Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,433,098 G→T 23.5% F87L (TTC→TTA)  rplQ ← 50S ribosomal subunit protein L17

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,433,0980GT23.5% 649.7 / 141.7 273F87L (TTC→TTA) rplQ50S ribosomal subunit protein L17
Reads supporting (aligned to +/- strand):  ref base G (112/96);  new base T (36/29);  total (148/125)
Fisher's exact test for biased strand distribution p-value = 8.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 284 total aligned reads displayed.

GGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCA  >  CP009273/3432949‑3433246
                                                                                                                                                     |                                                                                                                                                    
   gggTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGaaa                                                                                                                                                   >  1:2496839/1‑150 (MQ=255)
    ggTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAAc                                                                                                                                                  >  1:4270625/1‑150 (MQ=255)
       ttttttACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAAcgcg                                                                                                                                               >  2:378620/1‑150 (MQ=255)
         ttttACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCggg                                                                                                                                             <  2:2888104/150‑1 (MQ=255)
             aCGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCa                                                                                                                                         <  2:3386907/150‑1 (MQ=255)
             aCGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCa                                                                                                                                         <  1:1193458/150‑1 (MQ=255)
              cggtGCTTCAGATTACTCTGCAGCAGCTTCTACTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAg                                                                                                                                        <  2:126868/147‑1 (MQ=255)
               gTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGt                                                                                                                                       >  2:3901869/1‑150 (MQ=255)
                  gCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCTAAACGCGGGCCCAGTTCg                                                                                                                                    <  2:4504984/150‑1 (MQ=255)
                  gCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCTAAACGCGGGCCCAGTTCg                                                                                                                                    <  2:3637119/150‑1 (MQ=255)
                   cTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCTAAACGCGGGCCCAGTTCGt                                                                                                                                   >  1:359674/1‑150 (MQ=255)
                   cTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGt                                                                                                                                   >  2:2577084/1‑150 (MQ=255)
                     tCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTa                                                                                                                                 >  2:2717990/1‑150 (MQ=255)
                       aGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTaaa                                                                                                                               >  1:1441259/1‑150 (MQ=255)
                       aGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTaaa                                                                                                                               >  1:933666/1‑150 (MQ=255)
                          ttACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCTAAACGCGGGCCCAGTTCGTTAAACAg                                                                                                                            <  2:3798934/150‑1 (MQ=255)
                          ttACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAg                                                                                                                            <  2:1004681/150‑1 (MQ=255)
                              tctGCAGCAGCTTCTGCTTTCTCTGAACGATCACCCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGtttt                                                                                                                        <  1:5228533/150‑1 (MQ=255)
                              tctGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGtttt                                                                                                                        <  1:3274173/150‑1 (MQ=255)
                                  cagcagCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCa                                                                                                                    <  1:903335/150‑1 (MQ=255)
                                   agcagcTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCAc                                                                                                                   >  1:2260389/1‑150 (MQ=255)
                                   agcagcTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCAc                                                                                                                   <  1:1712655/150‑1 (MQ=255)
                                      agcTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGAt                                                                                                                >  1:1094467/1‑150 (MQ=255)
                                         ttCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGAtctc                                                                                                             <  2:1578575/150‑1 (MQ=255)
                                         ttCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGAtctc                                                                                                             <  2:4606226/150‑1 (MQ=255)
                                            tGCTTTCTCTGAACGATCAACCAGCTCGTTGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCggt                                                                                                          >  2:1336907/1‑148 (MQ=255)
                                            tGCTTTCTCTGAACGATCAACCAGCTCGATGTAATCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAAAACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGTCCAGTTCGTTAAACAGTTTTGCCACGATCTCGtt                                                                                                          >  2:3420038/1‑150 (MQ=255)
                                            tGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCTAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGtt                                                                                                          <  1:2449289/150‑1 (MQ=255)
                                              cTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTACCCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTAt                                                                                                        >  2:1603965/1‑150 (MQ=255)
                                                       aaCGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTAc                                                                                               >  2:3109149/1‑150 (MQ=255)
                                                       aaCGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTAc                                                                                               <  1:1020535/150‑1 (MQ=255)
