Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,073,243 C→A 37.3% G366C (GGT→TGT)  putA ← fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,073,2430CA37.3% 372.9 / 271.0 260G366C (GGT→TGT) putAfused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (101/62);  new base A (51/46);  total (152/108)
Fisher's exact test for biased strand distribution p-value = 1.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 265 total aligned reads displayed.

ATCGATCACCAACGGGCAGCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGATGCCACGACCG  >  CP009273/1073094‑1073391
                                                                                                                                                     |                                                                                                                                                    
  cGATCACCAACGGGCAGCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCaa                                                                                                                                                    <  1:2630158/150‑1 (MQ=255)
    aTCACCAACGGGCATCTTTTTTGATAAGCCTGAATAACACAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTAGGCGTCATTGTTGATACAAata                                                                                                                                                  <  1:353295/150‑1 (MQ=255)
    aTCACCAACGGGCAGCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAata                                                                                                                                                  <  1:353355/150‑1 (MQ=255)
     tCACCAACGGGCAGCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAatat                                                                                                                                                 >  1:3337221/1‑150 (MQ=255)
      cACCAACGGGCAGCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATc                                                                                                                                                <  2:2833086/150‑1 (MQ=255)
       aCCAACGGGCAGCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCg                                                                                                                                               >  1:1043817/1‑150 (MQ=255)
       aCCAACGGGCAGCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCg                                                                                                                                               >  1:458868/1‑150 (MQ=255)
        ccAACGGGCAGCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGt                                                                                                                                              <  1:756023/150‑1 (MQ=255)
          aaCGGGCAGCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTAc                                                                                                                                            <  1:3756260/150‑1 (MQ=255)
          aaCGGGCAGCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTAc                                                                                                                                            >  1:1316703/1‑150 (MQ=255)
          aaCGGGCAGCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTAc                                                                                                                                            >  1:2692466/1‑150 (MQ=255)
             gggCAGCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGa                                                                                                                                         >  1:3037100/1‑150 (MQ=255)
                  gCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACgcgc                                                                                                                                    >  1:1842567/1‑150 (MQ=255)
                   cGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGcc                                                                                                                                   >  1:2167670/1‑150 (MQ=255)
                    gTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCca                                                                                                                                  >  2:1172607/1‑150 (MQ=255)
                    gTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCca                                                                                                                                  <  1:2457857/150‑1 (MQ=255)
                    gTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCca                                                                                                                                  <  1:608610/150‑1 (MQ=255)
                      ttttGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCcagc                                                                                                                                >  2:756659/1‑150 (MQ=255)
                             aaGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGgtga                                                                                                                         >  2:274823/1‑150 (MQ=255)
                             aaGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGgtga                                                                                                                         <  2:1750415/150‑1 (MQ=255)
                               gCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGgtgagt                                                                                                                       <  1:2239221/150‑1 (MQ=255)
                                  tGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGAt                                                                                                                    <  1:3823660/150‑1 (MQ=255)
                                      tAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCa                                                                                                                <  1:4036869/150‑1 (MQ=255)
                                       aaCAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAg                                                                                                               <  2:160058/150‑1 (MQ=255)
                                       aaCAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAg                                                                                                               <  1:1384143/150‑1 (MQ=255)
                                         cAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGAc                                                                                                             <  2:516424/150‑1 (MQ=255)
                                          aaaaCCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGAcg                                                                                                            <  1:848758/150‑1 (MQ=255)
                                            aaCCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGAcgcg                                                                                                          >  2:2991144/1‑150 (MQ=255)
                                              ccGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCggg                                                                                                        >  1:3194885/1‑150 (MQ=255)
                                               cGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGt                                                                                                       >  1:2558269/1‑150 (MQ=255)
                                               cGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGt                                                                                                       >  2:1005997/1‑150 (MQ=255)
                                                gATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTa                                                                                                      >  2:1989136/1‑150 (MQ=255)
                                                         ccAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTc                                                                                             >  1:938376/1‑150 (MQ=255)
                                                         ccAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCACGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTc                                                                                             >  1:938656/1‑150 (MQ=255)
                                                           aGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCa                                                                                           <  2:1295339/150‑1 (MQ=255)
                                                           aGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCa                                                                                           >  1:125220/1‑150 (MQ=255)
                                                             ccTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCAtt                                                                                         <  2:3742566/150‑1 (MQ=255)
                                                                gCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTAcc                                                                                      >  2:1032236/1‑150 (MQ=255)
                                                                    gTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGt                                                                                  <  2:1132154/150‑1 (MQ=255)
                                                                    gTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGt                                                                                  <  2:3904438/150‑1 (MQ=255)
                                                                    gTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGt                                                                                  <  1:849173/150‑1 (MQ=255)
                                                                           cTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTg                                                                           >  2:3798820/1‑150 (MQ=255)
                                                                            tCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTgg                                                                          >  2:2287648/1‑150 (MQ=255)
                                                                               aaaCAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGgcg                                                                       >  2:3208502/1‑150 (MQ=255)
                                                                               aaaCAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGgcg                                                                       <  1:752463/150‑1 (MQ=255)
                                                                                  cAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCgg                                                                    >  2:1637695/1‑150 (MQ=255)
                                                                                  cAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCgg                                                                    >  2:3058301/1‑150 (MQ=255)
                                                                                  cAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCgg                                                                    >  2:1888657/1‑150 (MQ=255)
                                                                                   agagTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGc                                                                   >  1:77671/1‑150 (MQ=255)
