Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 715,924 C→T 33.8% intergenic (‑8/‑115) speF ← / → ybfK ornithine decarboxylase isozyme, inducible/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273715,9240CT33.8% 435.2 / 305.1 317intergenic (‑8/‑115)speF/ybfKornithine decarboxylase isozyme, inducible/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (101/108);  new base T (60/47);  total (162/155)
Fisher's exact test for biased strand distribution p-value = 2.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 337 total aligned reads displayed.

GTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGAATGCAGAAACCCTTACCAGTCAGGTGGCTGATAAT  >  CP009273/715775‑716073
                                                                                                                                                     |                                                                                                                                                     
gTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACAGTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCAtc                                                                                                                                                       <  1:4799240/150‑1 (MQ=255)
 ttttCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCAtct                                                                                                                                                      <  1:2684632/150‑1 (MQ=255)
 ttttCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCAtct                                                                                                                                                      <  2:5136735/150‑1 (MQ=255)
   ttCCTCATGTAACATCGTTGAGCGATAAAACTATTGAAGCCACATCGATATTACGACTTTAATTAATTTAGATAAATTCTCGCTGCGTGGTAAAACAGTACTGGAAAGAATCTATAACCGCAATTTTTAATTTTGACATTTTTCATttct                                                                                                                                                    <  1:2077670/150‑1 (MQ=255)
   ttCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATctct                                                                                                                                                    <  2:3182438/150‑1 (MQ=255)
      cGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTa                                                                                                                                                 <  1:1525707/150‑1 (MQ=255)
      cGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTa                                                                                                                                                 <  2:3073814/150‑1 (MQ=255)
      cGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTa                                                                                                                                                 <  2:4614732/150‑1 (MQ=255)
       tcATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTAt                                                                                                                                                <  2:4199074/149‑1 (MQ=255)
       gCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTAt                                                                                                                                                <  2:1628920/150‑1 (MQ=255)
         aTGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATTTCTTTAttt                                                                                                                                              >  1:140491/1‑150 (MQ=255)
              aCATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATTTCTTTATTTTAGGt                                                                                                                                         <  1:3261586/150‑1 (MQ=255)
                aTCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATTTCTTTATTTTAGGTaa                                                                                                                                       >  1:3639973/1‑150 (MQ=255)
                aTCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATTTCTTTATTTTAGGTaa                                                                                                                                       >  2:2900502/1‑150 (MQ=255)
                 tCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAAt                                                                                                                                      <  2:4502861/150‑1 (MQ=255)
                 tCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAAt                                                                                                                                      <  1:2804040/150‑1 (MQ=255)
                   gTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTCATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTCGGTAATAg                                                                                                                                    <  2:4782119/150‑1 (MQ=255)
                     tGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCACTTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCa                                                                                                                                  >  2:4841794/1‑150 (MQ=255)
                     tGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATTTCTTTATTTTAGGTAATAGCa                                                                                                                                  >  2:4887845/1‑150 (MQ=255)
                       aGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATTTCTTTATTTTAGGTAATAGCAgg                                                                                                                                >  2:1845172/1‑150 (MQ=255)
                          gctacaccTAGTGCCGCCACCTCGATATTCCGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTACCCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTGAGGTAATTGCAGGTcc                                                                                                                             <  2:332130/143‑1 (MQ=255)
                          gATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTcc                                                                                                                             >  2:2865321/1‑150 (MQ=255)
                           aTAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATTTCTTTATTTTAGGTAATAGCAGGTCCt                                                                                                                            >  2:663572/1‑150 (MQ=255)
                            tAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATTTCTTTATTTTAGGTAATAGCAGGTCCTc                                                                                                                           >  1:2214184/1‑150 (MQ=255)
                             aaaaCTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCa                                                                                                                          >  2:901205/1‑150 (MQ=255)
                             aaaaCTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCa                                                                                                                          <  1:2576783/150‑1 (MQ=255)
                               aaCTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAAt                                                                                                                        <  1:3109152/150‑1 (MQ=255)
                               aaCTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAAt                                                                                                                        <  1:1277071/150‑1 (MQ=255)
                                aCTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACTTTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTTACCGCATTTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAAtt                                                                                                                       <  2:1518905/150‑1 (MQ=255)
                                 cTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATTTCTTTATTTTAGGTAATAGCAGGTCCTCAAttt                                                                                                                      <  1:3950357/150‑1 (MQ=255)
                                 cTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAAttt                                                                                                                      >  2:2672773/1‑150 (MQ=255)
                                  tATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTtc                                                                                                                     <  2:175043/150‑1 (MQ=255)
