Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F204 I0 R1	1155	255.8	8356456	95.7%	7997128	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,396,082	G→T	100%	R204S (CGT→AGT)	csrD ←	targeting factor for csrBC sRNA degradation

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,396,082	0	G	T	99.7%	864.0 / ‑6.3	249	R204S (CGT→AGT)	csrD	targeting factor for csrBC sRNA degradation
Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (145/100); minor base A (4/0); total (149/100)
Fisher's exact test for biased strand distribution p-value = 1.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 249 total aligned reads displayed.

CTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCAGCGTA  >  CP009273/3395935‑3396228

cTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTaa                                                                                                                                                  >  1:1325390/1‑150 (MQ=255)

 tACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAag                                                                                                                                                 <  2:3701182/150‑1 (MQ=255)

    tttCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAagcag                                                                                                                                              <  1:2622826/150‑1 (MQ=255)

     ttCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAagcagc                                                                                                                                             <  2:2664916/150‑1 (MQ=255)

     ttCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAagcagc                                                                                                                                             >  2:1951406/1‑150 (MQ=255)

         ccTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTAt                                                                                                                                         <  2:44541/150‑1 (MQ=255)

         ccTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTAt                                                                                                                                         <  2:1228209/150‑1 (MQ=255)

         ccTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTAt                                                                                                                                         <  2:3122457/150‑1 (MQ=255)

         ccTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTAt                                                                                                                                         <  2:1227218/150‑1 (MQ=255)

          cTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTAACTAAGCAGCGTATc                                                                                                                                        >  1:2998282/1‑150 (MQ=255)

               cTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGGTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTCTCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAgcg                                                                                                                                   >  2:3719928/1‑150 (MQ=255)

                  ccAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCAc                                                                                                                                <  2:2173976/150‑1 (MQ=255)

                  ccAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCAc                                                                                                                                >  1:3215254/1‑150 (MQ=255)

                    aGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCActg                                                                                                                              >  2:2571318/1‑150 (MQ=255)

                      tAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCActgct                                                                                                                            <  2:1412239/150‑1 (MQ=255)

                       aaCGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCActgctg                                                                                                                           >  2:3090703/1‑150 (MQ=255)

                          gTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGtt                                                                                                                        >  1:243586/1‑150 (MQ=255)

                              cTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTgg                                                                                                                    >  1:3109082/1‑150 (MQ=255)

                              cTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCAAAAGATCGGATCAGCGTATCAAGACGGCTGTTTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCAGGTTCTgg                                                                                                                    >  1:2940710/1‑150 (MQ=255)

                               tAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACCTCCAGGGCGGCATAAGAGCGGATAAGCGTATAAACAAGGCTGTGTAGTTCGCGTGCGTTATTAATTTAACAAAGCAGCGTATACAGCGCACAGCTGGTTCAggg                                                                                                                   >  1:1815235/1‑150 (MQ=255)

                               tAACTGATTATCGAAAAAGAGTCGGTTATTAAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCACCGCACTGCTGGTTCTggg                                                                                                                   >  1:3860646/1‑150 (MQ=255)

                                aaCTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGggc                                                                                                                  >  1:2335939/1‑150 (MQ=255)

                                aaCTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGggc                                                                                                                  >  2:2891593/1‑150 (MQ=255)

                                 aCTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGggcg                                                                                                                 >  2:2773456/1‑150 (MQ=255)

                                 aCTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGggcg                                                                                                                 <  1:1924730/150‑1 (MQ=255)

                                    gATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGcc                                                                                                              >  2:45222/1‑150 (MQ=255)

                                    gATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTAGCCTTCGTTCTGAATTACACTAAGCAGCGTATCCAGCGCACTGCTGGTACTGGGCGGcc                                                                                                              >  2:919413/1‑150 (MQ=255)

                                    gATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCAGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGGTCTGGGCGGcc                                                                                                              >  2:2066929/1‑150 (MQ=255)

                                     aTTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCa                                                                                                             <  2:1976300/150‑1 (MQ=255)

                                       tATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCGGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCAtt                                                                                                           >  2:2524192/1‑150 (MQ=255)