                                                                                     agTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCta                                                                 <  2:3315216/150‑1 (MQ=255)
                                                                                         tttCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAAc                                                             <  2:2480118/150‑1 (MQ=255)
                                                                                         tttCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAAc                                                             >  2:3379936/1‑150 (MQ=255)
                                                                                         tttCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCAGTACCCGGTCATACTGGGCGCGGCTATAAc                                                             >  1:2730649/1‑150 (MQ=255)
                                                                                             cagcagATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCgg                                                         <  2:2130841/150‑1 (MQ=255)
                                                                                              agcagATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGa                                                        >  1:2100731/1‑150 (MQ=255)
                                                                                              agcagATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGa                                                        >  2:4006339/1‑150 (MQ=255)
                                                                                              agcagATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGa                                                        >  1:565992/1‑150 (MQ=255)
                                                                                                cagATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATg                                                      >  1:2396532/1‑150 (MQ=255)
                                                                                                cagATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATg                                                      <  1:3618732/150‑1 (MQ=255)
                                                                                                 cgATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGc                                                     >  2:656150/2‑150 (MQ=255)
                                                                                                  gATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGCTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCa                                                    >  1:4107642/1‑150 (MQ=255)
                                                                                                   aTCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAg                                                   <  2:1840219/150‑1 (MQ=255)
                                                                                                   aTCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAg                                                   >  1:3426277/1‑150 (MQ=255)
                                                                                                      gAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAgcgc                                                >  1:712357/1‑150 (MQ=255)
                                                                                                            gaTCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAg                                          >  1:2931700/1‑150 (MQ=255)
                                                                                                            gaTCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAg                                          >  2:1236643/1‑150 (MQ=255)
                                                                                                            gaTCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAg                                          >  1:2463194/1‑150 (MQ=255)
                                                                                                              tctcCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGtt                                        <  2:2517653/150‑1 (MQ=255)
                                                                                                              tctcCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGtt                                        <  1:3695106/150‑1 (MQ=255)
                                                                                                               ctcCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGttt                                       <  2:2020587/150‑1 (MQ=255)
                                                                                                                tcCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTttg                                      >  1:140650/1‑150 (MQ=255)
                                                                                                                   aGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCTTTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTttgatt                                   >  2:3115225/1‑150 (MQ=255)
                                                                                                                   aGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTttgatt                                   <  2:2741408/150‑1 (MQ=255)
                                                                                                                   aGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTttgatt                                   >  1:1690209/1‑150 (MQ=255)
                                                                                                                    ggCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTttgattg                                  >  2:237131/1‑150 (MQ=255)
                                                                                                                      cGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGaa                                >  1:2344739/1‑150 (MQ=255)
                                                                                                                       gATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGaaa                               >  2:1389466/1‑150 (MQ=255)
                                                                                                                       gATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGaaa                               >  2:3061961/1‑150 (MQ=255)
                                                                                                                        aTCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAAt                              >  1:505709/1‑150 (MQ=255)
                                                                                                                          cGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGc                            >  1:1987951/1‑150 (MQ=255)
                                                                                                                           ggACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGcc                           <  2:4053068/150‑1 (MQ=255)
                                                                                                                             aCTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCg                         >  2:1791046/1‑150 (MQ=255)
                                                                                                                             aCTCTTCGGCGTCAATGTTGATACAAATATCGTACTGTCGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATTCTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCg                         <  2:1309369/150‑1 (MQ=255)
                                                                                                                              ctctTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCgg                        >  1:3056394/1‑150 (MQ=255)
                                                                                                                               tctTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGc                       >  1:4166816/1‑150 (MQ=255)
                                                                                                                                  tCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCt                    <  1:1068751/150‑1 (MQ=255)
                                                                                                                                    ggCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTc                  >  1:3458416/1‑150 (MQ=255)
                                                                                                                                    ggCGTCAATGTTGATACCAAAATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCCCCGACAGTATGATAGAAATGCCCGGCCCtac                  >  1:225089/1‑148 (MQ=255)
                                                                                                                                      cGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCAt                >  2:1440151/1‑150 (MQ=255)
                                                                                                                                         cAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGa             <  1:2485912/150‑1 (MQ=255)
                                                                                                                                          aaTGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTGGATTGAAATGCCCGGCCCTTCATAGAt            >  2:345889/1‑150 (MQ=255)
                                                                                                                                          aaTGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGAt            >  1:2813615/1‑150 (MQ=255)
                                                                                                                                          aaTGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGAt            >  1:1739085/1‑150 (MQ=255)
                                                                                                                                           aTGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGATg           <  2:2617107/150‑1 (MQ=255)
                                                                                                                                               tGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGATGCCAc       >  2:3094542/1‑150 (MQ=255)
                                                                                                                                                gATACCAATCTCGTACTGAAGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTATAGCTCTTCCATTACCCGGTCATACTGGGCGCGTCTATAACGCGGATGCAGCGCCGAAAGTTTGATTGAAATGCCCGGCCCTTCATAGATGCCACg      <  1:898991/150‑1 (MQ=255)
                                                                                                                                                gATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGATGCCACg      <  2:223398/150‑1 (MQ=255)
                                                                                                                                                 aTACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGATGCCACGa     >  2:292623/1‑150 (MQ=255)
                                                                                                                                                   aCCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGATGCCACGAcc   <  2:3542957/150‑1 (MQ=255)
                                                                                                                                                   aCCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGATGCCACGAcc   <  2:3543119/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATCGATCACCAACGGGCAGCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGATGCCACGACCG  >  CP009273/1073094‑1073391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A