Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,503,014 G→T 42.6% intergenic (+15/+77) ydcP → / ← yncJ putative peptidase/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,503,0140GT42.6% 367.3 / inf 351intergenic (+15/+77)ydcP/yncJputative peptidase/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (100/101);  new base T (71/78);  total (172/179)
Fisher's exact test for biased strand distribution p-value = 7.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 352 total aligned reads displayed.

TTGCCGCCCATGCGAGATGCACGTCATTGGCAAAATCAAAAATCACATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGTTTGCGCGATTTTTGCCAGTAAAAATGCTCGCGCAAACCTTCCGCCGACTCT  >  CP009273/1502867‑1503163
                                                                                                                                                   |                                                                                                                                                     
ttGCCGCCCATGCGAGATGCACGTCATTGGCAAAATCAAAAATCACATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGt                                                                                                                                                     >  1:4078341/1‑150 (MQ=255)
       ccATGCGAGATGCACGTCATTGGCAAAATCAAAAATCACATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTcac                                                                                                                                              >  1:654659/1‑150 (MQ=255)
        cATGCGAGATGCACGTCATTGGCAAAATCAAAAATCACATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTcaca                                                                                                                                             >  1:1144359/1‑150 (MQ=255)
        cATGCGAGATGCACGTCATTGGCAAAATCAAAAATCACATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTcaca                                                                                                                                             <  1:4508458/150‑1 (MQ=255)
        cATGCGAGATGCACGTCATTGGCAAAATCAAAAATCACATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTcaca                                                                                                                                             >  2:72138/1‑150 (MQ=255)
          tGCGAGATGCACGTCATTGGCAAAATCAAAAATCACATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGTGTAAAACGCCAGTTTTCTGGTTACTCACAAc                                                                                                                                           >  1:167550/1‑150 (MQ=255)
           gCGAGATGCACGTCATTGGCAAAATCAAAAATCACATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACt                                                                                                                                          <  1:4418303/150‑1 (MQ=255)
            cGAGATGCACGTCATTGGCAAAATCAAAAATCACATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACtt                                                                                                                                         <  2:4461765/150‑1 (MQ=255)
             gagaTGCACGTCATTGGCAAAATCAAAAATCACATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTa                                                                                                                                        >  2:2749425/1‑150 (MQ=255)
             gagaTGCACGTCATTGGCAAAATCAAAAATCACATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTa                                                                                                                                        >  2:4177838/1‑150 (MQ=255)
              agaTGCACGTCATTGGCAAAATCAAAAATCACATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTAt                                                                                                                                       >  2:3634225/1‑150 (MQ=255)
               gaTGCACGTCATTGGCAAAATCAAAAATCACATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTAtt                                                                                                                                      <  1:4295266/150‑1 (MQ=255)
                   cACGTCATTGGCAAAATCAAAAATCACATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAAt                                                                                                                                  >  1:2705127/1‑150 (MQ=255)
                    aCGTCATTGGCAAAATCAAAAATCACATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTATTGAATc                                                                                                                                 <  2:2623750/150‑1 (MQ=255)
                     cGTCATTGGCAAAATCAAAAATCACATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCt                                                                                                                                <  1:2343237/150‑1 (MQ=255)
                      gTCATTGGCAAAATCAAAAATCACATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTATTGAATCTg                                                                                                                               >  1:1601313/1‑150 (MQ=255)
                      gTCATTGGCAAAATCAAAAATCACATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTATTGAATCTg                                                                                                                               >  1:3293480/1‑150 (MQ=255)
                        cATTGGCAAAATCAAAAATCACATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTATTGAATCTGCa                                                                                                                             >  2:2392751/1‑150 (MQ=255)
                        cATTGGCAAAATCAAAAATCACATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTATTGAATCTGCa                                                                                                                             >  1:3654103/1‑150 (MQ=255)
                         aTTGGAAAAATCAAAAATCAAATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCTTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCAt                                                                                                                            <  1:539412/150‑1 (MQ=255)
                           tGGCAAAATCAAAAATCACATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGa                                                                                                                          >  2:3811765/1‑150 (MQ=255)
                             gCAAAATCAAAAATCACATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTATTGAATCTGCATGata                                                                                                                        <  1:3330166/150‑1 (MQ=255)
                              cAAAATCAAAAATCACATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTATTGAATCTGCATGatat                                                                                                                       <  1:1084842/150‑1 (MQ=255)
                              cAAAATCAAAAATCACATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGatat                                                                                                                       >  2:3504615/1‑150 (MQ=255)
                               aaaatcaaaaatcaCATACTGAAAATGCCGTTACCGGTATGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACAGTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTCTTGAATCTGCATGATAtt                                                                                                                      <  2:1625/150‑1 (MQ=255)
                                 aatcaaaaatcaCATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATtgc                                                                                                                    <  1:1755808/150‑1 (MQ=255)
                                  atcaaaaatcaCATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATtgcc                                                                                                                   >  1:1999150/1‑150 (MQ=255)
                                  atcaaaaatcaCATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATtgcc                                                                                                                   >  1:774780/1‑150 (MQ=255)
                                   tcaaaaatcaCATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTATTGAATCTGCATGATATtgcct                                                                                                                  <  1:3026236/150‑1 (MQ=255)