                                   aTTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATTTCTTTATTTTAGGTAATAGCAGGTCCTCAATTtct                                                                                                                    >  1:4646362/1‑150 (MQ=255)
                                   aTTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTtct                                                                                                                    <  1:5301859/150‑1 (MQ=255)
                                     tGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCg                                                                                                                  <  1:2264212/150‑1 (MQ=255)
                                         gccACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATg                                                                                                              <  2:158555/150‑1 (MQ=255)
                                               tCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATTTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAat                                                                                                        <  1:88998/150‑1 (MQ=255)
                                               tCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAat                                                                                                        <  2:2562541/150‑1 (MQ=255)
                                                 gATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAatcg                                                                                                      >  1:5189748/1‑150 (MQ=255)
                                                  atatTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAatcga                                                                                                     >  1:5007042/1‑150 (MQ=255)
                                                          aCTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATTTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCg                                                                                             >  2:406191/1‑150 (MQ=255)
                                                           cTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCgg                                                                                            >  1:1750884/1‑150 (MQ=255)
                                                               cATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATTTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGaaaa                                                                                        >  1:562715/1‑150 (MQ=255)
                                                                   aaTGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATTTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGc                                                                                    >  1:1418696/1‑150 (MQ=255)
                                                                       tAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAg                                                                                <  2:1787147/150‑1 (MQ=255)
                                                                        aGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGt                                                                               <  1:154223/150‑1 (MQ=255)
                                                                         gATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGtt                                                                              <  2:2151000/150‑1 (MQ=255)
                                                                          aTACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTa                                                                             >  1:1151843/1‑150 (MQ=255)
                                                                            aCATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTAcc                                                                           <  2:3673875/150‑1 (MQ=255)
                                                                              aTTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTAcccc                                                                         >  1:3791454/1‑150 (MQ=255)
                                                                                 ctcGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCt                                                                      >  1:3890487/1‑150 (MQ=255)
                                                                                   cGCTGCGTGGTAAAACTGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCACTATATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGGGCCGGAAAAAAGCAAAGTTACCCCGCTGa                                                                    <  1:2300549/150‑1 (MQ=255)
                                                                                   cGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATTTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGa                                                                    >  1:4957738/1‑150 (MQ=255)
                                                                                    gCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGat                                                                   >  1:4064441/1‑150 (MQ=255)
                                                                                     cTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATTTCTTTATGTTAGGTAATAGCAGGTCCTCCATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGata                                                                  >  2:2697851/1‑150 (MQ=255)
                                                                                        cGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATGTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGataaat                                                               >  2:4240738/1‑150 (MQ=255)
                                                                                         gTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATTTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGataaata                                                              >  2:847892/1‑150 (MQ=255)
                                                                                           ggTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATTTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAAGGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGAtaaataaa                                                            >  1:1848793/1‑150 (MQ=255)
                                                                                            gTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATaaataaat                                                           <  2:4813485/150‑1 (MQ=255)
                                                                                            gTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATaaataaat                                                           <  2:399788/150‑1 (MQ=255)
                                                                                              aaaaCAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCa                                                         >  2:1044208/1‑150 (MQ=255)
                                                                                              aaaaCAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCa                                                         <  1:3417359/150‑1 (MQ=255)
                                                                                               aaaCAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAg                                                        >  1:2389589/1‑150 (MQ=255)
                                                                                                  cAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATTTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTa                                                     <  2:2069729/150‑1 (MQ=255)
                                                                                                       cGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTg                                                <  1:1007048/150‑1 (MQ=255)
                                                                                                       cGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTg                                                <  1:707359/150‑1 (MQ=255)
                                                                                                          gCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCtt                                             >  1:242846/1‑150 (MQ=255)
