Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,572,192 G→T 39.5% L470I (CTT→ATT)  yddA ← putative multidrug transporter subunit of ABC superfamily, membrane component/ATP‑binding component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,572,1920GT39.5% 370.4 / 305.3 296L470I (CTT→ATT) yddAputative multidrug transporter subunit of ABC superfamily, membrane component/ATP‑binding component
Reads supporting (aligned to +/- strand):  ref base G (93/85);  new base T (56/62);  total (149/147)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 297 total aligned reads displayed.

TTCCTCAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCTTTA  >  CP009273/1572043‑1572340
                                                                                                                                                     |                                                                                                                                                    
  ccTCAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGAc                                                                                                                                                    <  1:4303873/150‑1 (MQ=255)
    tCAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCa                                                                                                                                                  >  1:622485/1‑150 (MQ=255)
     cAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAATACCaa                                                                                                                                                 >  2:399083/1‑150 (MQ=255)
     cAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCaa                                                                                                                                                 >  2:1916741/1‑150 (MQ=255)
       aGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAAcc                                                                                                                                               >  1:1078161/1‑150 (MQ=255)
         aTGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAATACCAACCTg                                                                                                                                             <  1:4488161/150‑1 (MQ=255)
         aTGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAATACCAACCTg                                                                                                                                             <  1:884242/150‑1 (MQ=255)
          tGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGa                                                                                                                                            >  2:2719250/1‑150 (MQ=255)
                   gTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACt                                                                                                                                   >  2:2783704/1‑150 (MQ=255)
                     ttCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTc                                                                                                                                 <  2:4206897/150‑1 (MQ=255)
                       cGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGc                                                                                                                               >  1:2780913/1‑150 (MQ=255)
                        gTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCt                                                                                                                              <  2:1450513/150‑1 (MQ=255)
                          cAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCa                                                                                                                            >  1:982870/1‑150 (MQ=255)
                             gAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACg                                                                                                                         <  1:17402/150‑1 (MQ=255)
                             gAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACg                                                                                                                         >  1:538512/1‑150 (MQ=255)
                             gAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACg                                                                                                                         >  2:2116903/1‑150 (MQ=255)
                              aaaTATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGa                                                                                                                        >  2:466990/1‑150 (MQ=255)
                               aaTATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGAt                                                                                                                       <  1:399083/150‑1 (MQ=255)
                               aaTATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGAt                                                                                                                       >  2:536450/1‑150 (MQ=255)
                               aaTATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGAt                                                                                                                       <  2:2263910/150‑1 (MQ=255)
                                 tatCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGAttt                                                                                                                     <  1:3768259/150‑1 (MQ=255)
                                  atCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGGGGTCATGAATACGCGCAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGATTTa                                                                                                                    >  2:1132611/1‑150 (MQ=255)
                                     cATTTCGGACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGATTTATCg                                                                                                                 >  1:2200286/1‑150 (MQ=255)
                                     cATTTCGGACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCg                                                                                                                 >  2:2275066/1‑150 (MQ=255)
                                      aTTTCGGACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGt                                                                                                                <  1:4196307/150‑1 (MQ=255)
                                        ttCGGACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCt                                                                                                              >  2:2904944/1‑150 (MQ=255)
                                          cGGACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTAc                                                                                                            <  2:1148653/150‑1 (MQ=255)
                                           ggACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACg                                                                                                           >  1:2447073/1‑150 (MQ=255)
                                           ggACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACg                                                                                                           <  2:3490045/150‑1 (MQ=255)
                                           ggACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGGGGTCATGAATACGCGCAGCCAATTTCCCAATACCAACCAGATGCAGTACTTCGCTCAACGATTTATCGTCTACg                                                                                                           >  2:2974166/1‑150 (MQ=255)
                                                tcgtAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAg                                                                                                      <  2:1790147/150‑1 (MQ=255)
                                                 cgtAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAgg                                                                                                     >  2:622670/1‑150 (MQ=255)
                                                  gtAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAggg                                                                                                    >  2:4185886/1‑150 (MQ=255)
                                                     aaaTTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGaa                                                                                                 >  1:4498345/1‑150 (MQ=255)
                                                     aaaTTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGaa                                                                                                 >  2:4249213/1‑150 (MQ=255)
                                                      aaTTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAg                                                                                                >  2:75743/1‑150 (MQ=255)
                                                       aTTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGt                                                                                               <  1:2990775/150‑1 (MQ=255)
                                                          aaTCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGACGTGCt                                                                                            >  2:2612945/1‑150 (MQ=255)
                                                          aaTCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCt                                                                                            >  1:2101745/1‑150 (MQ=255)
                                                           aTCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCtt                                                                                           <  1:1767807/150‑1 (MQ=255)
                                                           aTCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCtt                                                                                           >  2:3388448/1‑150 (MQ=255)
                                                           aTCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCtt                                                                                           >  1:2600719/1‑150 (MQ=255)
                                                            tCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCttt                                                                                          <  2:587800/150‑1 (MQ=255)
                                                               tGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACa                                                                                       >  1:3576460/1‑150 (MQ=255)
                                                                  cAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAaat                                                                                    <  2:2410950/150‑1 (MQ=255)
                                                                     ggCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAaataat                                                                                 >  1:102868/1‑150 (MQ=255)
                                                                        gATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAAtctc                                                                              >  1:332218/1‑150 (MQ=255)
                                                                        gATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAAtctc                                                                              >  2:1366109/1‑150 (MQ=255)
                                                                           aCGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCttt                                                                           <  2:1994466/150‑1 (MQ=255)