                                    caaaaatcaCATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTATTGAATCTGCATGATATtgcctg                                                                                                                 <  2:1811490/150‑1 (MQ=255)
                                        aatcaCATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCgg                                                                                                             >  2:3368451/1‑150 (MQ=255)
                                         atcaCATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCggg                                                                                                            <  2:638349/150‑1 (MQ=255)
                                           cacaTACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTa                                                                                                          <  2:2668791/150‑1 (MQ=255)
                                            acaTACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTaa                                                                                                         >  2:3218589/1‑150 (MQ=255)
                                                 cTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGt                                                                                                    <  1:2135250/150‑1 (MQ=255)
                                                  tGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGtt                                                                                                   <  2:4446130/150‑1 (MQ=255)
                                                   gAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTa                                                                                                  >  2:3951764/1‑150 (MQ=255)
                                                     aaaTGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTAcg                                                                                                >  1:3809234/1‑150 (MQ=255)
                                                            gTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATc                                                                                         >  2:2148818/1‑150 (MQ=255)
                                                              tACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCg                                                                                       >  2:389189/1‑150 (MQ=255)
                                                                  gggAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCAt                                                                                   <  2:1749193/150‑1 (MQ=255)
                                                                  gggAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCAt                                                                                   <  1:140767/150‑1 (MQ=255)
                                                                        cGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATg                                                                             >  2:4578680/1‑150 (MQ=255)
                                                                        cGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATg                                                                             <  1:1295016/150‑1 (MQ=255)
                                                                           aGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACt                                                                          >  2:4552108/1‑150 (MQ=255)
                                                                             tGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCa                                                                        <  2:1340589/150‑1 (MQ=255)
                                                                             tGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCa                                                                        >  2:2279491/1‑150 (MQ=255)
                                                                               gCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAAc                                                                      <  2:3793767/150‑1 (MQ=255)
                                                                                cATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACg                                                                     >  1:1201531/1‑150 (MQ=255)
                                                                                cATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACg                                                                     >  1:4225865/1‑150 (MQ=255)
                                                                                  tCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGtt                                                                   <  2:1160955/150‑1 (MQ=255)
                                                                                  tCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGtt                                                                   <  1:224081/150‑1 (MQ=255)
                                                                                   ccGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTg                                                                  <  2:891996/150‑1 (MQ=255)
                                                                                     gTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTc                                                                <  1:2750026/150‑1 (MQ=255)
                                                                                     gTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTc                                                                <  2:806406/150‑1 (MQ=255)
                                                                                      tAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTcc                                                               >  1:3126102/1‑150 (MQ=255)
                                                                                       aaGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTccc                                                              >  1:4059883/1‑150 (MQ=255)
                                                                                       aaGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTccc                                                              <  1:1743518/150‑1 (MQ=255)
                                                                                          tCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCt                                                           <  2:3050311/150‑1 (MQ=255)
                                                                                           ccGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCtt                                                          <  2:428692/150‑1 (MQ=255)
                                                                                           ccGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCtt                                                          <  1:2632592/150‑1 (MQ=255)
                                                                                           ccGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCtt                                                          <  1:1892939/150‑1 (MQ=255)
                                                                                           ccGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCtt                                                          <  2:1182581/150‑1 (MQ=255)
                                                                                           ccGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCtt                                                          <  2:1872404/150‑1 (MQ=255)
                                                                                              gATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTg                                                       <  2:1477767/150‑1 (MQ=255)
                                                                                              gATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTg                                                       >  2:1900788/1‑150 (MQ=255)
                                                                                               aTGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTgg                                                      >  1:3439500/1‑150 (MQ=255)
