Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,055,132 G→T 100% E141* (GAA→TAA)  torA → trimethylamine N‑oxide (TMAO) reductase I, catalytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,055,1320GT99.0% 700.5 / ‑1.9 227E141* (GAA→TAA) torAtrimethylamine N‑oxide (TMAO) reductase I, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base T (98/125);  total (100/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 230 total aligned reads displayed.

GGATCACGTACACAACGCGGCGCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAAGAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATAGCGTTGGTACGGGCGGAG  >  CP009273/1054984‑1055276
                                                                                                                                                    |                                                                                                                                                
  atCACGTACACAACGCGGCGCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAAc                                                                                                                                               <  1:3331337/150‑1 (MQ=255)
   tCACGTACACAACGCGGCGCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGACAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACt                                                                                                                                              <  1:2503122/150‑1 (MQ=255)
     aCGTACACAACGCGGCGCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTgg                                                                                                                                            <  2:4810915/150‑1 (MQ=255)
     aCGTACACAACGCGGCGCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTgg                                                                                                                                            >  1:886490/1‑150 (MQ=255)
       gTACACAACGCGGCGCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGaa                                                                                                                                          >  1:4138975/1‑150 (MQ=255)
         acacAACGCGGCGCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAAcg                                                                                                                                        >  1:2578126/1‑150 (MQ=255)
          cacaACGCGGCGCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAAcgc                                                                                                                                       >  1:2107531/1‑150 (MQ=255)
                  ggcgCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGaa                                                                                                                               >  1:1441097/1‑150 (MQ=255)
                  ggcgCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGaa                                                                                                                               >  2:4097392/1‑150 (MQ=255)
                      cgTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACt                                                                                                                           >  2:2271751/1‑150 (MQ=255)
                      cgTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACt                                                                                                                           <  2:5193391/150‑1 (MQ=255)
                      cgTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACt                                                                                                                           <  2:2304049/150‑1 (MQ=255)
                      cgTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACt                                                                                                                           <  2:445600/150‑1 (MQ=255)
                      cgTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACt                                                                                                                           <  2:2837397/150‑1 (MQ=255)
                        tATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCa                                                                                                                         <  1:4257688/150‑1 (MQ=255)
                         aTTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCAc                                                                                                                        >  2:323379/1‑150 (MQ=255)
                         aTTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCAc                                                                                                                        >  2:3147462/1‑150 (MQ=255)
                            cGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACggg                                                                                                                     <  2:111150/150‑1 (MQ=255)
                            cGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACggg                                                                                                                     <  1:5151264/150‑1 (MQ=255)
                             gTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGACAACTCACGGGc                                                                                                                    >  2:2223504/1‑150 (MQ=255)
                             gTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGc                                                                                                                    <  1:3854804/150‑1 (MQ=255)
                                aTCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGa                                                                                                                 >  1:3259918/1‑150 (MQ=255)
                                 tCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAg                                                                                                                <  1:4677501/150‑1 (MQ=255)
                                 tCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAg                                                                                                                >  1:2315189/1‑150 (MQ=255)
                                 tCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAg                                                                                                                <  2:134533/150‑1 (MQ=255)
                                  ccGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGt                                                                                                               <  2:795410/150‑1 (MQ=255)
                                    gATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGc                                                                                                             <  2:3414343/150‑1 (MQ=255)
                                    gATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGc                                                                                                             <  2:4684953/150‑1 (MQ=255)
                                       ggTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCtt                                                                                                          <  1:715377/150‑1 (MQ=255)
                                        gTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTg                                                                                                         <  2:3094762/150‑1 (MQ=255)
                                        gTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTg                                                                                                         <  1:910997/150‑1 (MQ=255)
