Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F527 I0 R1	1651	322.4	10661268	95.2%	10149527	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,154,402	A→T	68.6%	E279V (GAA→GTA)	btuB →	vitamin B12/cobalamin outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,154,402	0	A	T	68.6%	409.2 / 269.3	289	E279V (GAA→GTA)	btuB	vitamin B12/cobalamin outer membrane transporter
Reads supporting (aligned to +/- strand): ref base A (37/54); new base T (87/111); total (124/165)
Fisher's exact test for biased strand distribution p-value = 6.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 291 total aligned reads displayed.

CTTTACTGATGCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACG  >  CP009273/4154253‑4154551

   tACTGATGCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACt                                                                                                                                                    <  2:2961462/150‑1 (MQ=255)

   tACTGATGCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACt                                                                                                                                                    <  2:1850337/150‑1 (MQ=255)

            cTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATc                                                                                                                                           <  2:3602464/150‑1 (MQ=255)

            cTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGACCTGATTAAATc                                                                                                                                           <  1:2768806/150‑1 (MQ=255)

              ggAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATcac                                                                                                                                         <  2:4282127/150‑1 (MQ=255)

                aGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACaa                                                                                                                                       >  1:207166/1‑150 (MQ=255)

                  cGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACt                                                                                                                                     <  1:750670/150‑1 (MQ=255)

                     cgttGTGCGCGGCTATGGCTATGATAACCGTACCACTTATGACGCGTATTATTCTCCCGGTTCACCGTTGCCCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCAt                                                                                                                                  <  2:3824862/148‑1 (MQ=255)

                     cTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCAt                                                                                                                                  <  1:1266169/150‑1 (MQ=255)

                      tttGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCAtt                                                                                                                                 <  2:4743379/150‑1 (MQ=255)

                      tttGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCAtt                                                                                                                                 <  2:25417/150‑1 (MQ=255)

                        tgtgCGCGGCTATGGCTATGATAACCTTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTAc                                                                                                                               <  2:4899983/150‑1 (MQ=255)

                          tgCGCGGCTATGGCTATGATAACCGTACCTATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCa                                                                                                                             <  1:2587188/150‑1 (MQ=255)

                          tgCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAATTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCa                                                                                                                             >  2:4370239/1‑150 (MQ=255)

                              cgGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCta                                                                                                                         >  2:3463491/1‑150 (MQ=255)

                                 ctatggctatgATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAg                                                                                                                      <  2:2329301/150‑1 (MQ=255)

                                     ggctatgTTAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCAt                                                                                                                  >  2:2864602/1‑150 (MQ=255)

                                     ggctatgATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCAt                                                                                                                  <  2:1995458/150‑1 (MQ=255)

                                     ggctatgATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCAt                                                                                                                  <  2:1575847/150‑1 (MQ=255)

                                      gctatgATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATa                                                                                                                 <  2:4470015/150‑1 (MQ=255)

                                        tatgATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGc                                                                                                               <  1:418975/150‑1 (MQ=255)

                                        tatgATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGc                                                                                                               <  1:4250313/150‑1 (MQ=255)

                                          tgATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCaa                                                                                                             >  1:376081/1‑150 (MQ=255)

                                              aaCCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGAt                                                                                                         <  2:3421596/150‑1 (MQ=255)

                                              aaCCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGAt                                                                                                         <  1:1573039/150‑1 (MQ=255)

                                                 cGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTAc                                                                                                      >  1:2720994/1‑150 (MQ=255)

                                                 cGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTAc                                                                                                      >  2:3225/1‑150 (MQ=255)

                                                   tACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACaa                                                                                                    >  2:2395152/1‑150 (MQ=255)

                                                   tACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACaa                                                                                                    >  2:1390873/1‑150 (MQ=255)

                                                     ccAATTATGACGCGTATTATTCTCCCGGTTCANCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACt                                                                                                  <  1:1192994/150‑1 (MQ=255)

                                                     ccAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACt                                                                                                  <  2:5324409/150‑1 (MQ=255)

