Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,321,041 G→T 15.9% F194L (TTC→TTA)  yfaP ← DUF2135 family protein, putative host defense protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,321,0410GT15.9% 820.8 / 95.3 258F194L (TTC→TTA) yfaPDUF2135 family protein, putative host defense protein
Reads supporting (aligned to +/- strand):  ref base G (124/93);  new base T (23/18);  total (147/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 263 total aligned reads displayed.

ATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAG  >  CP009273/2320892‑2321190
                                                                                                                                                     |                                                                                                                                                     
aTCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCg                                                                                                                                                       <  1:4103465/150‑1 (MQ=255)
aTCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCg                                                                                                                                                       >  1:2821844/1‑150 (MQ=255)
  cGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGaa                                                                                                                                                     >  2:146535/1‑150 (MQ=255)
   ggTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCGTGGCGaaa                                                                                                                                                    >  1:1580181/1‑150 (MQ=255)
   ggTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCTaaa                                                                                                                                                    >  2:1126958/1‑150 (MQ=255)
       cAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAAtct                                                                                                                                                <  1:2124808/150‑1 (MQ=255)
        aaTAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAAtctc                                                                                                                                               >  2:787935/1‑150 (MQ=255)
          tAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCgg                                                                                                                                             >  2:3151635/1‑150 (MQ=255)
          tAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCgg                                                                                                                                             <  1:1372897/150‑1 (MQ=255)
          tAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCgg                                                                                                                                             >  1:1793798/1‑150 (MQ=255)
              tGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCTAAAATCTCGGGTcc                                                                                                                                         <  1:4547138/150‑1 (MQ=255)
                     tGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTAcccc                                                                                                                                  >  2:109653/1‑150 (MQ=255)
                      gTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCcg                                                                                                                                 >  2:786913/1‑150 (MQ=255)
                          ctcATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCTAAAATCTCGGGTCCGTACCCcgtcgt                                                                                                                             <  1:2856531/150‑1 (MQ=255)
                          ctcATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCcgtcgt                                                                                                                             >  2:488371/1‑150 (MQ=255)
                           tcATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTg                                                                                                                            <  2:3766111/150‑1 (MQ=255)
                                  ccGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCa                                                                                                                     >  1:785586/1‑150 (MQ=255)
                                   cGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCat                                                                                                                    >  1:591786/1‑150 (MQ=255)
                                   cGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCat                                                                                                                    >  2:435819/1‑150 (MQ=255)
                                    gATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCTAAAATCTCGGGTCCGTACCCCGTCGTGACATCCata                                                                                                                   >  2:61515/1‑150 (MQ=255)
                                    gATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCata                                                                                                                   >  1:2240082/1‑150 (MQ=255)
                                    gATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCata                                                                                                                   >  1:3421589/1‑150 (MQ=255)
                                    gATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCata                                                                                                                   >  1:2883861/1‑150 (MQ=255)
                                      tCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATc                                                                                                                 >  2:449425/1‑150 (MQ=255)
                                      tCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATc                                                                                                                 >  2:1267389/1‑150 (MQ=255)
                                       ccTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATcc                                                                                                                <  2:1992726/150‑1 (MQ=255)
                                       ccTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATcc                                                                                                                <  2:3269725/150‑1 (MQ=255)
                                       ccTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCAGTGAATTGGCGCGGGCATGGAGAAAATCTCGGGTCAGTACCCCGTCGTGACATCCATATcc                                                                                                                >  1:3025613/1‑150 (MQ=255)
                                        cTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCa                                                                                                               <  2:4292538/150‑1 (MQ=255)
                                        cTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCa                                                                                                               >  2:3714238/1‑150 (MQ=255)
                                        cTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTACGTATCTCAGCTCCCACCATAGTAGTTGATATACAACTGATAGCGCACGTGAATTGTAGCGGGCATGGCGAAAATCTCGTGTCCGTACCCCGACGTGACATCCATATCCa                                                                                                               >  1:430430/1‑150 (MQ=255)
                                         ttCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAg                                                                                                              >  2:2331171/1‑150 (MQ=255)
                                          tCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCCCCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGt                                                                                                             >  2:1628947/1‑150 (MQ=255)
                                          tCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGt                                                                                                             <  2:450417/150‑1 (MQ=255)
                                          tCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGt                                                                                                             >  2:530946/1‑150 (MQ=255)
                                           cATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCTAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTg                                                                                                            <  1:2860929/150‑1 (MQ=255)
                                              cGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGcac                                                                                                         >  1:2424272/1‑150 (MQ=255)
                                                 tGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGcaccac                                                                                                      >  1:1112857/1‑150 (MQ=255)
                                                     cAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCTAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGtt                                                                                                  >  1:788810/1‑150 (MQ=255)
                                                     cAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCTAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGtt                                                                                                  <  2:658122/150‑1 (MQ=255)
                                                        cGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGttttt                                                                                               >  2:139938/1‑150 (MQ=255)
                                                          tCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCa                                                                                             <  1:1441415/150‑1 (MQ=255)
                                                            aGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGc                                                                                           <  1:1773509/150‑1 (MQ=255)
                                                            aGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGc                                                                                           <  2:1570822/150‑1 (MQ=255)
                                                              ttGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCAc                                                                                         <  1:3822771/150‑1 (MQ=255)
                                                               tGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACg                                                                                        >  2:3479435/1‑150 (MQ=255)
                                                               tGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACg                                                                                        >  2:1949963/1‑150 (MQ=255)
                                                                gggCGGTGGTTAAGTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAAGTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTGTTTCAGCACgg                                                                                       >  2:2797531/1‑150 (MQ=255)
                                                                 ggCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGgt                                                                                      >  2:1599695/1‑150 (MQ=255)
