Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 173,725 G→T 100% G129G (GGC→GGA)  yadS ← UPF0126 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273173,7250GT99.3% 893.4 / ‑3.8 276G129G (GGC→GGA) yadSUPF0126 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (128/144);  total (131/145)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 280 total aligned reads displayed.

CATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCGCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCT  >  CP009273/173578‑173870
                                                                                                                                                   |                                                                                                                                                 
catcatACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCgcctcc                                                                                                                                                 >  2:1530957/1‑150 (MQ=255)
 atcatACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCa                                                                                                                                                <  2:2292178/150‑1 (MQ=255)
      aCTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGcc                                                                                                                                           <  2:112158/150‑1 (MQ=255)
       cTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCa                                                                                                                                          >  1:514626/1‑150 (MQ=255)
       cTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCa                                                                                                                                          <  1:2012464/150‑1 (MQ=255)
        tGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAg                                                                                                                                         <  2:2065587/150‑1 (MQ=255)
         ggCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGt                                                                                                                                        >  2:2920934/1‑150 (MQ=255)
          gCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTa                                                                                                                                       <  1:1300615/150‑1 (MQ=255)
          gCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTa                                                                                                                                       >  2:743930/1‑150 (MQ=255)
            tGTTTCCTGTGGTACGGAAAATGTGTAATAAGCCGTAGAGTGGACTATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAATATCTTGGGTATTTCGCTGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTatt                                                                                                                                     <  2:522881/150‑3 (MQ=255)
            tGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAAt                                                                                                                                     <  1:1958089/150‑1 (MQ=255)
            tGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAAt                                                                                                                                     >  2:3415375/1‑150 (MQ=255)
             gTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAGTGATCCCGCCTCCAACGCCAGTAATg                                                                                                                                    <  2:3037621/150‑1 (MQ=255)
                   aGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGccc                                                                                                                              >  2:2866995/1‑150 (MQ=255)
                    gTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACCGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCa                                                                                                                             <  1:328257/150‑1 (MQ=255)
                       gTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATAc                                                                                                                          <  1:2300348/150‑1 (MQ=255)
                              aaaTGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACcgc                                                                                                                   >  2:2278533/1‑150 (MQ=255)
                               aaTGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACcgcg                                                                                                                  >  1:4403376/1‑150 (MQ=255)
                               aaTGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACcgcg                                                                                                                  >  1:245393/1‑150 (MQ=255)
                                 tgtgTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGAt                                                                                                                <  2:83300/150‑1 (MQ=255)
                                  gtgtAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGAtt                                                                                                               >  1:4122516/1‑150 (MQ=255)
                                  gtgtAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGAtt                                                                                                               <  2:1311910/150‑1 (MQ=255)
                                  gtgtAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGAtt                                                                                                               <  2:4450765/150‑1 (MQ=255)
                                   tgtAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTa                                                                                                              <  2:2235321/150‑1 (MQ=255)
                                     taataaGCCGTAGCGTTGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAAc                                                                                                            <  2:4976950/150‑1 (MQ=255)
                                     taataaGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAAc                                                                                                            <  2:5148207/150‑1 (MQ=255)
                                     taataaGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAAc                                                                                                            <  2:3696633/150‑1 (MQ=255)
                                      aataaGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACg                                                                                                           <  2:1829199/150‑1 (MQ=255)
                                      aataaGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACg                                                                                                           <  2:5263834/150‑1 (MQ=255)
                                      aataaGCCGTAGCGTGGACAATACCGCCGATAAAACAGGCAGTTGCGAAGATTTCTGAAAGTAAAATCATGGGGATTTCGCGGGACAGAACATCACGAATGAACCCGCCTCCAACGCCAGAAATGACGCCCATACAAACCGCGATTAAct                                                                                                           >  1:4692360/1‑149 (MQ=255)
                                       ataaGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACgg                                                                                                          >  1:989889/1‑150 (MQ=255)
                                         aaGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGAc                                                                                                        <  1:3832647/150‑1 (MQ=255)
