Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,249,227 G→T 100% R728S (CGT→AGT)  ycgV ← putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,249,2270GT98.8% 580.9 / ‑1.5 182R728S (CGT→AGT) ycgVputative adhesin
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (81/97);  total (83/99)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 184 total aligned reads displayed.

TGTAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCATCCGTT  >  CP009273/1249083‑1249370
                                                                                                                                                |                                                                                                                                               
tGTAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACtttt                                                                                                                                              <  2:2382705/148‑1 (MQ=255)
 gTAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTa                                                                                                                                             <  1:3495746/148‑1 (MQ=255)
  tAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTAt                                                                                                                                            <  1:2044592/148‑1 (MQ=255)
  tAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTAt                                                                                                                                            <  1:894463/148‑1 (MQ=255)
  tAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTAt                                                                                                                                            <  1:1745014/148‑1 (MQ=255)
   aaaaaCCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATc                                                                                                                                           <  1:394788/148‑1 (MQ=255)
    aaaaCCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATcc                                                                                                                                          <  1:138690/148‑1 (MQ=255)
      aaCCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATcccc                                                                                                                                        >  2:2771848/1‑148 (MQ=255)
           ttttGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCaa                                                                                                                                   <  1:1185033/148‑1 (MQ=255)
           ttttGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCaa                                                                                                                                   >  2:1145116/1‑148 (MQ=255)
            tttGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCaaa                                                                                                                                  >  2:2793918/1‑148 (MQ=255)
                tgCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTgg                                                                                                                              <  2:2159259/148‑1 (MQ=255)
                  ccATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGAt                                                                                                                            >  1:2927126/1‑148 (MQ=255)
                  ccATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGAt                                                                                                                            <  1:2109456/148‑1 (MQ=255)
                      gTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCg                                                                                                                        <  1:1884479/148‑1 (MQ=255)
                          cTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGt                                                                                                                    >  1:794292/1‑148 (MQ=255)
                          cTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGt                                                                                                                    >  1:488133/1‑148 (MQ=255)
                            ggCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTaa                                                                                                                  <  2:3128049/148‑1 (MQ=255)
                                tACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCa                                                                                                              <  1:1453257/148‑1 (MQ=255)
                                tACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCa                                                                                                              <  1:495083/148‑1 (MQ=255)
                                    ttCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTc                                                                                                          >  2:147865/1‑148 (MQ=255)
                                    ttCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTc                                                                                                          >  2:2073749/1‑148 (MQ=255)
                                     tCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCa                                                                                                         <  2:967543/148‑1 (MQ=255)
                                     tCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCa                                                                                                         >  1:1330666/1‑148 (MQ=255)
                                     tCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCa                                                                                                         >  1:1591046/1‑148 (MQ=255)
                                      cccATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCaa                                                                                                        <  1:3226751/148‑1 (MQ=255)
                                         aTGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGc                                                                                                     >  2:1818439/1‑148 (MQ=255)
                                         aTGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGc                                                                                                     >  2:3283138/1‑148 (MQ=255)
                                         aTGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGc                                                                                                     >  2:3283125/1‑148 (MQ=255)
                                         aTGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGc                                                                                                     >  2:1818431/1‑148 (MQ=255)
                                             aGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCt                                                                                                 <  2:535369/148‑1 (MQ=255)
                                             aGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCt                                                                                                 <  2:2454248/148‑1 (MQ=255)
                                                cTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAg                                                                                              <  1:951577/148‑1 (MQ=255)
                                                 tGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGt                                                                                             <  1:171714/148‑1 (MQ=255)
                                                 tGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGt                                                                                             <  1:3262806/148‑1 (MQ=255)
                                                 tGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGt                                                                                             <  1:3348282/148‑1 (MQ=255)
                                                  gAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGtt                                                                                            >  1:638094/1‑148 (MQ=255)
                                                    aCGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTg                                                                                          >  2:416089/1‑148 (MQ=255)
                                                       tGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCa                                                                                       <  1:1015841/148‑1 (MQ=255)
                                                        gCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCAc                                                                                      <  2:837468/148‑1 (MQ=255)
                                                            tACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGc                                                                                  >  2:1936775/1‑148 (MQ=255)
