Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,330,502 C→A 92.8% D800Y (GAT→TAT)  gyrA ← DNA gyrase (type II topoisomerase), subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,330,5020CA92.8% 671.9 / 43.8 230D800Y (GAT→TAT) gyrADNA gyrase (type II topoisomerase), subunit A
Reads supporting (aligned to +/- strand):  ref base C (9/8);  new base A (93/120);  total (102/128)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 232 total aligned reads displayed.

ATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAAC  >  CP009273/2330353‑2330648
                                                                                                                                                     |                                                                                                                                                  
aTCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATc                                                                                                                                                    >  1:925289/1‑150 (MQ=255)
aTCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATc                                                                                                                                                    <  2:598794/150‑1 (MQ=255)
 tCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCg                                                                                                                                                   <  1:1525896/150‑1 (MQ=255)
      aTCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCAGAGGTGat                                                                                                                                              >  2:3575004/1‑150 (MQ=255)
       tCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGACACGCGAGTACGTACCAGCGTACCGGCATAGGTGatc                                                                                                                                             <  2:4210934/150‑1 (MQ=255)
       tCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGatc                                                                                                                                             >  2:3822255/1‑150 (MQ=255)
        cTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGatca                                                                                                                                            >  2:1963184/1‑150 (MQ=255)
           ccTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGAtcatca                                                                                                                                         <  1:1807663/150‑1 (MQ=255)
            cTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATcatcat                                                                                                                                        >  1:3260447/1‑150 (MQ=255)
            cTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATcatcat                                                                                                                                        <  1:3913268/150‑1 (MQ=255)
              cGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGa                                                                                                                                      <  2:2693189/150‑1 (MQ=255)
               gTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGAt                                                                                                                                     <  2:261639/150‑1 (MQ=255)
                tCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATc                                                                                                                                    >  2:2564278/1‑150 (MQ=255)
                 cAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACTATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCt                                                                                                                                   <  1:599649/150‑1 (MQ=255)
                    ccGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGACACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGt                                                                                                                                >  2:177198/1‑150 (MQ=255)
                    ccGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGt                                                                                                                                <  2:4085620/150‑1 (MQ=255)
                     cGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTc                                                                                                                               >  1:357672/1‑150 (MQ=255)
                     cGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTc                                                                                                                               >  2:2872273/1‑150 (MQ=255)
                      ggTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCATACCGGCATAGGTGATCATCATGATCTGGTCg                                                                                                                              <  1:4068798/150‑1 (MQ=255)
                       gTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGc                                                                                                                             <  1:1612894/150‑1 (MQ=255)
                          cAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGt                                                                                                                          >  2:4458029/1‑150 (MQ=255)
                              aaCACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATc                                                                                                                      >  1:4130338/1‑150 (MQ=255)
                               acacGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCt                                                                                                                     <  2:208990/150‑1 (MQ=255)
                                cccGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTa                                                                                                                    <  1:3211294/148‑1 (MQ=255)
                                 acGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTAc                                                                                                                   <  2:3289058/150‑1 (MQ=255)
                                   gTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCt                                                                                                                 <  1:3513639/150‑1 (MQ=255)
                                      gCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTa                                                                                                              <  2:4373886/150‑1 (MQ=255)
                                       cAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTAc                                                                                                             <  1:2583401/150‑1 (MQ=255)
                                        aGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTAcc                                                                                                            <  2:3133238/150‑1 (MQ=255)
                                          aCCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACcgc                                                                                                          <  2:3366/150‑1 (MQ=255)
                                              aCTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCca                                                                                                      <  1:1653981/150‑1 (MQ=255)
                                               cTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCcaa                                                                                                     >  1:299994/1‑150 (MQ=255)
                                                tACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCcaac                                                                                                    >  2:1129927/1‑150 (MQ=255)
                                                tACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCcaac                                                                                                    >  2:1270995/1‑150 (MQ=255)
                                                    ttttCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACt                                                                                                >  2:2186621/1‑150 (MQ=255)