                                        gTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAAGAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTg                                                                                                         <  2:677658/150‑1 (MQ=255)
                                          aCGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCt                                                                                                       <  2:4480454/150‑1 (MQ=255)
                                             cgTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGAc                                                                                                    >  2:1571343/1‑150 (MQ=255)
                                              gTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGAcc                                                                                                   >  2:3706389/1‑150 (MQ=255)
                                               tGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCg                                                                                                  >  2:142171/1‑150 (MQ=255)
                                                 gACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGcc                                                                                                <  2:4815974/150‑1 (MQ=255)
                                                  aCTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCa                                                                                               <  2:2967752/150‑1 (MQ=255)
                                                    tGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGt                                                                                             >  2:4551472/1‑150 (MQ=255)
                                                    tGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGt                                                                                             >  2:4639308/1‑150 (MQ=255)
                                                     ggCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTg                                                                                            <  1:3623066/150‑1 (MQ=255)
                                                       cTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGt                                                                                          >  1:662652/1‑150 (MQ=255)
                                                        tGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGtt                                                                                         <  2:2033493/150‑1 (MQ=255)
                                                           gTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACCTGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGc                                                                                      <  1:5078457/150‑1 (MQ=255)
                                                           gTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGc                                                                                      >  1:538950/1‑150 (MQ=255)
                                                              aGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACTTGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAAt                                                                                   <  1:1942428/150‑1 (MQ=255)
                                                              aGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAAt                                                                                   >  1:5246072/1‑150 (MQ=255)
                                                               gcgcCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATc                                                                                  <  1:1156087/150‑1 (MQ=255)
                                                                cgcCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCg                                                                                 <  1:4769896/150‑1 (MQ=255)
                                                                cgcCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCg                                                                                 <  2:653975/150‑1 (MQ=255)
                                                                cgcCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCg                                                                                 <  1:2906423/150‑1 (MQ=255)
                                                                        cAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGAt                                                                         <  2:3245648/150‑1 (MQ=255)
                                                                           cGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGtt                                                                      >  2:3758069/1‑150 (MQ=255)
                                                                               aCCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCAt                                                                  <  1:4806293/150‑1 (MQ=255)
                                                                                 cTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATaa                                                                >  1:1093692/1‑150 (MQ=255)
                                                                                   ccccGCGCGGTGATAACGTTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGTAACGCGTGCAGAAAACTCCCGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACg                                                              <  1:4936271/150‑1 (MQ=255)
                                                                                       gcgcGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTc                                                          <  2:1452146/150‑1 (MQ=255)
                                                                                       gcgcGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTAGGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTc                                                          <  1:1994525/150‑1 (MQ=255)
                                                                                         gcgGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCgg                                                        <  1:4432976/150‑1 (MQ=255)
                                                                                           ggTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCgggg                                                      <  1:5211846/150‑1 (MQ=255)
                                                                                             ttaTAACCGTTTTGTGCGCGTGAGCTGGTATGAAGCCCTCGAAATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGAt                                                    <  1:424248/148‑1 (MQ=255)
                                                                                             tGATAACCGTTTTGTGCGCGTGAGGTGGTATGACGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGAt                                                    <  1:2283218/150‑1 (MQ=255)
                                                                                             tGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGAt                                                    <  1:4532765/150‑1 (MQ=255)
                                                                                              gATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATg                                                   >  2:3913887/1‑150 (MQ=255)
                                                                                               aTAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGTCAATCGACGGGGATGTTCCATAAAGCTTCTTGGATga                                                  >  1:94488/1‑149 (MQ=255)
                                                                                               aTAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGc                                                  >  2:4035228/1‑150 (MQ=255)