                                                                            cGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTc                                                                          <  2:391118/150‑1 (MQ=255)
                                                                              ttgttTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAg                                                                        <  1:1604089/150‑1 (MQ=255)
                                                                              ttgttTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAg                                                                        <  2:359298/150‑1 (MQ=255)
                                                                              ttgttTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAg                                                                        <  1:3180504/150‑1 (MQ=255)
                                                                              ttgttTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAg                                                                        >  1:1662193/1‑150 (MQ=255)
                                                                                gttTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTa                                                                      <  1:3051099/150‑1 (MQ=255)
                                                                                gttTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTa                                                                      <  1:1086600/150‑1 (MQ=255)
                                                                                   tttCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGc                                                                   >  1:1327843/1‑150 (MQ=255)
                                                                                         cgcTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGttt                                                             >  2:1322576/1‑150 (MQ=255)
                                                                                            tGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGa                                                          <  2:423861/150‑1 (MQ=255)
                                                                                            tGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGa                                                          <  1:1892138/150‑1 (MQ=255)
                                                                                             ggAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGAt                                                         >  2:4580304/1‑150 (MQ=255)
                                                                                              gAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGAtt                                                        <  2:4478927/150‑1 (MQ=255)
                                                                                                aaGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTaa                                                      >  2:1939543/1‑150 (MQ=255)
                                                                                                aaGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTaa                                                      <  2:1980876/150‑1 (MQ=255)
                                                                                                 aGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAAc                                                     <  2:3281247/150‑1 (MQ=255)
                                                                                                 aGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAAc                                                     <  2:3987254/150‑1 (MQ=255)
                                                                                                   aaTATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACgg                                                   >  1:1799423/1‑150 (MQ=255)
                                                                                                   aaTATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACgg                                                   >  2:376230/1‑150 (MQ=255)
                                                                                                    atatCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGgt                                                  >  1:3279703/1‑150 (MQ=255)
                                                                                                          ccccAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTtgt                                            >  2:1563493/1‑150 (MQ=255)
                                                                                                           cccAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTtgtg                                           <  1:1367453/150‑1 (MQ=255)
                                                                                                            ccAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGa                                          >  2:944114/1‑150 (MQ=255)
                                                                                                            ccAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGa                                          <  1:145490/150‑1 (MQ=255)
                                                                                                             cAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGac                                         >  1:483143/1‑150 (MQ=255)
                                                                                                              aGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGaca                                        >  1:2742149/1‑150 (MQ=255)
                                                                                                              aGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGaca                                        >  1:2864024/1‑150 (MQ=255)
                                                                                                               gCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGacac                                       <  1:3549822/150‑1 (MQ=255)
                                                                                                                cGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGAcaca                                      >  1:3917106/1‑150 (MQ=255)
                                                                                                                 gATCGTGGTCATGAATACGCGCAGCCAATTTCCCAATACCAACCTTATGCAGTTCTTCGCTCAACGATTTATCGTCTACGGGCAGGGGATGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACAt                                     <  1:200192/150‑1 (MQ=255)
                                                                                                                 gATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGACGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACAt                                     >  2:1035290/1‑150 (MQ=255)
                                                                                                                      tGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCaa                                <  2:302428/150‑1 (MQ=255)
                                                                                                                        gTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACt                              <  2:2344550/150‑1 (MQ=255)
                                                                                                                        gTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACt                              <  1:684851/150‑1 (MQ=255)
                                                                                                                            tGAATACGCGCAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTca                          <  2:3104270/150‑1 (MQ=255)
                                                                                                                            tGAATACGCGCAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTca                          <  1:2544722/150‑1 (MQ=255)
                                                                                                                            tGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTca                          <  2:2958188/150‑1 (MQ=255)
                                                                                                                               aTACGCGCAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTcagca                       >  2:2026041/1‑150 (MQ=255)
                                                                                                                               aTACGCGCAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTcagca                       >  2:4351014/1‑150 (MQ=255)
                                                                                                                               aTACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTcagca                       <  2:1862475/150‑1 (MQ=255)
                                                                                                                                 aCGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAgg                     <  2:3677103/150‑1 (MQ=255)
                                                                                                                                  cgcgCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGga                    >  1:3697979/1‑150 (MQ=255)
                                                                                                                                    cgcAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGgaga                  <  1:4464443/150‑1 (MQ=255)
                                                                                                                                     gcAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGagaa                 >  1:1168322/1‑150 (MQ=255)
                                                                                                                                     gcAGCCAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGagaa                 >  1:4432099/1‑150 (MQ=255)
                                                                                                                                          cAATTTCCCAATACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAat            <  1:3955495/150‑1 (MQ=255)
                                                                                                                                          cAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAat            >  1:4154043/1‑150 (MQ=255)
                                                                                                                                                  cAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCtt    <  1:4221685/150‑1 (MQ=255)
                                                                                                                                                   aaGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCttt   <  2:1608787/150‑1 (MQ=255)
                                                                                                                                                    aGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTTACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCTTTa  <  1:294109/150‑1 (MQ=255)
                                                                                                                                                    aGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTCACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCTTTa  <  1:3414001/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTCCTCAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTGCTTTACAAATAATCTCTTTCAGTAAGCCGGTTTTGATTAACGGTGTTTGTGACACATACCAACTGTCAGCAGGAGAAGAAATATCACCTTTA  >  CP009273/1572043‑1572340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A