                                                            tACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGc                                                                                  >  2:1936779/1‑148 (MQ=255)
                                                              ccGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCCACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCaa                                                                                <  1:1387585/148‑1 (MQ=255)
                                                               cGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCaaa                                                                               <  1:706484/148‑1 (MQ=255)
                                                                 tCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAgg                                                                             <  2:1001510/148‑1 (MQ=255)
                                                                   gCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAg                                                                           <  2:746656/148‑1 (MQ=255)
                                                                     cTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTc                                                                         <  1:2156166/148‑1 (MQ=255)
                                                                     cTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTc                                                                         <  2:3500349/148‑1 (MQ=255)
                                                                     cTCCGCTATAGTCCGGCGATGCATGTGTTGAGCAAATATACAGACCGACATACAACGGAATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTc                                                                         <  1:2156187/148‑1 (MQ=255)
                                                                      tCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTcc                                                                        >  1:2310544/1‑148 (MQ=255)
                                                                      tCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTcc                                                                        >  2:3227551/1‑148 (MQ=255)
                                                                          cTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGc                                                                    <  2:218947/148‑1 (MQ=255)
                                                                          cTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGc                                                                    <  1:2606410/148‑1 (MQ=255)
                                                                           tataGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCt                                                                   >  2:352831/1‑148 (MQ=255)
                                                                            ataGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCtt                                                                  >  2:1781988/1‑148 (MQ=255)
                                                                              aGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACACCGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTcc                                                                <  2:2760460/148‑1 (MQ=255)
                                                                               gTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTccc                                                               >  1:3044149/1‑148 (MQ=255)
                                                                                tCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTcccc                                                              >  2:2612600/1‑148 (MQ=255)
                                                                                  cGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCa                                                            >  2:1451475/1‑148 (MQ=255)
                                                                                    gCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATa                                                          <  2:1682932/148‑1 (MQ=255)
                                                                                    gCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATa                                                          <  2:3577784/148‑1 (MQ=255)
                                                                                    gCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGAATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATa                                                          <  1:2469357/148‑1 (MQ=255)
                                                                                         gCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTgc                                                     >  2:2888635/1‑148 (MQ=255)
                                                                                         gCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTgc                                                     >  2:2625413/1‑148 (MQ=255)
                                                                                          cATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTgcg                                                    >  1:304500/1‑148 (MQ=255)
                                                                                                 tGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAg                                             <  1:1166058/148‑1 (MQ=255)
                                                                                                     ccAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGatat                                         <  1:1062354/148‑1 (MQ=255)
                                                                                                     ccAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGatat                                         <  1:2370466/148‑1 (MQ=255)
                                                                                                      cAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGTCTTCCCCCATAACTGCGCAACCAGATAtt                                        <  2:488133/148‑1 (MQ=255)
                                                                                                      cAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATAtt                                        <  2:488273/148‑1 (MQ=255)
                                                                                                       aaTATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTa                                       >  2:441211/1‑148 (MQ=255)
                                                                                                       aaTATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTa                                       >  1:1264523/1‑148 (MQ=255)
                                                                                                         tataCAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTAcc                                     <  2:155271/148‑1 (MQ=255)
                                                                                                          ataCAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCACAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCg                                    >  2:3204892/1‑148 (MQ=255)
                                                                                                          ataCAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCg                                    >  1:328961/1‑148 (MQ=255)
                                                                                                           taCAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGACAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGt                                   <  2:2113403/148‑1 (MQ=255)
                                                                                                            aCAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTc                                  >  2:260205/1‑148 (MQ=255)
                                                                                                             cAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCt                                 <  2:432269/148‑1 (MQ=255)
                                                                                                              aGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCtt                                >  1:3255847/1‑148 (MQ=255)
                                                                                                               gACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCttt                               <  2:3064711/148‑1 (MQ=255)
                                                                                                               gACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCttt                               >  1:3820318/1‑148 (MQ=255)
                                                                                                               gACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCttt                               >  1:2219501/1‑148 (MQ=255)
                                                                                                               gACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCACTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCttt                               <  1:2575946/148‑1 (MQ=255)
