Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F60 I0 R1 465 320.4 10502068 95.5% 10029474 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 147,946 G→T 47.6% I47I (ATC→ATA)  yadK ← putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273147,9460GT47.6% 137.3 / inf 252I47I (ATC→ATA) yadKputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base G (68/64);  new base T (47/73);  total (115/137)
Fisher's exact test for biased strand distribution p-value = 5.78e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 255 total aligned reads displayed.

TTTAGCACATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTT  >  CP009273/147800‑148094
                                                                                                                                                  |                                                                                                                                                    
tttAGCACATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTAt                                                                                                                                                    <  2:1766859/149‑1 (MQ=255)
tttAGCACATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGAt                                                                                                                                                    >  1:2177155/1‑149 (MQ=255)
    gCACATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCg                                                                                                                                                >  2:572646/1‑149 (MQ=255)
    gCACATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCg                                                                                                                                                >  2:4049209/1‑149 (MQ=255)
       caTCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCtt                                                                                                                                             >  1:5186890/1‑149 (MQ=255)
        aTCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCttt                                                                                                                                            >  1:2828761/1‑149 (MQ=255)
          cGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTg                                                                                                                                          <  2:738467/149‑1 (MQ=255)
           gCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGc                                                                                                                                         <  2:4298647/149‑1 (MQ=255)
           gCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGc                                                                                                                                         <  2:3466668/149‑1 (MQ=255)
            ccAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCa                                                                                                                                        >  1:1795745/1‑149 (MQ=255)
              aGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAgg                                                                                                                                      >  2:870234/1‑149 (MQ=255)
                ttCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTg                                                                                                                                    >  1:4782863/1‑149 (MQ=255)
                 tCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGc                                                                                                                                   <  1:5130411/149‑1 (MQ=255)
                  cccTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCt                                                                                                                                  <  2:3565990/149‑1 (MQ=255)
                  cccTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCt                                                                                                                                  <  2:1465621/149‑1 (MQ=255)
                   ccTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTg                                                                                                                                 >  2:2600195/1‑149 (MQ=255)
                   ccTGTAATGCACAGTCGGAGACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTg                                                                                                                                 <  2:3740284/149‑1 (MQ=255)
                    cTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGt                                                                                                                                <  2:3010466/149‑1 (MQ=255)
                    cTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGt                                                                                                                                <  2:2293547/149‑1 (MQ=255)
                    cTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGt                                                                                                                                <  2:4260017/149‑1 (MQ=255)
                    cTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGt                                                                                                                                <  2:1643258/149‑1 (MQ=255)
                       tAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGtttt                                                                                                                             <  2:3374443/149‑1 (MQ=255)
                             acaGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACAt                                                                                                                       >  2:3763736/1‑149 (MQ=255)
                              caGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACAtt                                                                                                                      <  1:960629/149‑1 (MQ=255)
                                   ggATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGcaa                                                                                                                 >  2:1164141/1‑149 (MQ=255)
                                   ggATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGcaa                                                                                                                 <  2:459009/149‑1 (MQ=255)
                                    gATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGcaac                                                                                                                >  1:3640017/1‑149 (MQ=255)
                                    gATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGcaac                                                                                                                >  1:424496/1‑149 (MQ=255)
                                     aTACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGcaaca                                                                                                               >  1:4011623/1‑149 (MQ=255)
                                          gTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTc                                                                                                          >  1:3442789/1‑149 (MQ=255)
                                           ttaTAGTGAACTCTTGTCCACCTGTTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCa                                                                                                         <  1:721565/147‑1 (MQ=255)
                                            gATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCaa                                                                                                        <  1:1486214/149‑1 (MQ=255)
                                             aTAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAat                                                                                                       >  1:2051352/1‑149 (MQ=255)
                                             aTAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAat                                                                                                       >  2:3672619/1‑149 (MQ=255)
                                             aTAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAat                                                                                                       <  1:1637557/149‑1 (MQ=255)
                                                gTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATc                                                                                                    >  2:2873706/1‑149 (MQ=255)
                                                  gAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCt                                                                                                  <  2:3285750/149‑1 (MQ=255)