                                          cGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCAt                                                                                                        >  1:852159/1‑150 (MQ=255)

                                          cGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCAt                                                                                                        >  1:275327/1‑150 (MQ=255)

                                           gAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATa                                                                                                       >  1:2080808/1‑150 (MQ=255)

                                            aaaaaGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAg                                                                                                      >  1:3927854/1‑150 (MQ=255)

                                             aaaaGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGa                                                                                                     >  1:3359728/1‑150 (MQ=255)

                                              aaaGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGAt                                                                                                    <  2:1446791/150‑1 (MQ=255)

                                              aaaGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGAt                                                                                                    <  2:3021157/150‑1 (MQ=255)

                                              aaaGAGTCGGTTATTGAGGCCGGTTTTCAAGTCCTGGGCGGCATAAGAGCGGATCAGCGTAACAATACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGTCGGCCATTCATAGAt                                                                                                    >  2:2354378/1‑150 (MQ=255)

                                                 gagTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGt                                                                                                 >  1:90638/1‑150 (MQ=255)

                                                  agTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGtt                                                                                                <  2:2496218/150‑1 (MQ=255)

                                                     cGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTccc                                                                                             >  2:3654616/1‑150 (MQ=255)

                                                     cGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTccc                                                                                             >  2:564170/1‑150 (MQ=255)

                                                     cGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTccc                                                                                             >  1:2828605/1‑150 (MQ=255)

                                                        ttATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAac                                                                                          >  1:3972720/1‑150 (MQ=255)

                                                           ttGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAAcaca                                                                                       >  1:606393/1‑150 (MQ=255)

                                                              aGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTa                                                                                    >  1:102087/1‑150 (MQ=255)

                                                              aGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTACTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTa                                                                                    >  2:487365/1‑150 (MQ=255)

                                                               ggCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTag                                                                                   <  1:2514023/150‑1 (MQ=255)

                                                                  cGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGc                                                                                >  1:943696/1‑150 (MQ=255)

                                                                  cGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGc                                                                                >  2:1502030/1‑150 (MQ=255)

                                                                  cGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGc                                                                                >  2:203321/1‑150 (MQ=255)

                                                                  cGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGc                                                                                >  2:2203919/1‑150 (MQ=255)

                                                                   ggTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGcc                                                                               <  2:868709/150‑1 (MQ=255)

                                                                   ggTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGcc                                                                               <  2:2970016/150‑1 (MQ=255)

                                                                    gTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCa                                                                              >  1:1237870/1‑150 (MQ=255)

                                                                     ttttCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCAc                                                                             <  1:2812541/150‑1 (MQ=255)

                                                                        tCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCt                                                                          >  2:402007/1‑150 (MQ=255)

                                                                         cACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTc                                                                         <  1:2758368/150‑1 (MQ=255)

                                                                             tCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCg                                                                     >  1:2458380/1‑150 (MQ=255)

                                                                              ccTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGt                                                                    >  2:2996787/1‑150 (MQ=255)

                                                                              ccTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGt                                                                    >  1:4019346/1‑150 (MQ=255)

                                                                                tGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGttt                                                                  <  1:3204030/150‑1 (MQ=255)

                                                                                 gggCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTa                                                                 <  1:3536098/150‑1 (MQ=255)

                                                                                 gggCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTa                                                                 <  1:3249578/150‑1 (MQ=255)

                                                                                 gggCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTa                                                                 >  1:217233/1‑150 (MQ=255)

                                                                                     ggcATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGAt                                                             >  1:698812/1‑150 (MQ=255)

                                                                                         tAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGa                                                         <  2:3165863/150‑1 (MQ=255)

                                                                                         tAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGa                                                         >  2:112622/1‑150 (MQ=255)

                                                                                          aaGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAg                                                        >  2:2553332/1‑150 (MQ=255)

                                                                                               cGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGcc                                                   >  1:1992175/1‑150 (MQ=255)

                                                                                               cGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGcc                                                   >  1:1525479/1‑150 (MQ=255)