                                                                                                                  cGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTAct                            <  1:3154292/148‑1 (MQ=255)
                                                                                                                   gACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTActc                           <  2:2969571/148‑1 (MQ=255)
                                                                                                                      aTACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGa                        <  1:3690094/148‑1 (MQ=255)
                                                                                                                       tACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGAt                       <  2:1387358/148‑1 (MQ=255)
                                                                                                                       tACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGAt                       >  2:738976/1‑148 (MQ=255)
                                                                                                                       tACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGAt                       <  1:3088271/148‑1 (MQ=255)
                                                                                                                            cGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGc                  >  1:2862580/1‑148 (MQ=255)
                                                                                                                                 ttACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTc             >  1:456315/1‑148 (MQ=255)
                                                                                                                                  tACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCa            >  1:11287/1‑148 (MQ=255)
                                                                                                                                   aCATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCAc           >  1:3391346/1‑148 (MQ=255)
                                                                                                                                    cATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCAcc          <  1:2644861/148‑1 (MQ=255)
                                                                                                                                     aTCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCAccc         <  1:1174531/148‑1 (MQ=255)
                                                                                                                                      tCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCa        >  2:1731752/1‑148 (MQ=255)
                                                                                                                                      tCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCa        >  1:3085447/1‑148 (MQ=255)
                                                                                                                                       cAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCAt       <  1:3431912/148‑1 (MQ=255)
                                                                                                                                       cAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCAt       <  1:1532508/148‑1 (MQ=255)
                                                                                                                                            agaCTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCATCCGtt  >  2:3741754/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                               
TGTAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCATCCGTT  >  CP009273/1249083‑1249370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCGCTGTAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCATCCGTTGCATCA  >  CP009273/1249078‑1249376
                                                                                                                                                     |                                                                                                                                                     
ATCGCTGTAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACT                                                                                                                                                       >  SRR19973069.3790555/1‑150 (MQ=60)
  CGCTGTAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTT                                                                                                                                                     <  SRR19973069.3528537/150‑1 (MQ=60)
  CGCTGTAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTT                                                                                                                                                     <  SRR19973069.1620425/150‑1 (MQ=60)
   GCTGTAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTT                                                                                                                                                    <  SRR19973069.1501426/150‑1 (MQ=60)
     TGTAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTAT                                                                                                                                                  <  SRR19973069.2170389/150‑1 (MQ=60)
      GTAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATC                                                                                                                                                 <  SRR19973069.3518270/150‑1 (MQ=60)
       TAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCC                                                                                                                                                <  SRR19973069.1757254/150‑1 (MQ=60)
       TAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCC                                                                                                                                                <  SRR19973069.900622/150‑1 (MQ=60)
       TAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCC                                                                                                                                                <  SRR19973069.2058784/150‑1 (MQ=60)
       TAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCC                                                                                                                                                <  SRR19973069.1757265/150‑1 (MQ=60)
        AAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCC                                                                                                                                               <  SRR19973069.397689/150‑1 (MQ=60)
         AAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCC                                                                                                                                              <  SRR19973069.139961/150‑1 (MQ=60)
              CGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAA                                                                                                                                         >  SRR19973069.435445/1‑150 (MQ=60)
                TTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAAC                                                                                                                                       <  SRR19973069.1193218/150‑1 (MQ=60)
                TTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAAC                                                                                                                                       <  SRR19973069.1193573/150‑1 (MQ=60)
                 TTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACT                                                                                                                                      <  SRR19973069.1186063/150‑1 (MQ=60)
                  TTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTG                                                                                                                                     <  SRR19973069.1158059/150‑1 (MQ=60)
                    GTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGA                                                                                                                                   <  SRR19973069.594736/150‑1 (MQ=60)
                     TGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGAT                                                                                                                                  >  SRR19973069.2946527/1‑150 (MQ=60)
                     TGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGAT                                                                                                                                  >  SRR19973069.2946520/1‑150 (MQ=60)
                     TGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGAT                                                                                                                                  <  SRR19973069.2039357/150‑1 (MQ=60)
                       CCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATAC                                                                                                                                <  SRR19973069.2124006/150‑1 (MQ=60)
                           GTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCT                                                                                                                            <  SRR19973069.2978038/150‑1 (MQ=60)
