Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 279,800 G→T 100% E297* (GAA→TAA)  yagF → CP4‑6 prophage; dehydratase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273279,8000GT98.1% 396.1 / ‑1.0 125E297* (GAA→TAA) yagFCP4‑6 prophage; dehydratase family protein
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (61/61);  total (62/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 125 total aligned reads displayed.

CTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGA  >  CP009273/279656‑279944
                                                                                                                                                |                                                                                                                                                
cTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTaaa                                                                                                                                               <  1:3249849/148‑1 (MQ=255)
cTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTaaa                                                                                                                                               <  1:2837064/148‑1 (MQ=255)
cTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTaaa                                                                                                                                               <  2:2664972/148‑1 (MQ=255)
     tCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAAcgcg                                                                                                                                          <  1:2425211/148‑1 (MQ=255)
     tCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAAcgcg                                                                                                                                          <  1:2424746/148‑1 (MQ=255)
          cgcTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGt                                                                                                                                     <  1:1015240/148‑1 (MQ=255)
           gcTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTg                                                                                                                                    <  2:1095006/148‑1 (MQ=255)
            cTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGa                                                                                                                                   <  1:927792/148‑1 (MQ=255)
            cTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGa                                                                                                                                   <  2:300228/148‑1 (MQ=255)
             tGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGAt                                                                                                                                  <  2:297905/148‑1 (MQ=255)
              gCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATc                                                                                                                                 <  1:3617131/148‑1 (MQ=255)
              gCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATc                                                                                                                                 <  1:3617125/148‑1 (MQ=255)
                      ccGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACgcggc                                                                                                                         >  2:1351370/1‑148 (MQ=255)
                      ccGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACgcggc                                                                                                                         >  2:1351418/1‑148 (MQ=255)
                      ccGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACgcggc                                                                                                                         >  2:2936370/1‑148 (MQ=255)
                      ccGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACgcggc                                                                                                                         >  2:1351424/1‑148 (MQ=255)
                       cgcgCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACgcggcg                                                                                                                        <  2:3712867/148‑1 (MQ=255)
                       cgcgCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACgcggcg                                                                                                                        <  2:3712816/148‑1 (MQ=255)
                       cgcgCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACgcggcg                                                                                                                        <  1:1220409/148‑1 (MQ=255)
                        gcgcTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGt                                                                                                                       <  1:3314225/148‑1 (MQ=255)
                        gcgcTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGt                                                                                                                       <  1:293605/148‑1 (MQ=255)
                        gcgcTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGt                                                                                                                       <  1:295004/148‑1 (MQ=255)
                         cgcTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGtt                                                                                                                      <  1:325574/148‑1 (MQ=255)
                         cgcTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGtt                                                                                                                      <  1:325513/148‑1 (MQ=255)
                         cgcTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGtt                                                                                                                      <  1:325509/148‑1 (MQ=255)
                         cgcTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGtt                                                                                                                      <  1:3543988/148‑1 (MQ=255)
                          gcTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTc                                                                                                                     <  2:2838582/148‑1 (MQ=255)
                               cgcCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTcggcgg                                                                                                                <  1:1987491/148‑1 (MQ=255)
                                 ccGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCt                                                                                                              <  2:134442/148‑1 (MQ=255)
                                 ccGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCt                                                                                                              <  1:769700/148‑1 (MQ=255)
                                  cGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGAGAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTc                                                                                                             <  1:1303639/148‑1 (MQ=255)
                                   gTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTcc                                                                                                            <  2:2090725/148‑1 (MQ=255)
                                        ggCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCTCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCaa                                                                                                       <  2:2572473/148‑1 (MQ=255)
                                        ggCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAACCGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCaa                                                                                                       <  1:2660871/148‑1 (MQ=255)
                                        ggCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCaa                                                                                                       >  1:3184798/1‑148 (MQ=255)
                                        ggCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCaa                                                                                                       <  2:2572206/148‑1 (MQ=255)
                                         gCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAAt                                                                                                      <  1:121190/148‑1 (MQ=255)
                                            ggCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTa                                                                                                   <  1:1371003/148‑1 (MQ=255)
                                              cGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTAct                                                                                                 >  2:2176417/1‑148 (MQ=255)
                                                gtgtGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTActgc                                                                                               >  2:2703688/1‑148 (MQ=255)
                                                 tgtgGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTActgct                                                                                              >  1:767165/1‑148 (MQ=255)
                                                      cTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGcaca                                                                                         <  2:412399/148‑1 (MQ=255)
