Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F55 I0 R1 519 344.2 11360120 95.0% 10792114 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,539,484 G→A 18.8% pseudogene (488/951 nt) yddK ← pseudogene, leucine‑rich protein; putative glycoportein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,539,4840GA18.8% 832.2 / 174.4 336pseudogene (488/951 nt)yddKpseudogene, leucine‑rich protein; putative glycoportein
Reads supporting (aligned to +/- strand):  ref base G (140/132);  new base A (32/31);  total (172/164)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.59e-01

Only 100 of 348 total aligned reads displayed.

TACCTGCAACAATATCAGTTAATTGATTATGGCTGAGATTCAGGTATTGCAGCCATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCGTAGTTGGTTGCATCAAAATGTGTGAATTGGTTATTATCAAGATT  >  CP009273/1539335‑1539632
                                                                                                                                                     |                                                                                                                                                    
taCCTGCAACAATATCAGTTAATTGATTATGGCTGAGATTCAGGTATTGCAGCCATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGagag                                                                                                                                                      >  2:4331977/1‑150 (MQ=255)
taCCTGCAACAATATCAGTTAATTGATTATGGCTGAGATTCAGGTATTGCAGCCATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGagag                                                                                                                                                      >  1:32478/1‑150 (MQ=255)
taCCTGCAACAATATCAGTTAATTGATTATGGCTGAGATTCAGGTATTGCAGCCATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGagag                                                                                                                                                      >  1:2907412/1‑150 (MQ=255)
   cTGCAACAATATCAGTTAATTGATTATGGCTGAGATTCAGGTATTGCAGCCATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCat                                                                                                                                                   <  2:4626351/150‑1 (MQ=255)
   cTGCAACAATATCAGTTAATTGATTATGGCTGAGATTCAGGTATTGCAGCCATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGAat                                                                                                                                                   >  2:2828913/1‑150 (MQ=255)
    tGCAACAATATCAGTTAATTGATTATGGCTGAGATTCAGGTATTGCAGCCATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCatt                                                                                                                                                  >  2:3378931/1‑150 (MQ=255)
     gCAACAATATCAGTTAATTGATTATGGCTGAGATTCAGGTATTGCAGCCATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAACatta                                                                                                                                                 <  2:3109947/150‑1 (MQ=255)
      caacaaTATCAGTTAATTGATTATGGCTGAGATTCAGGTATTGCAGCCATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCattat                                                                                                                                                >  2:1595992/1‑150 (MQ=255)
      caacaaTATCAGTTAATTGATTATGGCTGAGATTCAGGTATTGCAGCCATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCattat                                                                                                                                                >  1:4934854/1‑150 (MQ=255)
         caaTATCAGTTAATTGATTATGGCTGAGATTCAGGTATTGCAGCCATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCattattat                                                                                                                                             <  2:4057013/150‑1 (MQ=255)
                   tAATTGATTATGGCTGAGATTCAGGTATTGCAGCCATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGatat                                                                                                                                   <  1:523458/150‑1 (MQ=255)
                   tAATTGATTATGGCTGAGATTCAGGTATTGCAGCCATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGatat                                                                                                                                   <  2:4263464/150‑1 (MQ=255)
                             tGGCTGAGATTCAGGTATTGCAGCCATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATa                                                                                                                         <  2:3504673/150‑1 (MQ=255)
                              ggCTGAGATTCAGGTATTGCAGCCATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAACATTATTATTCATAGATATATGTGTAATAc                                                                                                                        >  2:2386991/1‑150 (MQ=255)
                               gCTGAGATTCAGGTATTGCAGCCATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACt                                                                                                                       <  1:1013074/150‑1 (MQ=255)
                               gCTGAGATTCAGGTATTGCAGCCATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACt                                                                                                                       <  1:1714710/150‑1 (MQ=255)
                                  gagaTTCAGGTATTGCAGCCATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTAc                                                                                                                    <  2:5225394/150‑1 (MQ=255)
                                    gaTTCAGGTATTGCAGCCATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACAt                                                                                                                  <  2:683157/150‑1 (MQ=255)
                                     aTTCAGGTATTGCAGCCATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACAtt                                                                                                                 <  2:2283718/150‑1 (MQ=255)
                                      ttCAGGTATTGCAGCCATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACAttt                                                                                                                <  2:571516/150‑1 (MQ=255)
                                           gTATTGCAGCCATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGcc                                                                                                           <  2:2558044/150‑1 (MQ=255)
                                           gTATTGCAGCCATTCGCAAGATTCTAAAAGCACAAACACTAGTTGAATATGTGCCTCACTAAAATAACTAACTGATGAAAGCCTATCAATATCAATATTTCTTATAGAATTATTATACATATATATATGTGTAATAATTACAtttattca                                                                                                           >  2:2323139/1‑145 (MQ=255)
                                            tATTGCAGCCATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCt                                                                                                          <  2:3175075/150‑1 (MQ=255)
                                              ttGCATCCATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATTTGCCTCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTg                                                                                                        <  1:3610291/150‑1 (MQ=255)
                                              ttGCAGCCATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTg                                                                                                        <  1:2586791/150‑1 (MQ=255)
                                               tGCAGCCATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGa                                                                                                       <  2:5419986/150‑1 (MQ=255)
                                                gCAGCCATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGATAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAg                                                                                                      <  2:916769/150‑1 (MQ=255)
                                                 cAGCCATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAACATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAgg                                                                                                     <  1:614444/150‑1 (MQ=255)
                                                   gCCATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATCTTTCTGAGAACATTATTATTCATAGATCTATGTGTAATACTTACATTTCTGCCTTGAGGaa                                                                                                   >  2:3954808/1‑150 (MQ=255)
