Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 517,551 G→T 24.0% P560P (CCG→CCT)  ybbP → putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273517,5510GT24.0% 688.4 / 157.6 289P560P (CCG→CCT) ybbPputative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (128/91);  new base T (42/28);  total (170/119)
Fisher's exact test for biased strand distribution p-value = 8.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 294 total aligned reads displayed.

GAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGT  >  CP009273/517402‑517700
                                                                                                                                                      |                                                                                                                                                     
gAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACACAAACGTACCAGGC‑AAAATACGCGGCCCGATCATAAACCg                                                                                                                                                       >  1:4923878/1‑150 (MQ=255)
 aaCATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGa                                                                                                                                                      >  2:4622586/1‑150 (MQ=255)
   cATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGAtt                                                                                                                                                    <  1:4166716/150‑1 (MQ=255)
     tCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCTATTGt                                                                                                                                                  <  1:3084279/150‑1 (MQ=255)
         aTAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACTCGGCCCGATCATAATCCGATTGTCGcc                                                                                                                                              >  2:1564718/1‑150 (MQ=255)
            gTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGt                                                                                                                                           <  2:3498034/150‑1 (MQ=255)
              cccGGAATCGTTTTATCCGGGGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAATGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTAACTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTaa                                                                                                                                         >  1:2710517/1‑150 (MQ=255)
               ccGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAAc                                                                                                                                        <  2:5238468/150‑1 (MQ=255)
                cGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACt                                                                                                                                       <  1:127944/150‑1 (MQ=255)
                cGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAAACCTATTGTCGCCGGTAACt                                                                                                                                       >  2:4476107/1‑150 (MQ=255)
                 ggAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAAACCTATTGTCGCCGGTAACTg                                                                                                                                      >  1:2424853/1‑150 (MQ=255)
                  gAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCTATTGTCGCCGGTAACTgg                                                                                                                                     <  2:4163036/150‑1 (MQ=255)
                  gAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTgg                                                                                                                                     >  2:3623151/1‑150 (MQ=255)
                    aTCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGgcc                                                                                                                                   >  2:308173/1‑150 (MQ=255)
                        ttttATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCa                                                                                                                               >  1:1604673/1‑150 (MQ=255)
                        ttttATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCa                                                                                                                               <  2:4058730/150‑1 (MQ=255)
                            aTCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCTATTGTCGCCGGTAACTGGCCGCCAAAAg                                                                                                                           <  1:2594134/150‑1 (MQ=255)
                            aTCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAg                                                                                                                           >  1:4106716/1‑150 (MQ=255)
                              ccGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCTATTGTCGCCGGTAACTGGCCGCCAAAAGcc                                                                                                                         <  1:1472654/150‑1 (MQ=255)
                                 gtggtgCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCTATTGTCGCCGGTAACTGGCCGCC‑AAAGCCGATg                                                                                                                     <  2:2178565/150‑1 (MQ=255)
                                 gtggtgCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGAt                                                                                                                      <  2:1531119/150‑1 (MQ=255)
                                   ggtgCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCTATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGa                                                                                                                    >  1:600002/1‑150 (MQ=255)
                                       cGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGACgtg                                                                                                                >  1:3728393/1‑150 (MQ=255)
                                       cGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAgtg                                                                                                                <  2:3860997/150‑1 (MQ=255)
                                          gcgcGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCg                                                                                                             <  2:2308466/150‑1 (MQ=255)
                                            gcgGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCTATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGAt                                                                                                           >  1:2910671/1‑150 (MQ=255)
                                             cgGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATg                                                                                                          >  1:1390083/1‑150 (MQ=255)
                                             cgGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATg                                                                                                          >  1:3418006/1‑150 (MQ=255)
                                                cTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGaa                                                                                                       >  1:839983/1‑150 (MQ=255)
                                                cTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGaa                                                                                                       >  2:628566/1‑150 (MQ=255)
                                                 tGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAag                                                                                                      >  2:4356169/1‑150 (MQ=255)
                                                   aCGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCTATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAagag                                                                                                    <  2:2910671/150‑1 (MQ=255)
                                                         aTTAACGATACGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATCCGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTg                                                                                              <  1:1007002/150‑1 (MQ=255)
                                                         aTTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCTATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTg                                                                                              <  1:2515617/150‑1 (MQ=255)
                                                          ttAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTgg                                                                                             <  1:3749887/150‑1 (MQ=255)
                                                          ttAAAGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTg                                                                                              <  2:678520/150‑1 (MQ=255)
                                                           tAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGc                                                                                            >  1:3241399/1‑150 (MQ=255)
                                                           tAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGc                                                                                            >  1:5245361/1‑150 (MQ=255)
                                                            aaCGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCg                                                                                           >  1:1786127/1‑150 (MQ=255)
                                                               gATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCTATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGaaa                                                                                        >  2:2231381/1‑150 (MQ=255)
                                                                aTAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCTATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAAc                                                                                       >  1:3383036/1‑150 (MQ=255)
                                                                aTAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAAc                                                                                       <  2:4825878/150‑1 (MQ=255)
                                                                      cGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTaa                                                                                 <  2:3415278/150‑1 (MQ=255)
                                                                      cGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTaa                                                                                 >  1:3178694/1‑150 (MQ=255)
                                                                         cAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACg                                                                              <  2:1112277/150‑1 (MQ=255)
                                                                         cAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACg                                                                              <  2:1017237/150‑1 (MQ=255)
                                                                          aGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGt                                                                             >  2:4286006/1‑150 (MQ=255)
                                                                           gAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGtt                                                                            >  2:2644114/1‑150 (MQ=255)
                                                                              ggTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGcc                                                                         <  1:1705995/150‑1 (MQ=255)
