Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,067,754 T→A 38.9% H189L (CAC→CTC)  rutB ← ureidoacrylate amidohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,067,7540TA38.9% 268.9 / inf 316H189L (CAC→CTC) rutBureidoacrylate amidohydrolase
Reads supporting (aligned to +/- strand):  ref base T (117/76);  new base A (69/54);  total (186/130)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 317 total aligned reads displayed.

GATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATGGCGTATTCCGCGGCTGCGCAAAATGCTGTCCAGCGGCGTATTGAA  >  CP009273/1067605‑1067902
                                                                                                                                                     |                                                                                                                                                    
  ttttGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGa                                                                                                                                                    <  2:2060874/150‑1 (MQ=255)
   tttGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAg                                                                                                                                                   <  2:4602263/150‑1 (MQ=255)
    ttGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGt                                                                                                                                                  <  2:3147930/150‑1 (MQ=255)
      ggCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTg                                                                                                                                                >  1:3375540/1‑150 (MQ=255)
      ggCATCGTTAAACTCATTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTg                                                                                                                                                <  2:4521431/150‑1 (MQ=255)
            gttcaccTCCGTACGCGCTATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAAGGTGTCGCCGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGCACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCtt                                                                                                                                          <  2:3870665/144‑1 (MQ=255)
            gTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCtt                                                                                                                                          >  2:4876879/1‑150 (MQ=255)
            gTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCtt                                                                                                                                          >  1:270436/1‑150 (MQ=255)
             ttAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTc                                                                                                                                         <  1:2659635/150‑1 (MQ=255)
               aaaCTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAGGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCaa                                                                                                                                       <  1:2591987/150‑1 (MQ=255)
                   tCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGcac                                                                                                                                   <  2:1030335/150‑1 (MQ=255)
                     cTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGcacca                                                                                                                                 >  1:4968917/1‑150 (MQ=255)
                      ttAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGcaccac                                                                                                                                <  1:2796122/150‑1 (MQ=255)
                      ttAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGcaccac                                                                                                                                <  1:5227400/150‑1 (MQ=255)
                            gATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGaa                                                                                                                          >  1:2326611/1‑150 (MQ=255)
                             atatGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGaaa                                                                                                                         <  1:4940893/150‑1 (MQ=255)
                                tGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATAc                                                                                                                      <  2:1828445/150‑1 (MQ=255)
                                 gAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACt                                                                                                                     <  1:3457108/150‑1 (MQ=255)
                                  aGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTc                                                                                                                    >  1:1670384/1‑150 (MQ=255)
                                   gCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCC‑AAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCa                                                                                                                  <  2:2707350/150‑1 (MQ=255)
                                    cAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCa                                                                                                                  <  1:3964337/150‑1 (MQ=255)
                                     aaaGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAg                                                                                                                 >  2:4052803/1‑150 (MQ=255)
                                     aaaGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAg                                                                                                                 >  1:898873/1‑150 (MQ=255)
                                      aaGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGa                                                                                                                >  2:5176384/1‑150 (MQ=255)
                                      aaGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGa                                                                                                                >  1:3810593/1‑150 (MQ=255)
                                      aaGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGa                                                                                                                >  2:712960/1‑150 (MQ=255)
                                          aCGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGaaaaa                                                                                                            >  2:5263077/1‑150 (MQ=255)
                                            gTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAg                                                                                                          <  1:4018806/150‑1 (MQ=255)
                                              gggAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCAAGCAAAAAAAGGTTTCGATATTGAACAACTCGGCTTTCTGCGAAAATTTCGGCCCCTCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCATAAAAAAGca                                                                                                        >  2:3613205/1‑149 (MQ=255)
                                               ggAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGACATACTCCAGAAAAAAGCCg                                                                                                       >  2:2876619/1‑150 (MQ=255)
                                                gagaAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGt                                                                                                      <  2:3387792/150‑1 (MQ=255)