                                                                                               aTAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGc                                                  >  2:4846379/1‑150 (MQ=255)
                                                                                               aTAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGc                                                  <  1:1572545/150‑1 (MQ=255)
                                                                                               aTAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGc                                                  >  2:3312541/1‑150 (MQ=255)
                                                                                                 aaCCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTg                                                >  1:3394551/1‑150 (MQ=255)
                                                                                                   ccGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGc                                              <  2:4118353/150‑1 (MQ=255)
                                                                                                    cGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCg                                             <  1:4097583/150‑1 (MQ=255)
                                                                                                     gTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGa                                            >  1:2236872/1‑150 (MQ=255)
                                                                                                       tttGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGaaa                                          <  2:255702/150‑1 (MQ=255)
                                                                                                         tgtgCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCACTCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGc                                        >  1:2879212/1‑150 (MQ=255)
                                                                                                          gtgCGCGTGAGCTGGGATGTAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCTGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCt                                       <  2:902264/150‑1 (MQ=255)
                                                                                                            gcgcGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTAt                                     >  1:855237/1‑150 (MQ=255)
                                                                                                             cgcgTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTAtt                                    <  2:3179088/150‑1 (MQ=255)
                                                                                                             cgcgTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTAtt                                    <  1:3088621/150‑1 (MQ=255)
                                                                                                              gcgTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTg                                   <  1:3184867/150‑1 (MQ=255)
                                                                                                                 tGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCt                                >  1:2658484/1‑150 (MQ=255)
                                                                                                                 tGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCt                                >  2:553490/1‑150 (MQ=255)
                                                                                                                 tGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCt                                >  2:1676278/1‑150 (MQ=255)
                                                                                                                  gAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCtt                               <  2:3655503/150‑1 (MQ=255)
                                                                                                                   aGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTg                              <  2:1941416/150‑1 (MQ=255)
                                                                                                                    gCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGc                             >  2:4405514/1‑150 (MQ=255)
                                                                                                                      tGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCAt                           >  1:74434/1‑150 (MQ=255)
                                                                                                                      tGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCAt                           <  2:115001/150‑1 (MQ=255)
                                                                                                                          aTGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGAAGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATAGCCTTGCATGGTa                       >  1:1031076/1‑150 (MQ=255)
                                                                                                                           tGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTaa                      >  1:3148999/1‑150 (MQ=255)
                                                                                                                           tGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTaa                      >  1:1247885/1‑150 (MQ=255)
                                                                                                                            gAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAAt                     <  2:4138975/150‑1 (MQ=255)
                                                                                                                             aaGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATa                    >  1:3959609/1‑150 (MQ=255)
                                                                                                                             aaGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATa                    <  1:35128/150‑1 (MQ=255)
                                                                                                                              aGCCCTCGACAGGTTCTCTGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATAg                   <  2:4931648/150‑1 (MQ=255)
                                                                                                                                cccTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATAGCg                 >  2:354358/1‑150 (MQ=255)
                                                                                                                                        aTGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATAGCGTTGGTACg         >  2:1320374/1‑150 (MQ=255)
                                                                                                                                             cTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGTGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATAGCGTTGGTCCGGGCgg    >  2:1231433/1‑150 (MQ=255)
                                                                                                                                               aTGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATAGCGTTGGTACGGGCGgag  <  2:2688913/150‑1 (MQ=255)