                                                     tttCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTa                                                                                               <  1:2365711/150‑1 (MQ=255)
                                                      ttCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTaa                                                                                              >  1:4325953/1‑150 (MQ=255)
                                                       tCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTaaa                                                                                             <  2:3985823/150‑1 (MQ=255)
                                                       tCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTaaa                                                                                             <  1:4248135/150‑1 (MQ=255)
                                                         aTCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAAcc                                                                                           <  2:3090592/150‑1 (MQ=255)
                                                           cTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGt                                                                                         <  1:287534/150‑1 (MQ=255)
                                                              ccGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTAc                                                                                      >  1:759996/1‑150 (MQ=255)
                                                                  aGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTc                                                                                  >  1:1842183/1‑150 (MQ=255)
                                                                      cGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTa                                                                              <  2:512397/150‑1 (MQ=255)
                                                                       ggATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTaa                                                                             >  2:3950591/1‑150 (MQ=255)
                                                                       ggATGAGGATCACGCACTGGGTGTTACGGCCCACGATGCTGATTTCAGAAACGCGAGTACGTACCAGCGTACCGGCATATGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTAACGCGCCAACAACTAAACCGTTACGTTCGGTaa                                                                             >  2:1187868/1‑150 (MQ=255)
                                                                         aTGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAAcc                                                                           >  1:3646364/1‑150 (MQ=255)
                                                                         aTGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAAcc                                                                           <  1:1431989/150‑1 (MQ=255)
                                                                          tGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCt                                                                          <  2:2830698/150‑1 (MQ=255)
                                                                           gAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCtt                                                                         >  2:929640/1‑150 (MQ=255)
                                                                           gAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCtt                                                                         >  2:889197/1‑150 (MQ=255)
                                                                           gAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCtt                                                                         <  1:1697382/150‑1 (MQ=255)
                                                                              gATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGAt                                                                      <  2:2428731/150‑1 (MQ=255)
                                                                              gATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGAt                                                                      <  2:2428488/150‑1 (MQ=255)
                                                                              gATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGAt                                                                      <  2:4493896/150‑1 (MQ=255)
                                                                               aTCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATg                                                                     <  1:2441282/150‑1 (MQ=255)
                                                                                   cGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGgaga                                                                 >  1:2134170/1‑150 (MQ=255)
                                                                                   cGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGgaga                                                                 >  1:2134101/1‑150 (MQ=255)
                                                                                    gCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGAt                                                                <  1:4033577/150‑1 (MQ=255)
                                                                                     cccTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATa                                                               <  1:1145497/150‑1 (MQ=255)
                                                                                      ccTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATaa                                                              >  1:3863474/1‑150 (MQ=255)
                                                                                        tGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAAcc                                                            <  1:424347/150‑1 (MQ=255)
                                                                                          ggTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAAcccc                                                          <  1:166414/150‑1 (MQ=255)
                                                                                             gtTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCttt                                                       <  2:2265171/150‑1 (MQ=255)
                                                                                               tACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTtcg                                                     <  1:3935061/150‑1 (MQ=255)
                                                                                               tACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTtcg                                                     >  2:3343172/1‑150 (MQ=255)
                                                                                                aCGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTtcgt                                                    <  1:154355/150‑1 (MQ=255)
                                                                                                aCGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTtcgt                                                    >  1:3714783/1‑150 (MQ=255)
                                                                                                aCGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTtcgt                                                    <  1:3020042/150‑1 (MQ=255)
                                                                                                 cGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTtcgtc                                                   <  2:2535496/150‑1 (MQ=255)
                                                                                                  ggCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTtcgtcg                                                  >  2:1484551/1‑150 (MQ=255)
                                                                                                  ggCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTtcgtcg                                                  >  2:1243907/1‑150 (MQ=255)
                                                                                                         gATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGa                                           <  2:347413/150‑1 (MQ=255)
                                                                                                           tGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACt                                         >  2:3938674/1‑150 (MQ=255)
                                                                                                              tGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACttgg                                      <  1:1756968/150‑1 (MQ=255)
