Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,326,726 A→T 27.2% P474P (CCA→CCT)  topA → DNA topoisomerase I, omega subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,326,7260AT27.2% 527.7 / 240.0 305P474P (CCA→CCT) topADNA topoisomerase I, omega subunit
Reads supporting (aligned to +/- strand):  ref base A (108/114);  new base T (48/35);  total (156/149)
Fisher's exact test for biased strand distribution p-value = 1.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 312 total aligned reads displayed.

CGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGA  >  CP009273/1326577‑1326875
                                                                                                                                                     |                                                                                                                                                     
  cgGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGc                                                                                                                                                     >  1:5066309/1‑150 (MQ=255)
      cGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGt                                                                                                                                                 <  1:1115777/150‑1 (MQ=255)
       gTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGtt                                                                                                                                                >  2:413152/1‑150 (MQ=255)
        tATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCTGCCCGttt                                                                                                                                               <  2:5121619/150‑1 (MQ=255)
        tATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGttt                                                                                                                                               >  2:762868/1‑150 (MQ=255)
        tATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGttt                                                                                                                                               <  2:3146315/150‑1 (MQ=255)
        tATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGttt                                                                                                                                               <  1:4205503/150‑1 (MQ=255)
            ttGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGt                                                                                                                                           >  2:896840/1‑150 (MQ=255)
            ttGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGt                                                                                                                                           >  2:3572305/1‑150 (MQ=255)
               cGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGaa                                                                                                                                        >  1:77338/1‑150 (MQ=255)
               cGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGaa                                                                                                                                        >  1:3500914/1‑150 (MQ=255)
                gTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAg                                                                                                                                       >  1:4493452/1‑150 (MQ=255)
                   ttGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCTGCCCGTTTCAGTGAAGCAt                                                                                                                                    <  2:1098223/150‑1 (MQ=255)
                   ttGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCTGCCCGTTTAAGTGAAGCAt                                                                                                                                    <  1:1838363/150‑1 (MQ=255)
                       tGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCt                                                                                                                                >  2:4486038/1‑150 (MQ=255)
                        ggCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCTGCCCGTTTCAGTGACGCATCGCTg                                                                                                                               >  2:504122/1‑150 (MQ=255)
                          cTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGt                                                                                                                             <  1:200504/150‑1 (MQ=255)
                           tGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCTGCCCGTTTCAGTGAAGCATCGCTGGtt                                                                                                                            <  1:3952422/150‑1 (MQ=255)
                           tGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGtt                                                                                                                            >  2:2181365/1‑150 (MQ=255)
                             gACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGAGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCTGCCCGTTTCAGTGAAGCATCGCTGGTTaa                                                                                                                          >  2:1826914/1‑150 (MQ=255)
                               cAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCTGCCCGTTTCAGTGAAGCATCGCTGGTTAAag                                                                                                                        >  1:2113431/1‑150 (MQ=255)
                                aaaaGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCTGCCCGTTTCAGTGAAGCATCGCTGGTTAAaga                                                                                                                       >  1:5210326/1‑150 (MQ=255)
                                aaaaGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCTGCCCGTTTCAGTGAAGCATCGCTGGTTAAaga                                                                                                                       >  1:1726827/1‑150 (MQ=255)
                                 aaaGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCTGCCCGTTTCAGTGAAGCATCGCTGGTTAAagag                                                                                                                      <  2:4530812/150‑1 (MQ=255)
                                 aaaGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAagag                                                                                                                      >  1:1943171/1‑150 (MQ=255)
                                 aaaGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAagag                                                                                                                      <  2:78293/150‑1 (MQ=255)
                                  aaGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGc                                                                                                                     <  1:3699848/150‑1 (MQ=255)
                                   aGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCt                                                                                                                    >  1:2216408/1‑150 (MQ=255)
                                    gTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTg                                                                                                                   >  1:2405534/1‑150 (MQ=255)
                                    gTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTg                                                                                                                   >  1:5034416/1‑150 (MQ=255)
                                     tGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCTGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTgg                                                                                                                  >  2:5261795/1‑150 (MQ=255)
                                      gATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCTGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGa                                                                                                                 <  2:136114/150‑1 (MQ=255)
                                          ccTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCTGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGaaaaa                                                                                                             >  1:2534566/1‑150 (MQ=255)
                                           cTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAAc                                                                                                            <  2:4160301/150‑1 (MQ=255)
                                               gttgcgtAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCTGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCgg                                                                                                        >  1:4888329/1‑150 (MQ=255)
                                                ttgcgtAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGt                                                                                                       >  1:4286380/1‑150 (MQ=255)
                                                   cgtAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCTGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATc                                                                                                    <  1:4052232/150‑1 (MQ=255)
                                                    gtAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCTGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCg                                                                                                   <  1:3257979/150‑1 (MQ=255)
                                                    gtAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCTGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCg                                                                                                   <  1:5183307/150‑1 (MQ=255)
                                                    gtAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCg                                                                                                   <  2:4990001/150‑1 (MQ=255)
                                                     tAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCTGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCgg                                                                                                  >  2:4717068/1‑150 (MQ=255)
                                                      aaaGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCTGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGgt                                                                                                 <  2:1464172/150‑1 (MQ=255)
                                                       aaGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGgtc                                                                                                >  1:4391824/1‑150 (MQ=255)
                                                            gATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCTGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTcgtccg                                                                                           >  2:4860392/1‑150 (MQ=255)
                                                             aTGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTcgtccgt                                                                                          >  2:2604318/1‑150 (MQ=255)
                                                                aaGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTa                                                                                       <  2:3243330/150‑1 (MQ=255)
                                                                aaGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTa                                                                                       <  1:2094184/150‑1 (MQ=255)
                                                                 aGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTAc                                                                                      >  1:4560695/1‑150 (MQ=255)
