Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,221,214 C→A 44.1% intergenic (+60/+73) rluF → / ← yjbD 23S rRNA pseudouridine(2604) synthase/DUF3811 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,221,2140CA44.1% 245.3 / 297.8 269intergenic (+60/+73)rluF/yjbD23S rRNA pseudouridine(2604) synthase/DUF3811 family protein
Reads supporting (aligned to +/- strand):  ref base C (66/84);  new base A (62/57);  total (128/141)
Fisher's exact test for biased strand distribution p-value = 2.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 272 total aligned reads displayed.

GATGGAAAAAACGGCGGAAAAAGGCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGACCAGCTAAATGACGCTTCAGGATCCGGTTTATAAGCCTGCT  >  CP009273/4221067‑4221360
                                                                                                                                                   |                                                                                                                                                  
gATGCAAAAAACGGCGCAAAAAGGCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTc                                                                                                                                                    <  1:2060472/148‑1 (MQ=255)
 aTGGAAAAAACGGCGGAAAAAGGCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCg                                                                                                                                                   <  1:1863387/148‑1 (MQ=255)
        aaaCGGCGGAAAAAGGCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAg                                                                                                                                            <  1:2513538/148‑1 (MQ=255)
        aaaCGGCGGAAAAAGGCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAg                                                                                                                                            <  2:767716/148‑1 (MQ=255)
        aaaCGGCGGAAAAAGGCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAg                                                                                                                                            <  2:1492514/148‑1 (MQ=255)
        aaaCGGCGGAAAAAGGCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAg                                                                                                                                            >  2:1983629/1‑148 (MQ=255)
         aaCGGCGGAAAAAGGCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAACGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAgg                                                                                                                                           <  2:2417001/148‑1 (MQ=255)
          aCGGCGGAAAAAGGCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAggg                                                                                                                                          >  2:1526646/1‑148 (MQ=255)
          aCGGCGGAAAAAGGCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAggg                                                                                                                                          <  2:2737072/148‑1 (MQ=255)
             gcggAAAAAGGCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTa                                                                                                                                       <  1:3026949/148‑1 (MQ=255)
             gcggAAAAAGGCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTa                                                                                                                                       <  1:1475368/148‑1 (MQ=255)
                gAAAAAGGCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAggg                                                                                                                                    >  1:2214166/1‑148 (MQ=255)
                   aaaGGCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCGTAGGGAAAAGGGGTAGGGTGa                                                                                                                                 >  1:971669/1‑148 (MQ=255)
                    aaGGCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAg                                                                                                                                >  2:825659/1‑148 (MQ=255)
                    aaGGCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAg                                                                                                                                >  1:2347611/1‑148 (MQ=255)
                      ggCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCACTCGCCCCTTAGGGCAAACGGTTAGGGTGAggg                                                                                                                              >  1:2878389/1‑148 (MQ=255)
                       gCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAgggg                                                                                                                             >  2:2459710/1‑148 (MQ=255)
                       gCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAgggg                                                                                                                             >  2:2459649/1‑148 (MQ=255)
                       gCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAgggg                                                                                                                             >  2:1188489/1‑148 (MQ=255)
                       gCGGCCGCCCGGCGACCAACGGTACGCGTTTTACCGCGCCGGGGCTAAAAACCACGGGGCGCTGATTAACGCCTCAGACTGATGAACACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAgggg                                                                                                                             <  1:1615528/148‑1 (MQ=37)
                          gTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTACCGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGaaa                                                                                                                          <  2:2796031/148‑1 (MQ=255)
                          gTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGaaa                                                                                                                          <  1:2690297/148‑1 (MQ=255)
                           tCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGaaaa                                                                                                                         <  1:3159477/148‑1 (MQ=255)
                           tCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGaaaa                                                                                                                         <  2:2847435/148‑1 (MQ=255)
                              cccGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATtg                                                                                                                      >  2:282113/1‑148 (MQ=255)
                                cGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATtgtg                                                                                                                    <  2:3733300/148‑1 (MQ=255)
                                cGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATtgtg                                                                                                                    <  1:3539446/148‑1 (MQ=255)
                                        aaCGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACt                                                                                                            >  2:251650/1‑148 (MQ=255)
                                          cGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAACAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGa                                                                                                          >  2:3163931/1‑148 (MQ=255)
                                          cGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGa                                                                                                          <  2:2371487/148‑1 (MQ=255)
                                              aaGCGTTGTACCTCGCC‑GGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGt                                                                                                     <  2:3057335/148‑1 (MQ=255)