                                                  gaAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTcg                                                                                                    >  2:399828/1‑150 (MQ=255)
                                                   aaaGCGCGTCGGAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAACTACTCCAGACAATAGCCGgcgc                                                                                                   >  1:2768886/1‑150 (MQ=255)
                                                   aaaGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTcgc                                                                                                   >  1:4308476/1‑150 (MQ=255)
                                                       cgcgTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAg                                                                                               <  2:152363/150‑1 (MQ=255)
                                                        gcgTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGc                                                                                              <  1:2374495/150‑1 (MQ=255)
                                                            cGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCg                                                                                          >  2:632766/1‑150 (MQ=255)
                                                               aGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGAtt                                                                                       >  2:2417241/1‑150 (MQ=255)
                                                                  aTGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGa                                                                                    >  2:2140446/1‑150 (MQ=255)
                                                                     tttCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGc                                                                                 >  1:1855000/1‑150 (MQ=255)
                                                                       tCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAg                                                                               >  2:1465395/1‑150 (MQ=255)
                                                                       tCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAg                                                                               >  1:1760825/1‑150 (MQ=255)
                                                                        cGACGTCGCTGA‑CCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGAc                                                                             >  1:224999/1‑150 (MQ=255)
                                                                           cGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGt                                                                           >  1:649725/1‑150 (MQ=255)
                                                                            gTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGtt                                                                          <  2:5040105/150‑1 (MQ=255)
                                                                            gTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGtt                                                                          >  1:438365/1‑150 (MQ=255)
                                                                            gTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGtt                                                                          >  2:1223355/1‑150 (MQ=255)
                                                                              cGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTgg                                                                        >  1:3216275/1‑150 (MQ=255)
                                                                                cTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTa                                                                      <  1:5322767/150‑1 (MQ=255)
                                                                                cTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTa                                                                      <  1:3124238/150‑1 (MQ=255)
                                                                                 tGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAg                                                                     >  2:2360813/1‑150 (MQ=255)
                                                                                  gACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGc                                                                    <  2:3284626/150‑1 (MQ=255)
                                                                                   aCCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCg                                                                   >  2:431818/1‑150 (MQ=255)
                                                                                   aCCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCg                                                                   >  2:1266004/1‑150 (MQ=255)
                                                                                     ccAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGAt                                                                 <  1:1686709/150‑1 (MQ=255)
                                                                                        gCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGcc                                                              <  1:2854356/150‑1 (MQ=255)
                                                                                            aaaaaGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGACAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTg                                                          >  1:1989141/1‑150 (MQ=255)
                                                                                            aaaaaGGTATCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTg                                                          <  1:4566216/150‑1 (MQ=255)
                                                                                             aaaaGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGa                                                         >  1:1271625/1‑150 (MQ=255)
                                                                                             aaaaGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGa                                                         >  2:334696/1‑150 (MQ=255)
                                                                                                aGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGaaaa                                                      >  2:1895771/1‑150 (MQ=255)
                                                                                                 ggTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAAc                                                     >  1:216185/1‑150 (MQ=255)
                                                                                                  gTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAAcc                                                    >  1:1491641/1‑150 (MQ=255)
                                                                                                   tttCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCa                                                   <  1:1359759/150‑1 (MQ=255)
                                                                                                   tttCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCa                                                   <  2:4853478/150‑1 (MQ=255)
                                                                                                    ttCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAg                                                  <  2:1622843/150‑1 (MQ=255)
                                                                                                     tCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGa                                                 <  1:3955459/150‑1 (MQ=255)
                                                                                                       gATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATg                                               >  1:4416519/1‑150 (MQ=255)