                                                                                                                                               aTGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATAGCGTTGGTACGGGCGgag  <  1:4590783/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GGATCACGTACACAACGCGGCGCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAAGAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATAGCGTTGGTACGGGCGGAG  >  CP009273/1054984‑1055276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGATCACGTACACAACGCGGCGCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAAGAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATAGCGTTGGTACGGGCGGAGATTAC  >  CP009273/1054983‑1055281
                                                                                                                                                     |                                                                                                                                                     
CGGATCACGTACACAACGCGGCGCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAAT                                                                                                                                                       <  SRR19973062.3285958/150‑1 (MQ=60)
CGGATCACGTACACAACGCGGCGCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAAT                                                                                                                                                       >  SRR19973062.4123563/1‑150 (MQ=60)
 GGATCACGTACACAACGCGGCGCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTCTGAATA                                                                                                                                                      >  SRR19973062.2810760/1‑150 (MQ=60)
 GGATCACGTACACAACGCGGCGCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATA                                                                                                                                                      >  SRR19973062.3409863/1‑150 (MQ=60)
 GGATCACGTACACAACGCGGCGCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATA                                                                                                                                                      >  SRR19973062.2810803/1‑150 (MQ=60)
 GGATCACGTACACAACGCGGCGCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAAGA                                                                                                                                                      >  SRR19973062.680807/1‑150 (MQ=60)
  GATCACGTACACAACGCGGCGCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAA                                                                                                                                                     >  SRR19973062.86832/1‑150 (MQ=60)
   ATCACGTACACAACGCGGCGCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAAC                                                                                                                                                    <  SRR19973062.3348535/150‑1 (MQ=60)
    TCACGTACACAACGCGGCGCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGACAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACT                                                                                                                                                   <  SRR19973062.2516505/150‑1 (MQ=60)
     CACGTACACAACGCGGCGCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTG                                                                                                                                                  >  SRR19973062.3328369/1‑150 (MQ=60)
      ACGTACACAACGCGGCGCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGG                                                                                                                                                 >  SRR19973062.890285/1‑150 (MQ=60)
      ACGTACACAACGCGGCGCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGG                                                                                                                                                 >  SRR19973062.890204/1‑150 (MQ=60)
       CGTACACAACGCGGCGCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGA                                                                                                                                                <  SRR19973062.4883183/150‑1 (MQ=60)
        GTACACAACGCGGCGCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAA                                                                                                                                               >  SRR19973062.4160418/1‑150 (MQ=60)
        GTACACAACGCGGCGCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAA                                                                                                                                               >  SRR19973062.3165555/1‑150 (MQ=60)
          ACACAACGCGGCGCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACG                                                                                                                                             >  SRR19973062.2591814/1‑150 (MQ=60)
           CACAACGCGGCGCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGC                                                                                                                                            >  SRR19973062.2118276/1‑150 (MQ=60)
             CAACGCGGCGCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGT                                                                                                                                          <  SRR19973062.5260616/150‑1 (MQ=60)
                   GGCGCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAA                                                                                                                                    >  SRR19973062.1448100/1‑150 (MQ=60)
                     CGCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAAGAACTGGAACGCGTGCAGAAAA                                                                                                                                  >  SRR19973062.4629816/1‑150 (MQ=60)
                        GTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTC                                                                                                                               >  SRR19973062.4141420/1‑150 (MQ=60)
                         TATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCA                                                                                                                              <  SRR19973062.4279737/150‑1 (MQ=60)
                         TATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCA                                                                                                                              <  SRR19973062.4279738/150‑1 (MQ=60)
                         TATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCA                                                                                                                              <  SRR19973062.2318919/150‑1 (MQ=60)
                          ATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCAC                                                                                                                             <  SRR19973062.1153369/150‑1 (MQ=60)
                           TTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACG                                                                                                                            <  SRR19973062.3363269/150‑1 (MQ=60)
                           TTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACG                                                                                                                            >  SRR19973062.1488581/1‑150 (MQ=60)
                            TCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGG                                                                                                                           >  SRR19973062.3110732/1‑150 (MQ=60)
                             CGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGG                                                                                                                          <  SRR19973062.5178491/150‑1 (MQ=60)
                              GTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGC                                                                                                                         <  SRR19973062.3874503/150‑1 (MQ=60)