                                                                                                   tCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCggg                                               >  2:3884415/1‑150 (MQ=255)

                                                                                                        gTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTcc                                          >  2:3591416/1‑150 (MQ=255)

                                                                                                          aTCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAg                                        >  2:2112644/1‑150 (MQ=255)

                                                                                                            cAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCa                                      <  1:3298126/150‑1 (MQ=255)

                                                                                                             aaGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCaa                                     >  1:3733995/1‑150 (MQ=255)

                                                                                                             aaGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCaa                                     >  2:2693534/1‑150 (MQ=255)

                                                                                                                  ggCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCtt                                <  1:3959352/150‑1 (MQ=255)

                                                                                                                         ttgttCGCGTGCGTTCTGAATTTCACTACGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTCAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGc                         <  1:2628823/150‑1 (MQ=255)

                                                                                                                            ttCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAg                      <  1:3658184/150‑1 (MQ=255)

                                                                                                                               gcgtgcgtTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTg                   <  1:3544943/150‑1 (MQ=255)

                                                                                                                                cgtgcgtTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGc                  >  1:801854/1‑150 (MQ=255)

                                                                                                                                 gtgcgtTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGcc                 >  2:1632957/1‑150 (MQ=255)

                                                                                                                                 gtgcgtTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGcc                 >  2:31420/1‑150 (MQ=255)

                                                                                                                                 gtgcgtTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGcc                 <  2:3482968/150‑1 (MQ=255)

                                                                                                                                 gtgcgtTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGcc                 <  2:2739280/150‑1 (MQ=255)

                                                                                                                                  tgcgtTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTCGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCg                >  2:3224294/1‑150 (MQ=255)

                                                                                                                                   gcgtTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGt               <  1:1566136/150‑1 (MQ=255)

                                                                                                                                   gcgtTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGt               <  2:1897568/150‑1 (MQ=255)

                                                                                                                                     gtTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTg             >  2:1401747/1‑150 (MQ=255)

                                                                                                                                      ttCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGt            >  2:1763013/1‑150 (MQ=255)

                                                                                                                                      ttCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTGGt            >  2:1763004/1‑150 (MQ=255)

                                                                                                                                         tGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAAc         <  2:3780549/150‑1 (MQ=255)

                                                                                                                                         tGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAAc         <  2:2926090/150‑1 (MQ=255)

                                                                                                                                         tGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAAc         >  1:3873699/1‑150 (MQ=255)

                                                                                                                                         tGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAAc         >  1:537633/1‑150 (MQ=255)

                                                                                                                                          gAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCACGTTGCCGTTGTAAcc        >  2:3427275/1‑150 (MQ=255)

                                                                                                                                          gAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAAcc        <  2:3828589/150‑1 (MQ=255)

                                                                                                                                          gAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAAcc        <  2:237872/150‑1 (MQ=255)

                                                                                                                                                cACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCAGCGTa  >  1:2255877/1‑150 (MQ=255)

CTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCAGCGTA  >  CP009273/3395935‑3396228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCAGCGTACC  >  CP009273/3395933‑3396230

ACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACT                                                                                                                                                      >  SRR19973074.2299516/1‑150 (MQ=60)

ACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACT                                                                                                                                                      <  SRR19973074.1429044/150‑1 (MQ=60)

  CTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAA                                                                                                                                                    >  SRR19973074.1327874/1‑150 (MQ=60)

  CTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAA                                                                                                                                                    >  SRR19973074.1548576/1‑150 (MQ=60)

   TACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAG                                                                                                                                                   >  SRR19973074.622908/1‑150 (MQ=60)

      TTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAG                                                                                                                                                <  SRR19973074.2627951/150‑1 (MQ=60)

      TTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAG                                                                                                                                                <  SRR19973074.2627731/150‑1 (MQ=60)

      TTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAG                                                                                                                                                <  SRR19973074.4065233/150‑1 (MQ=60)

        TCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCG                                                                                                                                              >  SRR19973074.925127/1‑150 (MQ=60)

          TCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTA                                                                                                                                            <  SRR19973074.1573714/150‑1 (MQ=60)

          TCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTA                                                                                                                                            <  SRR19973074.3371989/150‑1 (MQ=60)

            CTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATC                                                                                                                                          >  SRR19973074.2936043/1‑150 (MQ=60)

            CTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTAACTAAGCAGCGTATC                                                                                                                                          >  SRR19973074.3003849/1‑150 (MQ=60)

            CTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGGTCTGAATTTCACTAAGCAGCGTATC                                                                                                                                          >  SRR19973074.3248327/1‑150 (MQ=60)

             TGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCC                                                                                                                                         <  SRR19973074.2773783/150‑1 (MQ=60)

               ATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAG                                                                                                                                       >  SRR19973074.3656909/1‑150 (MQ=60)

                    CCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCAC                                                                                                                                  >  SRR19973074.3221194/1‑150 (MQ=60)

                     CAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACT                                                                                                                                 <  SRR19973074.1421040/150‑1 (MQ=60)

                            GTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTT                                                                                                                          >  SRR19973074.2610164/1‑150 (MQ=60)

                            GTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTT                                                                                                                          >  SRR19973074.244072/1‑150 (MQ=60)

                              TGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCT                                                                                                                        <  SRR19973074.703752/150‑1 (MQ=60)

                              TGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCT                                                                                                                        <  SRR19973074.1962561/150‑1 (MQ=60)

                               GCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTG                                                                                                                       <  SRR19973074.3595904/150‑1 (MQ=60)

                                CTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGG                                                                                                                      >  SRR19973074.3114789/1‑150 (MQ=60)

                                CTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCAAAAGATCGGATCAGCGTATCAAGACGGCTGTTTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCAGGTTCTGG                                                                                                                      >  SRR19973074.2946179/1‑150 (MQ=60)

                                CTAACTGATTATCGAAAAAGAGTCGGTTATTGAAGCCGGTATAAACGACCAGGGCGCCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGGGCCTTCTGAATTTCAAAAAGCAGCGAATACAGCGCACTGCTGGTACTGG                                                                                                                      >  SRR19973074.2324686/1‑150 (MQ=60)

                                 TAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACCTCCAGGGCGGCATAAGAGCGGATAAGCGTATAAACAAGGCTGTGTAGTTCGCGTGCGTTATTAATTTAACAAAGCAGCGTATACAGCGCACAGCTGGTTCAGGG                                                                                                                     >  SRR19973074.1818691/1‑150 (MQ=60)

                                 TAACTGATTATCGAAAAAGAGTCGGTTATTAAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCACCGCACTGCTGGTTCTGGG                                                                                                                     >  SRR19973074.3867694/1‑150 (MQ=60)

                                  AACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGC                                                                                                                    >  SRR19973074.2340295/1‑150 (MQ=60)

                                   ACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCG                                                                                                                   <  SRR19973074.1928417/150‑1 (MQ=60)

                                            CGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCAT                                                                                                          >  SRR19973074.275851/1‑150 (MQ=60)

                                            CGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCAT                                                                                                          >  SRR19973074.275810/1‑150 (MQ=60)

                                            CGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCAT                                                                                                          >  SRR19973074.853800/1‑150 (MQ=60)

                                            CGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCAT                                                                                                          >  SRR19973074.853794/1‑150 (MQ=60)

                                             GAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATA                                                                                                         >  SRR19973074.2084704/1‑150 (MQ=60)

                                             GAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATA                                                                                                         <  SRR19973074.2876051/150‑1 (MQ=60)

                                             GAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTAGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATA                                                                                                         >  SRR19973074.2084719/1‑150 (MQ=60)

                                              AAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAG                                                                                                        >  SRR19973074.3935081/1‑150 (MQ=60)

                                               AAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGA                                                                                                       >  SRR19973074.3718438/1‑150 (MQ=60)

                                               AAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGA                                                                                                       >  SRR19973074.3365961/1‑150 (MQ=60)

                                                   GAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGT                                                                                                   >  SRR19973074.90861/1‑150 (MQ=60)