                                                                                                       gATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATg                                               >  2:2478738/1‑150 (MQ=255)
                                                                                                       gATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATg                                               <  1:5142571/150‑1 (MQ=255)
                                                                                                        atatTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATgg                                              >  1:1117965/1‑150 (MQ=255)
                                                                                                        atatTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATgg                                              >  2:4713995/1‑150 (MQ=255)
                                                                                                        atatTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATgg                                              <  2:1391359/150‑1 (MQ=255)
                                                                                                         tatTGAACAACGCGGCTTTCTGCGCAAATTTCTGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATGGc                                             >  2:3937243/1‑150 (MQ=255)
                                                                                                          atTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATGGCg                                            >  1:2920261/1‑150 (MQ=255)
                                                                                                              aacaacGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATGGCGTAtt                                        >  1:4624555/1‑150 (MQ=255)
                                                                                                                caacGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATGGCGTATTcc                                      <  2:4408104/150‑1 (MQ=255)
                                                                                                                  acGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATGGCGTATTCcgc                                    <  2:898873/150‑1 (MQ=255)
                                                                                                                  acGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATGGCGTATTCcgc                                    <  2:3780282/150‑1 (MQ=255)
                                                                                                                      ggCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATGGCGTATTCCGCGGCt                                <  2:4122808/150‑1 (MQ=255)
                                                                                                                      ggCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATGGCGTATTCCGCGGCt                                >  2:3680102/1‑150 (MQ=255)
                                                                                                                        cTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATGGCGTATTCCGCGGCTgc                              >  1:4775627/1‑150 (MQ=255)
                                                                                                                        cTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATGGCGTATTCCGCGGCTgc                              <  2:2773825/150‑1 (MQ=255)
                                                                                                                        cTTTCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATGGCGTATTCCGCGGCTgc                              <  2:1813055/150‑1 (MQ=255)
                                                                                                                           tCTGCGCAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCATGCACCACGCCGAAATACTCCAGAAAATAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATGGCGTATTCCGCGGCTGCGCa                           <  2:2212400/150‑1 (MQ=255)
                                                                                                                              gcgcAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATGGCGTATTCCGCGGCTGCGCaaaa                        >  1:3027594/1‑150 (MQ=255)
                                                                                                                               cgcAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATGGCGTATTCCGCGGCTGCGCAAAAt                       <  1:1236222/150‑1 (MQ=255)
                                                                                                                               cgcAAATTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATGGCGTATTCCGCGGCTGCGCAAAAt                       <  2:748443/150‑1 (MQ=255)
                                                                                                                                  aaaTTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGGGGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATGGCGTATTCCGCGGCTGCGCAAAATGCt                    <  2:4209010/150‑1 (MQ=255)
                                                                                                                                  aaaTTTCGGCCCCGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATGGCGTATTCCGCGGCTGCGCAAAATGCt                    >  1:1006747/1‑150 (MQ=255)
                                                                                                                                       tCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATGGCGTATTCCGCGGCTGCGCAAAATGCTGTCCa               >  1:560536/1‑150 (MQ=255)
                                                                                                                                        cGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATGGCGTATTCCGCGGCTGCGCAAAATGCTGTCCAg              >  1:1060840/1‑150 (MQ=255)
                                                                                                                                        cGGCCCCGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATGGCGTATTCCGCGGCTGCGCAAAATGCTGTCCAg              <  1:3762544/150‑1 (MQ=255)
                                                                                                                                         ggCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAAACAGATGGCGTATTCCGCGGCTGCGCAAAATGCTGTCCAgc             >  1:4701064/1‑150 (MQ=255)
                                                                                                                                           ccccGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATGGCGTATTCCGCGGCTGCGCAAAATGCTGTCCAgcgg           >  2:5223785/1‑150 (MQ=255)
                                                                                                                                           ccccGCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATGGCGTATTCCGCGGCTGCGCAAAATGCTGTCCAgcgg           >  1:1701223/1‑150 (MQ=255)
                                                                                                                                            cccGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATGGCGTATTCCGCGGCTGCGCAAAATGCTGTCCAgcggc          <  1:4175769/150‑1 (MQ=255)
                                                                                                                                               gCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATGGCGTATTCCGCGGCTGCGCAAAATGCTGTCCAGCGGCGTa       >  1:791599/1‑150 (MQ=255)
                                                                                                                                               gCCTGGAGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATGGCGTATTCCGCGGCTGCGCAAACTGCTGTCCAGCGGCGTa       <  2:591309/150‑1 (MQ=255)
                                                                                                                                                ccTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATGGCGTATTCCGCGGCTGCGCAAAATGCTGTCCAGCGGCGTAt      >  1:4058082/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGTAGCGATGCCGGTGAAAACCAGATGGCGTATTCCGCGGCTGCGCAAAATGCTGTCCAGCGGCGTATTGAA  >  CP009273/1067605‑1067902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A