                                                        aTTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACg                                                                                               >  1:3605129/1‑150 (MQ=255)

                                                        aTTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACg                                                                                               >  2:1772847/1‑150 (MQ=255)

                                                        aTTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTCCACCTCCg                                                                                               >  2:4582735/1‑150 (MQ=255)

                                                         ttATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGa                                                                                              <  1:4051183/150‑1 (MQ=255)

                                                         ttATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGa                                                                                              <  1:578423/150‑1 (MQ=255)

                                                           aTGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATc                                                                                            >  2:3186420/1‑150 (MQ=255)

                                                            tGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATcc                                                                                           >  1:4708054/1‑150 (MQ=255)

                                                            tGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTAGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATcc                                                                                           >  2:5103258/1‑150 (MQ=255)

                                                             gaagcgTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTCATTAAATCACAACTCATTACCAGCTATAGCCCTAGCAAAGAATACAACTACGATccc                                                                                          >  2:5003367/4‑150 (MQ=255)

                                                              aCGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATcccc                                                                                         <  1:2719679/150‑1 (MQ=255)

                                                               cgcgTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCa                                                                                        <  1:2371673/150‑1 (MQ=255)

                                                                gcgTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCAt                                                                                       >  1:5272636/1‑150 (MQ=255)

                                                                 cgTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCAtt                                                                                      <  2:4155444/150‑1 (MQ=255)

                                                                  gTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTa                                                                                     <  1:3708/150‑1 (MQ=255)

                                                                   tattatTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTAt                                                                                    >  1:986944/1‑150 (MQ=255)

                                                                    attattCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATg                                                                                   <  2:3659280/150‑1 (MQ=255)

                                                                       attCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTc                                                                                <  2:3142491/150‑1 (MQ=255)

                                                                        ttCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCg                                                                               <  1:4383772/150‑1 (MQ=255)

                                                                            cccGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTAt                                                                           <  1:2256719/150‑1 (MQ=255)

                                                                            cccGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTAt                                                                           >  2:1157577/1‑150 (MQ=255)

                                                                                 ttCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATtc                                                                      <  1:5210112/150‑1 (MQ=255)

                                                                                  tCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATtcg                                                                     >  2:2050408/1‑150 (MQ=255)

                                                                                   cACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATtcgt                                                                    >  1:1513770/1‑150 (MQ=255)

                                                                                   cACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCAGTATGGTCGTTATGATtcgt                                                                    >  2:392222/1‑150 (MQ=255)

                                                                                   cACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATtcgt                                                                    >  1:3787784/1‑150 (MQ=255)

                                                                                    aCCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATtcgtc                                                                   >  1:3285829/1‑150 (MQ=255)

                                                                                        ttGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCg                                                               >  1:4199344/1‑150 (MQ=255)

                                                                                        ttGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCg                                                               <  1:3381284/150‑1 (MQ=255)

                                                                                        ttGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCg                                                               <  2:4407995/150‑1 (MQ=255)

                                                                                         tGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGa                                                              <  1:656494/150‑1 (MQ=255)

                                                                                          gCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGAc                                                             <  1:886492/150‑1 (MQ=255)

                                                                                           cTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACg                                                            >  2:406115/1‑150 (MQ=255)

                                                                                              gATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTc                                                         >  2:4696818/1‑150 (MQ=255)

                                                                                                tACCCTTAAACTCTTTATCCACAGTTTGGACGCAGTGCTGCGCTATATAGGATTACTGATTTAATCACTACTCATTACCAGCTATAGCCTTAGCAAAGATTACAACTACGATCCCCTTTATTGTCTTTTTGATTCGTCGGCGAAGCTCga                                                       <  2:667945/150‑1 (MQ=255)

                                                                                                tACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCga                                                       >  1:3166912/1‑150 (MQ=255)

                                                                                                 aCCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCgat                                                      >  2:2821781/1‑150 (MQ=255)

