Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,846,307 C→A 20.8% A14A (GCG→GCT)  ivbL ← ilvB operon leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,846,3070CA20.8% 749.6 / 139.1 275A14A (GCG→GCT) ivbLilvB operon leader peptide
Reads supporting (aligned to +/- strand):  ref base C (90/127);  new base A (34/23);  total (124/151)
Fisher's exact test for biased strand distribution p-value = 1.68e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

Only 100 of 279 total aligned reads displayed.

CTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTT  >  CP009273/3846159‑3846456
                                                                                                                                                    |                                                                                                                                                     
cttctGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGgcg                                                                                                                                                      <  2:1807837/150‑1 (MQ=255)
          gACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGgtag                                                                                                                                            >  1:1697063/1‑150 (MQ=255)
          gACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGgtag                                                                                                                                            <  2:582501/150‑1 (MQ=255)
           aCTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGTGCAGTTGgtagt                                                                                                                                           <  2:687056/150‑1 (MQ=255)
               tCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTGTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGtt                                                                                                                                       >  2:4068453/1‑150 (MQ=255)
                 cGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGtttt                                                                                                                                     <  2:1719319/150‑1 (MQ=255)
                 cGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGAGCAGTTGGTAGTAGtttt                                                                                                                                     >  2:3488883/1‑150 (MQ=255)
                   ggAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGc                                                                                                                                   >  2:3640443/1‑150 (MQ=255)
                     aGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGt                                                                                                                                 >  2:4127386/1‑150 (MQ=255)
                       gTGCTTAAACGAAAAACCCCGCCCGGTGTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTg                                                                                                                               <  2:1502783/150‑1 (MQ=255)
                            tAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCAt                                                                                                                          >  2:558417/1‑150 (MQ=255)
                            tAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCAt                                                                                                                          <  1:2124447/150‑1 (MQ=255)
                             aaaCGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGAGCAGTTGGTAGTAGTTTTGCGTTGAGCATg                                                                                                                         <  1:2521301/150‑1 (MQ=255)
                               aCGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGa                                                                                                                       <  2:2752550/150‑1 (MQ=255)
                                cGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGaa                                                                                                                      <  1:1057513/150‑1 (MQ=255)
                                  aaaaaCCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGGTGGTAGTAGTTTTGCGTTGAGCATGGAcgt                                                                                                                    >  2:3665540/1‑150 (MQ=255)
                                   aaaaCCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGAGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAagta                                                                                                                   <  2:414157/150‑1 (MQ=255)
                                    aaaCCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAagtag                                                                                                                  <  1:1985855/150‑1 (MQ=255)
                                     aaCCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAagtagt                                                                                                                 <  1:1049346/150‑1 (MQ=255)
                                     aaCCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAagtagt                                                                                                                 <  2:2984139/150‑1 (MQ=255)
                                        cccgcccgGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCAt                                                                                                              <  1:3846670/150‑1 (MQ=255)
                                        cccgcccgGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCAt                                                                                                              <  2:1271285/150‑1 (MQ=255)
                                        cccgcccgGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGGTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCAt                                                                                                              >  1:1319081/1‑150 (MQ=255)
                                         ccgcccgGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGAGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCAtt                                                                                                             >  2:4188737/1‑150 (MQ=255)
                                          ctcccgGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTg                                                                                                            <  2:2232886/148‑1 (MQ=255)
                                          cgcccgGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGAGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTg                                                                                                            >  1:2593423/1‑150 (MQ=255)
                                           gcccgGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGAGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTgg                                                                                                           >  2:731105/1‑150 (MQ=255)
                                           gcccgGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGAGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTgg                                                                                                           >  2:4464654/1‑150 (MQ=255)
                                             ccgGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGAGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTgggg                                                                                                         >  1:325594/1‑150 (MQ=255)
                                             ccgGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGAGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTgggg                                                                                                         >  1:3025509/1‑150 (MQ=255)
                                              cgGTTTGCGCCTGCTGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCTCATTGCCGAAGACCAACACCACACGCACGACGACCACTGCGTCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGATCATGGATGTAGTCATTGGGGa                                                                                                        <  2:1443313/150‑1 (MQ=255)