                                                        ggAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACAtt                                                                                       <  1:938328/148‑1 (MQ=255)
                                                          agaTCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTcc                                                                                     >  1:696270/1‑148 (MQ=255)
                                                            aTCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCgg                                                                                   >  1:140952/1‑148 (MQ=255)
                                                            aTCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAAAGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCgg                                                                                   >  1:141061/1‑148 (MQ=255)
                                                             tCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGc                                                                                  <  2:730685/148‑1 (MQ=255)
                                                             tCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGc                                                                                  <  2:904912/148‑1 (MQ=255)
                                                                cccgccAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCAt                                                                               >  1:1004813/1‑148 (MQ=255)
                                                                  cgccAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCg                                                                             >  2:3187445/1‑148 (MQ=255)
                                                                      aGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCa                                                                         >  1:1764988/1‑148 (MQ=255)
                                                                       gTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCAc                                                                        >  2:2686411/1‑148 (MQ=255)
                                                                        tCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCAcg                                                                       >  1:1015139/1‑148 (MQ=255)
                                                                           gcgcgcgcGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACgcgg                                                                    <  2:293445/148‑1 (MQ=255)
                                                                                 gcgGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCt                                                              <  2:1935554/148‑1 (MQ=255)
                                                                                      cAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACg                                                         >  2:3017366/1‑148 (MQ=255)
                                                                                        gCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGAt                                                       >  2:859596/1‑148 (MQ=255)
                                                                                        gCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGAt                                                       >  2:859120/1‑148 (MQ=255)
                                                                                            gCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCg                                                   >  1:1317208/1‑148 (MQ=255)
                                                                                             cTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCgg                                                  <  1:393276/148‑1 (MQ=255)
                                                                                             cTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCgg                                                  <  2:1200115/148‑1 (MQ=255)
                                                                                                gATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACg                                               >  1:354936/1‑148 (MQ=255)
                                                                                                 aTAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGt                                              >  2:3796523/1‑148 (MQ=255)
                                                                                                     ccGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAg                                          >  2:438341/1‑148 (MQ=255)
                                                                                                     ccGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAg                                          >  2:508332/1‑148 (MQ=255)
                                                                                                     ccGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAg                                          >  1:2359208/1‑148 (MQ=255)
                                                                                                       gcgGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCa                                        <  1:2951997/148‑1 (MQ=255)
                                                                                                       gcgGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCa                                        <  1:2951645/148‑1 (MQ=255)
                                                                                                          gCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTg                                     <  1:3580226/148‑1 (MQ=255)
                                                                                                                ccacGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGAcgcg                               >  1:982931/1‑148 (MQ=255)
                                                                                                                  acGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCa                             >  1:510928/1‑148 (MQ=255)
                                                                                                                   cgcgGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCAt                            >  2:1519898/1‑148 (MQ=255)
                                                                                                                   cgcgGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCAt                            >  2:1519835/1‑148 (MQ=255)
                                                                                                                        gATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAAcc                       <  1:2603802/148‑1 (MQ=255)
                                                                                                                        gATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAAcc                       >  1:3783619/1‑148 (MQ=255)
                                                                                                                        gATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAAcc                       >  1:3782955/1‑148 (MQ=255)
                                                                                                                         atatCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCg                      >  2:1268834/1‑148 (MQ=255)
                                                                                                                           atCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTa                    >  1:445998/1‑148 (MQ=255)
                                                                                                                               tctcCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGt                >  2:3446961/1‑148 (MQ=255)
                                                                                                                               tctcCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGt                >  2:1418907/1‑148 (MQ=255)
                                                                                                                                ctcCGATAAAGCCATCTAAAACGCGGTGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTg               >  2:1123319/1‑148 (MQ=255)
                                                                                                                                ctcCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTg               >  1:3424666/1‑148 (MQ=255)
                                                                                                                                  ccGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGcc             >  2:1470045/1‑148 (MQ=255)
                                                                                                                                      tAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGt         <  1:3149687/148‑1 (MQ=255)
                                                                                                                                      tAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGt         <  1:137126/148‑1 (MQ=255)
                                                                                                                                      tAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGt         <  2:3394524/148‑1 (MQ=255)
                                                                                                                                       aaaGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTc        >  1:2148266/1‑148 (MQ=255)
                                                                                                                                         aGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTg      >  2:583488/1‑148 (MQ=255)