                                                       aaCGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTAc                                                                                               <  1:1560702/150‑1 (MQ=255)
                                                       aaCGATCAACCAGCTCGAGGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTAc                                                                                               >  2:782720/1‑150 (MQ=255)
                                                         cGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACgg                                                                                             <  1:377710/150‑1 (MQ=255)
                                                          gATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACggg                                                                                            >  2:939183/1‑150 (MQ=255)
                                                           aTCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCTAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGc                                                                                           >  1:4637303/1‑150 (MQ=255)
                                                             cAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGa                                                                                         >  2:1350635/1‑150 (MQ=255)
                                                             cAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGa                                                                                         >  1:2152046/1‑150 (MQ=255)
                                                              aaCCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGTCACGATCTCGTTATCACGAGTACGGGCGaa                                                                                        >  2:2182964/1‑150 (MQ=255)
                                                              aaCCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGTCACGATCTCGTTATCACGAGTACGGGCGaa                                                                                        >  2:4991325/1‑150 (MQ=255)
                                                                  aGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGcc                                                                                    >  2:2615438/1‑150 (MQ=255)
                                                                  aGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGcc                                                                                    >  2:4016986/1‑150 (MQ=255)
                                                                     tCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAga                                                                                 <  1:4403150/150‑1 (MQ=255)
                                                                      cGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCTAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAgac                                                                                >  1:1475241/1‑150 (MQ=255)
                                                                            aaGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGAt                                                                          <  2:2399565/150‑1 (MQ=255)
                                                                             aGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGAtt                                                                         <  2:4092448/150‑1 (MQ=255)
                                                                                   cGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAAc                                                                   <  2:3272838/150‑1 (MQ=255)
                                                                                      cgcgTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCt                                                                <  2:315064/150‑1 (MQ=255)
                                                                                            gTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGt                                                          <  2:712565/150‑1 (MQ=255)
                                                                                             tCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTc                                                         >  2:651333/1‑150 (MQ=255)
                                                                                              cGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCTAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCt                                                        >  2:4034848/1‑150 (MQ=255)
                                                                                              cGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCt                                                        >  2:4271471/1‑150 (MQ=255)
                                                                                              cGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCt                                                        >  1:4858646/1‑150 (MQ=255)
                                                                                                ccTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTg                                                      <  2:2051588/150‑1 (MQ=255)
                                                                                                    cACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCaa                                                  >  1:23160/1‑150 (MQ=255)
                                                                                                       ggAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAagag                                               >  1:4122696/1‑150 (MQ=255)
                                                                                                       ggAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAagag                                               >  1:105683/1‑150 (MQ=255)
                                                                                                       ggAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAagag                                               >  2:1814687/1‑150 (MQ=255)
                                                                                                          aGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCTAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTaa                                            <  1:1964265/150‑1 (MQ=255)
                                                                                                          aGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTaa                                            >  2:123525/1‑150 (MQ=255)
                                                                                                           gCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAAt                                           <  1:3485822/150‑1 (MQ=255)
                                                                                                           gCCACACTTCAGAATACGAGTGGAACCACCGGCACGGCTCGCGACACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAAt                                           <  2:1937379/150‑1 (MQ=255)
                                                                                                            ccACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCTAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATc                                          >  1:63507/1‑150 (MQ=255)
                                                                                                            ccACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAATAGTAATc                                          <  1:773155/150‑1 (MQ=255)
                                                                                                             cacaCTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCa                                         >  1:4418249/1‑150 (MQ=255)
                                                                                                             cacaCTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCa                                         >  1:3961424/1‑150 (MQ=255)
                                                                                                               cacTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGc                                       <  2:2508115/150‑1 (MQ=255)