                                                   tGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCttg                                                                                                   <  1:2689496/150‑1 (MQ=255)
                                                    gcgcCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCttgt                                                                                                  <  1:1400286/150‑1 (MQ=255)
                                                      gcCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCttgttg                                                                                                <  2:2580869/150‑1 (MQ=255)
                                                      gcCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCttgttg                                                                                                >  2:1219292/1‑150 (MQ=255)
                                                       ccGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGAGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTgg                                                                                               >  2:840278/1‑150 (MQ=255)
                                                            ggggTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTtg                                                                                          <  2:3421873/150‑1 (MQ=255)
                                                              ggTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCAGTGGGGACCTTGTTGGTTTtgtg                                                                                        >  2:485291/1‑150 (MQ=255)
                                                              ggTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGAGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTtgtg                                                                                        >  1:307900/1‑150 (MQ=255)
                                                              ggTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGAGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTtgtg                                                                                        >  1:307606/1‑150 (MQ=255)
                                                                 tatgcaaTCGGGTGATGTTCCAGTCCCAACGCCGCCATGCCGACCACCACCACCAAACGCACGACGTCCACTGCGGCGGATGGCGCAGTTGGGAGTAGTTTTGCGTTGAGCATGGAAGTAGTCCTTGGGGACCTTGTTGGTTTTGTGttt                                                                                     >  2:2811448/6‑150 (MQ=255)
                                                                   tGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTaa                                                                                   <  1:1385853/150‑1 (MQ=255)
                                                                    ggAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAAc                                                                                  >  2:4372441/1‑150 (MQ=255)
                                                                       aTCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAat                                                                               <  2:186711/150‑1 (MQ=255)
                                                                           tgtgtTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATAttt                                                                           <  2:379524/150‑1 (MQ=255)
                                                                           tgtgtTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATAttt                                                                           >  2:4534310/1‑150 (MQ=255)
                                                                             tgttgtTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTtat                                                                         >  2:1267106/1‑150 (MQ=255)
                                                                             tgttgtTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTtat                                                                         <  2:771764/150‑1 (MQ=255)
                                                                             tgttgtTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTtat                                                                         <  1:2407288/150‑1 (MQ=255)
                                                                              gttgttCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTtata                                                                        <  2:10958/150‑1 (MQ=255)
                                                                              gttgttCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTtata                                                                        >  1:3480163/1‑150 (MQ=255)
                                                                               ttgttCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGAGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATAc                                                                       >  2:4111524/1‑150 (MQ=255)
                                                                                 gttCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACaa                                                                     >  1:943414/1‑150 (MQ=255)
                                                                                    ccAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGca                                                                  <  1:4432924/150‑1 (MQ=255)
                                                                                    ccAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGca                                                                  <  1:4356368/150‑1 (MQ=255)
                                                                                    ccAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGca                                                                  <  1:1845408/150‑1 (MQ=255)
                                                                                    ccAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGAGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGca                                                                  >  1:4427491/1‑150 (MQ=255)
                                                                                    ccAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGAGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGca                                                                  <  1:3360876/150‑1 (MQ=255)
                                                                                      aGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGcaca                                                                >  1:3055331/1‑150 (MQ=255)
                                                                                       gTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAg                                                               <  1:3256622/150‑1 (MQ=255)
                                                                                       gTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAg                                                               <  1:4151970/150‑1 (MQ=255)
                                                                                       gTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGAGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAg                                                               <  1:3699259/150‑1 (MQ=255)
                                                                                         cccTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCt                                                             <  2:1918639/150‑1 (MQ=255)
                                                                                         cccTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCt                                                             >  2:3254951/1‑150 (MQ=255)
                                                                                         cccTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCt                                                             <  1:1771099/150‑1 (MQ=255)
                                                                                           cTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGAGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCttt                                                           >  2:3698388/1‑150 (MQ=255)