                                                                                                                                         aGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTg      >  2:1810460/1‑148 (MQ=255)
                                                                                                                                          gCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTgg     >  1:2920757/1‑148 (MQ=255)
                                                                                                                                          gCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTgg     <  1:1736202/148‑1 (MQ=255)
                                                                                                                                          gCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTgg     <  1:1736229/148‑1 (MQ=255)
                                                                                                                                          gCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTgg     >  1:1043596/1‑148 (MQ=255)
                                                                                                                                          gCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTgg     >  1:224586/1‑148 (MQ=255)
                                                                                                                                          gCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTgg     >  1:224597/1‑148 (MQ=255)
                                                                                                                                          gCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTgg     >  1:645705/1‑148 (MQ=255)
                                                                                                                                          gCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAACTGCCGCGTCTgg     >  1:968427/1‑148 (MQ=255)
                                                                                                                                           ccATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGt    >  2:3695317/1‑148 (MQ=255)
                                                                                                                                             aTCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGa  >  2:285870/1‑148 (MQ=255)
                                                                                                                                             aTCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGa  >  2:285170/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGA  >  CP009273/279656‑279944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCGCTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGC  >  CP009273/279652‑279946
                                                                                                                                                    |                                                                                                                                                  
GGCGCTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTA                                                                                                                                                   <  SRR19973069.3400545/150‑1 (MQ=60)
GGCGCTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTA                                                                                                                                                   <  SRR19973069.3400548/150‑1 (MQ=60)
    CTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAAC                                                                                                                                               <  SRR19973069.2855793/150‑1 (MQ=60)
    CTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAAC                                                                                                                                               <  SRR19973069.3271124/150‑1 (MQ=60)
    CTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAAC                                                                                                                                               <  SRR19973069.2380533/150‑1 (MQ=60)
         TCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGAT                                                                                                                                          <  SRR19973069.2441579/150‑1 (MQ=60)
         TCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGAT                                                                                                                                          <  SRR19973069.2441112/150‑1 (MQ=60)
              CGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGA                                                                                                                                     <  SRR19973069.1022242/150‑1 (MQ=60)
                CTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATC                                                                                                                                   <  SRR19973069.934095/150‑1 (MQ=60)
                  GCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCA                                                                                                                                 <  SRR19973069.3640750/150‑1 (MQ=60)
                  GCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCA                                                                                                                                 <  SRR19973069.3640756/150‑1 (MQ=60)
                           CGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTT                                                                                                                        <  SRR19973069.1228755/150‑1 (MQ=60)
                            GCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTC                                                                                                                       <  SRR19973069.297077/150‑1 (MQ=60)
                            GCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTC                                                                                                                       <  SRR19973069.3335952/150‑1 (MQ=60)
                            GCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTC                                                                                                                       <  SRR19973069.295619/150‑1 (MQ=60)
                            GCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTC                                                                                                                       <  SRR19973069.297023/150‑1 (MQ=60)
                             CGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCG                                                                                                                      <  SRR19973069.3567001/150‑1 (MQ=60)
                             CGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCG                                                                                                                      <  SRR19973069.327696/150‑1 (MQ=60)
                             CGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCG                                                                                                                      <  SRR19973069.327700/150‑1 (MQ=60)
                             CGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCG                                                                                                                      <  SRR19973069.327761/150‑1 (MQ=60)
                                   CGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCT                                                                                                                <  SRR19973069.2001338/150‑1 (MQ=60)
                                     CCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCC                                                                                                              <  SRR19973069.775138/150‑1 (MQ=60)
                                      CGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCA                                                                                                             <  SRR19973069.3228347/150‑1 (MQ=60)
                                      CGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGAGAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCA                                                                                                             <  SRR19973069.1312483/150‑1 (MQ=60)
                                          CGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAA                                                                                                         >  SRR19973069.3205495/1‑150 (MQ=60)
                                            GGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAACCGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATT                                                                                                       <  SRR19973069.2678710/150‑1 (MQ=60)
                                             GCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTT                                                                                                      <  SRR19973069.122341/150‑1 (MQ=60)
                                                GGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACT                                                                                                   <  SRR19973069.1380542/150‑1 (MQ=60)
                                                   GGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCT                                                                                                >  SRR19973069.772581/1‑150 (MQ=60)
                                                            GGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCC                                                                                       >  SRR19973069.701159/1‑150 (MQ=60)
