Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,729,221 C→A 25.9% R295S (CGT→AGT)  xylR → xylose divergent operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,729,2210CA25.9% 640.4 / 182.4 297R295S (CGT→AGT) xylRxylose divergent operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (113/107);  new base A (40/37);  total (153/144)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.22e-01

Only 100 of 302 total aligned reads displayed.

AGGCGGCAAAACTGTTGCATCGATTATTAGATAAAGAAGAAATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGT  >  CP009273/3729072‑3729370
                                                                                                                                                     |                                                                                                                                                     
aGGCGGCAAAACTGTTGCATCGATTATTAGATAAAGAAGAAATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTc                                                                                                                                                       <  1:4555597/150‑1 (MQ=255)
  gcggcAAAACTGTTGCATCGATTATTAGATAAAGAAGAAATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGt                                                                                                                                                     >  2:5110444/1‑150 (MQ=255)
  gcggcAAAACTGTTGCATCGATTATTAGATAAAGAAGAAATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTAGt                                                                                                                                                     <  1:4710163/150‑1 (MQ=255)
    ggcAAAACTGTTGCATCGATTATTAGATAAAGAAGAAATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTAGTaa                                                                                                                                                   >  2:3599653/1‑150 (MQ=255)
     gcAAAACTGTTGCATCGATTATTAGATAAAGAAGAAATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAAt                                                                                                                                                  <  1:962359/150‑1 (MQ=255)
     gcAAAACTGTTGCATCGATTATTAGATAAAGAAGAAATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTAGTAAt                                                                                                                                                  >  1:5223323/1‑150 (MQ=255)
      cAAAACTGTTGCATCGATTATTAGATAAAGAAGAAATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATc                                                                                                                                                 >  1:4305174/1‑150 (MQ=255)
       aaaaCTGTTTCTTCGATTATTAGATATAGATGAAATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGTCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCa                                                                                                                                                <  2:678883/150‑1 (MQ=255)
       aaaaCTGTTGCATCGATTATTAGATAAAGAAGAAATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTAGTAATCa                                                                                                                                                <  1:2023563/150‑1 (MQ=255)
        aaaCTGTTGCATCGATTATTAGATAAAGAAGAAATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCAc                                                                                                                                               >  1:1904481/1‑150 (MQ=255)
        aaaCTGTTGCATCGATTATTAGATAAAGAAGAAATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCAc                                                                                                                                               >  1:1072900/1‑150 (MQ=255)
        aaaCTGTTGCATCGATTATTAGATAAAGAAGAAATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTAGTAATCAc                                                                                                                                               <  1:438268/150‑1 (MQ=255)
         aaCTGTTGCATCGATTATTAGATAAAGAAGAAATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACg                                                                                                                                              >  2:2639910/1‑150 (MQ=255)
           cTGTTGCATCGATTATTAGATAAAGAAGAAATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGcc                                                                                                                                            >  2:725939/1‑150 (MQ=255)
             gTTGCATCGATTATTAGATAAAGAAGAAATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTg                                                                                                                                          >  1:1818979/1‑150 (MQ=255)
              ttGCATCGATTATTAGATAAAGAAGAAATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGt                                                                                                                                         <  2:4531672/150‑1 (MQ=255)
                 cATCGATTATTAGATAAAGAAGAAATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTAGTAATCACGCCTGTaaa                                                                                                                                      >  1:4555112/1‑150 (MQ=255)
                  aTCGATTATTAGATAAAGAAGAAATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAg                                                                                                                                     <  1:1614887/150‑1 (MQ=255)
                     gATTATTAGATAAAGAAGAAATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGa                                                                                                                                  <  1:2601664/150‑1 (MQ=255)
                     gATTATTAGATAAAGAAGAAATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGa                                                                                                                                  <  2:3940793/150‑1 (MQ=255)
                      attattAGATAAAGAAGAAATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGAt                                                                                                                                 >  2:4951606/1‑150 (MQ=255)
                       ttattaGATAAAGAAGAAATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGAtt                                                                                                                                <  1:1866942/150‑1 (MQ=255)
                       ttattaGATAAAGAAGAAATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGAtt                                                                                                                                >  1:425290/1‑150 (MQ=255)
                           aaGATAAAGAAGAAATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAg                                                                                                                            >  2:4846663/2‑150 (MQ=255)
                            aGATAAAGAAGAAATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGt                                                                                                                           <  1:2283568/150‑1 (MQ=255)
                            aGATAAAGAAGAAATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGt                                                                                                                           <  2:3493708/150‑1 (MQ=255)
                                 aagaagAAATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTAGTAATCACGCCTGTAAAGGGATTAAAGTGGATc                                                                                                                      >  2:1957306/1‑150 (MQ=255)
                                  agaagaAATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCa                                                                                                                     >  1:5125663/1‑150 (MQ=255)
                                  agaagaAATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCa                                                                                                                     >  1:3657118/1‑150 (MQ=255)
                                  agaagaAATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCa                                                                                                                     >  2:4186945/1‑150 (MQ=255)
                                   gaagaaATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAg                                                                                                                    >  2:1038285/1‑150 (MQ=255)