                                                                                                  cccGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCgatg                                                     <  2:3379553/150‑1 (MQ=255)

                                                                                                  cccGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCgatg                                                     <  1:5269766/150‑1 (MQ=255)

                                                                                                   ccGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCgatga                                                    <  2:1550898/150‑1 (MQ=255)

                                                                                                    cGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCgatgag                                                   >  1:4036341/1‑150 (MQ=255)

                                                                                                       aaaCTCTATAGCCAAAGTTGGGACGCCGGGCTGCGATATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCgatgagatg                                                <  1:225710/150‑1 (MQ=255)

                                                                                                        aaCTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCgatgagatga                                               >  1:1791130/1‑150 (MQ=255)

                                                                                                        aaCTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCgatgagatga                                               >  2:2212762/1‑150 (MQ=255)

                                                                                                          ctctATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAg                                             <  2:2446764/150‑1 (MQ=255)

                                                                                                          ctctATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAg                                             >  1:4931572/1‑150 (MQ=255)

                                                                                                           tctATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGc                                            >  1:186306/1‑150 (MQ=255)

                                                                                                            ctATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGAGGAGATGAAGCa                                           >  1:4625039/1‑150 (MQ=255)

                                                                                                            ctATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCa                                           >  2:751741/1‑150 (MQ=255)

                                                                                                             tataGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCaa                                          >  2:353149/1‑150 (MQ=255)

                                                                                                               taGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATa                                        >  1:2939523/1‑150 (MQ=255)

                                                                                                                aGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATac                                       <  1:1990798/150‑1 (MQ=255)

                                                                                                                 gCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATaca                                      <  2:2150295/150‑1 (MQ=255)

                                                                                                                  ccAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATacac                                     <  1:1146976/150‑1 (MQ=255)

                                                                                                                  ccAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATacac                                     <  1:4410939/150‑1 (MQ=255)

                                                                                                                    aaaGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCg                                   <  2:1617422/150‑1 (MQ=255)

                                                                                                                         tGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAg                              <  1:5029708/150‑1 (MQ=255)

                                                                                                                           ggACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATGAGGGTCGTGATGATTAGTCGGCGCCGCGCCAAGTGATGAAGCAATACACCGTCCAgag                            >  1:4507828/1‑148 (MQ=255)

                                                                                                                            gACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAGTgg                           >  1:4269870/1‑150 (MQ=255)

                                                                                                                                ccGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAGTGGGCaa                       <  1:3639665/150‑1 (MQ=255)

                                                                                                                                   ggCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGAGGAGATGAAGCAATACACCGTCCAGTGGGCAAACa                    >  1:224320/1‑150 (MQ=255)

                                                                                                                                     cTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAGTGGGCAAACAAt                  >  2:2710747/1‑150 (MQ=255)

                                                                                                                                             taACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAGTGGGCAAACAATGTCATCGt          >  2:1038448/1‑150 (MQ=255)

                                                                                                                                             taACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAGTGGGCAAACAATGTCATCGt          >  2:5215029/1‑150 (MQ=255)

                                                                                                                                               aCGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAGTGGGCAAACAATGTCATCGTTg        <  1:3108028/150‑1 (MQ=255)

                                                                                                                                                cGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAGTGGGCAAACAATGTCATCGTTgg       <  1:2869689/150‑1 (MQ=255)

                                                                                                                                                cGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAGTGGGCAAACAATGTCATCGTTgg       <  2:1184529/150‑1 (MQ=255)

                                                                                                                                                  gCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTc     >  1:1741974/1‑150 (MQ=255)

                                                                                                                                                  gCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTc     <  2:3066439/150‑1 (MQ=255)

                                                                                                                                                   cGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCa    <  2:4608401/150‑1 (MQ=255)

CTTTACTGATGCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACG  >  CP009273/4154253‑4154551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTTACTGATGCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACG  >  CP009273/4154254‑4154551

TTTACTGATGCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTA                                                                                                                                                      >  SRR19973062.3757060/1‑150 (MQ=60)

 TTACTGATGCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAAC                                                                                                                                                     <  SRR19973062.2116868/150‑1 (MQ=60)

 TTACTGATGCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAAC                                                                                                                                                     <  SRR19973062.3675089/150‑1 (MQ=60)

  TACTGATGCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACT                                                                                                                                                    <  SRR19973062.5300161/150‑1 (MQ=60)

    CTGATGCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGA                                                                                                                                                  >  SRR19973062.4097244/1‑150 (MQ=60)

     TGATGCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGAT                                                                                                                                                 >  SRR19973062.630684/1‑150 (MQ=60)

     TGATGCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGAT                                                                                                                                                 <  SRR19973062.701696/150‑1 (MQ=60)

      GATGCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATT                                                                                                                                                <  SRR19973062.1615679/150‑1 (MQ=60)

      GATGCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATT                                                                                                                                                <  SRR19973062.3766743/150‑1 (MQ=60)

       ATGCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTA                                                                                                                                               <  SRR19973062.506941/150‑1 (MQ=60)

        TGCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAA                                                                                                                                              <  SRR19973062.3128042/150‑1 (MQ=60)

          CCTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAAT                                                                                                                                            >  SRR19973062.5246523/1‑150 (MQ=60)

           CTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGACCTGATTAAATC                                                                                                                                           <  SRR19973062.2783429/150‑1 (MQ=60)

            TGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCA                                                                                                                                          >  SRR19973062.1930543/1‑150 (MQ=60)

              GAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACA                                                                                                                                        <  SRR19973062.467446/150‑1 (MQ=60)

               AGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAA                                                                                                                                       >  SRR19973062.208266/1‑150 (MQ=60)

                GCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAAC                                                                                                                                      <  SRR19973062.1766184/150‑1 (MQ=60)

                 CGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACT                                                                                                                                     <  SRR19973062.754069/150‑1 (MQ=60)

                 CGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACT                                                                                                                                     <  SRR19973062.2267321/150‑1 (MQ=60)

                    CTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCAT                                                                                                                                  <  SRR19973062.3740239/150‑1 (MQ=60)

                    CTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCAT                                                                                                                                  <  SRR19973062.1272005/150‑1 (MQ=60)

                    CTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCAT                                                                                                                                  <  SRR19973062.2461940/150‑1 (MQ=60)

                     TTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTGAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATT                                                                                                                                 <  SRR19973062.4221526/150‑1 (MQ=60)

                     TTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATT                                                                                                                                 <  SRR19973062.1845828/150‑1 (MQ=60)

                     TTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATT                                                                                                                                 <  SRR19973062.26335/150‑1 (MQ=60)

                     TTTGTGCGCGGCTATGGCTATGATAACCGTAACAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATT                                                                                                                                 <  SRR19973062.3065324/150‑1 (MQ=60)

                       TGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTAC                                                                                                                               <  SRR19973062.4183997/150‑1 (MQ=60)

                        GTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACC                                                                                                                              >  SRR19973062.968998/1‑150 (MQ=60)

                         TGCGCGGCTATGGCTATGATAACCGTACCTATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCA                                                                                                                             <  SRR19973062.2600907/150‑1 (MQ=60)

                          GCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAG                                                                                                                            >  SRR19973062.5194505/1‑150 (MQ=60)

                            GCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCT                                                                                                                          <  SRR19973062.3231293/150‑1 (MQ=60)

                                CTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAG                                                                                                                      >  SRR19973062.4611786/1‑150 (MQ=60)

                                   TGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCA                                                                                                                   >  SRR19973062.3982977/1‑150 (MQ=60)

                                      CTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAG                                                                                                                <  SRR19973062.1803991/150‑1 (MQ=60)

                                       TATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGC                                                                                                               <  SRR19973062.420799/150‑1 (MQ=60)

                                       TATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGC                                                                                                               <  SRR19973062.420781/150‑1 (MQ=60)

                                       TATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGC                                                                                                               <  SRR19973062.4272325/150‑1 (MQ=60)