                                                            GGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCC                                                                                       <  SRR19973069.944674/150‑1 (MQ=60)
                                                              AGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGG                                                                                     >  SRR19973069.142238/1‑150 (MQ=60)
                                                              AGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAAAGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGG                                                                                     >  SRR19973069.142348/1‑150 (MQ=60)
                                                                  CGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCAT                                                                                 >  SRR19973069.1011715/1‑150 (MQ=60)
                                                                        CCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCA                                                                           >  SRR19973069.1777432/1‑150 (MQ=60)
                                                                          AGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACG                                                                         >  SRR19973069.1022140/1‑150 (MQ=60)
                                                                                  CGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGC                                                                 >  SRR19973069.1208366/1‑150 (MQ=60)
                                                                                       GGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCAC                                                            >  SRR19973069.1332825/1‑150 (MQ=60)
                                                                                              GAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCG                                                     >  SRR19973069.1326247/1‑150 (MQ=60)
                                                                                                 CTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGAC                                                  <  SRR19973069.396168/150‑1 (MQ=60)
                                                                                                  TGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACG                                                 >  SRR19973069.357538/1‑150 (MQ=60)
                                                                                                  TGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACG                                                 >  SRR19973069.357474/1‑150 (MQ=60)
                                                                                                       AGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAG                                            >  SRR19973069.2375234/1‑150 (MQ=60)
                                                                                                           GCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACT                                        <  SRR19973069.2971230/150‑1 (MQ=60)
                                                                                                           GCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACT                                        <  SRR19973069.2971584/150‑1 (MQ=60)
                                                                                                              GCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGA                                     <  SRR19973069.3603571/150‑1 (MQ=60)
                                                                                                                  CACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCG                                 >  SRR19973069.989573/1‑150 (MQ=60)
                                                                                                                  CACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCG                                 <  SRR19973069.2768605/150‑1 (MQ=60)
                                                                                                                    CCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCA                               >  SRR19973069.514563/1‑150 (MQ=60)
                                                                                                                         CGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAAC                          <  SRR19973069.3300311/150‑1 (MQ=60)
                                                                                                                          GGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACC                         >  SRR19973069.3808172/1‑150 (MQ=60)
                                                                                                                          GGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACC                         <  SRR19973069.887485/150‑1 (MQ=60)
                                                                                                                          GGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACC                         >  SRR19973069.3807544/1‑150 (MQ=60)
                                                                                                                          GGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACC                         >  SRR19973069.3807508/1‑150 (MQ=60)
                                                                                                                            GATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGT                       <  SRR19973069.2621185/150‑1 (MQ=60)
                                                                                                                             ATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTA                      >  SRR19973069.170788/1‑150 (MQ=60)
                                                                                                                             ATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTA                      >  SRR19973069.449261/1‑150 (MQ=60)
                                                                                                                              TATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAA                     >  SRR19973069.524285/1‑150 (MQ=60)
                                                                                                                                  CTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTG                 >  SRR19973069.3446943/1‑150 (MQ=60)
                                                                                                                                         ATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTC          >  SRR19973069.2161528/1‑150 (MQ=60)
                                                                                                                                         ATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTC          >  SRR19973069.2162991/1‑150 (MQ=60)
                                                                                                                                          TAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCT         <  SRR19973069.138394/150‑1 (MQ=60)
                                                                                                                                          TAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCT         <  SRR19973069.3170273/150‑1 (MQ=60)
                                                                                                                                            AAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGG       >  SRR19973069.226320/1‑150 (MQ=60)
                                                                                                                                            AAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGG       >  SRR19973069.226331/1‑150 (MQ=60)
                                                                                                                                            AAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGG       >  SRR19973069.2940101/1‑150 (MQ=60)
                                                                                                                                            AAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGG       >  SRR19973069.649955/1‑150 (MQ=60)
                                                                                                                                            AAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGG       >  SRR19973069.1050846/1‑150 (MQ=60)
                                                                                                                                            AAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAACTGCCGCGTCTGG       >  SRR19973069.974880/1‑150 (MQ=60)
                                                                                                                                              GCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTG     <  SRR19973069.1748373/150‑1 (MQ=60)
                                                                                                                                              GCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTG     <  SRR19973069.1748402/150‑1 (MQ=60)
                                                                                                                                                 ATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGC  >  SRR19973069.2427463/1‑150 (MQ=60)
                                                                                                                                                 ATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGC  >  SRR19973069.1619039/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                  
GGCGCTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGC  >  CP009273/279652‑279946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