Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,186,367 G→T 43.3% Q124K (CAA→AAA)  rsd ← stationary phase protein, binds sigma 70 RNA polymerase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,186,3670GT43.3% 299.9 / inf 306Q124K (CAA→AAA) rsdstationary phase protein, binds sigma 70 RNA polymerase subunit
Reads supporting (aligned to +/- strand):  ref base G (80/93);  new base T (67/65);  total (148/158)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 317 total aligned reads displayed.

AATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATGCGCT  >  CP009273/4186218‑4186516
                                                                                                                                                     |                                                                                                                                                     
  tGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTTaa                                                                                                                                                     >  1:2718945/1‑150 (MQ=255)
  tGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTTaa                                                                                                                                                     >  2:4454939/1‑150 (MQ=255)
  tGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTTaa                                                                                                                                                     >  2:3042135/1‑150 (MQ=255)
     ggCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACt                                                                                                                                                  <  1:1692602/150‑1 (MQ=255)
       cATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTTAAACTCa                                                                                                                                                >  2:4197736/1‑150 (MQ=255)
       cATTGAATGTAAATTACGCGTGAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTTAAACTCa                                                                                                                                                <  2:4223123/150‑1 (MQ=255)
         ttGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTTAAACTCAAg                                                                                                                                              <  2:3774152/150‑1 (MQ=255)
          tGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAgg                                                                                                                                             >  2:129069/1‑150 (MQ=255)
           gAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGt                                                                                                                                            >  1:2011226/1‑150 (MQ=255)
            aaTGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTTAAACTCAAGGTa                                                                                                                                           >  2:2972982/1‑150 (MQ=255)
            aaTGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTa                                                                                                                                           >  2:663641/1‑150 (MQ=255)
               gTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAAtt                                                                                                                                        >  1:3280916/1‑150 (MQ=255)
                tAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTTAAACTCAAGGTAATTa                                                                                                                                       >  2:3654999/1‑150 (MQ=255)
                 aaaTTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTAt                                                                                                                                      <  1:4629665/150‑1 (MQ=255)
                  aaTTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATc                                                                                                                                     >  2:2956824/1‑150 (MQ=255)
                    ttACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTTAAACTCAAGGTAATTATCAt                                                                                                                                   <  1:3508941/150‑1 (MQ=255)
                    ttACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCAt                                                                                                                                   <  2:601070/150‑1 (MQ=255)
                      aCGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTTAAACTCAAGGTAATTATCATGa                                                                                                                                 >  1:4567369/1‑150 (MQ=255)
                      aCGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTTAAACTCAAGGTAATTATCATGa                                                                                                                                 <  1:1376172/150‑1 (MQ=255)
                      aCGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGa                                                                                                                                 <  2:3321556/150‑1 (MQ=255)
                       cgcgTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTTAAACTCAAGGTAATTATCATGAt                                                                                                                                >  2:2916974/1‑150 (MQ=255)
                       cgcgTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGAt                                                                                                                                >  2:3885387/1‑150 (MQ=255)
                       cgcgTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGAt                                                                                                                                >  2:1324412/1‑150 (MQ=255)
                         cgTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCGTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCg                                                                                                                              <  2:2120153/150‑1 (MQ=255)
                            tAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATg                                                                                                                           >  2:2509391/1‑150 (MQ=255)
                               cAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCg                                                                                                                        >  1:860784/1‑150 (MQ=255)
                                aGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTTAAACTCAAGGTAATTATCATGATCGATGGCgg                                                                                                                       >  2:3418997/1‑150 (MQ=255)
                                  cgcAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTTAAACTCAAGGTAATTATCATGATCGATGGCGGtt                                                                                                                     >  2:4333771/1‑150 (MQ=255)
                                  cgcAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTTAAACTCAAGGTAATTATCATGATCGATGGCGGtt                                                                                                                     >  1:1182737/1‑150 (MQ=255)
                                    cAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTTAAACTCACGGTAATTATCATGATCGATGGCGGTTTc                                                                                                                   <  2:2818336/150‑1 (MQ=255)
                                     aGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTcc                                                                                                                  <  1:3539295/150‑1 (MQ=255)
                                      gAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTTAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCa                                                                                                                 >  1:739461/1‑150 (MQ=255)
                                           cAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTTAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTg                                                                                                            >  2:130882/1‑150 (MQ=255)
                                            aaGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTTAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTgg                                                                                                           >  1:454456/1‑150 (MQ=255)
                                             aGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTTAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGa                                                                                                          <  1:749145/150‑1 (MQ=255)
                                             aGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTTAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGa                                                                                                          <  1:2839150/150‑1 (MQ=255)
                                                aGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTTAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATc                                                                                                       <  2:4248852/150‑1 (MQ=255)
                                                  gATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCCGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCgt                                                                                                     <  2:3826009/150‑1 (MQ=255)
                                                  gATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTTAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCgt                                                                                                     <  2:1621580/150‑1 (MQ=255)
                                                  gATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCgt                                                                                                     <  2:1435811/150‑1 (MQ=255)
                                                   aTGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTTAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCgta                                                                                                    >  1:2337174/1‑150 (MQ=255)
                                                       ttGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTaa                                                                                                >  2:4094379/1‑150 (MQ=255)
                                                          aCGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATcc                                                                                             >  2:770168/1‑150 (MQ=255)
                                                            gcgggcggCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCAt                                                                                           <  2:2394526/150‑1 (MQ=255)
                                                             cgggcggCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATa                                                                                          <  1:3338730/150‑1 (MQ=255)
                                                             cgggcggCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCGTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATa                                                                                          <  2:1798074/150‑1 (MQ=255)
                                                                    cgTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGtt                                                                                   <  1:2875355/150‑1 (MQ=255)
                                                                     gTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTg                                                                                  <  2:1352345/150‑1 (MQ=255)