                                          aGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGAcc                                                                                                       <  1:3669029/150‑1 (MQ=255)
                                          aGCCGTAGAGTGGACAATACCGACGATAATACAGGCAGTTGCTTAGATTTATGTACGTAAAATCATGGGGATTTCGCTGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCATTAATGACGCCCATACAAACCGCGATTAACGGAcc                                                                                                       <  2:533016/150‑1 (MQ=255)
                                           gCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCg                                                                                                      <  2:4883889/150‑1 (MQ=255)
                                            ccGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACTCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCgg                                                                                                     >  2:3464131/1‑150 (MQ=255)
                                              gTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCt                                                                                                   >  1:843405/1‑150 (MQ=255)
                                              gTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCt                                                                                                   >  2:1382354/1‑150 (MQ=255)
                                              gTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCt                                                                                                   <  1:4364773/150‑1 (MQ=255)
                                               tAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCtt                                                                                                  >  1:2486566/1‑150 (MQ=255)
                                                aGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTc                                                                                                 >  1:1081553/1‑150 (MQ=255)
                                                  cGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCg                                                                                               >  1:4187765/1‑150 (MQ=255)
                                                    tGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCa                                                                                             >  2:2175474/1‑150 (MQ=255)
                                                     ggACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCAt                                                                                            <  1:3191148/150‑1 (MQ=255)
                                                        cAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTaa                                                                                         >  1:4509211/1‑150 (MQ=255)
                                                         aaTACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCGCCAACGCCAGTCATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTaaa                                                                                        >  2:2393377/1‑150 (MQ=255)
                                                         aaTACCGCCGATAATACAGGCAGTTGCGAAGATTTCTGTACGAAAAATCATGGGGATTTCGAGGGCCAGAACAACACGAATGATACCGCCACCAACGCCAGTAATGACGCCCATACAAACCGCGATTAAAGGACCGGCTTCCGAATTaaa                                                                                        >  1:1008197/1‑150 (MQ=255)
                                                           tACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAgg                                                                                      <  2:2829533/150‑1 (MQ=255)
                                                              cgccgATAATACAGGCAGTTGCGTAGATTTCTGTACGCAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCtt                                                                                   <  1:4840736/150‑1 (MQ=255)
                                                                    taataCAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGAGTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCa                                                                             >  2:3143019/1‑150 (MQ=255)
                                                                      ataCAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTCACGGACCGGCTTCCGCATTAAAGGCTTTATTCACg                                                                           >  1:2541663/1‑150 (MQ=255)
                                                                       taCAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGc                                                                          >  1:906189/1‑150 (MQ=255)
                                                                         cAGGCAGTTGCGTAGATTTCTTTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCa                                                                        <  1:1831648/150‑1 (MQ=255)
                                                                          aGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCaa                                                                       >  2:956878/1‑150 (MQ=255)
                                                                            gCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATg                                                                     <  1:3554253/150‑1 (MQ=255)
                                                                            gCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCAAACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATg                                                                     <  2:1901119/150‑1 (MQ=255)
                                                                             cAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGc                                                                    >  2:3935907/1‑150 (MQ=255)
                                                                                  gCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACa                                                               <  2:4897228/150‑1 (MQ=255)
                                                                                   cGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACaa                                                              >  2:624185/1‑150 (MQ=255)
                                                                                   cGTAGATATCTGTACGTAAAATCATGGGGAGTTCGCGGGCCAGAACATCAAGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATAAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGTCaa                                                              >  1:2696916/1‑150 (MQ=255)
                                                                                    gTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATg                                                                     <  1:1792427/142‑1 (MQ=255)
                                                                                    gTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACaaa                                                             <  2:4787492/150‑1 (MQ=255)
                                                                                     tAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAac                                                            <  1:4797604/150‑1 (MQ=255)
                                                                                     tAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAac                                                            <  1:2237648/150‑1 (MQ=255)
                                                                                     tAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAac                                                            <  2:857468/150‑1 (MQ=255)
                                                                                     tAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAac                                                            <  1:5216194/150‑1 (MQ=255)