                                                                                                                acTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCTAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCg                                      <  2:5194147/150‑1 (MQ=255)
                                                                                                                acTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCTAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCg                                      <  1:3000274/150‑1 (MQ=255)
                                                                                                                acTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTATTCAGCg                                      <  1:5105574/150‑1 (MQ=255)
                                                                                                                acTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCg                                      <  2:1643867/150‑1 (MQ=255)
                                                                                                                 cTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCTAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCgg                                     >  1:2892266/1‑150 (MQ=255)
                                                                                                                 cTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCgg                                     <  2:2986092/150‑1 (MQ=255)
                                                                                                                   tCAGAATACGAGTGTAACCACCGGCACGGCTCGCTAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCt                                   <  2:4874649/150‑1 (MQ=255)
                                                                                                                   tCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCt                                   <  2:1141824/150‑1 (MQ=255)
                                                                                                                    cAGAATACGAGTGTAACCACCGGCACGGCTCGCTAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTc                                  <  2:176662/150‑1 (MQ=255)
                                                                                                                     aGAATACGAGTGTAACCACCGGCACGGCTCGCTAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCa                                 <  1:5093665/150‑1 (MQ=255)
                                                                                                                     aGAATACGAGTGTAACCACCGGCACGGCTCGCGACACGCGGGCCCAGTTCGTTAAACAGTTGTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCa                                 <  2:4413827/150‑1 (MQ=255)
                                                                                                                     aGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCa                                 >  2:538576/1‑150 (MQ=255)
                                                                                                                     aGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCa                                 >  1:2522112/1‑150 (MQ=255)
                                                                                                                       aaTACGAGTGTAACCACCGGCACGGCTCGCTAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCTACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAAc                               >  2:1710313/1‑150 (MQ=255)
                                                                                                                       aaTACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAAc                               >  1:4341677/1‑150 (MQ=255)
                                                                                                                          aCGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTAc                            <  1:3794505/150‑1 (MQ=255)
                                                                                                                           cGAGTGTAACCACCGGCACGGCTCGCTAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTAcg                           <  2:4867926/150‑1 (MQ=255)
                                                                                                                           cGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTAcg                           <  2:3739117/150‑1 (MQ=255)
                                                                                                                             aGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTAcgcg                         >  1:2390727/1‑150 (MQ=255)
                                                                                                                             aGTGTAACCACCGGCACGGCTCACTAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTAcgcg                         >  1:4985791/1‑150 (MQ=255)
                                                                                                                               tgtAACCACCGGCACGGCTCGCTAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTACgcggc                       >  1:1388979/1‑150 (MQ=255)
                                                                                                                               tgtAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTACgcggc                       <  2:2673632/150‑1 (MQ=255)
                                                                                                                                  aaCCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTACGCGGCGCa                    >  2:2208570/1‑150 (MQ=255)
                                                                                                                                     caccGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGct                 <  1:826263/150‑1 (MQ=255)
                                                                                                                                        cGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCtt              <  1:3067151/150‑1 (MQ=255)
                                                                                                                                         ggCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCttt             <  2:2755450/150‑1 (MQ=255)
                                                                                                                                          gCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCTTTATCACGATTACGGGCGAATGCCAGACGACTATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTc            <  1:1257692/150‑1 (MQ=255)
                                                                                                                                          gCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTc            <  2:4551758/150‑1 (MQ=255)
                                                                                                                                          gCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTc            <  2:5298257/150‑1 (MQ=255)
                                                                                                                                            aCGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTGTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACTATTAGCAACGCTATCAGTCTTGGCAAGATTATTCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGc          <  1:2717990/150‑1 (MQ=255)
                                                                                                                                               gCTCGCGAAACGCGGGCCCCGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCttt       <  1:4875881/150‑1 (MQ=255)
                                                                                                                                               gCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCttt       <  2:302254/150‑1 (MQ=255)
                                                                                                                                                   gcgAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGc   <  2:649727/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCCAGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCA  >  CP009273/3432949‑3433246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A