                                                       CGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCC                                                                                               >  SRR19973074.3936614/1‑150 (MQ=60)

                                                       CGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCC                                                                                               >  SRR19973074.3043469/1‑150 (MQ=60)

                                                       CGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCC                                                                                               >  SRR19973074.3043539/1‑150 (MQ=60)

                                                       CGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCC                                                                                               >  SRR19973074.2833838/1‑150 (MQ=60)

                                                        GGTTTTTGAGTCCGGTTTTCACGTCCTGGGCGTCATAAGATCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCA                                                                                              <  SRR19973074.11933/150‑1 (MQ=60)

                                                        GGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCA                                                                                              >  SRR19973074.2458368/1‑150 (MQ=60)

                                                          TTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAAC                                                                                            >  SRR19973074.3980043/1‑150 (MQ=60)

                                                           TATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTNTGGGCGGCCATTCATAGATGGTTCCCAACA                                                                                           <  SRR19973074.3860521/150‑1 (MQ=60)

                                                             TTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACA                                                                                         >  SRR19973074.607547/1‑150 (MQ=60)

                                                                AGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTA                                                                                      >  SRR19973074.102337/1‑150 (MQ=60)

                                                                 GGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAG                                                                                     <  SRR19973074.2518713/150‑1 (MQ=60)

                                                                    CGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGC                                                                                  >  SRR19973074.945492/1‑150 (MQ=60)

                                                                      GTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCA                                                                                >  SRR19973074.1240239/1‑150 (MQ=60)

                                                                       TTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCAC                                                                               <  SRR19973074.2817686/150‑1 (MQ=60)

                                                                       TTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCAC                                                                               <  SRR19973074.2817738/150‑1 (MQ=60)

                                                                        TTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACG                                                                              >  SRR19973074.3760653/1‑150 (MQ=60)

                                                                           CACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTC                                                                           <  SRR19973074.2763447/150‑1 (MQ=60)

                                                                           CACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTC                                                                           <  SRR19973074.891084/150‑1 (MQ=60)

                                                                             CGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCAC                                                                         >  SRR19973074.1988991/1‑150 (MQ=60)

                                                                               TCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCG                                                                       >  SRR19973074.2462926/1‑150 (MQ=60)

                                                                               TCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCG                                                                       >  SRR19973074.2463312/1‑150 (MQ=60)

                                                                                CCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGT                                                                      >  SRR19973074.4026777/1‑150 (MQ=60)

                                                                                 CTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTT                                                                     <  SRR19973074.2298467/150‑1 (MQ=60)

                                                                                  TGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTT                                                                    <  SRR19973074.3209937/150‑1 (MQ=60)

                                                                                  TGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTT                                                                    <  SRR19973074.3209942/150‑1 (MQ=60)

                                                                                  TGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTT                                                                    <  SRR19973074.3209970/150‑1 (MQ=60)

                                                                                  TGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTT                                                                    >  SRR19973074.607406/1‑150 (MQ=60)

                                                                                   GGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTA                                                                   <  SRR19973074.3255591/150‑1 (MQ=60)

                                                                                   GGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTA                                                                   <  SRR19973074.3255594/150‑1 (MQ=60)

                                                                                   GGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTA                                                                   >  SRR19973074.217679/1‑150 (MQ=60)

                                                                                   GGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTA                                                                   >  SRR19973074.217680/1‑150 (MQ=60)

                                                                                   GGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTA                                                                   <  SRR19973074.4011818/150‑1 (MQ=60)

                                                                                   GGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTA                                                                   <  SRR19973074.3542613/150‑1 (MQ=60)

                                                                                   GGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTA                                                                   <  SRR19973074.3542621/150‑1 (MQ=60)

                                                                                       GGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGAT                                                               >  SRR19973074.1252405/1‑150 (MQ=60)

                                                                                       GGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGAT                                                               >  SRR19973074.700104/1‑150 (MQ=60)

                                                                                                 CGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCC                                                     >  SRR19973074.1995956/1‑150 (MQ=60)

                                                                                                 CGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCC                                                     >  SRR19973074.1528360/1‑150 (MQ=60)