                                                   aaCTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTg                                                                                                 >  2:2752534/1‑149 (MQ=255)
                                                   aaCTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTg                                                                                                 <  1:1820208/149‑1 (MQ=255)
                                                      tctTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTcc                                                                                              <  1:3532028/149‑1 (MQ=255)
                                                           tCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCaa                                                                                         <  2:4481263/149‑1 (MQ=255)
                                                            ccACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAAt                                                                                        <  1:2838285/149‑1 (MQ=255)
                                                                cTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCt                                                                                    <  1:3350515/149‑1 (MQ=255)
                                                                  ggTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTgg                                                                                  <  1:4605131/149‑1 (MQ=255)
                                                                    ttGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGAc                                                                                >  1:1722013/1‑149 (MQ=255)
                                                                    ttGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGAc                                                                                >  2:2557036/1‑149 (MQ=255)
                                                                        tAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGc                                                                            >  1:3895141/1‑149 (MQ=255)
                                                                          aCTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGa                                                                          >  1:2405331/1‑149 (MQ=255)
                                                                          aCTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGa                                                                          <  1:4169454/149‑1 (MQ=255)
                                                                           cTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGAt                                                                         >  1:1363470/1‑149 (MQ=255)
                                                                           cTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGAt                                                                         <  2:4335337/149‑1 (MQ=255)
                                                                                aGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCaaa                                                                    <  2:1467305/149‑1 (MQ=255)
                                                                                 ggAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAAAATCCTGTCCAGCAATGGCTGGACAGGCGATGCaaaa                                                                   >  1:4073849/1‑149 (MQ=255)
                                                                                 ggAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCaaaa                                                                   >  1:3008216/1‑149 (MQ=255)
                                                                                  gAGTAACCTTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAAGATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAAc                                                                  <  2:637017/149‑1 (MQ=255)
                                                                                   aGTAACATTACCTGAAAAATATCCCACCCCAACGCCGCCACGATCAATATTACCCTGGGTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACa                                                                 <  2:3855052/149‑1 (MQ=255)
                                                                                        cATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAAt                                                            <  1:4024058/149‑1 (MQ=255)
                                                                                          ttACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGa                                                          <  1:1365216/149‑1 (MQ=255)
                                                                                           tACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGac                                                         >  1:2452771/1‑149 (MQ=255)
                                                                                            aCCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGaca                                                        <  1:5226281/149‑1 (MQ=255)
                                                                                            aCCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGaca                                                        <  2:5032740/149‑1 (MQ=255)
                                                                                            aCCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGaca                                                        <  2:5109168/149‑1 (MQ=255)
                                                                                             ccTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGacag                                                       <  1:2600195/149‑1 (MQ=255)
                                                                                              cTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGacaga                                                      >  1:2278811/1‑149 (MQ=255)
                                                                                                     atatCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGacagaaacagaa                                               >  1:2505969/1‑149 (MQ=255)
                                                                                                      tatCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAat                                              <  1:5110414/149‑1 (MQ=255)
                                                                                                      tatCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAat                                              <  1:3393633/149‑1 (MQ=255)
                                                                                                       atCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAatt                                             <  1:3778303/149‑1 (MQ=255)
                                                                                                          ccACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAattatt                                          <  2:2454710/149‑1 (MQ=255)
                                                                                                          ccACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAattatt                                          >  2:2027304/1‑149 (MQ=255)
                                                                                                          ccACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAattatt                                          <  2:5186890/149‑1 (MQ=255)
                                                                                                            aCCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATtct                                        >  1:3175602/1‑149 (MQ=255)
                                                                                                            aCCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTAGTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATtct                                        <  2:2715905/149‑1 (MQ=255)
                                                                                                             ccccAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATtctc                                       <  2:1246538/149‑1 (MQ=255)
                                                                                                                cAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTtc                                    >  2:4985125/1‑149 (MQ=255)
                                                                                                                 aaCGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTtca                                   <  1:1551314/149‑1 (MQ=255)
                                                                                                                  acgacgCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTtcat                                  >  1:4316654/1‑149 (MQ=255)
                                                                                                                   cgacgCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTtcatc                                 <  1:4897904/149‑1 (MQ=255)
