Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F527 I0 R1	1651	322.4	10661268	95.2%	10149527	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,310,875	A→T	26.7%	I827I (ATT→ATA)	rcsC ←	hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,310,875	0	A	T	26.7%	608.3 / 241.3	337	I827I (ATT→ATA)	rcsC	hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand): ref base A (136/110); new base T (43/48); total (179/158)
Fisher's exact test for biased strand distribution p-value = 2.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 340 total aligned reads displayed.

TGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACTCCCCTGG  >  CP009273/2310727‑2311024

 gACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAat                                                                                                                                                     >  2:1186690/1‑150 (MQ=255)

  aCGTCGCTAAGCACGATATCAATATGAGTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAatc                                                                                                                                                    <  2:2850536/150‑1 (MQ=255)

      cGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGTAtcatca                                                                                                                                                >  2:1871899/1‑150 (MQ=255)

       gCTAAGCACGATATCAATATTATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATcatcat                                                                                                                                               <  1:967741/150‑1 (MQ=255)

       gCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATcatcat                                                                                                                                               <  1:5298766/150‑1 (MQ=255)

       gCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATcatcat                                                                                                                                               <  2:3243638/150‑1 (MQ=255)

         tAAGCACGATATCAATATGATTCTTGCTGAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATACACGACCAGAATCATCatat                                                                                                                                             >  2:3528043/1‑150 (MQ=255)

         tAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCatat                                                                                                                                             >  1:1580627/1‑150 (MQ=255)

             cACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTc                                                                                                                                         >  1:1014996/1‑150 (MQ=255)

              aCGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCa                                                                                                                                        >  1:1887725/1‑150 (MQ=255)

              aCGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCa                                                                                                                                        <  2:4769302/150‑1 (MQ=255)

               cGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGTATCATCATATCGTCAt                                                                                                                                       <  1:3741779/150‑1 (MQ=255)

               cGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCag                                                                                                                                       <  2:958044/150‑2 (MQ=255)

               cGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCAt                                                                                                                                       <  2:598246/150‑1 (MQ=255)

                 atatCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTa                                                                                                                                     >  1:1636709/1‑150 (MQ=255)

                  tatCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGTATCATCATATCGTCATTAt                                                                                                                                    <  2:4684536/150‑1 (MQ=255)

                  tatCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTAt                                                                                                                                    <  1:1917332/150‑1 (MQ=255)

                      aaTATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCt                                                                                                                                >  1:3456660/1‑150 (MQ=255)

                         atGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGAc                                                                                                                             >  2:672709/1‑150 (MQ=255)

                          tGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGAcc                                                                                                                            >  1:119731/1‑150 (MQ=255)

                               cTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCttt                                                                                                                       <  2:1086322/150‑1 (MQ=255)

                               cTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCttt                                                                                                                       >  2:208843/1‑150 (MQ=255)

                               cTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCttt                                                                                                                       >  2:4713559/1‑150 (MQ=255)

                                   cTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGATAATCGGATGATCATCAACGACCAGAATCATCATATCGTCATAATCTCTGACAGCTTTGTcc                                                                                                                   >  1:4469466/1‑150 (MQ=255)

                                      aGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCACCGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGtt                                                                                                                <  1:651079/150‑1 (MQ=255)

                                      aGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGtt                                                                                                                <  2:3701598/150‑1 (MQ=255)

                                      aGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGtt                                                                                                                >  2:859341/1‑150 (MQ=255)

                                        tACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGa                                                                                                              <  2:5265151/150‑1 (MQ=255)

                                         aCATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGAc                                                                                                             <  2:5125074/150‑1 (MQ=255)

                                         aCATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCAGTATCGCTGACCGCTTTGTCCGTTGAc                                                                                                             >  2:3863773/1‑150 (MQ=255)

                                           aTTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGTATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACgg                                                                                                           >  2:2689100/1‑150 (MQ=255)