                                                    ccATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAACATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGaaa                                                                                                  <  2:3234244/150‑1 (MQ=255)
                                                     cATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGCTGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATATATATATGTGTAATACTTACATTTCTGCCTTGAGGaaaa                                                                                                 >  1:4121063/1‑150 (MQ=255)
                                                     cATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGaaaa                                                                                                 <  1:88784/150‑1 (MQ=255)
                                                       ttCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAAtt                                                                                               <  2:782065/150‑1 (MQ=255)
                                                       ttCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAAtt                                                                                               >  2:1376659/1‑150 (MQ=255)
                                                       ttCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAAtt                                                                                               >  1:93176/1‑150 (MQ=255)
                                                       ttCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAACATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAAtt                                                                                               <  2:5029513/150‑1 (MQ=255)
                                                         cGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTa                                                                                             <  1:4566148/150‑1 (MQ=255)
                                                          gCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAAGTTAt                                                                                            >  2:5287699/1‑150 (MQ=255)
                                                            aaGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTg                                                                                          >  1:5047768/1‑150 (MQ=255)
                                                              gATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAACATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGAc                                                                                        <  1:661724/150‑1 (MQ=255)
                                                               aTTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTTAGGACAATTTATTGTCt                                                                                       >  2:3061553/1‑150 (MQ=255)
                                                                  cTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCaa                                                                                    <  2:2525157/150‑1 (MQ=255)
                                                                  cTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCaa                                                                                    <  1:1616771/150‑1 (MQ=255)
                                                                   tAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAag                                                                                   <  1:4762752/150‑1 (MQ=255)
                                                                     aTTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAagag                                                                                 >  2:1933938/1‑150 (MQ=255)
                                                                          acaAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTa                                                                            >  1:3080271/1‑150 (MQ=255)
                                                                           caAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTAc                                                                           >  2:3865958/1‑150 (MQ=255)
                                                                            aaaCTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACt                                                                          <  2:5492970/150‑1 (MQ=255)
                                                                             aaCTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTa                                                                         <  2:2115352/150‑1 (MQ=255)
                                                                             aaCTATAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAACATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTa                                                                         <  2:5110907/150‑1 (MQ=255)
                                                                               ctctAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTAtt                                                                       <  2:4128256/150‑1 (MQ=255)
                                                                               ctctAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTAtt                                                                       >  2:5345641/1‑150 (MQ=255)
                                                                                   aGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTTACTCAAGAGCATTACTATTCata                                                                   >  2:3280273/1‑148 (MQ=255)
                                                                                   aGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAACATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGa                                                                   >  1:2151505/1‑150 (MQ=255)
                                                                                    gTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGAc                                                                  >  2:3663372/1‑150 (MQ=255)
                                                                                     ttGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACt                                                                 <  1:4403316/150‑1 (MQ=255)
                                                                                       gATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTa                                                               <  2:1442143/150‑1 (MQ=255)
                                                                                          tATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCt                                                            >  2:2175167/1‑150 (MQ=255)
                                                                                            tgtgCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCttt                                                          >  2:4821055/1‑150 (MQ=255)
                                                                                            tgtgCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCttt                                                          <  1:1083277/150‑1 (MQ=255)
                                                                                            tgtgCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCttt                                                          <  2:1726665/150‑1 (MQ=255)
                                                                                              tgCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTAc                                                        <  2:2753932/150‑1 (MQ=255)
                                                                                                 cGCACTAAAATAAGTAACTGAAGAAAGCCTCTCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAg                                                     <  1:2889958/150‑1 (MQ=255)
                                                                                                   cACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTc                                                   >  2:1995080/1‑150 (MQ=255)
                                                                                                    aCTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCt                                                  >  2:2414464/1‑150 (MQ=255)
                                                                                                     cTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAACATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTa                                                 <  2:4418407/150‑1 (MQ=255)
