Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F218 I0 R1	1506	278.7	9275112	94.5%	8764980	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,236,666	C→A	5.5%	intergenic (‑319/‑46)	malE ← / → malK	maltose transporter subunit/fused maltose transport subunit, ATP‑binding component of ABC superfamily/regulatory protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,236,666	0	C	A	5.5%	902.1 / 17.6	234	intergenic (‑319/‑46)	malE/malK	maltose transporter subunit/fused maltose transport subunit, ATP‑binding component of ABC superfamily/regulatory protein
Reads supporting (aligned to +/- strand): ref base C (110/110); new base A (5/9); total (115/119)
Fisher's exact test for biased strand distribution p-value = 4.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 248 total aligned reads displayed.

CGATTTTTGCAAGCAACATCACGAAATTCCTTACATGACCTCGGTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGAAAGATATCAATCTCGATATCCATGAAGGTGAATTCGTGGTGTTTG  >  CP009273/4236517‑4236814

cGATTTTTGCAAGCAACATCACGAAATTCCTTACATGACCTCGGTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCcatc                                                                                                                                                      >  1:1128573/1‑150 (MQ=255)

cGATTTTTGCAAGCAACATCACGAAATTCCTTACATGACCTCGGTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCcatc                                                                                                                                                      >  2:3946670/1‑150 (MQ=255)

cGATTTTTGCAAGCAACATCACGAAATTCCTTACATGACCTCGGTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCcatc                                                                                                                                                      >  2:3798507/1‑150 (MQ=255)

 gATTTTTGCAAGCAACATCACGAAATTCCTTACATGACCTCGGTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCcatca                                                                                                                                                     >  1:2494445/1‑150 (MQ=255)

   tttttGCAAGCAACATCACGAAATTCCTTACATGACCTCGGTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATg                                                                                                                                                   <  1:304858/150‑1 (MQ=255)

     tttGCAAGCAACATCACGAAATTCCTTTCATGAGCTCGGTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCATCCTCCGCCATAAAAAATCAAGTGGGTTGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGaa                                                                                                                                                 <  2:1026747/150‑1 (MQ=255)

      ttGCAAGCAACATCACGAAATTCCTTACATGACCTCGGTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAAt                                                                                                                                                <  1:2802947/150‑1 (MQ=255)

         caagcaaCATCACGAAATTCCTTACATGACCTCGGTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATAATGAATGtt                                                                                                                                             <  2:4625356/150‑1 (MQ=255)

         caagcaaCATCACGAAATTCCTTACATGACCTCGGTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATAATGAATGtt                                                                                                                                             <  2:3877514/150‑1 (MQ=255)

          aagcaaCATCACGAAATTCCTTACATGACCTCGGTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTg                                                                                                                                            >  2:4542691/1‑150 (MQ=255)

           agcaaCATCACGAAATTCCTTACATGACCTCGGTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGc                                                                                                                                           >  2:1604428/1‑150 (MQ=255)

            gcaaCATCACGAAATTCCTTACATGACCTCGGTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCt                                                                                                                                          >  2:2647052/1‑150 (MQ=255)

              aaCATCACGAAATTCCTTACATGACCTCGGTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGt                                                                                                                                        <  1:1854279/150‑1 (MQ=255)

                     cGAAATTCCTTACATGACCTCGGTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGAc                                                                                                                                 <  1:874392/150‑1 (MQ=255)

                      gAAATTCCTTACATGACCTCGGTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACa                                                                                                                                >  1:2362013/1‑150 (MQ=255)

                       aaaTTCCTTACATGACCTCGGTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAg                                                                                                                               >  1:752927/1‑150 (MQ=255)

                              ttACATGACCTCGGTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTa                                                                                                                        >  2:1917768/1‑150 (MQ=255)

                              ttACATGACCTCGGTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTa                                                                                                                        >  1:4204776/1‑150 (MQ=255)

                               tACATGACCTCGGTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTAc                                                                                                                       <  1:126122/150‑1 (MQ=255)

                                aCATGACCTCGGTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACa                                                                                                                      <  2:1681351/150‑1 (MQ=255)

                                aCATGACCTCGGTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACa                                                                                                                      <  1:365475/150‑1 (MQ=255)

                                aCATGACCTCGGTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACa                                                                                                                      >  1:1049288/1‑150 (MQ=255)

                                aCATGACCTCGGTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACa                                                                                                                      >  1:1049192/1‑150 (MQ=255)

                                  aTGACCTCGGTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACaaa                                                                                                                    >  1:289257/1‑150 (MQ=255)

                                   tGACCTCGGTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAg                                                                                                                   <  1:4038519/150‑1 (MQ=255)

                                       cTCGGTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGgag                                                                                                               <  1:3493199/150‑1 (MQ=255)