                                                                                                              tGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACttgg                                      <  1:1430583/150‑1 (MQ=255)
                                                                                                              tGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACttgg                                      <  2:184386/150‑1 (MQ=255)
                                                                                                                  ttCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACttggttgg                                  <  2:3615696/150‑1 (MQ=255)
                                                                                                                    ccGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGt                                >  1:2594872/1‑150 (MQ=255)
                                                                                                                      gAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTAt                              >  1:1938146/1‑150 (MQ=255)
                                                                                                                        aaCGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTc                            <  1:4576972/150‑1 (MQ=255)
                                                                                                                            cgAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGcc                        <  2:3027691/150‑1 (MQ=255)
                                                                                                                             gAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCa                       >  1:2520947/1‑150 (MQ=255)
                                                                                                                             gAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCa                       >  1:4503554/1‑150 (MQ=255)
                                                                                                                             gAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCa                       >  1:261388/1‑150 (MQ=255)
                                                                                                                             gAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCa                       >  1:655078/1‑150 (MQ=255)
                                                                                                                             gAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCa                       >  1:1484248/1‑150 (MQ=255)
                                                                                                                              aGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCAc                      >  2:3128202/1‑150 (MQ=255)
                                                                                                                               gtacgtacCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACt                     <  1:1223198/150‑1 (MQ=255)
                                                                                                                                tacgtacCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTg                    <  1:180422/150‑1 (MQ=255)
                                                                                                                                tacgtacCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTg                    <  2:4579533/150‑1 (MQ=255)
                                                                                                                                  cgtacCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCg                  <  2:1673647/150‑1 (MQ=255)
                                                                                                                                      ccAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTAc              <  2:1338489/150‑1 (MQ=255)
                                                                                                                                          cGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACACCTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGttt          <  2:2489612/150‑1 (MQ=255)
                                                                                                                                          cGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGttt          <  2:4328912/150‑1 (MQ=255)
                                                                                                                                           gTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTa         <  2:2153842/150‑1 (MQ=255)
                                                                                                                                           gTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTa         <  1:822689/150‑1 (MQ=255)
                                                                                                                                            tACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTAc        <  1:2153346/150‑1 (MQ=255)
                                                                                                                                              ccGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCg      <  1:3679956/150‑1 (MQ=255)
                                                                                                                                              ccGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCg      >  2:2328108/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
ATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAAC  >  CP009273/2330353‑2330648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGT  >  CP009273/2330353‑2330651
                                                                                                                                                     |                                                                                                                                                     
ATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATC                                                                                                                                                       >  SRR19973073.927216/1‑150 (MQ=60)
ATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATA                                                                                                                                                       >  SRR19973073.2740769/1‑150 (MQ=60)
 TCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCG                                                                                                                                                      <  SRR19973073.1529034/150‑1 (MQ=60)
 TCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAG                                                                                                                                                      <  SRR19973073.856468/150‑1 (MQ=60)
           CCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCA                                                                                                                                            <  SRR19973073.1811371/150‑1 (MQ=60)
           CCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCA                                                                                                                                            <  SRR19973073.1811395/150‑1 (MQ=60)
            CTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCAT                                                                                                                                           >  SRR19973073.3267049/1‑150 (MQ=60)
            CTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCAT                                                                                                                                           <  SRR19973073.3921045/150‑1 (MQ=60)
                 CAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACTATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCT                                                                                                                                      <  SRR19973073.600932/150‑1 (MQ=60)
                 CAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCT                                                                                                                                      <  SRR19973073.1765219/150‑1 (MQ=60)
                 CAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCT                                                                                                                                      <  SRR19973073.600371/150‑1 (MQ=60)