                                           aTTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACgg                                                                                                           >  1:536679/1‑150 (MQ=255)

                                               aGCGCATCGACGCCATCATTCGCGGTTTTACTTTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCaga                                                                                                       <  2:2315822/150‑1 (MQ=255)

                                                  gcATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGATGACGGCAGAGCg                                                                                                    >  1:4948270/1‑150 (MQ=255)

                                                   cATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGTATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGt                                                                                                   <  1:4509677/150‑1 (MQ=255)

                                                    aTCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGTATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGtt                                                                                                  >  1:904742/1‑150 (MQ=255)

                                                    aTCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGTATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGtt                                                                                                  <  1:3180174/150‑1 (MQ=255)

                                                     tCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTa                                                                                                 <  1:3893345/150‑1 (MQ=255)

                                                       gACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGc                                                                                               >  2:4960572/1‑150 (MQ=255)

                                                          gCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGTATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAgg                                                                                            >  1:3314086/1‑150 (MQ=255)

                                                          gCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAgg                                                                                            >  2:5070729/1‑150 (MQ=255)

                                                           ccATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGTATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGa                                                                                           >  2:4150027/1‑150 (MQ=255)

                                                            catcatTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGAt                                                                                          >  2:2600625/1‑150 (MQ=255)

                                                             atcatTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGAtc                                                                                         >  1:4171588/1‑150 (MQ=255)

                                                             atcatGCGGGGTTTTACTTTGATAGCCCACCGATCCCACCTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGTATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGAtc                                                                                         <  1:1357351/150‑1 (MQ=255)

                                                              tcatTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGAtcg                                                                                        >  1:2979128/1‑150 (MQ=255)

                                                                 ttCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGAtcgtcg                                                                                     <  1:2154539/150‑1 (MQ=255)

                                                                 ttCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGAtcgtcg                                                                                     <  1:2752843/150‑1 (MQ=255)

                                                                 ttCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGAAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGAtcgtcg                                                                                     >  1:4965102/1‑150 (MQ=255)

                                                                    gcgGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGTATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGctc                                                                                  <  1:4287192/150‑1 (MQ=255)

                                                                     cgGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGctct                                                                                 >  1:2846721/1‑150 (MQ=255)

                                                                      ggTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCtctc                                                                                >  1:3513961/1‑150 (MQ=255)

                                                                      ggTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCtctc                                                                                >  2:3748800/1‑150 (MQ=255)

                                                                       gTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTcc                                                                               >  1:2273335/1‑150 (MQ=255)

                                                                        ttttACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGTATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCa                                                                              <  1:4115094/150‑1 (MQ=255)

                                                                           tACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCAtct                                                                           >  2:2304200/1‑150 (MQ=255)

                                                                             cATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGATGACGGCAGAGCGTTAGCAGGATCGTCGCACTCCATCTCg                                                                         >  2:2539087/1‑150 (MQ=255)

                                                                              aTTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGa                                                                        >  2:150305/1‑150 (MQ=255)

                                                                                tGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATc                                                                      <  1:4659609/150‑1 (MQ=255)

                                                                                    aGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATTATCATCCACGACCAGAATCATCATATCGTCATTATAGCTGACCTATTTTTCCGTTGACGGCAGAGCGTTAGCAGGATAGTCGCTCTCCATCTCGATCaaat                                                                  >  1:713500/1‑150 (MQ=255)

                                                                                    aGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGTATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCaaat                                                                  >  2:4499994/1‑150 (MQ=255)

                                                                                    aGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCaaat                                                                  <  1:4850293/150‑1 (MQ=255)

                                                                                     gCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGTATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCaaata                                                                 >  2:3414102/1‑150 (MQ=255)

                                                                                       ccAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCaaataaa                                                               >  1:520113/1‑150 (MQ=255)

                                                                                        cAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGTATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCaaataaat                                                              <  2:1314565/150‑1 (MQ=255)