                                        tCGGTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGgaga                                                                                                              <  1:3542382/150‑1 (MQ=255)

                                        tCGGTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGgaga                                                                                                              <  1:3542191/150‑1 (MQ=255)

                                          ggTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAg                                                                                                            <  2:874480/150‑1 (MQ=255)

                                          ggTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAg                                                                                                            <  2:322842/150‑1 (MQ=255)

                                          ggTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAg                                                                                                            <  1:2403635/150‑1 (MQ=255)

                                               aGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCAt                                                                                                       <  1:1375128/150‑1 (MQ=255)

                                                  tCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGc                                                                                                    <  2:2221934/150‑1 (MQ=255)

                                                  tCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGc                                                                                                    <  1:4216019/150‑1 (MQ=255)

                                                      aGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGc                                                                                                >  1:837531/1‑150 (MQ=255)

                                                      aGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGc                                                                                                >  2:371438/1‑150 (MQ=255)

                                                        aaGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGt                                                                                              >  1:1254460/1‑150 (MQ=255)

                                                         aGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTa                                                                                             <  1:1282809/150‑1 (MQ=255)

                                                          gCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCCTCTCCTGATGACGCATAGTCAGCCCATAATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTAc                                                                                            <  2:2997747/150‑1 (MQ=255)

                                                            cGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAg                                                                                          <  1:2809949/150‑1 (MQ=255)

                                                               gtTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTg                                                                                       <  1:3854168/150‑1 (MQ=255)

                                                                 tctcATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCa                                                                                     >  2:1458855/1‑150 (MQ=255)

                                                                  ctcATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCaa                                                                                    <  2:3513152/150‑1 (MQ=255)

                                                                  ctcATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCaa                                                                                    >  1:1550908/1‑150 (MQ=255)

                                                                     aTCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAAt                                                                                 >  1:2005229/1‑150 (MQ=255)

                                                                       cctccCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGt                                                                               >  2:2579202/1‑150 (MQ=255)

                                                                        ctccCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTa                                                                              <  2:3518298/150‑1 (MQ=255)

                                                                          cccGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTCCAGCTGCAAAATGTAAc                                                                            >  1:3609818/1‑150 (MQ=255)

                                                                            cGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGa                                                                          >  2:2715042/1‑150 (MQ=255)

                                                                            cGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGa                                                                          <  2:1333952/150‑1 (MQ=255)

                                                                              cctcctCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGaaa                                                                        <  2:19402/150‑1 (MQ=255)

                                                                              cctcctCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGaaa                                                                        >  1:4037119/1‑150 (MQ=255)

                                                                                tcctccCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGc                                                                      >  2:2909015/1‑150 (MQ=255)

                                                                                  ctccCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCt                                                                    <  2:525838/150‑1 (MQ=255)

                                                                                   tccCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTg                                                                   <  1:3823939/150‑1 (MQ=255)

                                                                                   tccCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTg                                                                   <  2:3478297/150‑1 (MQ=255)

                                                                                       ccATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGc                                                               >  2:3278841/1‑150 (MQ=255)

                                                                                          tAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAg                                                            >  1:3380329/1‑150 (MQ=255)

                                                                                             aaaaGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTc                                                         <  1:2992351/150‑1 (MQ=255)

                                                                                             aaaaGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTc                                                         <  1:4221290/150‑1 (MQ=255)

                                                                                              aaaGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCg                                                        <  1:3162765/150‑1 (MQ=255)

                                                                                                  ccAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGt                                                    <  2:3211899/150‑1 (MQ=255)

                                                                                                  ccAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGt                                                    >  2:1179386/1‑150 (MQ=255)

                                                                                                    aGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTAt                                                  >  1:635724/1‑150 (MQ=255)

                                                                                                    aGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTAt                                                  >  2:2169071/1‑150 (MQ=255)

                                                                                                     gggggTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATc                                                 <  1:2746339/150‑1 (MQ=255)

                                                                                                      ggggTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCg                                                <  1:678925/150‑1 (MQ=255)

                                                                                                        ggTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGaa                                              <  2:4122790/150‑1 (MQ=255)

                                                                                                        ggTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGaa                                              >  2:680292/1‑150 (MQ=255)

                                                                                                         gTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGaaa                                             >  1:478014/1‑150 (MQ=255)

                                                                                                           ggaggaTTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGAAAGa                                           >  1:4521518/1‑150 (MQ=255)

                                                                                                             aggaTTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGAAAGata                                         >  2:4198089/1‑150 (MQ=255)

                                                                                                              ggaTTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGAAAGatat                                        >  1:3970018/1‑150 (MQ=255)