                     CGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTC                                                                                                                                  >  SRR19973073.358389/1‑150 (MQ=60)
                     CGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTC                                                                                                                                  <  SRR19973073.2962090/150‑1 (MQ=60)
                      GGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCG                                                                                                                                 <  SRR19973073.4076954/150‑1 (MQ=60)
                      GGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCATACCGGCATAGGTGATCATCATGATCTGGTCG                                                                                                                                 <  SRR19973073.4076939/150‑1 (MQ=60)
                       GTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGC                                                                                                                                >  SRR19973073.2225500/1‑150 (MQ=60)
                       GTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGC                                                                                                                                <  SRR19973073.1617951/150‑1 (MQ=60)
                       GTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGC                                                                                                                                <  SRR19973073.1616220/150‑1 (MQ=60)
                          CAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGT                                                                                                                             >  SRR19973073.2160411/1‑150 (MQ=60)
                              AACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATC                                                                                                                         >  SRR19973073.1706132/1‑150 (MQ=60)
                              AACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATC                                                                                                                         >  SRR19973073.4138605/1‑150 (MQ=60)
                               ACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCT                                                                                                                        >  SRR19973073.640675/1‑150 (MQ=60)
                               ACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCT                                                                                                                        >  SRR19973073.640849/1‑150 (MQ=60)
                                CCCGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTA                                                                                                                       <  SRR19973073.3217778/150‑1 (MQ=60)
                                CACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTA                                                                                                                       <  SRR19973073.2654698/150‑1 (MQ=60)
                                 ACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTAC                                                                                                                      <  SRR19973073.3264706/150‑1 (MQ=60)
                                 ACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTAC                                                                                                                      <  SRR19973073.942684/150‑1 (MQ=60)
                                   GTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGACACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCT                                                                                                                    <  SRR19973073.3520697/150‑1 (MQ=60)
                                   GTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCT                                                                                                                    >  SRR19973073.2566583/1‑150 (MQ=60)
                                   GTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCT                                                                                                                    >  SRR19973073.2566590/1‑150 (MQ=60)
                                   GTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCT                                                                                                                    <  SRR19973073.3520664/150‑1 (MQ=60)
                                    TTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTG                                                                                                                   <  SRR19973073.4013028/150‑1 (MQ=60)
                                     TGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTATGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGT                                                                                                                  <  SRR19973073.2446243/150‑1 (MQ=60)
                                       CAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTAC                                                                                                                <  SRR19973073.2588717/150‑1 (MQ=60)
                                          ACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGC                                                                                                             >  SRR19973073.2027611/1‑150 (MQ=60)
                                              ACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCA                                                                                                         <  SRR19973073.1657405/150‑1 (MQ=60)
                                              ACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCA                                                                                                         <  SRR19973073.1657411/150‑1 (MQ=60)
                                              ACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCA                                                                                                         <  SRR19973073.3873237/150‑1 (MQ=60)
                                               CTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAA                                                                                                        >  SRR19973073.300626/1‑150 (MQ=60)
                                                     TTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTA                                                                                                  <  SRR19973073.2370550/150‑1 (MQ=60)
                                                      TTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAA                                                                                                 >  SRR19973073.4334595/1‑150 (MQ=60)
                                                       TCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAA                                                                                                <  SRR19973073.4256486/150‑1 (MQ=60)
                                                       TCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAA                                                                                                <  SRR19973073.4256538/150‑1 (MQ=60)
                                                        CATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAAC                                                                                               >  SRR19973073.2140363/1‑150 (MQ=60)
                                                         ATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACC                                                                                              >  SRR19973073.4186765/1‑150 (MQ=60)
                                                           CTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGT                                                                                            <  SRR19973073.288154/150‑1 (MQ=60)
                                                              CCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTAC                                                                                         >  SRR19973073.761524/1‑150 (MQ=60)
                                                                 CAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTT                                                                                      <  SRR19973073.3588406/150‑1 (MQ=60)
                                                                  AGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTC                                                                                     >  SRR19973073.3598071/1‑150 (MQ=60)