                                       TATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGC                                                                                                               <  SRR19973062.4272398/150‑1 (MQ=60)

                                         TGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAA                                                                                                             >  SRR19973062.377762/1‑150 (MQ=60)

                                          GATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAA                                                                                                            >  SRR19973062.2010608/1‑150 (MQ=60)

                                           ATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAG                                                                                                           >  SRR19973062.5045191/1‑150 (MQ=60)

                                             AACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGAT                                                                                                         <  SRR19973062.897657/150‑1 (MQ=60)

                                             AACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGAT                                                                                                         <  SRR19973062.364709/150‑1 (MQ=60)

                                             AACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGAT                                                                                                         <  SRR19973062.1580818/150‑1 (MQ=60)

                                             AACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGAT                                                                                                         >  SRR19973062.2295341/1‑150 (MQ=60)

                                              ACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATT                                                                                                        <  SRR19973062.5237974/150‑1 (MQ=60)

                                               CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTA                                                                                                       >  SRR19973062.1345120/1‑150 (MQ=60)

                                               CCGGACCAATTCTGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTA                                                                                                       <  SRR19973062.2785074/150‑1 (MQ=60)

                                                CGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTAC                                                                                                      >  SRR19973062.3260498/1‑150 (MQ=60)

                                                CGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTAC                                                                                                      >  SRR19973062.2735309/1‑150 (MQ=60)

                                                    CCAATTATGACGCGTATTATTCTCCCGGTTCANCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACT                                                                                                  <  SRR19973062.1198570/150‑1 (MQ=60)

                                                      AATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTAC                                                                                                >  SRR19973062.824956/1‑150 (MQ=60)

                                                      AATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTAC                                                                                                <  SRR19973062.2599539/150‑1 (MQ=60)

                                                       ATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACG                                                                                               >  SRR19973062.3623464/1‑150 (MQ=60)

                                                       ATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACG                                                                                               >  SRR19973062.3623704/1‑150 (MQ=60)

                                                        TTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGA                                                                                              <  SRR19973062.4071902/150‑1 (MQ=60)

                                                        TTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGA                                                                                              <  SRR19973062.581128/150‑1 (MQ=60)

                                                           TGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCC                                                                                           >  SRR19973062.4732633/1‑150 (MQ=60)

                                                             ACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCC                                                                                         <  SRR19973062.3824121/150‑1 (MQ=60)

                                                             ACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCC                                                                                         <  SRR19973062.2734201/150‑1 (MQ=60)

                                                             ACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCC                                                                                         <  SRR19973062.2733998/150‑1 (MQ=60)

                                                             ACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCC                                                                                         <  SRR19973062.2733989/150‑1 (MQ=60)

                                                              CGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCA                                                                                        <  SRR19973062.2384370/150‑1 (MQ=60)

                                                               GCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCAT                                                                                       >  SRR19973062.5300372/1‑150 (MQ=60)

                                                                CGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATT                                                                                      <  SRR19973062.4212852/150‑1 (MQ=60)

                                                                CGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATT                                                                                      <  SRR19973062.1916238/150‑1 (MQ=60)

                                                                CGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATT                                                                                      <  SRR19973062.3781512/150‑1 (MQ=60)

                                                                 GTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTA                                                                                     <  SRR19973062.3732/150‑1 (MQ=60)

                                                                  TATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTAT                                                                                    >  SRR19973062.991528/1‑150 (MQ=60)

                                                                   ATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATG                                                                                   >  SRR19973062.1258883/1‑150 (MQ=60)

                                                                    TTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAATATTACAACTACGATACCCATTATGG                                                                                  >  SRR19973062.2012395/1‑150 (MQ=60)

                                                                      ATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTC                                                                                >  SRR19973062.1510309/1‑150 (MQ=60)

                                                                      ATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTC                                                                                >  SRR19973062.4448847/1‑150 (MQ=60)

                                                                       TTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCG                                                                               <  SRR19973062.4406243/150‑1 (MQ=60)