                                                                                                               gaTTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCTAAAGATATc                                       <  1:2137291/150‑1 (MQ=255)

                                                                                                                    aaGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGAAAGATATCAAtct                                  >  1:1730854/1‑150 (MQ=255)

                                                                                                                     aGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGCTGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACCGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGAAAGATATCAAtctc                                 >  2:2779660/1‑150 (MQ=255)

                                                                                                                     aGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGAAAGATATCAAtctc                                 >  1:3469275/1‑150 (MQ=255)

                                                                                                                      gCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGAGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGAAAGATATCAATCTCg                                >  2:1306823/1‑150 (MQ=255)

                                                                                                                      gCCATCTCCTGATGACGCATAGTCAGCCCATAATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGAAAGATATCAATCTCg                                <  2:4493008/150‑1 (MQ=255)

                                                                                                                       ccATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGAAAGATATCAATCTCGa                               >  2:2738645/1‑150 (MQ=255)

                                                                                                                         aTCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGAAAGATATCAATCTCGata                             <  2:1992198/150‑1 (MQ=255)

                                                                                                                           ctcCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGAAAGATATCAATCTCGATATc                           >  1:2045591/1‑150 (MQ=255)

                                                                                                                              cTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGAAAGATATCAATCTCGATATCCAt                        >  1:1466400/1‑150 (MQ=255)

                                                                                                                                gatgaCGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGAAAGATATCAATCTCGATATCCATGa                      >  2:1592154/1‑150 (MQ=255)

                                                                                                                                 atgaCGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGAAAGATATCAATCTCGATATCCATGaa                     <  1:2918398/150‑1 (MQ=255)

                                                                                                                                  tgaCGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGAAAGATATCAATCTCGATATCCATGacg                    <  2:2868224/150‑3 (MQ=255)

                                                                                                                                   gaCGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGAAAGATATCAATCTCGATATCCATGAAgg                   <  2:3671168/150‑1 (MQ=255)

                                                                                                                                    aCGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGAAAGATATCAATCTCGATATCCATGAAGGt                  >  1:4156065/1‑150 (MQ=255)

                                                                                                                                    aCGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGAAAGATATCAATCTCGATATCCATGAAGGt                  <  2:3055046/150‑1 (MQ=255)

                                                                                                                                        aTAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGAAAGATATCAATCTCGATATCCATGAAGGTGAAt              <  1:3274066/150‑1 (MQ=255)

                                                                                                                                        aTAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGAAAGATATCAATCTCGATATCCATGAAGGTGAAt              <  2:2526688/150‑1 (MQ=255)

                                                                                                                                          aGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGAAAGATATCAATCTCGATATCCATGAAGGTGAATTc            >  1:3886474/1‑150 (MQ=255)

                                                                                                                                             cAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGAAAGATATCAATCTCGATATCCATGAAGGTGAATTCgtg         <  2:1920784/150‑1 (MQ=255)

                                                                                                                                             cAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGAAAGATATCAATCTCGATATCCATGAAGGTGAAGTCgtg         >  2:825994/1‑150 (MQ=255)

                                                                                                                                              aGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGAAAGATATCAATCTCGATATCCATGAAGGTGAATTCgtgg        <  1:1449212/150‑1 (MQ=255)

                                                                                                                                              aGCCCATAATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGAAAGATATCAATCTCGATATCCATGAAGGTGAATTCgtgg        >  2:2258498/1‑150 (MQ=255)

                                                                                                                                               gCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGAAAGATATCAATCTCGATATCCATGAAGGTGAATTCgtggt       <  1:1599176/150‑1 (MQ=255)

                                                                                                                                               gCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGAAAGATATCAATCTCGATATCCATGAAGGTGAATTCgtggt       <  2:855910/150‑1 (MQ=255)

                                                                                                                                                  catcatGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGAAAGATATCAATCTCGATATCCATGAAGGTGAATTCGTGGTGtt    >  1:2663683/1‑150 (MQ=255)

                                                                                                                                                    tcatGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGAAAGATATCAATCTCGATATCCATGAAGGTGAATTCGTGGTGTTTg  <  2:4034609/150‑1 (MQ=255)

CGATTTTTGCAAGCAACATCACGAAATTCCTTACATGACCTCGGTTTAGTTCACAGAAGCCGTGTTCTCATCCTCCCGCCTCCTCCCCCATAAAAAAGCCAGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATCATGAATGTTGCTGTCGATGACAGGTTGTTACAAAGGGAGAAGGGCATGGCGAGCGTACAGCTGCAAAATGTAACGAAAGCCTGGGGCGAGGTCGTGGTATCGAAAGATATCAATCTCGATATCCATGAAGGTGAATTCGTGGTGTTTG  >  CP009273/4236517‑4236814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A