                                                                 ggCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGgt                                                                                      <  2:204528/150‑1 (MQ=255)
                                                                   cGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCTAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGgtgt                                                                                    >  1:1239256/1‑150 (MQ=255)
                                                                    ggtggtTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCTAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGtt                                                                                   >  2:1260798/1‑150 (MQ=255)
                                                                          tAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTa                                                                             >  1:395821/1‑150 (MQ=255)
                                                                            aTTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTAcc                                                                           <  1:1596714/150‑1 (MQ=255)
                                                                               ccGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAgg                                                                        <  1:4457249/150‑1 (MQ=255)
                                                                                cGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGc                                                                       >  2:2878906/1‑150 (MQ=255)
                                                                                cGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGc                                                                       >  1:194289/1‑150 (MQ=255)
                                                                                 gTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCAAGAGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCg                                                                      >  1:1354847/1‑150 (MQ=255)
                                                                                  tttCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCgt                                                                     >  2:2230750/1‑150 (MQ=255)
                                                                                   ttCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCTAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCgtg                                                                    >  1:2559318/1‑150 (MQ=255)
                                                                                       cTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCg                                                                <  2:2971904/150‑1 (MQ=255)
                                                                                        tGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCTAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGc                                                               <  2:3897635/150‑1 (MQ=255)
                                                                                         gcgcCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGcc                                                              <  1:1009899/150‑1 (MQ=255)
                                                                                          cgcCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCa                                                             >  1:2376497/1‑150 (MQ=255)
                                                                                            cccACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAACATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATc                                                           <  1:3393170/150‑1 (MQ=255)
                                                                                                  aTAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGt                                                     <  2:2566708/150‑1 (MQ=255)
                                                                                                    agtagtTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTaa                                                   <  2:2132637/150‑1 (MQ=255)
                                                                                                       agtTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTaacaa                                                >  2:1885433/1‑150 (MQ=255)
                                                                                                       agtTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTaacaa                                                >  2:4245402/1‑150 (MQ=255)
                                                                                                           gATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTg                                            >  1:570236/1‑150 (MQ=255)
                                                                                                             tataCACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCTAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGaa                                          >  2:4180807/1‑150 (MQ=255)
                                                                                                              ataCACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAag                                         >  2:3237324/1‑150 (MQ=255)
                                                                                                                acacCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAaggt                                       <  1:3572533/150‑1 (MQ=255)
                                                                                                                 cacCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAaggtc                                      <  1:908768/150‑1 (MQ=255)
                                                                                                                   ccTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAaggtcga                                    <  2:3636300/150‑1 (MQ=255)
                                                                                                                   ccTGATAGCGCCCGTGAATTGGCGAGTGCATGGCTAATATCTCTGGTCCGGACCCAGTCGTGACATCCCTATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAtggtcga                                    <  2:387694/150‑1 (MQ=255)
                                                                                                                     tGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAaggtcgagg                                  >  2:3600092/1‑150 (MQ=255)
                                                                                                                       aTAGCGCCCGTGAATTGGCGCGGGCATGGCTAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAaggtcgaggtc                                >  1:3518021/1‑150 (MQ=255)
                                                                                                                       aTAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAaggtcgaggtc                                <  2:971323/150‑1 (MQ=255)
                                                                                                                        tAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAaggtcgaggtcg                               <  2:420822/150‑1 (MQ=255)
                                                                                                                        tAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAaggtcgaggtcg                               >  1:870659/1‑150 (MQ=255)
                                                                                                                         aGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCgg                              <  2:2668479/150‑1 (MQ=255)
                                                                                                                          gcgcCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATNGGGCGTAACAACGTGAAGGTCGAGGTCGGt                             <  1:4275360/150‑1 (MQ=255)
                                                                                                                           cgcCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTa                            >  1:4337326/1‑150 (MQ=255)
                                                                                                                              ccGTGAATTGGCGCGGGCATGGCTAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTg                         >  2:3880736/1‑150 (MQ=255)
                                                                                                                              ccGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTg                         <  1:4184716/150‑1 (MQ=255)
                                                                                                                               cGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGt                        >  1:2138126/1‑150 (MQ=255)
                                                                                                                                gTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTc                       >  2:3521106/1‑150 (MQ=255)
                                                                                                                                 tGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTcc                      >  1:1173806/1‑150 (MQ=255)
                                                                                                                                 tGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTcc                      <  2:3938632/150‑1 (MQ=255)
                                                                                                                                     ttGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTAt                  <  1:4067582/150‑1 (MQ=255)
                                                                                                                                      tGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATc                 <  2:3198038/150‑1 (MQ=255)
                                                                                                                                       ggCGCGGGCATGGCTAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATcc                <  1:3867264/150‑1 (MQ=255)
                                                                                                                                         cgcgGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCa              >  2:1054276/1‑150 (MQ=255)
                                                                                                                                          gcgGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCAc             <  1:1006552/150‑1 (MQ=255)
                                                                                                                                           cgGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACg            <  1:2688285/150‑1 (MQ=255)
                                                                                                                                           cgGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACg            >  2:1771329/1‑150 (MQ=255)
                                                                                                                                              gCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACgaga         >  1:861715/1‑150 (MQ=255)
                                                                                                                                                 tGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGaa      <  2:3829312/150‑1 (MQ=255)
                                                                                                                                                   gCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAAcc    <  1:729996/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAG  >  CP009273/2320892‑2321190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A