                                                                                      aGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAaca                                                           <  1:1517580/150‑1 (MQ=255)
                                                                                      aGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAaca                                                           <  1:4823640/150‑1 (MQ=255)
                                                                                      aGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCACAAACGACAGTAATGAAGACCATACAAACCGCGATTAACGGAACGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCAGACAAaca                                                           >  1:1217124/1‑150 (MQ=255)
                                                                                        aTTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACAcc                                                         <  1:2148075/150‑1 (MQ=255)
                                                                                         tttCTGTACGAAAATTCATGGGGATTTCGCGGGCCAGAACATCTCGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAACCCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGTCTAACACCg                                                        <  2:4314533/150‑1 (MQ=255)
                                                                                            cTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCa                                                     >  1:163596/1‑150 (MQ=255)
                                                                                             tGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTACCGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAg                                                    >  2:2633744/1‑150 (MQ=255)
                                                                                             tGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAg                                                    >  2:1743283/1‑150 (MQ=255)
                                                                                             tGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAg                                                    <  1:3391186/150‑1 (MQ=255)
                                                                                                   tAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAAc                                              >  2:1291079/1‑150 (MQ=255)
                                                                                                    aaaaTCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACg                                             <  2:5053401/150‑1 (MQ=255)
                                                                                                    aaaaTCATGGGGATTTCGCGGGCAAGAACATAACGAATGATCCAGCCTCCAACTCCAGTAATGACGCACATACACACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACg                                             <  2:4692360/150‑1 (MQ=255)
                                                                                                     aaaTCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACgg                                            >  2:4019177/1‑150 (MQ=255)
                                                                                                      aaTCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGc                                           >  2:5220690/1‑150 (MQ=255)
                                                                                                      aaTCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGc                                           <  1:964680/150‑1 (MQ=255)
                                                                                                      aaTCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGc                                           >  2:5302509/1‑150 (MQ=255)
                                                                                                       aTCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTACAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCg                                          >  2:138965/1‑150 (MQ=255)
                                                                                                          aTGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTcc                                       >  1:2866307/1‑150 (MQ=255)
                                                                                                               gATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCAc                                  >  2:2599214/1‑150 (MQ=255)
                                                                                                                 tttCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCg                                <  2:3226722/150‑1 (MQ=255)
                                                                                                                      cgGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCACATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAAc                           <  2:4443059/150‑1 (MQ=255)
                                                                                                                         gCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATc                        >  1:681267/1‑150 (MQ=255)
                                                                                                                          ccAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATcc                       >  2:1986252/1‑150 (MQ=255)
                                                                                                                             gAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCAtt                    >  2:167405/1‑150 (MQ=255)
                                                                                                                              aaCATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACTGCCAGACCAACGGCGTCCAGCACCGGCAACATCCAttt                   >  1:2731211/1‑150 (MQ=255)
                                                                                                                                cATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTg                 <  2:3702148/150‑1 (MQ=255)
                                                                                                                                 aTCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTgg                >  1:4633731/1‑150 (MQ=255)
                                                                                                                                 aTCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTgg                >  1:537130/1‑150 (MQ=255)
                                                                                                                                    aCGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTaa             <  2:4634362/150‑1 (MQ=255)
                                                                                                                                     cGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAg            <  1:5183003/150‑1 (MQ=255)
                                                                                                                                       aaTGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATATTGGTAAGCg          >  1:3833398/1‑150 (MQ=255)
                                                                                                                                         tGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTc        <  1:2920934/150‑1 (MQ=255)
                                                                                                                                           aTCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCtt      >  1:4063778/1‑150 (MQ=255)
                                                                                                                                               cgcctcCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTtggct  <  2:1481629/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCGCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCT  >  CP009273/173578‑173870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCGCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGC  >  CP009273/173578‑173873