                                                                         CTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCAGTATGGTCGTT                                                                             >  SRR19973062.3161074/1‑150 (MQ=60)

                                                                           CCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTAT                                                                           <  SRR19973062.2268029/150‑1 (MQ=60)

                                                                           CCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTAT                                                                           <  SRR19973062.2268161/150‑1 (MQ=60)

                                                                           CCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTAT                                                                           <  SRR19973062.2835757/150‑1 (MQ=60)

                                                                              GGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGAT                                                                        >  SRR19973062.5311960/1‑150 (MQ=60)

                                                                                TTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTC                                                                      <  SRR19973062.5237592/150‑1 (MQ=60)

                                                                                  CACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGT                                                                    >  SRR19973062.1521230/1‑150 (MQ=60)

                                                                                  CACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGT                                                                    >  SRR19973062.3807186/1‑150 (MQ=60)

                                                                                   ACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTC                                                                   >  SRR19973062.4429884/1‑150 (MQ=60)

                                                                                   ACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTC                                                                   >  SRR19973062.2763032/1‑150 (MQ=60)

                                                                                   ACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTC                                                                   >  SRR19973062.4429833/1‑150 (MQ=60)

                                                                                   ACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTC                                                                   >  SRR19973062.3302847/1‑150 (MQ=60)

                                                                                     CGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGG                                                                 <  SRR19973062.4673707/150‑1 (MQ=60)

                                                                                       TTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCG                                                               <  SRR19973062.2324832/150‑1 (MQ=60)

                                                                                       TTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCG                                                               <  SRR19973062.3398669/150‑1 (MQ=60)

                                                                                       TTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCG                                                               >  SRR19973062.4221131/1‑150 (MQ=60)

                                                                                        TGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGA                                                              <  SRR19973062.659556/150‑1 (MQ=60)

                                                                                         GCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGAC                                                             <  SRR19973062.890287/150‑1 (MQ=60)

                                                                                               TACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGA                                                       <  SRR19973062.3539474/150‑1 (MQ=60)

                                                                                               TACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGA                                                       >  SRR19973062.3183319/1‑150 (MQ=60)

                                                                                                 CCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATG                                                     <  SRR19973062.5297487/150‑1 (MQ=60)

                                                                                                   CGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAG                                                   >  SRR19973062.4057233/1‑150 (MQ=60)

                                                                                                   CGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAG                                                   >  SRR19973062.4057027/1‑150 (MQ=60)

                                                                                                   CGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAG                                                   >  SRR19973062.4056899/1‑150 (MQ=60)

                                                                                                   CGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAG                                                   <  SRR19973062.610896/150‑1 (MQ=60)

                                                                                                    GTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGA                                                  >  SRR19973062.2934583/1‑150 (MQ=60)

                                                                                                    GTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGA                                                  >  SRR19973062.2934649/1‑150 (MQ=60)

                                                                                                     TAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGAT                                                 <  SRR19973062.1522496/150‑1 (MQ=60)

                                                                                                      AAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATG                                                <  SRR19973062.226820/150‑1 (MQ=60)

                                                                                                      AAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATG                                                >  SRR19973062.378280/1‑150 (MQ=60)

                                                                                                      AAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCACGATGAGATG                                                >  SRR19973062.2204055/1‑150 (MQ=60)

                                                                                                      AAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGATATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATG                                                <  SRR19973062.226877/150‑1 (MQ=60)

                                                                                                       AACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAATGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGA                                               <  SRR19973062.4527611/150‑1 (MQ=60)

                                                                                                       AACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGA                                               >  SRR19973062.1799800/1‑150 (MQ=60)

                                                                                                       AACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGA                                               <  SRR19973062.1576593/150‑1 (MQ=60)

                                                                                                       AACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGA                                               <  SRR19973062.3714323/150‑1 (MQ=60)

                                                                                                         CTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAG                                             >  SRR19973062.4957323/1‑150 (MQ=60)

                                                                                                          TCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGC                                            >  SRR19973062.187321/1‑150 (MQ=60)