                           GTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCT                                                                                                                            <  SRR19973069.1897562/150‑1 (MQ=60)
                            TAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTG                                                                                                                           >  SRR19973069.987318/1‑150 (MQ=60)
                             AACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGT                                                                                                                          >  SRR19973069.491654/1‑150 (MQ=60)
                             AACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGT                                                                                                                          >  SRR19973069.799925/1‑150 (MQ=60)
                             AACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGT                                                                                                                          >  SRR19973069.491796/1‑150 (MQ=60)
                                TGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAAC                                                                                                                       <  SRR19973069.1816988/150‑1 (MQ=60)
                                    GTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCAT                                                                                                                   <  SRR19973069.950563/150‑1 (MQ=60)
                                     TACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATG                                                                                                                  <  SRR19973069.1463400/150‑1 (MQ=60)
                                     TACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATG                                                                                                                  <  SRR19973069.3377452/150‑1 (MQ=60)
                                     TACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATG                                                                                                                  <  SRR19973069.498637/150‑1 (MQ=60)
                                        ATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCA                                                                                                               >  SRR19973069.1601805/1‑150 (MQ=60)
                                        ATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCA                                                                                                               >  SRR19973069.1339825/1‑150 (MQ=60)
                                           CCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAG                                                                                                            <  SRR19973069.3247851/150‑1 (MQ=60)
                                                  AGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCA                                                                                                     >  SRR19973069.3233329/1‑150 (MQ=60)
                                                     CTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTT                                                                                                  >  SRR19973069.642294/1‑150 (MQ=60)
                                                     CTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTT                                                                                                  <  SRR19973069.957971/150‑1 (MQ=60)
                                                      TGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTT                                                                                                 <  SRR19973069.173177/150‑1 (MQ=60)
                                                      TGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTT                                                                                                 <  SRR19973069.3370197/150‑1 (MQ=60)
                                                      TGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTT                                                                                                 <  SRR19973069.3284182/150‑1 (MQ=60)
                                                        AACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGC                                                                                               >  SRR19973069.156637/1‑150 (MQ=60)
                                                            TGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACT                                                                                           <  SRR19973069.1022848/150‑1 (MQ=60)
                                                                   CCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAG                                                                                    <  SRR19973069.1396748/150‑1 (MQ=60)
                                                                   CCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCCACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAG                                                                                    <  SRR19973069.1397266/150‑1 (MQ=60)
                                                                    CGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGG                                                                                   <  SRR19973069.711417/150‑1 (MQ=60)
                                                                    CGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGG                                                                                   <  SRR19973069.714907/150‑1 (MQ=60)
                                                                    CGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGG                                                                                   <  SRR19973069.711444/150‑1 (MQ=60)
                                                                     GTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGA                                                                                  >  SRR19973069.3450316/1‑150 (MQ=60)
                                                                     GTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGA                                                                                  >  SRR19973069.2700539/1‑150 (MQ=60)
                                                                         CCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCC                                                                              >  SRR19973069.2326264/1‑150 (MQ=60)
                                                                          CTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCA                                                                             <  SRR19973069.2170932/150‑1 (MQ=60)
                                                                          CTCCGCTATAGTCCGGCGATGCATGTGTTGAGCAAATATACAGACCGACATACAACGGAATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCA                                                                             <  SRR19973069.2170953/150‑1 (MQ=60)
                                                                              GCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCT                                                                         <  SRR19973069.2108482/150‑1 (MQ=60)
                                                                               CTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTT                                                                        <  SRR19973069.1501100/150‑1 (MQ=60)
                                                                               CTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTT                                                                        <  SRR19973069.2623823/150‑1 (MQ=60)
                                                                                  TAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCC                                                                     >  SRR19973069.3064319/1‑150 (MQ=60)
                                                                                  TAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCC                                                                     <  SRR19973069.3795268/150‑1 (MQ=60)
                                                                                         GCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGAATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAAC                                                              <  SRR19973069.2485928/150‑1 (MQ=60)
                                                                                             TGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCG                                                          >  SRR19973069.306509/1‑150 (MQ=60)
                                                                                             TGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCG                                                          >  SRR19973069.306557/1‑150 (MQ=60)
                                                                                               CATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCA                                                        <  SRR19973069.3278308/150‑1 (MQ=60)