                                                                                                                                                   |                                                                                                                                                    
CATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGACTGATCCCGCCTCC                                                                                                                                                    >  SRR19973062.730357/1‑150 (MQ=60)
CATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCC                                                                                                                                                    >  SRR19973062.1026024/1‑150 (MQ=60)
  TCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAA                                                                                                                                                  <  SRR19973062.1995253/150‑1 (MQ=60)
      ACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCC                                                                                                                                              <  SRR19973062.208478/150‑1 (MQ=60)
       CTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCA                                                                                                                                             >  SRR19973062.517041/1‑150 (MQ=60)
       CTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCA                                                                                                                                             <  SRR19973062.2022808/150‑1 (MQ=60)
       CTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCA                                                                                                                                             <  SRR19973062.2022678/150‑1 (MQ=60)
       CTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCA                                                                                                                                             <  SRR19973062.324747/150‑1 (MQ=60)
          GCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTA                                                                                                                                          <  SRR19973062.1306562/150‑1 (MQ=60)
            TGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAAT                                                                                                                                        <  SRR19973062.1968157/150‑1 (MQ=60)
             GTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATG                                                                                                                                       <  SRR19973062.2903824/150‑1 (MQ=60)
             GTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAAAG                                                                                                                                       >  SRR19973062.3669908/1‑150 (MQ=60)
              TTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCTCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGA                                                                                                                                      <  SRR19973062.3669323/150‑1 (MQ=60)
                   AGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCC                                                                                                                                 <  SRR19973062.4857163/150‑1 (MQ=60)
                    GTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACCGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCA                                                                                                                                <  SRR19973062.329776/150‑1 (MQ=60)
                     TGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCAT                                                                                                                               >  SRR19973062.2893459/1‑150 (MQ=60)
                       GTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATAC                                                                                                                             <  SRR19973062.2312340/150‑1 (MQ=60)
                       GTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATAC                                                                                                                             <  SRR19973062.2717203/150‑1 (MQ=60)
                        TACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACA                                                                                                                            >  SRR19973062.4612476/1‑150 (MQ=60)
                            GAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACC                                                                                                                        >  SRR19973062.1172488/1‑150 (MQ=60)
                            GAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACC                                                                                                                        <  SRR19973062.5016365/150‑1 (MQ=60)
                              AAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGC                                                                                                                      >  SRR19973062.2296322/1‑150 (MQ=60)
                              AAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCAGGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGC                                                                                                                      >  SRR19973062.2296307/1‑150 (MQ=60)
                               AATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCG                                                                                                                     >  SRR19973062.246638/1‑150 (MQ=60)
                               AATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCG                                                                                                                     >  SRR19973062.4801686/1‑150 (MQ=60)
                               AATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCG                                                                                                                     >  SRR19973062.4425908/1‑150 (MQ=60)
                               AATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCG                                                                                                                     >  SRR19973062.4425918/1‑150 (MQ=60)
                                ATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGA                                                                                                                    >  SRR19973062.3316514/1‑150 (MQ=60)
                                 TGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGAT                                                                                                                   >  SRR19973062.3107057/1‑150 (MQ=60)
                                  GTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATT                                                                                                                  >  SRR19973062.4143838/1‑150 (MQ=60)
                                  GTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATT                                                                                                                  >  SRR19973062.4625469/1‑150 (MQ=60)
                                     TAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGAGTAAC                                                                                                               >  SRR19973062.3926716/1‑150 (MQ=60)