                                                                                                                   cgacgCCAAGATAATTTTTACGCTGTTTACTGTTTCATCTTTTGCATGTGCTGTTTTTTACTTTTGATACATGGTCATTATCGTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACTGTATTATTCTCTtcatc                                 <  1:3781091/149‑1 (MQ=255)
                                                                                                                      cgCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGc                              <  2:107486/149‑1 (MQ=255)
                                                                                                                       gCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCt                             >  1:3578153/1‑149 (MQ=255)
                                                                                                                         cAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTa                           <  2:396662/149‑1 (MQ=255)
                                                                                                                         cAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTa                           <  1:465919/149‑1 (MQ=255)
                                                                                                                         cAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTCATGACAGAAACAGAATTAGTCTCTTCATCAGCTTa                           >  2:3817684/1‑149 (MQ=255)
                                                                                                                          aaGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAACACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTAc                          <  2:1596564/149‑1 (MQ=255)
                                                                                                                          aaGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTAc                          <  1:4300152/149‑1 (MQ=255)
                                                                                                                            gATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGt                        <  1:4659874/149‑1 (MQ=255)
                                                                                                                             aTCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGtt                       <  1:3221609/149‑1 (MQ=255)
                                                                                                                             aTCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGtt                       >  2:3628261/1‑149 (MQ=255)
                                                                                                                                   atTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTgg                 <  2:3491122/149‑1 (MQ=255)
                                                                                                                                     tACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGa               <  2:1401271/149‑1 (MQ=255)
                                                                                                                                     tACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGa               <  1:797191/149‑1 (MQ=255)
                                                                                                                                      aCCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGAt              <  2:2947266/149‑1 (MQ=255)
                                                                                                                                           ggTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCata         >  1:1217921/1‑149 (MQ=255)
                                                                                                                                            gTTACTTATTCCGCTTTTGCCGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCACTGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCatat        >  2:4824404/1‑149 (MQ=255)
                                                                                                                                            gTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCatat        <  1:4293971/149‑1 (MQ=255)
                                                                                                                                            gTTACTGAGTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCatat        <  1:104461/149‑1 (MQ=255)
                                                                                                                                               aCTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCAtatata     <  2:4238311/149‑1 (MQ=255)
                                                                                                                                                  gATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGtt  >  2:565753/1‑149 (MQ=255)
                                                                                                                                                  gATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGtt  >  2:3190965/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TTTAGCACATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTT  >  CP009273/147800‑148094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTTAGCACATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTC  >  CP009273/147799‑148095
                                                                                                                                                   |                                                                                                                                                     
ATTTAGCACATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGAT                                                                                                                                                     >  SRR19973084.2186157/1‑150 (MQ=60)
ATTTAGCACATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGAT                                                                                                                                                     >  SRR19973084.345482/1‑150 (MQ=60)
 TTTAGCACATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATT                                                                                                                                                    <  SRR19973084.1502326/150‑1 (MQ=60)
      CACATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCT                                                                                                                                               >  SRR19973084.1144978/1‑150 (MQ=60)
      CACATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCT                                                                                                                                               >  SRR19973084.3853674/1‑150 (MQ=60)
       ACATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTT                                                                                                                                              >  SRR19973084.5207121/1‑150 (MQ=60)
       ACATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTT                                                                                                                                              >  SRR19973084.5207812/1‑150 (MQ=60)
        CATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTT                                                                                                                                             >  SRR19973084.2840500/1‑150 (MQ=60)
        CATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTT                                                                                                                                             >  SRR19973084.2840545/1‑150 (MQ=60)
         ATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTT                                                                                                                                            >  SRR19973084.1428249/1‑150 (MQ=60)
         ATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTT                                                                                                                                            <  SRR19973084.2862953/150‑1 (MQ=60)
         ATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTT                                                                                                                                            <  SRR19973084.5224372/150‑1 (MQ=60)
           CGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGC                                                                                                                                          >  SRR19973084.800273/1‑150 (MQ=60)
            GCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCA                                                                                                                                         >  SRR19973084.1803238/1‑150 (MQ=60)
               AGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGT                                                                                                                                      >  SRR19973084.2780591/1‑150 (MQ=60)
                GTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTG                                                                                                                                     >  SRR19973084.4802095/1‑150 (MQ=60)
                  TCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCT                                                                                                                                   <  SRR19973084.5151215/150‑1 (MQ=60)
                     CTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTT                                                                                                                                >  SRR19973084.2209827/1‑150 (MQ=60)
                        TAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTT                                                                                                                             <  SRR19973084.1249962/150‑1 (MQ=60)
                          ATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTA                                                                                                                           >  SRR19973084.2039972/1‑150 (MQ=60)
                           GGCACAGTCCGATACCGTGATAGCGAACTCTTTACCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTAC                                                                                                                          <  SRR19973084.4414871/150‑1 (MQ=60)
                               CAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTG                                                                                                                      <  SRR19973084.964728/150‑1 (MQ=60)
                                 GTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGC                                                                                                                    <  SRR19973084.3713967/150‑1 (MQ=60)
                                    GGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAAC                                                                                                                 <  SRR19973084.1328357/150‑1 (MQ=60)
                                    GGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAAC                                                                                                                 >  SRR19973084.3654582/1‑150 (MQ=60)
                                    GGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAAC                                                                                                                 >  SRR19973084.426081/1‑150 (MQ=60)
                                    GGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAAC                                                                                                                 >  SRR19973084.3654925/1‑150 (MQ=60)
                                     GATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACA                                                                                                                >  SRR19973084.4027879/1‑150 (MQ=60)
                                     GATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACA                                                                                                                >  SRR19973084.4028032/1‑150 (MQ=60)
                                     GATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACA                                                                                                                >  SRR19973084.4161420/1‑150 (MQ=60)
                                        ACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGG                                                                                                             >  SRR19973084.4897873/1‑150 (MQ=60)
                                        ACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGG                                                                                                             >  SRR19973084.3118066/1‑150 (MQ=60)
                                         CAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGT                                                                                                            >  SRR19973084.2793493/1‑150 (MQ=60)
                                          AGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTC                                                                                                           >  SRR19973084.3456744/1‑150 (MQ=60)
                                          AGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTC                                                                                                           >  SRR19973084.3456513/1‑150 (MQ=60)
                                            TTATAGTGAACTCTTGTCCACCTGTTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAA                                                                                                         <  SRR19973084.724713/150‑1 (MQ=60)
                                            TGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAA                                                                                                         >  SRR19973084.666399/1‑150 (MQ=60)
                                             GATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAAT                                                                                                        <  SRR19973084.1492519/150‑1 (MQ=60)
                                             GATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAAT                                                                                                        >  SRR19973084.2060042/1‑150 (MQ=60)
                                             GATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAAT                                                                                                        >  SRR19973084.2060034/1‑150 (MQ=60)
                                              ATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATA                                                                                                       <  SRR19973084.1644436/150‑1 (MQ=60)
                                                AGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATC                                                                                                     >  SRR19973084.3197630/1‑150 (MQ=60)
                                                 GTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCC                                                                                                    >  SRR19973084.4625368/1‑150 (MQ=60)
                                                  TGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCT                                                                                                   <  SRR19973084.2607360/150‑1 (MQ=60)
                                                    AACTCTTTTCCACCTGGTTGATAACTTTCCGGAGTAACATTCCCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGT                                                                                                 <  SRR19973084.1827777/150‑1 (MQ=60)
                                                    AACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGT                                                                                                 <  SRR19973084.1827844/150‑1 (MQ=60)
                                                    AACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGT                                                                                                 <  SRR19973084.4191474/150‑1 (MQ=60)
                                                      CTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCC                                                                                               >  SRR19973084.4282510/1‑150 (MQ=60)
                                                      CTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCGGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCC                                                                                               >  SRR19973084.883596/1‑150 (MQ=60)
                                                       TCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCA                                                                                              <  SRR19973084.3546352/150‑1 (MQ=60)
                                                        CTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAG                                                                                             <  SRR19973084.2122372/150‑1 (MQ=60)
                                                         TTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGC                                                                                            >  SRR19973084.1615233/1‑150 (MQ=60)
                                                         TTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGC                                                                                            >  SRR19973084.3734123/1‑150 (MQ=60)
                                                         TTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGC                                                                                            >  SRR19973084.4242386/1‑150 (MQ=60)