                                                                                                      tAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTAt                                                >  2:5174524/1‑150 (MQ=255)
                                                                                                        aaaTAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAACATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCg                                              >  2:1837945/1‑150 (MQ=255)
                                                                                                         aaTAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCGt                                             >  2:1310204/1‑150 (MQ=255)
                                                                                                         aaTAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCGt                                             <  2:1190220/150‑1 (MQ=255)
                                                                                                           tAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCGTAg                                           >  1:1212520/1‑150 (MQ=255)
                                                                                                             aGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCGTAgtt                                         >  1:4862319/1‑150 (MQ=255)
                                                                                                               tAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCGTAgttgg                                       >  2:3807736/1‑150 (MQ=255)
                                                                                                               tAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCGTAgttgg                                       >  2:1546501/1‑150 (MQ=255)
                                                                                                                 aCTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCGTAgttggtt                                     <  1:4007627/150‑1 (MQ=255)
                                                                                                                  cTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCGTAgttggttg                                    <  1:694829/150‑1 (MQ=255)
                                                                                                                   tgatgaAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCGTAGTTGGTTGc                                   <  2:1009839/150‑1 (MQ=255)
                                                                                                                        aaaGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCGTAGTTGGTTGCATCaa                              >  2:1201337/1‑150 (MQ=255)
                                                                                                                        aaaGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCGTAGTTGGTTGCATCaa                              >  1:251352/1‑150 (MQ=255)
                                                                                                                         aaGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCGTAGTTGGTTGCATCaaa                             >  1:1311168/1‑150 (MQ=255)
                                                                                                                           gCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCGTAGTTGGTTGCATCAAAAt                           >  2:867726/1‑150 (MQ=255)
                                                                                                                           gCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCGTAGTTGGTTGCATCAAAAt                           <  1:2043402/150‑1 (MQ=255)
                                                                                                                             ctatctatATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCGTAGTTGGTTGCATCAAAAtgt                         <  1:5173429/150‑1 (MQ=255)
                                                                                                                                tctatATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCGTAGTTGGTTGCATCAAAAtgtgtg                      >  1:812581/1‑150 (MQ=255)
                                                                                                                                  tataTCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCGTAGTTGGTTGCATCAAAATGTGTGaa                    <  1:3857221/150‑1 (MQ=255)
                                                                                                                                    tatCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCGTAGTTGGTTGCATCAAAATGTGTGAAtt                  <  1:1450723/150‑1 (MQ=255)
                                                                                                                                      tCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCGTAGTTGGTTGCATCAAAATGTGTGAATTgg                >  1:3398843/1‑150 (MQ=255)
                                                                                                                                      tCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCGTAGTTGGTTGCATCAAAATGTGTGAATTgg                <  1:5413485/150‑1 (MQ=255)
                                                                                                                                        aaTATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCGTAGTTGGTTGCATCAAAATGTGTGAATTGGtt              >  2:2695095/1‑150 (MQ=255)
                                                                                                                                           atTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCGTAGTTGGTTGCATCAAAATGTGTGAATTGGttatt           >  2:498213/1‑150 (MQ=255)
                                                                                                                                           atTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCGTAGTTGGTTGCATCAAAATGTGTGAATTGGttatt           <  2:4646540/150‑1 (MQ=255)
                                                                                                                                            tttCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCGTAGTTGGTTGCATCAAAATGTGTGAATTGGttatta          <  1:4331977/150‑1 (MQ=255)
                                                                                                                                               cTGAGATCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCTAGAGCATTACTATTCAGTCTTAGCTTTACGAGTCTTTCGTAGTTGGTTGCATCAAAATGTGTGAATTGGTTATTATCa       <  1:373141/150‑1 (MQ=255)
                                                                                                                                               cTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCGTAGTTGGTTGCATCAAAATGTGTGAATTGGTTATTATCa       <  2:346928/150‑1 (MQ=255)
                                                                                                                                               cTGAGAACATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCGTAGTTGGTTGCATCAAAATGTGTGAATTGGTTATTATCa       >  2:749603/1‑150 (MQ=255)
                                                                                                                                                tGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCGTAGTTGGTTGCATCAAAATGTGTGAATTGGTTATTATCaa      >  2:1061014/1‑150 (MQ=255)
                                                                                                                                                tGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCGTAGTTGGTTGCATCAAAATGTGTGAATTGGTTATTATCaa      >  1:256162/1‑150 (MQ=255)
                                                                                                                                                 gagaACATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCGTAGTTGGTTGCATCAAAATGTGTGAATTGGTTATTATCAAg     >  1:5577463/1‑150 (MQ=255)
                                                                                                                                                    agCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCGTAGTTGGTTGCATCAAAATGTGTGAATTGGTTATTATCAAGAtt  >  1:2297752/1‑150 (MQ=255)
                                                                                                                                                    agCATTATTATTCATAGAGATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACAATTCAGACTTAGCTTTACGAGTCTATCGTAGTTGGTTGCATCAAAATGTGTGAATTGGTTATTATCAAGAtt  >  2:2626352/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TACCTGCAACAATATCAGTTAATTGATTATGGCTGAGATTCAGGTATTGCAGCCATTCGCAAGATTCTAATTGCACAAACTCTAGTTGATTATGTGCCGCACTAAAATAAGTAACTGATGAAAGCCTATCTATATCAATATTTCTGAGAGCATTATTATTCATAGATATATGTGTAATACTTACATTTCTGCCTTGAGGAAAATTTATTGACTCAAGAGCATTACTATTCAGACTTAGCTTTACGAGTCTATCGTAGTTGGTTGCATCAAAATGTGTGAATTGGTTATTATCAAGATT  >  CP009273/1539335‑1539632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A