAGTCAGGTTCACATTATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTg                                                                                                                         >  1:3194035/1‑150 (MQ=255)
                             aGTCAGGTTCACATTATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGa                                                                                                                        <  2:37051/150‑1 (MQ=255)
                             aGTCAGGTTCACATTATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGa                                                                                                                        >  2:939106/1‑150 (MQ=255)
                                cAGGTTCACATTATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATTCATAATGTGACAACGTCACAAAACTTAGTGAAAt                                                                                                                     >  2:4251619/1‑150 (MQ=255)
                                cAGGTTCACATTATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAAt                                                                                                                     >  2:2984302/1‑150 (MQ=255)
                                cAGGTTCACATTATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAAt                                                                                                                     <  1:301303/150‑1 (MQ=255)
                                  ggTTCACATTATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATaa                                                                                                                   <  2:373654/150‑1 (MQ=255)
                                   gTTCACATTATCAGTACTGATGCAAAGTGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATaaa                                                                                                                  >  2:55937/1‑150 (MQ=255)
                                   gTTCACATTATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATaaa                                                                                                                  <  1:817684/150‑1 (MQ=255)
                                    ttaacaTTATCAGTACTGATGCAAAGGGGATTGCCTGCTACTGCCAGGTTGTTTGGCAGGTGTGACAGCTTTTCATACAGTGGATGACCTGAAAATTGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATaaaa                                                                                                                 <  1:1559693/147‑1 (MQ=255)
                                      cacaTTATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAgg                                                                                                               <  2:4000841/150‑1 (MQ=255)
                                         aTTATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCa                                                                                                            >  2:2211685/1‑150 (MQ=255)
                                         aTTATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCa                                                                                                            >  1:2069481/1‑150 (MQ=255)
                                          ttATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCaa                                                                                                           <  1:3797699/150‑1 (MQ=255)
                                           tATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATTCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAAc                                                                                                          <  2:2412456/150‑1 (MQ=255)
                                           tATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAAc                                                                                                          >  2:3303874/1‑150 (MQ=255)
                                            aTCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACt                                                                                                         <  2:1728224/150‑1 (MQ=255)
                                              cAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTAt                                                                                                       <  1:1179525/150‑1 (MQ=255)
                                              cAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTAt                                                                                                       <  1:3823660/150‑1 (MQ=255)
                                               aGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTAtt                                                                                                      <  1:698901/150‑1 (MQ=255)
                                                gTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTc                                                                                                     <  1:1420170/150‑1 (MQ=255)
                                                 tACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCg                                                                                                    >  1:31848/1‑150 (MQ=255)
                                                    tGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCg                                                                                                 <  2:1378126/150‑1 (MQ=255)
                                                     gATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGt                                                                                                <  2:3024129/150‑1 (MQ=255)
                                                       tGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATTCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTg                                                                                              >  1:4230003/1‑150 (MQ=255)
                                                       tGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTg                                                                                              <  1:4106716/150‑1 (MQ=255)
                                                        gCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGc                                                                                             >  1:2214017/1‑150 (MQ=255)
                                                         cAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATTCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGcc                                                                                            >  1:175734/1‑150 (MQ=255)
                                                          aaaGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATTCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGccc                                                                                           >  2:776893/1‑150 (MQ=255)
                                                           aaGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATTCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCt                                                                                          >  1:1601860/1‑150 (MQ=255)
                                                            aGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCtt                                                                                         >  2:2398128/1‑150 (MQ=255)
                                                             ggggATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCttc                                                                                        <  2:1495658/150‑1 (MQ=255)
                                                              gggATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATTATGTGACTACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGACCttca                                                                                       <  2:1517955/150‑1 (MQ=255)
                                                               ggATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCttcat                                                                                      <  2:4455055/150‑1 (MQ=255)
                                                                gATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATACAAGGGCAACTATTCGCCGTTGCCCttcatt                                                                                     <  1:3885884/150‑1 (MQ=255)
                                                                   tGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCAc                                                                                  <  1:2241534/150‑1 (MQ=255)
                                                                     ccTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCg                                                                                <  2:388600/150‑1 (MQ=255)
                                                                     ccTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCg                                                                                >  2:2098322/1‑150 (MQ=255)
                                                                     ccTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCg                                                                                <  2:48720/150‑1 (MQ=255)
                                                                     ccTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCg                                                                                <  2:388108/150‑1 (MQ=255)
                                                                      cTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATTCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGa                                                                               >  1:3905255/1‑150 (MQ=255)
                                                                       tGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATTCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGAt                                                                              <  2:3499050/150‑1 (MQ=255)
                                                                       tGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGAt                                                                              <  1:3927274/150‑1 (MQ=255)
                                                                        gCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGAtt                                                                             <  2:1431699/150‑1 (MQ=255)