                                                             CCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATG                                                                                        <  SRR19973084.2850050/150‑1 (MQ=60)
                                                              CACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGG                                                                                       >  SRR19973084.2478236/1‑150 (MQ=60)
                                                              CACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGG                                                                                       >  SRR19973084.2478128/1‑150 (MQ=60)
                                                                 CTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTG                                                                                    <  SRR19973084.2983521/150‑1 (MQ=60)
                                                                 CTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTG                                                                                    <  SRR19973084.3364209/150‑1 (MQ=60)
                                                                 CTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTG                                                                                    <  SRR19973084.3364267/150‑1 (MQ=60)
                                                                   GGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGA                                                                                  <  SRR19973084.4623930/150‑1 (MQ=60)
                                                                    GTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGAC                                                                                 >  SRR19973084.1729098/1‑150 (MQ=60)
                                                                     TTGATAACTTTCAGGAGTACCATTACCTGAAAAATATCCCACCCCCACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACA                                                                                <  SRR19973084.4254130/150‑1 (MQ=60)
                                                                       GATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGG                                                                              <  SRR19973084.3265349/150‑1 (MQ=60)
                                                                        ATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGC                                                                             >  SRR19973084.3910765/1‑150 (MQ=60)
                                                                        ATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGC                                                                             >  SRR19973084.857401/1‑150 (MQ=60)
                                                                        ATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGC                                                                             >  SRR19973084.3930577/1‑150 (MQ=60)
                                                                        ATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGC                                                                             >  SRR19973084.3910415/1‑150 (MQ=60)
                                                                        ATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCATGTCAAGCAATGGCTGGACAGGC                                                                             >  SRR19973084.1659409/1‑150 (MQ=60)
                                                                        ATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATAGTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGC                                                                             <  SRR19973084.1753366/150‑1 (MQ=60)
                                                                          AACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGA                                                                           >  SRR19973084.2415461/1‑150 (MQ=60)
                                                                           ACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGAT                                                                          >  SRR19973084.1369262/1‑150 (MQ=60)
                                                                           ACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGAT                                                                          >  SRR19973084.1369098/1‑150 (MQ=60)
                                                                           ACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGAT                                                                          >  SRR19973084.2323780/1‑150 (MQ=60)
                                                                           ACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGAT                                                                          <  SRR19973084.4186454/150‑1 (MQ=60)
                                                                                 AGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAAAATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAA                                                                    >  SRR19973084.4090439/1‑150 (MQ=60)
                                                                                 AGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAA                                                                    >  SRR19973084.3505173/1‑150 (MQ=60)
                                                                                 AGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAA                                                                    >  SRR19973084.1455688/1‑150 (MQ=60)
                                                                                 AGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAA                                                                    >  SRR19973084.3020461/1‑150 (MQ=60)
                                                                                  GGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAAC                                                                   <  SRR19973084.532924/150‑1 (MQ=60)
                                                                                  GGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCGGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAAC                                                                   <  SRR19973084.2332695/150‑1 (MQ=60)
                                                                                         CATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATG                                                            <  SRR19973084.4040395/150‑1 (MQ=60)
                                                                                           TTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGAC                                                          >  SRR19973084.2463067/1‑150 (MQ=60)
                                                                                           TTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGAC                                                          <  SRR19973084.1370862/150‑1 (MQ=60)
                                                                                           TTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGAC                                                          >  SRR19973084.5005205/1‑150 (MQ=60)
                                                                                           TTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGAC                                                          >  SRR19973084.2463073/1‑150 (MQ=60)
                                                                                             ACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAG                                                        <  SRR19973084.5247315/150‑1 (MQ=60)
                                                                                              CCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGA                                                       >  SRR19973084.2288364/1‑150 (MQ=60)
                                                                                              CCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGA                                                       <  SRR19973084.2610898/150‑1 (MQ=60)
                                                                                                 GAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAAC                                                    >  SRR19973084.3406504/1‑150 (MQ=60)
                                                                                                   AAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAG                                                  <  SRR19973084.1753892/150‑1 (MQ=60)
                                                                                                     AATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAA                                                >  SRR19973084.2516440/1‑150 (MQ=60)
                                                                                                       TATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATT                                              <  SRR19973084.3133265/150‑1 (MQ=60)