                                                gCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTg                                                                                                    >  1:1009072/1‑148 (MQ=255)
                                                         cTCGCCGGTGAGTAAAAAGAAGGGGCTCTTTTTAAGGCCTCAGACTGATGAAAACTGCCCAATAGTGCCCGGACAGTCCCCTGGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCTCTCTGACGAGTGAAAATCTCa                                                                                           <  2:2990002/148‑1 (MQ=37)
                                                          tCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAg                                                                                          <  2:1640238/148‑1 (MQ=255)
                                                           cGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAgg                                                                                         <  1:3703384/148‑1 (MQ=255)
                                                              cGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGt                                                                                      <  2:1396425/148‑1 (MQ=255)
                                                                 ggCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGa                                                                                   <  1:2366608/148‑1 (MQ=255)
                                                                  gCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGAt                                                                                  <  1:3392817/148‑1 (MQ=255)
                                                                  gCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGAt                                                                                  <  1:2425096/148‑1 (MQ=255)
                                                                  gCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGAt                                                                                  >  2:1690872/1‑148 (MQ=255)
                                                                   cGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGAtt                                                                                 <  2:1647062/148‑1 (MQ=255)
                                                                     tAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATtgt                                                                               >  2:1802896/1‑148 (MQ=255)
                                                                      aaaaaGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATtgtg                                                                              >  2:1290880/1‑148 (MQ=255)
                                                                        aaaGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGa                                                                            <  2:1915245/148‑1 (MQ=255)
                                                                          agaagGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGAtt                                                                          <  2:3730926/148‑1 (MQ=255)
                                                                           gaagGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTa                                                                         >  2:1928774/1‑148 (MQ=255)
                                                                           gaagGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTa                                                                         >  2:3008327/1‑148 (MQ=255)
                                                                            aagGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAg                                                                        >  1:3647136/1‑148 (MQ=255)
                                                                              ggggCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGACAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAgcg                                                                      >  2:2950230/1‑148 (MQ=255)
                                                                              ggggCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAgcg                                                                      <  2:2565554/148‑1 (MQ=255)
                                                                                ggCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAACAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGcc                                                                    <  2:3748197/148‑1 (MQ=255)
                                                                                  cgcTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTg                                                                  <  1:2537165/148‑1 (MQ=255)
                                                                                          aaCGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGt                                                          <  2:679324/148‑1 (MQ=255)
                                                                                           aCGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTc                                                         >  1:1983237/1‑148 (MQ=255)
                                                                                            cGCCTCAGACTGATGAACACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGACAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGAGTAGCGCCTGCCGCGCGTCg                                                        >  1:1514534/1‑148 (MQ=255)
                                                                                            cGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCg                                                        <  2:2323867/148‑1 (MQ=255)
                                                                                              ccTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGAc                                                      >  2:2265207/1‑148 (MQ=255)
                                                                                                 cAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTa                                                   <  1:1003615/148‑1 (MQ=255)
                                                                                                  aGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTAt                                                  <  1:3286555/148‑1 (MQ=255)
                                                                                                  aGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTAt                                                  <  1:3416694/148‑1 (MQ=255)
                                                                                                  aGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTAt                                                  <  1:3127637/148‑1 (MQ=255)
                                                                                                    aCTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTAttt                                                <  1:181723/148‑1 (MQ=255)
                                                                                                     cTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTg                                               <  2:3440931/148‑1 (MQ=255)
                                                                                                      tgatgaAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGc                                              <  2:3053935/148‑1 (MQ=255)
                                                                                                      tgatgaAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGc                                              >  1:2271469/1‑148 (MQ=255)
                                                                                                        atgaAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGACAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTg                                            >  2:3310583/1‑148 (MQ=255)
                                                                                                        atgaAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTg                                            >  1:2007711/1‑148 (MQ=255)