                                      AATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACG                                                                                                              >  SRR19973062.4717732/1‑150 (MQ=60)
                                      AATAAGCCGTAGCGTGGACAATACCGCCGATAAAACAGGCAGTTGCGAAGATTTCTGAAAGTAAAATCATGGGGATTTCGCGGGACAGAACATCACGAATGAACCCGCCTCCAACGCCAGAAATGACGCCCATACAAACCGCGATTAACT                                                                                                              >  SRR19973062.4716879/1‑150 (MQ=60)
                                       ATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGG                                                                                                             >  SRR19973062.993925/1‑150 (MQ=60)
                                       ATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGG                                                                                                             >  SRR19973062.994489/1‑150 (MQ=60)
                                       ATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAAAGATCCCGCCTCCAACGCCAGTAATGACGCCCATACACACCGCGATTAACGG                                                                                                             >  SRR19973062.164134/1‑150 (MQ=60)
                                         AAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGAC                                                                                                           <  SRR19973062.2435323/150‑1 (MQ=60)
                                         AAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGAC                                                                                                           >  SRR19973062.2480272/1‑150 (MQ=60)
                                         AAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGAC                                                                                                           <  SRR19973062.3852254/150‑1 (MQ=60)
                                          AGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACC                                                                                                          <  SRR19973062.3687845/150‑1 (MQ=60)
                                              GTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCT                                                                                                      >  SRR19973062.847071/1‑150 (MQ=60)
                                              GTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCT                                                                                                      <  SRR19973062.4387176/150‑1 (MQ=60)
                                               TAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTT                                                                                                     >  SRR19973062.2499880/1‑150 (MQ=60)
                                                AGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTC                                                                                                    >  SRR19973062.1086652/1‑150 (MQ=60)
                                                AGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTC                                                                                                    <  SRR19973062.4836933/150‑1 (MQ=60)
                                                  CGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCG                                                                                                  >  SRR19973062.4209492/1‑150 (MQ=60)
                                                  CGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCG                                                                                                  >  SRR19973062.4077385/1‑150 (MQ=60)
                                                   GTGGACAATACCGCCGTTAATTCAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCATGAACATCACGAATGTTCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGC                                                                                                 <  SRR19973062.2525132/150‑1 (MQ=60)
                                                   GTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGC                                                                                                 >  SRR19973062.3707653/1‑150 (MQ=60)
                                                     GGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCAT                                                                                               <  SRR19973062.3207715/150‑1 (MQ=60)
                                                       ACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTA                                                                                             <  SRR19973062.1177410/150‑1 (MQ=60)
                                                        CAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAA                                                                                            >  SRR19973062.4532595/1‑150 (MQ=60)
                                                         AATACCGCCGATAATACAGGCAGTTGCGAAGATTTCTGTACGAAAAATCATGGGGATTTCGAGGGCCAGAACAACACGAATGATACCGCCACCAACGCCAGTAATGACGCCCATACAAACCGCGATTAAAGGACCGGCTTCCGAATTAAA                                                                                           >  SRR19973062.1012906/1‑150 (MQ=60)
                                                           TACCGCCGATAATCCAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGG                                                                                         <  SRR19973062.4346908/150‑1 (MQ=60)
                                                              CGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGCAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTT                                                                                      <  SRR19973062.4865744/150‑1 (MQ=60)
                                                                 CGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTAT                                                                                   >  SRR19973062.268643/1‑150 (MQ=60)
                                                                 CGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTAT                                                                                   <  SRR19973062.1406068/150‑1 (MQ=60)
                                                                     AATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTAGTCAC                                                                               <  SRR19973062.1252288/150‑1 (MQ=60)
                                                                      ATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTCACGGACCGGCTTCCGCATTAAAGGCTTTATTCACG                                                                              >  SRR19973062.2555211/1‑150 (MQ=60)
                                                                       TACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGC                                                                             >  SRR19973062.18948/1‑150 (MQ=60)
                                                                       TACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGC                                                                             >  SRR19973062.910114/1‑150 (MQ=60)
                                                                         CAGGCAGTTGCGTAGATTTCTTTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCA                                                                           <  SRR19973062.1840715/150‑1 (MQ=60)
                                                                         CAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCA                                                                           <  SRR19973062.1840667/150‑1 (MQ=60)
                                                                          AGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAA                                                                          >  SRR19973062.928753/1‑150 (MQ=60)