                                                                        aaGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTTAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGt                                                                               >  1:4129393/1‑150 (MQ=255)
                                                                        aaGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGt                                                                               <  1:1756467/150‑1 (MQ=255)
                                                                        aaGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGt                                                                               <  1:4014265/150‑1 (MQ=255)
                                                                        aaGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGt                                                                               <  1:3081882/150‑1 (MQ=255)
                                                                         aGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTa                                                                              >  1:3419618/1‑150 (MQ=255)
                                                                             ccAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTTAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTgg                                                                          <  1:418191/150‑1 (MQ=255)
                                                                              cagcagAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTTAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGc                                                                         >  2:1087571/1‑150 (MQ=255)
                                                                                gcagAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCtt                                                                       <  1:3976548/150‑1 (MQ=255)
                                                                                 cagAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCCGCGCCTCGCCGATGTCAGATAAAACCTGTTTAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTc                                                                      <  1:3797437/150‑1 (MQ=255)
                                                                                 cagAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTc                                                                      <  2:3010315/150‑1 (MQ=255)
                                                                                  agAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTTAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCg                                                                     >  2:2080337/1‑150 (MQ=255)
                                                                                  agAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCg                                                                     <  2:2562802/150‑1 (MQ=255)
                                                                                   gAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTTAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGa                                                                    <  1:1548639/150‑1 (MQ=255)
                                                                                     aTCAGCTTATCTACCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGt                                                                  <  1:223621/150‑1 (MQ=255)
                                                                                        aGCTTATCTTCCAGCACAACGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTTAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGc                                                               <  2:1156514/150‑1 (MQ=255)
                                                                                          cTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCgg                                                             >  1:1066641/1‑150 (MQ=255)
                                                                                            tATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTTAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGcc                                                           >  1:4434910/1‑150 (MQ=255)
                                                                                            tATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGcc                                                           >  1:407141/1‑150 (MQ=255)
                                                                                                ttCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAAt                                                       >  1:1289710/1‑150 (MQ=255)
                                                                                                  ccAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCt                                                     <  2:3254205/150‑1 (MQ=255)
                                                                                                    aGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTc                                                   <  2:2650063/150‑1 (MQ=255)
                                                                                                      cacaAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTcgc                                                 >  2:2176655/1‑150 (MQ=255)
                                                                                                          aaGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTTAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCgcggcg                                             <  1:1013739/150‑1 (MQ=255)
                                                                                                          aaGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCgcggcg                                             >  1:374824/1‑150 (MQ=255)
                                                                                                           aGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGgcgc                                            >  2:1885902/1‑150 (MQ=255)
                                                                                                            gcgcgcTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCACGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGgcgcg                                           <  1:265681/150‑1 (MQ=255)
                                                                                                             cgcgcgTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGgcgcgc                                          <  1:545743/150‑1 (MQ=255)
                                                                                                                gcTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGcgcgcgcg                                       <  2:3619326/150‑1 (MQ=255)
                                                                                                                 cTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGa                                      >  2:849998/1‑150 (MQ=255)
                                                                                                                  ttCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAg                                     <  1:3900533/150‑1 (MQ=255)
                                                                                                                       gcgcCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGcc                                <  1:4295563/150‑1 (MQ=255)
                                                                                                                        cgcCTCGCCGATGTCAGATAAAACCTGTTTAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGccc                               <  2:3996124/150‑1 (MQ=255)
                                                                                                                           cTCGCCGATGTCAGATAAAACCTGTTGAAACTCCAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGtt                            <  1:802888/150‑1 (MQ=255)
                                                                                                                             cGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGc                          >  2:2466000/1‑150 (MQ=255)
                                                                                                                                cGATGTCAGATAAAACCTGTTTAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCtt                       >  1:2429900/1‑150 (MQ=255)
                                                                                                                                cGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCtt                       <  2:2323366/150‑1 (MQ=255)
                                                                                                                                    gTCAGATAAAACCTGTTTAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAg                   <  2:2740159/150‑1 (MQ=255)
                                                                                                                                     tCAGATAAAACCTGTTTAAACTCAAGGTAATTATCATGATCGATGGCAGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGc                  <  1:523475/150‑1 (MQ=255)
                                                                                                                                      cAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCt                 >  2:1766938/1‑150 (MQ=255)
                                                                                                                                       aGATAAAACCTGTTTAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCtt                >  2:1457322/1‑150 (MQ=255)
                                                                                                                                       aGATAAAACCTGTTTAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCtt                >  1:2549891/1‑150 (MQ=255)
                                                                                                                                         aTAAAACCTGTTTAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTAt              <  1:2008430/150‑1 (MQ=255)
                                                                                                                                         aTAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATTATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTAt              <  2:2139827/150‑1 (MQ=255)
                                                                                                                                           aaaaCCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATga            <  2:1945086/150‑1 (MQ=255)
                                                                                                                                            aaaCCTGTTTAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATgaa           >  2:4377761/1‑150 (MQ=255)
                                                                                                                                             aaCCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATgaag          <  2:3088409/150‑1 (MQ=255)
                                                                                                                                               ccTGTTTAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATgaagaa        >  2:3141518/1‑150 (MQ=255)
                                                                                                                                                cTGTTTAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAAt       >  2:3845282/1‑150 (MQ=255)
                                                                                                                                                cTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAAt       <  2:460720/150‑1 (MQ=255)
                                                                                                                                                   ttGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATgcg    <  1:3700331/150‑1 (MQ=255)
                                                                                                                                                     gAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATGCGCt  <  1:3927784/150‑1 (MQ=255)
                                                                                                                                                     gAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATGCGCt  <  1:648839/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATGCGCT  >  CP009273/4186218‑4186516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A