                                                                  AGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTC                                                                                     >  SRR19973073.1845967/1‑150 (MQ=60)
                                                                  AGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTC                                                                                     >  SRR19973073.1845979/1‑150 (MQ=60)
                                                                   GTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCG                                                                                    >  SRR19973073.3225523/1‑150 (MQ=60)
                                                                    TACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGG                                                                                   <  SRR19973073.1960590/150‑1 (MQ=60)
                                                                         ATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACC                                                                              <  SRR19973073.1434990/150‑1 (MQ=60)
                                                                         ATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACC                                                                              >  SRR19973073.3653680/1‑150 (MQ=60)
                                                                         ATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACC                                                                              <  SRR19973073.1435001/150‑1 (MQ=60)
                                                                         ATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACC                                                                              >  SRR19973073.1674423/1‑150 (MQ=60)
                                                                           GAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTT                                                                            <  SRR19973073.1700903/150‑1 (MQ=60)
                                                                           GAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTT                                                                            <  SRR19973073.1490211/150‑1 (MQ=60)
                                                                            AGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTG                                                                           <  SRR19973073.3827169/150‑1 (MQ=60)
                                                                             GGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGA                                                                          <  SRR19973073.1191930/150‑1 (MQ=60)
                                                                               ATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATG                                                                        <  SRR19973073.1365421/150‑1 (MQ=60)
                                                                               ATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATG                                                                        <  SRR19973073.2446339/150‑1 (MQ=60)
                                                                                   CGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGA                                                                    >  SRR19973073.3011969/1‑150 (MQ=60)
                                                                                   CGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGA                                                                    >  SRR19973073.2138573/1‑150 (MQ=60)
                                                                                   CGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGA                                                                    >  SRR19973073.2138590/1‑150 (MQ=60)
                                                                                   CGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGA                                                                    >  SRR19973073.2138642/1‑150 (MQ=60)
                                                                                    GCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGAT                                                                   <  SRR19973073.4041702/150‑1 (MQ=60)
                                                                                    GCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGAT                                                                   <  SRR19973073.4041648/150‑1 (MQ=60)
                                                                                    GCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGAT                                                                   <  SRR19973073.1485693/150‑1 (MQ=60)
                                                                                    GCCCTGGGTGTTACGGACCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGAT                                                                   >  SRR19973073.393477/1‑150 (MQ=60)
                                                                                     CCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATA                                                                  <  SRR19973073.1147814/150‑1 (MQ=60)
                                                                                      CCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAA                                                                 >  SRR19973073.1609443/1‑150 (MQ=60)
                                                                                      CCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAA                                                                 <  SRR19973073.4185669/150‑1 (MQ=60)
                                                                                      CCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAA                                                                 <  SRR19973073.1463448/150‑1 (MQ=60)
                                                                                      CCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAA                                                                 >  SRR19973073.3871124/1‑150 (MQ=60)
                                                                                        TGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACC                                                               <  SRR19973073.425199/150‑1 (MQ=60)
                                                                                         GGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCC                                                              >  SRR19973073.4413708/1‑150 (MQ=60)
                                                                                         GGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCC                                                              >  SRR19973073.4413702/1‑150 (MQ=60)
                                                                                          GGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCC                                                             <  SRR19973073.166808/150‑1 (MQ=60)
                                                                                               TACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCG                                                        <  SRR19973073.3942899/150‑1 (MQ=60)
                                                                                                ACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCTTTACTTTCGGTAACCTTGATGGAGATAACCCCTTTCGT                                                       >  SRR19973073.3722244/1‑150 (MQ=60)
                                                                                                ACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGT                                                       >  SRR19973073.338334/1‑150 (MQ=60)
                                                                                                ACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGT                                                       >  SRR19973073.248852/1‑150 (MQ=60)
                                                                                                ACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGT                                                       <  SRR19973073.154724/150‑1 (MQ=60)
                                                                                                ACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGT                                                       <  SRR19973073.3026156/150‑1 (MQ=60)
                                                                                                      CACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACG                                                 >  SRR19973073.457033/1‑150 (MQ=60)