                              GTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGC                                                                                                                         <  SRR19973062.4496846/150‑1 (MQ=60)
                                 ATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGA                                                                                                                      >  SRR19973062.3276830/1‑150 (MQ=60)
                                  TCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAG                                                                                                                     <  SRR19973062.4701958/150‑1 (MQ=60)
                                  TCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAG                                                                                                                     >  SRR19973062.2327377/1‑150 (MQ=60)
                                   CCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGT                                                                                                                    >  SRR19973062.3633216/1‑150 (MQ=60)
                                       TGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCT                                                                                                                <  SRR19973062.3267603/150‑1 (MQ=60)
                                        GGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTT                                                                                                               <  SRR19973062.718660/150‑1 (MQ=60)
                                        GGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTT                                                                                                               >  SRR19973062.5209202/1‑150 (MQ=60)
                                         GTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTG                                                                                                              <  SRR19973062.1238157/150‑1 (MQ=60)
                                         GTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTG                                                                                                              <  SRR19973062.914969/150‑1 (MQ=60)
                                            CGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTG                                                                                                           <  SRR19973062.1984233/150‑1 (MQ=60)
                                                TGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCG                                                                                                       <  SRR19973062.295995/150‑1 (MQ=60)
                                                 GGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGC                                                                                                      >  SRR19973062.4898671/1‑150 (MQ=60)
                                                      GGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTG                                                                                                 <  SRR19973062.3641575/150‑1 (MQ=60)
                                                       GCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGG                                                                                                <  SRR19973062.3247717/150‑1 (MQ=60)
                                                        CTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGT                                                                                               >  SRR19973062.665735/1‑150 (MQ=60)
                                                            GTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACCTGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGC                                                                                           <  SRR19973062.5105352/150‑1 (MQ=60)
                                                            GTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGC                                                                                           >  SRR19973062.541489/1‑150 (MQ=60)
                                                               AGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACTTGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAAT                                                                                        <  SRR19973062.1952398/150‑1 (MQ=60)
                                                               AGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAAT                                                                                        >  SRR19973062.5273701/1‑150 (MQ=60)
                                                                GCTCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATC                                                                                       <  SRR19973062.4982781/150‑1 (MQ=60)
                                                                GCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATC                                                                                       <  SRR19973062.1161511/150‑1 (MQ=60)
                                                                 CGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCG                                                                                      <  SRR19973062.2921754/150‑1 (MQ=60)
                                                                 CGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCG                                                                                      <  SRR19973062.4794688/150‑1 (MQ=60)
                                                                 CGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCG                                                                                      <  SRR19973062.732105/150‑1 (MQ=60)
                                                                    CATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACG                                                                                   >  SRR19973062.3556535/1‑150 (MQ=60)
                                                                    CATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACG                                                                                   >  SRR19973062.1070816/1‑150 (MQ=60)
                                                                        TCCGCGATACCTCCCCGCGGGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGACCGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGA                                                                               <  SRR19973062.4080739/150‑1 (MQ=60)
                                                                        TCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGA                                                                               <  SRR19973062.794927/150‑1 (MQ=60)
                                                                            CGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTT                                                                           >  SRR19973062.2328836/1‑150 (MQ=60)
                                                                                ACCTCCCTGCGCGGGGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACTTGTTCTATGAATACCTGGAACGCGTGCTGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGGGGTTGGCAATCGACGGGGATGTTCCAT                                                                       <  SRR19973062.2166660/150‑1 (MQ=60)
                                                                                ACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCAT                                                                       <  SRR19973062.992014/150‑1 (MQ=60)
                                                                                ACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCAT                                                                       <  SRR19973062.4831207/150‑1 (MQ=60)