                                                                                               aTGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTgg                                                      <  2:535482/150‑1 (MQ=255)
                                                                                                  aGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGttt                                                   >  1:98386/1‑150 (MQ=255)
                                                                                                  aGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGttt                                                   <  2:2880251/150‑1 (MQ=255)
                                                                                                      gctgAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGTTTgcgc                                               >  1:3575243/1‑150 (MQ=255)
                                                                                                      gctgAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGTTTgcgc                                               >  2:2463242/1‑150 (MQ=255)
                                                                                                      gctgAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGTTTgcgc                                               <  2:2083266/150‑1 (MQ=255)
                                                                                                       ctgAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGTTTGcgcg                                              <  2:1419790/150‑1 (MQ=255)
                                                                                                         gAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGTTTGCGCGAt                                            >  2:833226/1‑150 (MQ=255)
                                                                                                           aaaCATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGTTTGCGCGAttt                                          >  2:3384892/1‑150 (MQ=255)
                                                                                                            aaCATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGTTTGCGCGAtttt                                         <  1:87646/150‑1 (MQ=255)
                                                                                                             aCATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGTTTGCGCGAttttt                                        <  2:2948943/150‑1 (MQ=255)
                                                                                                             aCATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGTTTGCGCGAttttt                                        <  1:2497921/150‑1 (MQ=255)
                                                                                                              cATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGTTTGCGCGATTTTTg                                       >  2:1562712/1‑150 (MQ=255)
                                                                                                              cATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGTTTGCGCGATTTTTg                                       <  1:2928805/150‑1 (MQ=255)
                                                                                                               aTTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGTTTGCGCGATTTTTGc                                      >  2:2392836/1‑150 (MQ=255)
                                                                                                                   ccGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGTTTGCGCGATTTTTGCCAGt                                  >  1:2852718/1‑150 (MQ=255)
                                                                                                                     gAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGTTTGCGCGATTTTTGCCAGTaa                                >  2:3792316/1‑150 (MQ=255)
                                                                                                                      aaGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGTTTGCGCGATTTTTGCCAGTaaa                               >  1:3861952/1‑150 (MQ=255)
                                                                                                                        gCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGTTTGCGCGATTTTTGCCAGTaaaaa                             <  1:2444765/150‑1 (MQ=255)
                                                                                                                         cGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGTTTGCGCGATTTTTGCCAGTAAAAAt                            >  1:2656296/1‑150 (MQ=255)
                                                                                                                           aaaaGGGTAAAACGCCAGTTTTCTTGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGTTTGCGCGATTTTTGCCAGTAAAAATGc                          >  2:3679633/1‑150 (MQ=255)
                                                                                                                           aaaaGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGTTTGCGCGATTTTTGCCAGTAAAAATGc                          >  1:2407138/1‑150 (MQ=255)
                                                                                                                            aaaGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGTTTGCGCGATTTTTGCCAGTAAAAATGCt                         >  1:4615423/1‑150 (MQ=255)
                                                                                                                            aaaGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGTTTGCGCGATTTTTGCCAGTAAAAATGCt                         >  2:4612887/1‑150 (MQ=255)
                                                                                                                             aaGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGTTTGCGCGATTTTTGCCAGTAAAAATGCTc                        >  1:1020079/1‑150 (MQ=255)
                                                                                                                              aGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGTTTGCGCGATTTTTGCCAGTAAAAATGCTcg                       <  2:4011724/150‑1 (MQ=255)
                                                                                                                              aGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGTTTGCGCGATTTTTGCCAGTAAAAATGCTcg                       >  2:3565531/1‑150 (MQ=255)
                                                                                                                                  tAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGTTTGCGCGATTTTTGCCAGTAAAAATGCTCGCGCa                   <  2:2912642/150‑1 (MQ=255)
                                                                                                                                          cAGTTTTCTTGTTACTCACAACTTATTGAATCTGCATGTTATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGTTTGCGCGATTTTTGCCAGTCAAAATGCTCGCGCAAACCTTcc           >  2:3173869/1‑150 (MQ=255)
                                                                                                                                           aGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGTTTGCGCGATTTTTGCCAGTAAAAATGCTCGCGCAAACCTTccg          >  2:2031438/1‑150 (MQ=255)
                                                                                                                                            gTTTTCTTGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGTTTGCGCGATTTTTGCCAGTAAAAATGCTCGCGCAAACCTTccgc         >  1:370794/1‑150 (MQ=255)
                                                                                                                                              tttCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGTTTGCGCGATTTTTGCCAGTAAAAATGCTCGCGCAAACCTTccgccg       >  2:3132315/1‑150 (MQ=255)
                                                                                                                                                  tGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGTTTGCGCGATTTTTGCCAGTAAAAATGCTCGCGCAAACCTTCCGCCGActc   >  1:3527343/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TTGCCGCCCATGCGAGATGCACGTCATTGGCAAAATCAAAAATCACATACTGAAAATGCCGTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCGGCATCAAATGACTCAACGTTGTCCCGCTTCTGGTTTGCGCGATTTTTGCCAGTAAAAATGCTCGCGCAAACCTTCCGCCGACTCT  >  CP009273/1502867‑1503163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A