                                                                                                       ACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGC                                                >  SRR19973073.1850152/1‑150 (MQ=60)
                                                                                                           TGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACT                                            <  SRR19973073.316272/150‑1 (MQ=60)
                                                                                                           TGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACT                                            >  SRR19973073.4007613/1‑150 (MQ=60)
                                                                                                              TGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGG                                         <  SRR19973073.1760609/150‑1 (MQ=60)
                                                                                                              TGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGG                                         <  SRR19973073.1433594/150‑1 (MQ=60)
                                                                                                               GATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGT                                        >  SRR19973073.699781/1‑150 (MQ=60)
                                                                                                                ATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTT                                       >  SRR19973073.3869883/1‑150 (MQ=60)
                                                                                                                ATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTT                                       >  SRR19973073.826249/1‑150 (MQ=60)
                                                                                                                ATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTT                                       >  SRR19973073.1544267/1‑150 (MQ=60)
                                                                                                                    CCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGT                                   >  SRR19973073.2600204/1‑150 (MQ=60)
                                                                                                                    CCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGT                                   >  SRR19973073.2600365/1‑150 (MQ=60)
                                                                                                                    CCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGT                                   >  SRR19973073.2600063/1‑150 (MQ=60)
                                                                                                                      GAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTAT                                 >  SRR19973073.1942108/1‑150 (MQ=60)
                                                                                                                        AACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTC                               <  SRR19973073.814523/150‑1 (MQ=60)
                                                                                                                        AACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTC                               <  SRR19973073.4586123/150‑1 (MQ=60)
                                                                                                                             GAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCA                          >  SRR19973073.261966/1‑150 (MQ=60)
                                                                                                                             GAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCA                          >  SRR19973073.2526154/1‑150 (MQ=60)
                                                                                                                             GAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCA                          >  SRR19973073.4512605/1‑150 (MQ=60)
                                                                                                                             GAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCA                          >  SRR19973073.656455/1‑150 (MQ=60)
                                                                                                                             GAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCA                          >  SRR19973073.1487343/1‑150 (MQ=60)
                                                                                                                               GTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACT                        <  SRR19973073.1225750/150‑1 (MQ=60)
                                                                                                                                TACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTG                       <  SRR19973073.180850/150‑1 (MQ=60)
                                                                                                                                TACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTG                       <  SRR19973073.181140/150‑1 (MQ=60)
                                                                                                                                  CGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCG                     <  SRR19973073.4257894/150‑1 (MQ=60)
                                                                                                                                  CGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCG                     >  SRR19973073.2680718/1‑150 (MQ=60)
                                                                                                                                     ACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTA                  <  SRR19973073.835105/150‑1 (MQ=60)
                                                                                                                                      CCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTAC                 >  SRR19973073.1683643/1‑150 (MQ=60)
                                                                                                                                           GTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTA            <  SRR19973073.824367/150‑1 (MQ=60)
                                                                                                                                            TACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTAC           >  SRR19973073.1602607/1‑150 (MQ=60)
                                                                                                                                            TACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTAC           <  SRR19973073.2157844/150‑1 (MQ=60)
                                                                                                                                              CCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCG         <  SRR19973073.3687671/150‑1 (MQ=60)
                                                                                                                                              CCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCG         <  SRR19973073.3687340/150‑1 (MQ=60)
                                                                                                                                              CCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCG         <  SRR19973073.3687338/150‑1 (MQ=60)
                                                                                                                                                   ATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACC    <  SRR19973073.388744/150‑1 (MQ=60)
                                                                                                                                                   ATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACC    >  SRR19973073.2269552/1‑150 (MQ=60)
                                                                                                                                                     AGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGT  >  SRR19973073.284199/1‑150 (MQ=60)
                                                                                                                                                     AGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGT  >  SRR19973073.2166594/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
ATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGT  >  CP009273/2330353‑2330651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