                                     agaaATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGt                                                                                                                  >  1:2974024/1‑150 (MQ=255)
                                      gaaATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTAGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTa                                                                                                                 >  1:5227433/1‑150 (MQ=255)
                                       aaaTGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTAc                                                                                                                >  2:4259345/1‑150 (MQ=255)
                                        aaTGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACt                                                                                                               <  2:4339123/150‑1 (MQ=255)
                                         aTGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTg                                                                                                              <  2:3139101/150‑1 (MQ=255)
                                               cTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCg                                                                                                        >  2:3777452/1‑150 (MQ=255)
                                                  cAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTc                                                                                                     >  2:2054782/1‑150 (MQ=255)
                                                  cAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTAGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTc                                                                                                     <  1:2465406/150‑1 (MQ=255)
                                                      gAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGa                                                                                                 <  1:5010316/150‑1 (MQ=255)
                                                       aaTTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGAt                                                                                                >  1:358502/1‑150 (MQ=255)
                                                          tttGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTAGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGAtctc                                                                                             <  2:3292075/150‑1 (MQ=255)
                                                          tttGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTAGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGAtctc                                                                                             >  1:4599258/1‑150 (MQ=255)
                                                            tGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTAGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTcgc                                                                                           >  2:1825100/1‑150 (MQ=255)
                                                                  caccaGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCa                                                                                     >  2:4167557/1‑150 (MQ=255)
                                                                   accaGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCaa                                                                                    >  2:1646942/1‑150 (MQ=255)
                                                                   accaGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCaa                                                                                    >  2:85144/1‑150 (MQ=255)
                                                                       gTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCtt                                                                                <  2:5232433/150‑1 (MQ=255)
                                                                        ttCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTg                                                                               <  1:120700/150‑1 (MQ=255)
                                                                         tCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTga                                                                              <  1:3134410/150‑1 (MQ=255)
                                                                          cgcgTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTgag                                                                             <  2:547969/150‑1 (MQ=255)
                                                                          cgcgTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTgag                                                                             <  2:3225584/150‑1 (MQ=255)
                                                                             gTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTAGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAg                                                                          >  2:227457/1‑150 (MQ=255)
                                                                                 ttGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGtt                                                                      >  2:1316057/1‑150 (MQ=255)
                                                                                  tGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGttt                                                                     <  1:102501/150‑1 (MQ=255)
                                                                                  tGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGttt                                                                     <  1:2390336/150‑1 (MQ=255)
                                                                                   gAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGtttt                                                                    >  1:2630569/1‑150 (MQ=255)
                                                                                    aaCGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTa                                                                   >  1:1129591/1‑150 (MQ=255)
                                                                                       ggCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAaag                                                                <  1:4932057/150‑1 (MQ=255)
                                                                                         cgcTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAaagaa                                                              <  1:4114396/150‑1 (MQ=255)
                                                                                            tCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAagag                                                           >  2:1123524/1‑150 (MQ=255)
                                                                                            tCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAagag                                                           <  1:931211/150‑1 (MQ=255)
                                                                                            tCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTAGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAagag                                                           <  2:60570/150‑1 (MQ=255)
                                                                                               aCAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAggtg                                                        <  2:2975698/150‑1 (MQ=255)
                                                                                                cAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTAGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAggtgg                                                       >  1:2774839/1‑150 (MQ=255)
                                                                                                  gATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTAGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAggtgggt                                                     <  2:4075179/150‑1 (MQ=255)