                                                                 aGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTAc                                                                                      >  1:2620292/1‑150 (MQ=255)
                                                                   aTCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCt                                                                                    <  1:96132/150‑1 (MQ=255)
                                                                    tCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTa                                                                                   >  1:1767054/1‑150 (MQ=255)
                                                                     cGCATATTACCAGAAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTAt                                                                                  <  2:870331/150‑1 (MQ=255)
                                                                      gCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATg                                                                                 <  2:1138862/150‑1 (MQ=255)
                                                                        aTCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCg                                                                               <  2:2940445/150‑1 (MQ=255)
                                                                        aTCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCg                                                                               >  2:3112421/1‑150 (MQ=255)
                                                                        aTCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCg                                                                               >  2:2314222/1‑150 (MQ=255)
                                                                        aTCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCg                                                                               <  2:2022347/150‑1 (MQ=255)
                                                                          cTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTc                                                                             <  2:4718601/150‑1 (MQ=255)
                                                                          cTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTc                                                                             <  1:890733/150‑1 (MQ=255)
                                                                           ttACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCg                                                                            >  1:1365617/1‑150 (MQ=255)
                                                                                 gcagTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAATGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCAtt                                                                      <  1:2116595/150‑1 (MQ=255)
                                                                                   agTTAATACAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTc                                                                    <  1:57121/150‑1 (MQ=255)
                                                                                   agTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCTGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTc                                                                    >  1:4129306/1‑150 (MQ=255)
                                                                                   agTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCTGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTc                                                                    >  2:733295/1‑150 (MQ=255)
                                                                                   agTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTc                                                                    >  1:2332012/1‑150 (MQ=255)
                                                                                     ttAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGa                                                                  <  2:4583050/150‑1 (MQ=255)
                                                                                      taataaAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGAc                                                                 >  1:3354298/1‑150 (MQ=255)
                                                                                        ataaAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCa                                                               >  2:4082525/1‑150 (MQ=255)
                                                                                          aaaGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCAtt                                                             >  2:1285031/1‑150 (MQ=255)
                                                                                          aaaGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCAtt                                                             >  2:1203963/1‑150 (MQ=255)
                                                                                              gCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAgg                                                         <  2:586707/150‑1 (MQ=255)
                                                                                                 aTGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCTGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATc                                                      >  1:3202559/1‑150 (MQ=255)
                                                                                                  tGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCg                                                     <  2:2431858/150‑1 (MQ=255)
                                                                                                    ctctGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTg                                                   >  1:2756989/1‑150 (MQ=255)
                                                                                                              cGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCTGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCg                                         >  2:3061996/1‑150 (MQ=255)
                                                                                                              cGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCg                                         <  2:2712433/150‑1 (MQ=255)
                                                                                                              cGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCg                                         <  1:2892112/150‑1 (MQ=255)
                                                                                                              cGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCg                                         <  2:4324989/150‑1 (MQ=255)
                                                                                                               gTTGAACTTACACCAGCCCAGCACTTTACCATGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGa                                        >  1:42624/1‑150 (MQ=255)
                                                                                                               gTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCTGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGa                                        >  2:2247130/1‑150 (MQ=255)
                                                                                                               gTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCTGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGa                                        >  1:1608607/1‑150 (MQ=255)
                                                                                                                  gAACTTACACCAGCCCAGCACTTTACCAAGCCGCCTGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTa                                     >  1:1286944/1‑150 (MQ=255)
                                                                                                                   aaCTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAg                                    >  2:1635330/1‑150 (MQ=255)
                                                                                                                        acacCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAAt                               <  1:4733296/150‑1 (MQ=255)
                                                                                                                        acacCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAAt                               <  1:1187898/150‑1 (MQ=255)
                                                                                                                        acacCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAAt                               <  1:623680/150‑1 (MQ=255)
                                                                                                                            cagcccagcACTTTACCAAGCCGCCTGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAAtcgtc                           >  2:4035359/1‑150 (MQ=255)
                                                                                                                            cagcccagcACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAAtcgtc                           <  1:304664/150‑1 (MQ=255)
                                                                                                                                ccagcACTTTACCAAGCCGCCTGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGttt                       <  2:1324571/150‑1 (MQ=255)
                                                                                                                                ccagcACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGttt                       <  2:1685228/150‑1 (MQ=255)
                                                                                                                                 cagcACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTc                      <  2:4391824/150‑1 (MQ=255)
                                                                                                                                     aCTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATg                  <  1:1472560/150‑1 (MQ=255)
                                                                                                                                        ttACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATGCgg               <  1:3951746/150‑1 (MQ=255)
                                                                                                                                            cAAGCCGCCTGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGGCGTTTCTATGCGGcaaa           >  2:658027/1‑150 (MQ=255)
                                                                                                                                             aaGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGaaaaa          >  2:4946661/1‑150 (MQ=255)
                                                                                                                                              aGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGaaaaaa         >  2:3812801/1‑150 (MQ=255)
                                                                                                                                                 cgccAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATgg      <  1:3887588/150‑1 (MQ=255)
                                                                                                                                                   ccAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAACACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGc    <  2:47516/150‑1 (MQ=255)
                                                                                                                                                     aGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGa  >  1:850069/1‑150 (MQ=255)
                                                                                                                                                     aGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGGTTCTATGCGGAAAAAATGGGCGa  >  2:4241656/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGA  >  CP009273/1326577‑1326875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A