                                                                                                           cAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCttt                                            >  2:3766674/1‑150 (MQ=255)
                                                                                                             aGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCGTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCttttt                                          >  2:4702082/1‑150 (MQ=255)
                                                                                                              gAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATTTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCtttttt                                         <  1:2693751/150‑1 (MQ=255)
                                                                                                              gAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATTTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCtttttt                                         <  1:4887845/150‑1 (MQ=255)
                                                                                                               aaTCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTc                                        >  1:5043460/1‑150 (MQ=255)
                                                                                                                 tCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAg                                      >  2:1112116/1‑150 (MQ=255)
                                                                                                                  cACTAACCGCAATTTTTAATTTTGACATTTTTCATTTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGa                                     >  2:1287451/1‑150 (MQ=255)
                                                                                                                   aCTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGaa                                    >  1:2888178/1‑150 (MQ=255)
                                                                                                                     tAACCGCAATTTTTAATTTTGACATTTTTCATTTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGAATg                                  <  2:2719441/150‑1 (MQ=255)
                                                                                                                       aCCGCAATTTTTAATTTTGACATTTTTCATTTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGAATGCa                                <  1:4277364/150‑1 (MQ=255)
                                                                                                                         cGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTATTTTCAGAATGCAGa                              <  1:4841408/150‑1 (MQ=255)
                                                                                                                            aaTTTTTAATTTTGACATTTTTCATTTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGAATGCAGAAAc                           >  2:2444502/1‑150 (MQ=255)
                                                                                                                            aaTTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGTAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGAATGCAGAAAc                           >  2:494162/1‑150 (MQ=255)
                                                                                                                             aTTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGAATGCAGAAAcc                          >  1:4345781/1‑150 (MQ=255)
                                                                                                                                 ttAATTTTGACATTTTTCATTTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGAATGCAGAAACCCTTa                      <  1:2155925/150‑1 (MQ=255)
                                                                                                                                 ttAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGAATGCAGAAACCCTTa                      >  1:509968/1‑150 (MQ=255)
                                                                                                                                 ttAATTTTGACATTTTTCATCTCATTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGAATGCAGAAACCCTTa                      <  1:3735399/150‑1 (MQ=255)
                                                                                                                                   aaTTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGAATGCAGAAACCCTTAcc                    <  2:2313728/150‑1 (MQ=255)
                                                                                                                                    aTTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGAATGCAGAAACCCTTACCa                   >  1:2069402/1‑150 (MQ=255)
                                                                                                                                    aTTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGAATGCAGAAACCCTTACCa                   <  1:1941668/150‑1 (MQ=255)
                                                                                                                                     ttttGACATTTTTCATTTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGGAGATTGCTTTTTTCAGAATGCAGAACCCCTTACCAg                  >  1:3500325/1‑150 (MQ=255)
                                                                                                                                      tttGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGAATGCAGAAACCCTTACCAGt                 <  1:4589447/150‑1 (MQ=255)
                                                                                                                                        tGACATTTTTCATTTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGAATGCAGAAACCCTTACCAGTCa               <  2:577880/150‑1 (MQ=255)
                                                                                                                                         gACATTTTTCATTTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGAATGCAGAAACCCTTACCAGTCAg              <  1:360838/150‑1 (MQ=255)
                                                                                                                                            aTTTTTCATTTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGAATGCAGAAACCCTTACCAGTCAGGTg           >  1:2511169/1‑150 (MQ=255)
                                                                                                                                              ttttCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGAATGCAGAAACACTTACCAGTCAGGTGGc         >  2:4416334/1‑150 (MQ=255)
                                                                                                                                               tttCATTTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGAATGCAGAAACCCTTACCAGTCAGGTGGCt        <  2:4114837/150‑1 (MQ=255)
                                                                                                                                                ttCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGAATGCAGAAACCCTTACCAGTCAGGTGGCTg       <  1:3009710/150‑1 (MQ=255)
                                                                                                                                                 tCATTTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGAATGCAGAAACCCTTACCAGTCAGGTGGCTGa      <  2:3711377/150‑1 (MQ=255)
                                                                                                                                                   aTCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGAATGCAGAAACCCTTACCAGTCAGGTGGCTGata    >  1:1109437/1‑150 (MQ=255)
                                                                                                                                                    tctcTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTCGATAGCTTTTTTCAGAATGCAGAAACCCTTACCAGTCAGGTGGCTGataa   >  2:5327676/1‑150 (MQ=255)
                                                                                                                                                    tctcTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGAATGCAGAAACCCTTACCAGTCAGGTGGCTGataa   >  1:5111315/1‑150 (MQ=255)
                                                                                                                                                     ctctTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGAATGCAGAAACCCTTACCAGTCAGGTGGCTGataat  >  2:5040554/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTATTTTAGGTAATAGCAGGTCCTCAATTTCTCGAATGAGAAATCGATCGTGCCGGAAAAAAGCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGAATGCAGAAACCCTTACCAGTCAGGTGGCTGATAAT  >  CP009273/715775‑716073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A