                                                                                                   aTTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAggtgggtg                                                    >  2:2921076/1‑150 (MQ=255)
                                                                                                       tcgctcgcTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAAc                                                <  2:2838003/150‑1 (MQ=255)
                                                                                                        cgctcgctGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAAcc                                               <  1:1397847/150‑1 (MQ=255)
                                                                                                        cgctcgctGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAAcc                                               <  2:3426489/150‑1 (MQ=255)
                                                                                                         gctcgctGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTAGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAAcaa                                              <  1:678258/150‑3 (MQ=255)
                                                                                                            cgctGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAAccatcc                                           <  1:36035/150‑1 (MQ=255)
                                                                                                             gctGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTAGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAAcaatcca                                          <  2:5292238/150‑1 (MQ=255)
                                                                                                               tGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATccatg                                        >  1:542802/1‑150 (MQ=255)
                                                                                                               tGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTAGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACAATccatg                                        >  1:1651157/1‑150 (MQ=255)
                                                                                                                gACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATccatgc                                       >  1:2832380/1‑150 (MQ=255)
                                                                                                                gACCGATCCCGCCGTTATTCAGGCCATGCATTACATTAGTAATCACGCCTGTAAAGTGATTAAAGTGGATCAGGTACTGGATGCGGTGGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACAATccatgc                                       <  1:1521894/150‑1 (MQ=255)
                                                                                                                gACCGATCCCGCCGTTATTCAGGCCATGCATTACATTAGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACAATccatgc                                       <  2:3625839/150‑1 (MQ=255)
                                                                                                                 aCCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATccatgcc                                      <  2:1071444/150‑1 (MQ=255)
                                                                                                                 aCCGATCCCGCCGTTATTCAGGCCATGCATTACATTAGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATccatgcc                                      <  2:1505876/150‑1 (MQ=255)
                                                                                                                  ccGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATccatgcca                                     <  2:3245270/150‑1 (MQ=255)
                                                                                                                    gATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATccatgccatg                                   >  2:2352058/1‑150 (MQ=255)
                                                                                                                    gATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATccatgccatg                                   >  2:1390837/1‑150 (MQ=255)
                                                                                                                     aTCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGa                                  >  1:321733/1‑150 (MQ=255)
                                                                                                                          gccgTTATTCAGGACATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCAt                             <  1:4125040/150‑1 (MQ=255)
                                                                                                                           ccgTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGAGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATg                            <  1:4050273/150‑1 (MQ=255)
                                                                                                                            cgTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGc                           <  2:3762215/150‑1 (MQ=255)
                                                                                                                            cgTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGc                           >  1:4797229/1‑150 (MQ=255)
                                                                                                                               tATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCga                        >  1:2778276/1‑150 (MQ=255)
                                                                                                                                  tCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGaa                     >  1:4444943/1‑150 (MQ=255)
                                                                                                                                    aGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGc                   <  1:5266999/150‑1 (MQ=255)
                                                                                                                                    aGGCCATGCATTACATTAGTTATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTTCTGGATGCGGTCGGTATCTCGCGCTCCAATCTTGATAAGCGTTTTAAAGAAGAGGTGGGTGAAACAATCCATGCCATGATTCATGCCGAGAAGc                   <  1:3357046/150‑1 (MQ=255)
                                                                                                                                     ggCCATGCATTACATTAGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCt                  <  2:4795755/150‑1 (MQ=255)
                                                                                                                                      gCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTg                 <  1:85144/150‑1 (MQ=255)
                                                                                                                                      gCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTg                 <  1:76087/150‑1 (MQ=255)
                                                                                                                                             aTTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAg          <  2:251387/150‑1 (MQ=255)
                                                                                                                                             aTTACATTAGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACAATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAg          >  2:795570/1‑150 (MQ=255)
                                                                                                                                              ttACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCTATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAgc         <  1:4871538/150‑1 (MQ=255)
                                                                                                                                              ttACATTAGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACAATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAgc         <  2:108089/150‑1 (MQ=255)
                                                                                                                                               tACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAgcg        <  2:334558/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGGCGGCAAAACTGTTGCATCGATTATTAGATAAAGAAGAAATGCCGCTACAGCGAATTTTGGTCCCACCAGTTCGCGTCATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGT  >  CP009273/3729072‑3729370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A