                                                                                                           CTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGAGGAGATGAAGCA                                           >  SRR19973062.4649235/1‑150 (MQ=60)

                                                                                                             ATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAAT                                         >  SRR19973062.4612565/1‑150 (MQ=60)

                                                                                                              TAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATA                                        >  SRR19973062.1586538/1‑150 (MQ=60)

                                                                                                              TAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATA                                        >  SRR19973062.2954987/1‑150 (MQ=60)

                                                                                                               AGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATAC                                       <  SRR19973062.2745107/150‑1 (MQ=60)

                                                                                                               AGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATAC                                       <  SRR19973062.4960687/150‑1 (MQ=60)

                                                                                                               AGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATAC                                       <  SRR19973062.2001043/150‑1 (MQ=60)

                                                                                                               AGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATAC                                       <  SRR19973062.3044552/150‑1 (MQ=60)

                                                                                                                 CCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACAC                                     <  SRR19973062.1152443/150‑1 (MQ=60)

                                                                                                                 CCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACAC                                     <  SRR19973062.1152350/150‑1 (MQ=60)

                                                                                                                 CCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACAC                                     <  SRR19973062.4433494/150‑1 (MQ=60)

                                                                                                                    AAGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGT                                  >  SRR19973062.674033/1‑150 (MQ=60)

                                                                                                                     AGTTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTC                                 >  SRR19973062.795537/1‑150 (MQ=60)

                                                                                                                       TTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCA                               >  SRR19973062.4870661/1‑150 (MQ=60)

                                                                                                                       TTGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCA                               <  SRR19973062.433593/150‑1 (MQ=60)

                                                                                                                        TGGGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAG                              <  SRR19973062.5056365/150‑1 (MQ=60)

                                                                                                                        TGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAG                              >  SRR19973062.1237060/1‑150 (MQ=60)

                                                                                                                          GGACGCCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATGAGGGTCGTGATGATTAGTCGGCGCCGCGCCAAGTGATGAAGCAATACACCGTCCAGAG                            >  SRR19973062.4531191/1‑150 (MQ=60)

                                                                                                                           GACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAGTGG                           >  SRR19973062.4291965/1‑150 (MQ=60)

                                                                                                                               CCGGGCTGCGCTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAGTGGGCAA                       <  SRR19973062.3658297/150‑1 (MQ=60)

                                                                                                                                  GGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGAGGAGATGAAGCAATACACCGTCCAGTGGGCAAACA                    >  SRR19973062.225485/1‑150 (MQ=60)

                                                                                                                                     TGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAGTGGGCAAACAATG                 <  SRR19973062.425652/150‑1 (MQ=60)

                                                                                                                                         CTATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAGTGGGCAAACAATGTCAT             >  SRR19973062.4739573/1‑150 (MQ=60)

                                                                                                                                          TATAACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAGTGGGCAAACAATGTCATC            <  SRR19973062.2688736/150‑1 (MQ=60)

                                                                                                                                          TATAACGGAGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAGTGGGCAAACAATGTCATC            <  SRR19973062.5299276/150‑1 (MQ=60)

                                                                                                                                              ACGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAGTGGGCAAACAATGTCATCGTTG        <  SRR19973062.3124031/150‑1 (MQ=60)

                                                                                                                                               CGGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGG       <  SRR19973062.2884868/150‑1 (MQ=60)

                                                                                                                                                GGCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGT      >  SRR19973062.341604/1‑150 (MQ=60)

                                                                                                                                                 GCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTC     >  SRR19973062.1750499/1‑150 (MQ=60)

                                                                                                                                                 GCGTACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTC     >  SRR19973062.1750448/1‑150 (MQ=60)

                                                                                                                                                  CGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCA    <  SRR19973062.4334153/150‑1 (MQ=60)

                                                                                                                                                    AACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACG  <  SRR19973062.3392813/150‑1 (MQ=60)

TTTACTGATGCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACG  >  CP009273/4154254‑4154551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`