                                                                                                      TGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGAT                                                 <  SRR19973069.1174482/150‑1 (MQ=60)
                                                                                                      TGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGAT                                                 <  SRR19973069.1174160/150‑1 (MQ=60)
                                                                                                       GAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATA                                                <  SRR19973069.3484545/150‑1 (MQ=60)
                                                                                                       GAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATA                                                <  SRR19973069.3301385/150‑1 (MQ=60)
                                                                                                          CCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTA                                             <  SRR19973069.1069754/150‑1 (MQ=60)
                                                                                                          CCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTA                                             <  SRR19973069.1070058/150‑1 (MQ=60)
                                                                                                          CCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTA                                             >  SRR19973069.1273038/1‑150 (MQ=60)
                                                                                                          CCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTA                                             <  SRR19973069.2386552/150‑1 (MQ=60)
                                                                                                           CAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTAC                                            >  SRR19973069.1380041/1‑150 (MQ=60)
                                                                                                           CAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTAC                                            <  SRR19973069.1442957/150‑1 (MQ=60)
                                                                                                             ATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCG                                          >  SRR19973069.331199/1‑150 (MQ=60)
                                                                                                                TACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCT                                       >  SRR19973069.2464673/1‑150 (MQ=60)
                                                                                                                 ACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTT                                      >  SRR19973069.3277154/1‑150 (MQ=60)
                                                                                                                 ACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTT                                      >  SRR19973069.3277168/1‑150 (MQ=60)
                                                                                                                  CAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTT                                     >  SRR19973069.2234514/1‑150 (MQ=60)
                                                                                                                  CAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTT                                     >  SRR19973069.3845034/1‑150 (MQ=60)
                                                                                                                  CAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTT                                     >  SRR19973069.2234517/1‑150 (MQ=60)
                                                                                                                    GACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCACTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTAC                                   <  SRR19973069.2593751/150‑1 (MQ=60)
                                                                                                                    GACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCACTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTAC                                   <  SRR19973069.2593042/150‑1 (MQ=60)
                                                                                                                       CGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCT                                <  SRR19973069.3174895/150‑1 (MQ=60)
                                                                                                                       CGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCT                                <  SRR19973069.3173941/150‑1 (MQ=60)
                                                                                                                       CGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCT                                <  SRR19973069.2589919/150‑1 (MQ=60)
                                                                                                                          CATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGAT                             <  SRR19973069.3089514/150‑1 (MQ=60)
                                                                                                                           ATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATT                            <  SRR19973069.3714227/150‑1 (MQ=60)
                                                                                                                            TACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTT                           <  SRR19973069.3108634/150‑1 (MQ=60)
                                                                                                                               AACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGC                        >  SRR19973069.2881397/1‑150 (MQ=60)
                                                                                                                               AACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGC                        >  SRR19973069.2274193/1‑150 (MQ=60)
                                                                                                                                    CATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTC                   >  SRR19973069.460629/1‑150 (MQ=60)
                                                                                                                                    CATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTC                   >  SRR19973069.459658/1‑150 (MQ=60)
                                                                                                                                     ATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCA                  >  SRR19973069.11448/1‑150 (MQ=60)
                                                                                                                                      TTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCAC                 >  SRR19973069.3413495/1‑150 (MQ=60)
                                                                                                                                         CATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCA              >  SRR19973069.3105792/1‑150 (MQ=60)
                                                                                                                                         CATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCA              <  SRR19973069.2662577/150‑1 (MQ=60)
                                                                                                                                          ATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCAT             <  SRR19973069.1182665/150‑1 (MQ=60)
                                                                                                                                            CAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCATCC           <  SRR19973069.2350149/150‑1 (MQ=60)
                                                                                                                                            CAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCATCC           <  SRR19973069.1543037/150‑1 (MQ=60)
                                                                                                                                            CAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCATCC           <  SRR19973069.3602991/150‑1 (MQ=60)
                                                                                                                                            CAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCATCC           <  SRR19973069.3454218/150‑1 (MQ=60)
                                                                                                                                                     TTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCATCCGTTGCATCA  <  SRR19973069.2812490/150‑1 (MQ=60)
                                                                                                                                                     TTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCATCCGTTGCATCA  <  SRR19973069.2812520/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
ATCGCTGTAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCATCCGTTGCATCA  >  CP009273/1249078‑1249376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