                                                                              ggTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCCCCGGAACCTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGcc                                                                         >  2:995852/1‑150 (MQ=255)
                                                                               gTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGccc                                                                        >  2:3961370/1‑150 (MQ=255)
                                                                               gTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGccc                                                                        >  1:722758/1‑150 (MQ=255)
                                                                                 aaTGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTc                                                                      >  1:1985425/1‑150 (MQ=255)
                                                                                 aaTGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTc                                                                      <  2:210559/150‑1 (MQ=255)
                                                                                 aaTGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGAGGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTc                                                                      >  2:4018261/1‑150 (MQ=255)
                                                                                     aaGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCTATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTg                                                                  >  1:2818607/1‑150 (MQ=255)
                                                                                      aGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCTATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGa                                                                 >  2:3050931/1‑150 (MQ=255)
                                                                                       gATGAGGCGCTTAACCGCGAACTAAATCTTACCTGTC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGAt                                                                <  1:4484143/150‑1 (MQ=255)
                                                                                         tGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATAc                                                              <  1:361887/150‑1 (MQ=255)
                                                                                          gAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCTATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATAcc                                                             >  1:1128732/1‑150 (MQ=255)
                                                                                          gAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATAcc                                                             <  2:1418432/150‑1 (MQ=255)
                                                                                               gcTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGAc                                                        >  1:2976463/1‑150 (MQ=255)
                                                                                               gcTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGAc                                                        >  1:5304086/1‑150 (MQ=255)
                                                                                                 ttAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACtt                                                      >  2:2364883/1‑150 (MQ=255)
                                                                                                  tAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACttt                                                     <  2:2773765/150‑1 (MQ=255)
                                                                                                       gcgATCTCATTCTTTCCTGGC‑TAAATCCGCGGCCCGATCATAATCCGATTGTCGGCGGTAACTGGCCGCCAACAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGACACGCTTAATCGTTGCCCTCGGTGATACCGTGACTTTTATgg                                                <  2:5047827/150‑1 (MQ=255)
                                                                                                       gcgAACTCAATCTTACCTGGC‑AAACTACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATgg                                                <  2:2739715/150‑1 (MQ=255)
                                                                                                        cgAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATggg                                               >  1:1054417/1‑150 (MQ=255)
                                                                                                         gAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGc                                              >  1:1930758/1‑150 (MQ=255)
                                                                                                          aaCTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTGTTATGGGCg                                             >  1:4012641/1‑150 (MQ=255)
                                                                                                            cTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCTATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGAt                                           >  1:1980226/1‑150 (MQ=255)
                                                                                                              cAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATAc                                         >  1:1029405/1‑150 (MQ=255)
                                                                                                              cAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGACAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATAc                                         <  2:1167718/150‑1 (MQ=255)
                                                                                                               aaTCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATAcc                                        <  1:465590/150‑1 (MQ=255)
                                                                                                                   ttACCTGGC‑AAAATACGCGGCCCGATCATAATCCTATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAgg                                    <  1:1562366/150‑1 (MQ=255)
                                                                                                                   ttACCTGGC‑AAAATACGCGGCCCGATCATAATCCTATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAgg                                    >  1:1211024/1‑150 (MQ=255)
                                                                                                                   ttACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAgg                                    <  1:804868/150‑1 (MQ=255)
                                                                                                                    tACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGa                                   >  2:2460419/1‑150 (MQ=255)
                                                                                                                     aCCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAg                                  >  2:3690303/1‑150 (MQ=255)
                                                                                                                        tGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTc                               >  1:3666471/1‑150 (MQ=255)
                                                                                                                         ggC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTcc                              <  2:2710517/150‑1 (MQ=255)
                                                                                                                           c‑AAAATACGCGGCCCGATCATAATCCTATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCcgc                            >  1:672542/1‑150 (MQ=255)
                                                                                                                           c‑AAAATACGCGGCCCGATCATAATCCTATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCcgc                            >  1:4326629/1‑150 (MQ=255)
                                                                                                                                 tACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTaa                       >  2:4970466/1‑150 (MQ=255)
                                                                                                                                 tACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTaa                       <  2:2522256/150‑1 (MQ=255)
                                                                                                                                    gcgGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGt                    <  1:2195724/150‑1 (MQ=255)
                                                                                                                                     cgGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTg                   >  1:3770846/1‑150 (MQ=255)
                                                                                                                                      ggCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGa                  >  1:3089984/1‑150 (MQ=255)
                                                                                                                                      ggCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGa                  >  2:3189897/1‑150 (MQ=255)
                                                                                                                                       gCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGAc                 >  2:1227132/1‑150 (MQ=255)
                                                                                                                                         ccGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCa               >  2:1881396/1‑150 (MQ=255)
                                                                                                                                           gATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGc             >  1:1729333/1‑150 (MQ=255)
                                                                                                                                           gATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGc             >  2:1333929/1‑150 (MQ=255)
                                                                                                                                            aTCATAATCCTATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGcc            >  1:2222757/1‑150 (MQ=255)
                                                                                                                                              cATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTg          >  1:3781745/1‑150 (MQ=255)
                                                                                                                                               aTAATCCTATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTgc         >  2:3770620/1‑150 (MQ=255)
                                                                                                                                               aTAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTgc         >  1:4745934/1‑150 (MQ=255)
                                                                                                                                                tAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTgcg        >  2:2042563/1‑150 (MQ=255)
                                                                                                                                                 aaTCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTgcgc       >  1:497372/1‑150 (MQ=255)
                                                                                                                                                      gATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGt  >  2:4148689/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC‑AAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGT  >  CP009273/517402‑517700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A