                                                                                            tACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTa                                                          <  1:3972475/150‑1 (MQ=255)
                                                                                              cGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACa                                                        <  2:2603472/150‑1 (MQ=255)
                                                                                              cGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGGTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACa                                                        >  1:2996508/1‑150 (MQ=255)
                                                                                               ggCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGAGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACAg                                                       >  2:216549/1‑150 (MQ=255)
                                                                                               ggCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGAGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACAg                                                       <  2:2043000/150‑1 (MQ=255)
                                                                                                 cgcATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACAggg                                                     <  2:3588082/150‑1 (MQ=255)
                                                                                                      tGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGAGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACAGGGGAGAc                                                <  2:2367426/150‑1 (MQ=255)
                                                                                                        ccGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACAGGGGAGACaa                                              >  1:3291745/1‑150 (MQ=255)
                                                                                                         cgacgaCCACCACCACACGCACGACGACCACTGCGGCGGATGGAGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACAGGGGAGACAAt                                             >  1:1513622/1‑150 (MQ=255)
                                                                                                          gacgacCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACAGGGGAGACAATg                                            >  2:1181249/1‑150 (MQ=255)
                                                                                                            cgacCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACAGGGGAGACAATGGa                                          <  1:3215884/150‑1 (MQ=255)
                                                                                                             gacCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACAGGGGAGACAATGGaa                                         <  1:2278915/150‑1 (MQ=255)
                                                                                                               ccaccaccaCACGCACGACGACCACTGCGGCGGATGGAGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACAGGGGAGACAATGGaaaa                                       >  1:173657/1‑150 (MQ=255)
                                                                                                                        cacgcacgACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACAGGGGAGACAATGGAAAATTTTTCAGc                              >  1:1989324/1‑150 (MQ=255)
                                                                                                                            cacgACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAgg                          <  2:3182135/150‑1 (MQ=255)
                                                                                                                            cacgACGACCACTGCGGCGGATGGAGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAgg                          >  1:4070307/1‑150 (MQ=255)
                                                                                                                             acgacgACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAggg                         <  2:2701047/150‑1 (MQ=255)
                                                                                                                             acgacgACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAggg                         <  1:2059082/150‑1 (MQ=255)
                                                                                                                              cgacgaCCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGTAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACATGCACAGCTTTACAGGGTAGACAATGGATATTTTTTCATCAAGGGa                        <  1:779802/150‑1 (MQ=255)
                                                                                                                               gacgacCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGaa                       >  2:4282559/1‑150 (MQ=255)
                                                                                                                               gacgacCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGaa                       >  1:932981/1‑150 (MQ=255)
                                                                                                                                  gacCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAAt                    >  1:4202907/1‑150 (MQ=255)
                                                                                                                                  gacCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAAt                    >  1:73134/1‑150 (MQ=255)
                                                                                                                                  gacCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAAt                    >  1:1833829/1‑150 (MQ=255)
                                                                                                                                  gacCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAAt                    <  1:3922408/150‑1 (MQ=255)
                                                                                                                                   acCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACCATATTTATACAAGCACAGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAAtt                   >  2:3985727/1‑150 (MQ=255)
                                                                                                                                   acCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAAtt                   <  1:2855248/150‑1 (MQ=255)
                                                                                                                                    ccACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTg                  <  1:4157765/150‑1 (MQ=255)
                                                                                                                                    ccACTGCGGCGGATGGAGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTg                  >  2:1296029/1‑150 (MQ=255)
                                                                                                                                        tGCGGCGGATGGAGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAggg              <  2:249650/150‑1 (MQ=255)
                                                                                                                                             cggATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGa         <  2:616629/150‑1 (MQ=255)
                                                                                                                                              ggATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATTGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGAt        <  1:558417/150‑1 (MQ=255)
                                                                                                                                                aTGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCa      <  1:1337459/150‑1 (MQ=255)
                                                                                                                                                 tGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCAc     >  2:1710172/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTATACAAGCACAGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTT  >  CP009273/3846159‑3846456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A