                                                                                         aaCGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCaaataa                                                                <  2:4989590/147‑1 (MQ=255)

                                                                                         aaCGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAaataaata                                                             >  2:2915028/1‑150 (MQ=255)

                                                                                           cGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATAcg                                                           <  2:2221688/150‑1 (MQ=255)

                                                                                             aTCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATAcgcg                                                         <  1:362492/150‑1 (MQ=255)

                                                                                             aTCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATAcgcg                                                         >  1:2305513/1‑150 (MQ=255)

                                                                                               cccAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGcc                                                       >  2:1781236/1‑150 (MQ=255)

                                                                                                  aaCTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGTATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCcaac                                                    >  1:1962/1‑150 (MQ=255)

                                                                                                   aCTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCcaaca                                                   >  1:2659350/1‑150 (MQ=255)

                                                                                                     tGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAAt                                                 <  1:5111428/150‑1 (MQ=255)

                                                                                                     tGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAAt                                                 <  2:4292039/150‑1 (MQ=255)

                                                                                                           gCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGTATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGt                                           >  1:3858422/1‑150 (MQ=255)

                                                                                                           gCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGt                                           >  1:3139876/1‑150 (MQ=255)

                                                                                                               gCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCt                                       <  2:1636709/150‑1 (MQ=255)

                                                                                                                cAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTc                                      <  1:4068187/150‑1 (MQ=255)

                                                                                                                 aaaCGCCGGTTAATCGGATGATCATCCACGACCAGTATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCa                                     >  2:5316051/1‑150 (MQ=255)

                                                                                                                 aaaCGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCa                                     >  1:1471650/1‑150 (MQ=255)

                                                                                                                    cGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGc                                  <  2:4534657/150‑1 (MQ=255)

                                                                                                                     gCCGGTTAATCGGATGATCATCCACGACCAGTATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCg                                 <  1:2114960/150‑1 (MQ=255)

                                                                                                                     gCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGTCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATgag                                 >  2:572669/1‑148 (MQ=255)

                                                                                                                           tAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGagcag                           >  2:3089861/1‑150 (MQ=255)

                                                                                                                            aaTCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGagcagc                          <  2:2793718/150‑1 (MQ=255)

                                                                                                                            aaTCGGATGATCATCCACGACCAGAATAATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGagcagc                          >  1:1794629/1‑150 (MQ=255)

                                                                                                                               cGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCcac                       >  2:1570517/1‑150 (MQ=255)

                                                                                                                                  atgatCATCCACGACCAGTATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACt                    <  2:3608411/150‑1 (MQ=255)

                                                                                                                                  atgatCATCCACGACCAGTATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACt                    <  1:1130964/150‑1 (MQ=255)

                                                                                                                                    gatCATCCACGACCAGTATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACtgt                  >  1:479560/1‑150 (MQ=255)

                                                                                                                                      tcatcCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTgtgt                <  2:5113352/150‑1 (MQ=255)

                                                                                                                                      tcatcCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTgtgt                >  1:3649711/1‑150 (MQ=255)

                                                                                                                                        atcCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTAc              >  1:2368568/1‑150 (MQ=255)

                                                                                                                                         tcCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTAcc             >  1:3601022/1‑150 (MQ=255)

                                                                                                                                          ccACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTAccc            <  2:1925237/150‑1 (MQ=255)

                                                                                                                                           cACGACCAGTATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCa           <  2:3987035/150‑1 (MQ=255)

                                                                                                                                           cACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCa           >  1:4355218/1‑150 (MQ=255)

                                                                                                                                                   gAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACTCCCCTg   <  2:2978921/150‑1 (MQ=255)

                                                                                                                                                    aaTCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACTCCCCTgg  >  1:2522329/1‑150 (MQ=255)

TGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACTCCCCTGG  >  CP009273/2310727‑2311024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A