                                                                            tccgatctGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATG                                                                        <  SRR19973062.1801100/142‑1 (MQ=60)
                                                                            GCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATG                                                                        <  SRR19973062.3572191/150‑1 (MQ=60)
                                                                             CAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGC                                                                       >  SRR19973062.2529199/1‑150 (MQ=60)
                                                                                 TGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGAC                                                                   <  SRR19973062.4512890/150‑1 (MQ=60)
                                                                                   CGTAGATATCTGTACGTAAAATCATGGGGAGTTCGCGGGCCAGAACATCAAGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATAAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGTCAA                                                                 >  SRR19973062.2711048/1‑150 (MQ=60)
                                                                                     TAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAAC                                                               <  SRR19973062.4822490/150‑1 (MQ=60)
                                                                                     TAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAAC                                                               <  SRR19973062.5243707/150‑1 (MQ=60)
                                                                                     TAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAAC                                                               <  SRR19973062.2248829/150‑1 (MQ=60)
                                                                                      AGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACA                                                              >  SRR19973062.5305181/1‑150 (MQ=60)
                                                                                      AGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACA                                                              <  SRR19973062.1525057/150‑1 (MQ=60)
                                                                                      AGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACA                                                              <  SRR19973062.4848602/150‑1 (MQ=60)
                                                                                      AGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCACAAACGACAGTAATGAAGACCATACAAACCGCGATTAACGGAACGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCAGACAAACA                                                              >  SRR19973062.1222785/1‑150 (MQ=60)
                                                                                        ATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACC                                                            <  SRR19973062.812124/150‑1 (MQ=60)
                                                                                        ATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACC                                                            <  SRR19973062.1277603/150‑1 (MQ=60)
                                                                                        ATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACC                                                            <  SRR19973062.2158967/150‑1 (MQ=60)
                                                                                         TTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCG                                                           >  SRR19973062.522932/1‑150 (MQ=60)
                                                                                           TCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCC                                                         <  SRR19973062.4854710/150‑1 (MQ=60)
                                                                                           TCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCC                                                         <  SRR19973062.2120527/150‑1 (MQ=60)
                                                                                            CTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCA                                                        >  SRR19973062.164514/1‑150 (MQ=60)
                                                                                             TGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAG                                                       <  SRR19973062.3408594/150‑1 (MQ=60)
                                                                                               TACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGAC                                                     <  SRR19973062.1805282/150‑1 (MQ=60)
                                                                                               TACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGAC                                                     >  SRR19973062.854795/1‑150 (MQ=60)
                                                                                               TACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGAC                                                     >  SRR19973062.855277/1‑150 (MQ=60)
                                                                                                 CGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCA                                                   >  SRR19973062.4254992/1‑150 (MQ=60)
                                                                                                  GTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAACCCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAA                                                  <  SRR19973062.2357544/150‑1 (MQ=60)
                                                                                                   TAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAAC                                                 <  SRR19973062.583210/150‑1 (MQ=60)
                                                                                                   TAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAAC                                                 <  SRR19973062.583217/150‑1 (MQ=60)
                                                                                                      AATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGC                                              <  SRR19973062.969115/150‑1 (MQ=60)
                                                                                                      AATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGC                                              <  SRR19973062.969019/150‑1 (MQ=60)
                                                                                                      AATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGC                                              >  SRR19973062.4185037/1‑150 (MQ=60)
                                                                                                          ATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCC                                          >  SRR19973062.2881468/1‑150 (MQ=60)
                                                                                                          ATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCC                                          <  SRR19973062.4097848/150‑1 (MQ=60)
                                                                                                            GGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAG                                        >  SRR19973062.2806016/1‑150 (MQ=60)
                                                                                                            GGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAG                                        <  SRR19973062.3852758/150‑1 (MQ=60)