                                                                                                       TATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATT                                              <  SRR19973084.5131238/150‑1 (MQ=60)
                                                                                                       TATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATT                                              <  SRR19973084.5131162/150‑1 (MQ=60)
                                                                                                       TATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATT                                              <  SRR19973084.3407481/150‑1 (MQ=60)
                                                                                                        ATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTA                                             <  SRR19973084.3793655/150‑1 (MQ=60)
                                                                                                          CCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATT                                           <  SRR19973084.4942638/150‑1 (MQ=60)
                                                                                                           CCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTC                                          >  SRR19973084.4704539/1‑150 (MQ=60)
                                                                                                            CACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCT                                         <  SRR19973084.4759212/150‑1 (MQ=60)
                                                                                                            CACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCT                                         >  SRR19973084.3188718/1‑150 (MQ=60)
                                                                                                            CACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCT                                         <  SRR19973084.4325846/150‑1 (MQ=60)
                                                                                                                  AACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCAT                                   >  SRR19973084.4334130/1‑150 (MQ=60)
                                                                                                                  AACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCAT                                   >  SRR19973084.4670180/1‑150 (MQ=60)
                                                                                                                  AACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCAT                                   <  SRR19973084.1557944/150‑1 (MQ=60)
                                                                                                                  AACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCAT                                   <  SRR19973084.1557912/150‑1 (MQ=60)
                                                                                                                    CGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCA                                 <  SRR19973084.4079154/150‑1 (MQ=60)
                                                                                                                    CGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCA                                 <  SRR19973084.4312533/150‑1 (MQ=60)
                                                                                                                    CGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCA                                 <  SRR19973084.3796355/150‑1 (MQ=60)
                                                                                                                    CGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCA                                 <  SRR19973084.4917726/150‑1 (MQ=60)
                                                                                                                    CGACGCCAAGATAATTTTTACGCTGTTTACTGTTTCATCTTTTGCATGTGCTGTTTTTTACTTTTGATACATGGTCATTATCGTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACTGTATTATTCTCTTCATCA                                 <  SRR19973084.3796448/150‑1 (MQ=56)
                                                                                                                       CGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCT                              >  SRR19973084.3592763/1‑150 (MQ=60)
                                                                                                                         CCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTA                            <  SRR19973084.4065681/150‑1 (MQ=60)
                                                                                                                          CAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTAC                           <  SRR19973084.467796/150‑1 (MQ=60)
                                                                                                                           AAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACG                          <  SRR19973084.4317604/150‑1 (MQ=60)
                                                                                                                           AAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACG                          <  SRR19973084.4317546/150‑1 (MQ=60)
                                                                                                                             GATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTT                        <  SRR19973084.4678818/150‑1 (MQ=60)
                                                                                                                             GATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTT                        <  SRR19973084.4678684/150‑1 (MQ=60)
                                                                                                                              ATCAATATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTG                       <  SRR19973084.3234945/150‑1 (MQ=60)
                                                                                                                              ATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTG                       >  SRR19973084.3580364/1‑150 (MQ=60)
                                                                                                                              ATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTG                       >  SRR19973084.3580030/1‑150 (MQ=60)
                                                                                                                              ATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTG                       >  SRR19973084.3580341/1‑150 (MQ=60)
                                                                                                                                 AATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGT                    >  SRR19973084.4359190/1‑150 (MQ=60)
                                                                                                                                    ATTACCCTGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGG                 <  SRR19973084.3981826/150‑1 (MQ=60)
                                                                                                                                      TACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGAT               <  SRR19973084.800527/150‑1 (MQ=60)
                                                                                                                                      TACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGAT               >  SRR19973084.2102925/1‑150 (MQ=60)
                                                                                                                                           TGGTTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATA          >  SRR19973084.1222983/1‑150 (MQ=60)
                                                                                                                                             GTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATA        <  SRR19973084.4311401/150‑1 (MQ=60)
                                                                                                                                             GTTACTGAGTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATN        <  SRR19973084.105108/150‑1 (MQ=60)
                                                                                                                                              TTACTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATAT       >  SRR19973084.5185711/1‑150 (MQ=60)
                                                                                                                                                 CTTATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGT    >  SRR19973084.4312998/1‑150 (MQ=60)
                                                                                                                                                   TATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTC  <  SRR19973084.2324039/150‑1 (MQ=60)
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ATTTAGCACATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTC  >  CP009273/147799‑148095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