                                                                                  CTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAA                                                                     >  SRR19973062.1098840/1‑150 (MQ=60)
                                                                                    CCCCGCGCGGTGATAACGTTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGTAACGCGTGCAGAAAACTCCCGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACG                                                                   <  SRR19973062.4962087/150‑1 (MQ=60)
                                                                                      CCGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGTATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCT                                                                 <  SRR19973062.936554/150‑1 (MQ=60)
                                                                                        GCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTAGGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTC                                                               <  SRR19973062.2004786/150‑1 (MQ=60)
                                                                                          GCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGG                                                             <  SRR19973062.4455603/150‑1 (MQ=60)
                                                                                           CGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGG                                                            <  SRR19973062.1964862/150‑1 (MQ=60)
                                                                                            GGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGG                                                           <  SRR19973062.5239337/150‑1 (MQ=60)
                                                                                              TTATAACCGTTTTGTGCGCGTGAGCTGGTATGAAGCCCTCGAAATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGAT                                                         <  SRR19973062.426071/150‑1 (MQ=60)
                                                                                              TGATAACCGTTTTGTGCGCGTGAGGTGGTATGACGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGAT                                                         <  SRR19973062.2294983/150‑1 (MQ=60)
                                                                                              TGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGAT                                                         <  SRR19973062.4556353/150‑1 (MQ=60)
                                                                                                ATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGTCAATCGACGGGGATGTTCCATAAAGCTTCttggatga                                                       >  SRR19973062.95105/1‑142 (MQ=60)
                                                                                                ATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGC                                                       <  SRR19973062.1580322/150‑1 (MQ=60)
                                                                                                ATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGC                                                       >  SRR19973062.2620324/1‑150 (MQ=60)
                                                                                                ATAACCGTATTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCTCCAGTGGTTGGCAATCGACGGGGAAGTTCCATAACGCTTCGGGGATGC                                                       >  SRR19973062.3009554/1‑150 (MQ=60)
                                                                                                  AACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTG                                                     <  SRR19973062.5327353/150‑1 (MQ=60)
                                                                                                  AACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTG                                                     >  SRR19973062.3411968/1‑150 (MQ=60)
                                                                                                  AACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAAGAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTG                                                     >  SRR19973062.1814334/1‑150 (MQ=60)
                                                                                                     CGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCG                                                  <  SRR19973062.4118705/150‑1 (MQ=60)
                                                                                                     CGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCG                                                  <  SRR19973062.4118512/150‑1 (MQ=60)
                                                                                                     CGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCG                                                  <  SRR19973062.1301844/150‑1 (MQ=60)
                                                                                                      GTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGA                                                 >  SRR19973062.2921024/1‑150 (MQ=60)
                                                                                                      GTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGA                                                 >  SRR19973062.2248048/1‑150 (MQ=60)
                                                                                                       TTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAA                                                >  SRR19973062.427228/1‑150 (MQ=60)
                                                                                                          TGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCACTCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGC                                             >  SRR19973062.2894425/1‑150 (MQ=60)
                                                                                                           GTGCGCGTGTGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCCCGGGCCGAGTGCATTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCT                                            <  SRR19973062.894967/150‑1 (MQ=60)
                                                                                                           GTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCT                                            <  SRR19973062.4184152/150‑1 (MQ=60)
                                                                                                             GCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTAT                                          >  SRR19973062.858926/1‑150 (MQ=60)
                                                                                                              CGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATT                                         <  SRR19973062.3104573/150‑1 (MQ=60)
                                                                                                               GCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTG                                        <  SRR19973062.3201388/150‑1 (MQ=60)
                                                                                                                  TGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCT                                     <  SRR19973062.2096306/150‑1 (MQ=60)
                                                                                                                  TGAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCT                                     >  SRR19973062.2672441/1‑150 (MQ=60)