                                                                                                              GGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCA                                      >  SRR19973062.5013426/1‑150 (MQ=60)
                                                                                                                ATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACC                                    <  SRR19973062.1735062/150‑1 (MQ=60)
                                                                                                                 TTTCGCGGGCCAGAACATCACGAAAGATCCCGCATCCAACTCCAGTAATGACGCCCATACAAACCTCGAATAACCGACCGGCTTCCGCAATAAAGTCTAAATTCACGCCAATGCCCACAAACACCGCCAGACCAACGGCGTCCAGCACCG                                   >  SRR19973062.33255/1‑150 (MQ=60)
                                                                                                                  TTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGG                                  <  SRR19973062.3408719/150‑1 (MQ=60)
                                                                                                                      CGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAAC                              <  SRR19973062.44007/150‑1 (MQ=60)
                                                                                                                         GCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATC                           >  SRR19973062.684433/1‑150 (MQ=60)
                                                                                                                           CAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCA                         >  SRR19973062.1575928/1‑150 (MQ=60)
                                                                                                                            AGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCAT                        >  SRR19973062.5190719/1‑150 (MQ=60)
                                                                                                                             GAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATT                       >  SRR19973062.3006209/1‑150 (MQ=60)
                                                                                                                              AACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACTGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTT                      >  SRR19973062.2745593/1‑150 (MQ=60)
                                                                                                                              AACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACTGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTT                      >  SRR19973062.2744901/1‑150 (MQ=60)
                                                                                                                              AACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTT                      >  SRR19973062.4697472/1‑150 (MQ=60)
                                                                                                                               ACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTT                     <  SRR19973062.4142363/150‑1 (MQ=60)
                                                                                                                               ACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTT                     >  SRR19973062.3502788/1‑150 (MQ=60)
                                                                                                                               ACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTT                     >  SRR19973062.2478120/1‑150 (MQ=60)
                                                                                                                                CATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTG                    <  SRR19973062.2754404/150‑1 (MQ=60)
                                                                                                                                CATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCAGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTG                    >  SRR19973062.2222685/1‑150 (MQ=60)
                                                                                                                                 ATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGG                   >  SRR19973062.539663/1‑150 (MQ=60)
                                                                                                                                 ATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGG                   >  SRR19973062.4657990/1‑150 (MQ=60)
                                                                                                                                 ATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGG                   >  SRR19973062.4657977/1‑150 (MQ=60)
                                                                                                                                    ACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCTACGGCGTCGAGCTCCGGCAACATCCATTTTGGTAA                <  SRR19973062.3851142/150‑1 (MQ=60)
                                                                                                                                     CGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACACACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAG               <  SRR19973062.152615/150‑1 (MQ=60)
                                                                                                                                     CGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAG               <  SRR19973062.5210365/150‑1 (MQ=60)
                                                                                                                                     CGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAG               <  SRR19973062.5210094/150‑1 (MQ=60)
                                                                                                                                       AATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATATTGGTAAGCG             >  SRR19973062.3853010/1‑150 (MQ=60)
                                                                                                                                         TGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTC           <  SRR19973062.1878023/150‑1 (MQ=60)
                                                                                                                                         TGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTC           <  SRR19973062.2936323/150‑1 (MQ=60)
                                                                                                                                           ATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTT         <  SRR19973062.5239006/150‑1 (MQ=60)
                                                                                                                                           ATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTT         >  SRR19973062.4084614/1‑150 (MQ=60)
                                                                                                                                           ATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTT         >  SRR19973062.4163886/1‑150 (MQ=60)
                                                                                                                                                  CTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGC  >  SRR19973062.2805514/1‑150 (MQ=60)
                                                                                                                                                  CTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGC  >  SRR19973062.2805506/1‑150 (MQ=60)
                                                                                                                                                  CTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGC  >  SRR19973062.1276455/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                    
CATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCGCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGC  >  CP009273/173578‑173873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