                                                                                                        atgaAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTg                                            >  1:3756291/1‑148 (MQ=255)
                                                                                                        atgaAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCATTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTATCGCCTGCCGCGCGTCGACGTATATGCTg                                            >  1:436117/1‑148 (MQ=255)
                                                                                                          gaAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGAc                                          >  2:2176570/1‑148 (MQ=255)
                                                                                                           aaaaCGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGAcc                                         >  1:1898161/1‑148 (MQ=255)
                                                                                                             aaCGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGACCAg                                       <  1:638781/148‑1 (MQ=255)
                                                                                                              aCGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGACCAGc                                      <  2:1304943/148‑1 (MQ=255)
                                                                                                               cGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGACCAGCt                                     >  2:3606463/1‑148 (MQ=255)
                                                                                                                      aaaGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGACCAGCTAAATGAc                              >  2:1844068/1‑148 (MQ=255)
                                                                                                                       aaGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGACCAGCTAAATGACg                             <  1:1105133/148‑1 (MQ=255)
                                                                                                                       aaGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGACCAGCTAAATGACg                             <  2:1072441/148‑1 (MQ=255)
                                                                                                                        aGTGCCCGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGACCAGCTAAATGACGc                            >  1:664699/1‑148 (MQ=255)
                                                                                                                          tGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGACCAGCTAAATGACGCtt                          <  2:1135926/148‑1 (MQ=255)
                                                                                                                          tGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGACCAGCTAAATGACGCtt                          <  2:2644479/148‑1 (MQ=255)
                                                                                                                            cccGGACAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGACCAGCTAAATGACGCTTCa                        <  1:3337079/148‑1 (MQ=255)
                                                                                                                                gACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGACCAGCTAAATGACGCTTCAGGAt                    <  1:178762/148‑1 (MQ=255)
                                                                                                                                 aCAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGACCAGCTAAATGACGCTTCAGGATc                   >  1:678679/1‑148 (MQ=255)
                                                                                                                                 aCAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGACCAGCTAAATGACGCTTCAGGATc                   >  2:1722920/1‑148 (MQ=255)
                                                                                                                                 aCAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGACCAGCTAAATGACGCTTCAGGATc                   >  1:2598384/1‑148 (MQ=255)
                                                                                                                                 aCAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGACCAGCTAAATGACGCTTCAGGATc                   >  2:2315055/1‑148 (MQ=255)
                                                                                                                                 aCAGTCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGACCAGCTAAATGACGCTTCAGGATc                   <  2:479715/148‑1 (MQ=255)
                                                                                                                                     tCCCCTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGACCAGCTAAATGACGCTTCAGGATCCGGt               <  2:1008967/148‑1 (MQ=255)
                                                                                                                                      ccccTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGACCAGCTAAATGACGCTTCAGGATCCGGtt              <  2:2207558/148‑1 (MQ=255)
                                                                                                                                      ccccTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGACCAGCTAAATGACGCTTCAGGATCCGGtt              <  2:2074198/148‑1 (MQ=255)
                                                                                                                                      ccccTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGACCAGCTAAATGACGCTTCAGGATCCGGtt              >  1:2633517/1‑148 (MQ=255)
                                                                                                                                       cccTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGACCAGCTAAATGACGCTTCAGGATCCGGttt             >  1:97826/1‑148 (MQ=255)
                                                                                                                                       cccTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGACCAGCTAAATGACGCTTCAGGATCCGGttt             <  1:2757816/148‑1 (MQ=255)
                                                                                                                                        ccTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGACCAGCTAAATGACGCTTCAGGATCCGGTTta            <  2:3131974/148‑1 (MQ=255)
                                                                                                                                        ccTCGCCCCTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGACCAGCTAAATGACGCTTCAGGATCCGGTTta            >  2:1918490/1‑148 (MQ=255)
                                                                                                                                          tCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGACCAGCTAAATGACGCTTCAGGATCCGGTTtata          <  2:75241/148‑1 (MQ=255)
                                                                                                                                              cccTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGACCAGCTAAATGACGCTTCAGGATCCGGTTTATAAGcc      <  2:3067846/148‑1 (MQ=255)
                                                                                                                                                cTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGACCAGCTAAATGACGCTTCAGGATCCGGTTTATAAGCCTg    <  1:1288589/148‑1 (MQ=255)
                                                                                                                                                cTTAGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGACCAGCTAAATGACGCTTCAGGATCCGGTTTATAAGCCTg    >  2:3370925/1‑148 (MQ=255)
                                                                                                                                                  tCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGACCAGCTAAATGACGCTTCAGGATCCGGTTTATAAGCCTGCt  <  1:3008327/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GATGGAAAAAACGGCGGAAAAAGGCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGATTAGCGCCTGCCGCGCGTCGACGTATTTGCTGACCAGCTAAATGACGCTTCAGGATCCGGTTTATAAGCCTGCT  >  CP009273/4221067‑4221360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A