                                                                           ccTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAAt                                                                          <  1:1958868/150‑1 (MQ=255)
                                                                            cTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATc                                                                         <  2:2637391/150‑1 (MQ=255)
                                                                            cTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATc                                                                         <  1:2624057/150‑1 (MQ=255)
                                                                             tGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCg                                                                        >  1:752300/1‑150 (MQ=255)
                                                                               ccAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATTCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGAc                                                                      >  1:4005177/1‑150 (MQ=255)
                                                                                   gttgttTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACaaaa                                                                  >  1:3687545/1‑150 (MQ=255)
                                                                                     tgttTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATTCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATc                                                                <  2:17388/150‑1 (MQ=255)
                                                                                     tgttTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATTCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATc                                                                <  1:791381/150‑1 (MQ=255)
                                                                                     tgttTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATc                                                                <  1:3229618/150‑1 (MQ=255)
                                                                                         tGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCg                                                            >  1:999503/1‑150 (MQ=255)
                                                                                             aGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGtgct                                                        <  1:960282/150‑1 (MQ=255)
                                                                                                tgtgCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGcc                                                     <  1:3511814/150‑1 (MQ=255)
                                                                                                 gtgCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATTCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCt                                                    <  1:1018798/150‑1 (MQ=255)
                                                                                                 gtgCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCt                                                    <  1:404480/150‑1 (MQ=255)
                                                                                                  tgCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTg                                                   <  1:476612/150‑1 (MQ=255)
                                                                                                  tgCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTg                                                   >  1:2822492/1‑150 (MQ=255)
                                                                                                      atcTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGcca                                               <  1:3376320/148‑1 (MQ=255)
                                                                                                      aGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGcca                                               >  1:1077774/1‑150 (MQ=255)
                                                                                                      aGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGcca                                               >  1:1220525/1‑150 (MQ=255)
                                                                                                            tCATACAGTGGATGCCCTGAAAATAGATGTACACATCATTCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCg                                         >  2:592177/1‑150 (MQ=255)
                                                                                                             cATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGt                                        <  1:931731/150‑1 (MQ=255)
                                                                                                                aCAGTGGATGCCCTGAAAATAGATGTACACATCATTCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGTCAg                                     <  1:4366688/150‑1 (MQ=255)
                                                                                                                     ggATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGTCAGAATTg                                <  1:2324549/150‑1 (MQ=255)
                                                                                                                          cccTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGTCAGAATTGAATAc                           <  2:1319343/150‑1 (MQ=255)
                                                                                                                           ccTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGTCAGAATTGAATACa                          <  1:1340839/150‑1 (MQ=255)
                                                                                                                             tGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGTCAGAATTGAATACAgc                        >  1:781480/1‑150 (MQ=255)
                                                                                                                              gAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGTCAGAATTGAATACAgcg                       >  2:1472866/1‑150 (MQ=255)
                                                                                                                              gAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGTCAGAATTGAATACAgcg                       >  2:1472621/1‑150 (MQ=255)
                                                                                                                                   tAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGTCAGAATTGAATACAGCGCCAcc                  >  2:110956/1‑150 (MQ=255)
                                                                                                                                    aGATGTACACATCATTCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGTCAGAATTGAATACAGCGCCACCg                 <  2:2797865/150‑1 (MQ=255)
                                                                                                                                     gATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGTCAGAATTGAATACAGCGCCACCgg                >  1:394186/1‑150 (MQ=255)
                                                                                                                                       tGTACACATCATTCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGTCAGAATTGAATACAGCGCCACCgggg              >  2:2514784/1‑150 (MQ=255)
                                                                                                                                       tGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGTCAGAATTGAATACAGCGCCACCgggg              >  2:4132084/1‑150 (MQ=255)
                                                                                                                                       tGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGTCAGAATTGAATACAGCGCCACCgggg              >  2:712933/1‑150 (MQ=255)
                                                                                                                                          acacATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGTCAGAATTGAATACAGCGCCACCGGGGttt           >  1:3922983/1‑150 (MQ=255)
                                                                                                                                          acacATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGTCAGAATTGAATACAGCGCCACCGGGGttt           <  2:1277801/150‑1 (MQ=255)
                                                                                                                                           cacaTCAGGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGTCAGAATTGAATACAGCGCCACCGGGGTTTg          <  2:572015/150‑1 (MQ=255)
                                                                                                                                             catcatGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGTCAGAATTGAATCCAGCGCCACCGGGGTTTGAt        <  2:3163928/150‑1 (MQ=255)
                                                                                                                                               tcatGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGTCAGAATTGAATACAGCGCCACCGGGGTTTGATGc      >  1:3730478/1‑150 (MQ=255)
                                                                                                                                                   gCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGTCAGAATTGAATACAGCGCCACCGGGGTTTGATGCTGAt  >  1:4550466/1‑150 (MQ=255)
                                                                                                                                                   gCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGTCAGAATTGAATACAGCGCCACCGGGGTTTGATGCTGAt  <  2:2919345/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TGTTAGCAACCGCGCTCTGTGGGCGGTTTAGTCAGGTTCACATTATCAGTACTGATGCAAAGGGGATTGCCTGCACCTGCCAGGTTGTTTGGCAGGTGTGCCAGCTTTTCATACAGTGGATGCCCTGAAAATAGATGTACACATCATGCATAATGTGACAACGTCACAAAACTTAGTGAAATAAAAGGGCAACTATTCGCCGTTGCCCTTCATTCACCGATTAATCGACAAAATCACCGTGCTGCCTGGCCACCAGCGTCAGAATTGAATACAGCGCCACCGGGGTTTGATGCTGAT  >  CP009273/1445634‑1445930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A