                                                                                                                   GAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTT                                    <  SRR19973062.5119923/150‑1 (MQ=60)
                                                                                                                   GAGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTT                                    >  SRR19973062.5232745/1‑150 (MQ=60)
                                                                                                                    AGCTGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTG                                   <  SRR19973062.2601672/150‑1 (MQ=60)
                                                                                                                       TGGGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCAT                                >  SRR19973062.74930/1‑150 (MQ=60)
                                                                                                                         GGATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGG                              >  SRR19973062.4331853/1‑150 (MQ=60)
                                                                                                                           ATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTA                            >  SRR19973062.71580/1‑150 (MQ=60)
                                                                                                                           ATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTA                            >  SRR19973062.2292893/1‑150 (MQ=60)
                                                                                                                           ATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTA                            >  SRR19973062.3926249/1‑150 (MQ=60)
                                                                                                                           ATGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGAAGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATAGCCTTGCATGGTA                            >  SRR19973062.1035905/1‑150 (MQ=60)
                                                                                                                            TGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAA                           >  SRR19973062.1253656/1‑150 (MQ=60)
                                                                                                                            TGAAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAA                           >  SRR19973062.3165254/1‑150 (MQ=60)
                                                                                                                              AAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATA                         >  SRR19973062.3979692/1‑150 (MQ=60)
                                                                                                                              AAGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATA                         <  SRR19973062.35379/150‑1 (MQ=60)
                                                                                                                               AGCCCTCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATAG                        <  SRR19973062.4797187/150‑1 (MQ=60)
                                                                                                                                 CCCTCGACTTGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATAGCG                      <  SRR19973062.655585/150‑1 (MQ=60)
                                                                                                                                    TCGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATAGCGTTG                   <  SRR19973062.4575214/150‑1 (MQ=60)
                                                                                                                                     CGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATAGCGTTGG                  >  SRR19973062.3524211/1‑150 (MQ=60)
                                                                                                                                     CGACATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATAGCGTTGG                  <  SRR19973062.3725213/150‑1 (MQ=60)
                                                                                                                                         ATGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATAGCGTTGGTACG              >  SRR19973062.4648890/1‑150 (MQ=60)
                                                                                                                                          TGTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATAGCGTTGGTACGG             >  SRR19973062.2794175/1‑150 (MQ=60)
                                                                                                                                           GTTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATAGCGTTGGTACGGG            >  SRR19973062.4772446/1‑150 (MQ=60)
                                                                                                                                            TTCTATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATAGCGTTGGTACGGGC           <  SRR19973062.2541236/150‑1 (MQ=60)
                                                                                                                                                ATGAATAACTGGAACGCGTGCAGAAAACTCCCGGGCCGCGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATAGCGTTGGTACGGGAGGAG       <  SRR19973062.2265957/150‑1 (MQ=60)
                                                                                                                                                ATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATAGCGTTGGTACGGGCGGAG       <  SRR19973062.4614793/150‑1 (MQ=60)
                                                                                                                                                ATGAATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATAGCGTTGGTACGGGCGGAG       >  SRR19973062.1439439/1‑150 (MQ=60)
                                                                                                                                                    ATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATAGCGTTGGTACGGGCGGAGATTA   >  SRR19973062.4590889/1‑150 (MQ=60)
                                                                                                                                                    ATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATAGCGTTGGTACGGGCGGAGATTA   >  SRR19973062.2827518/1‑150 (MQ=60)
                                                                                                                                                    ATAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATAGCGTTGGTACGGGCGGAGATTA   <  SRR19973062.566742/150‑1 (MQ=60)
                                                                                                                                                     TAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATAGCGTTGGTACGGGCGGAGATTAC  <  SRR19973062.2655142/150‑1 (MQ=60)
                                                                                                                                                     TAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATAGCGTTGGTACGGGCGGAGATTAC  <  SRR19973062.2655095/150‑1 (MQ=60)
                                                                                                                                                     TAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATCGTAATAGCGTTGGTACGGGCGGAGATTAC  >  SRR19973062.4354126/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CGGATCACGTACACAACGCGGCGCGTATTCGTTATCCGATGGTACGCGTGGACTGGCTGCGTAAGCGCCATCTCAGCGATACCTCCCAGCGCGGTGATAACCGTTTTGTGCGCGTGAGCTGGGATGAAGCCCTCGACATGTTCTATGAAGAACTGGAACGCGTGCAGAAAACTCACGGGCCGAGTGCCTTGCTGACCGCCAGTGGTTGGCAATCGACGGGGATGTTCCATAACGCTTCGGGGATGCTGGCGAAAGCTATTGCCTTGCATGGTAATAGCGTTGGTACGGGCGGAGATTAC  >  CP009273/1054983‑1055281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