                                                                                                    ATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGG                                                  >  SRR19973074.3314978/1‑150 (MQ=60)

                                                                                                     TCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGG                                                 >  SRR19973074.282870/1‑150 (MQ=60)

                                                                                                        GCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTT                                              >  SRR19973074.2394250/1‑150 (MQ=60)

                                                                                                          GTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCC                                            >  SRR19973074.3898901/1‑150 (MQ=60)

                                                                                                          GTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCC                                            >  SRR19973074.3898900/1‑150 (MQ=60)

                                                                                                          GTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCC                                            >  SRR19973074.2763045/1‑150 (MQ=60)

                                                                                                           TATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCA                                           >  SRR19973074.697064/1‑150 (MQ=60)

                                                                                                              CAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCA                                        <  SRR19973074.3304243/150‑1 (MQ=60)

                                                                                                               AAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAA                                       >  SRR19973074.3740874/1‑150 (MQ=60)

                                                                                                                 GACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATT                                     <  SRR19973074.505327/150‑1 (MQ=60)

                                                                                                                    GGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTT                                  <  SRR19973074.3966649/150‑1 (MQ=60)

                                                                                                                    GGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTT                                  <  SRR19973074.3966876/150‑1 (MQ=60)

                                                                                                                    GGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTT                                  <  SRR19973074.3966970/150‑1 (MQ=60)

                                                                                                                          GTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGG                            >  SRR19973074.806805/1‑150 (MQ=60)

                                                                                                                           TTGTTCGCGTGCGTTCTGAATTTCACTACGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTCAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGC                           <  SRR19973074.2633739/150‑1 (MQ=60)

                                                                                                                              TTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAG                        <  SRR19973074.3664951/150‑1 (MQ=60)

                                                                                                                                CGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTT                      <  SRR19973074.2441381/150‑1 (MQ=60)

                                                                                                                                CGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTT                      >  SRR19973074.1827591/1‑150 (MQ=60)

                                                                                                                                 GCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTG                     <  SRR19973074.3551480/150‑1 (MQ=60)

                                                                                                                                 GCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTG                     <  SRR19973074.2970261/150‑1 (MQ=60)

                                                                                                                                  CGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGC                    >  SRR19973074.803395/1‑150 (MQ=60)

                                                                                                                                     GCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGT                 <  SRR19973074.1569107/150‑1 (MQ=60)

                                                                                                                                      CGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTT                >  SRR19973074.3259464/1‑150 (MQ=60)

                                                                                                                                      CGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTT                <  SRR19973074.941291/150‑1 (MQ=60)

                                                                                                                                        TTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGT              >  SRR19973074.146219/1‑150 (MQ=60)

                                                                                                                                           TGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAAC           >  SRR19973074.538705/1‑150 (MQ=60)

                                                                                                                                           TGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAAC           <  SRR19973074.1645309/150‑1 (MQ=60)

                                                                                                                                           TGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAAC           >  SRR19973074.3880787/1‑150 (MQ=60)

                                                                                                                                           TGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAAC           >  SRR19973074.3880785/1‑150 (MQ=60)

                                                                                                                                            GAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACC          >  SRR19973074.2544430/1‑150 (MQ=60)

                                                                                                                                            GAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACC          <  SRR19973074.2053208/150‑1 (MQ=60)

                                                                                                                                             AATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCA         >  SRR19973074.2646385/1‑150 (MQ=60)

                                                                                                                                              ATTTCACTAAGCAGCGTATCCATCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCAG        >  SRR19973074.342818/1‑150 (MQ=60)

                                                                                                                                               TTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCAGC       <  SRR19973074.3161743/150‑1 (MQ=60)

                                                                                                                                                  CACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCAGCGTA    >  SRR19973074.2260111/1‑150 (MQ=60)

                                                                                                                                                  CACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCAGCGTA    >  SRR19973074.2260060/1‑150 (MQ=60)

                                                                                                                                                    CTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCAGCGTACC  >  SRR19973074.2367072/1‑150 (MQ=60)

ACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCAGCGTACC  >  CP009273/3395933‑3396230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`